{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,10]],"date-time":"2026-01-10T09:11:55Z","timestamp":1768036315481,"version":"3.49.0"},"reference-count":78,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2023,7,5]],"date-time":"2023-07-05T00:00:00Z","timestamp":1688515200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Health &amp; Welfare, Republic of Korea","award":["HI19C1132"],"award-info":[{"award-number":["HI19C1132"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>We recently developed a multiplex diagnostic kit, QPLEX\u2122 Alz plus assay kit, which captures amyloid-\u03b21-40, galectin-3 binding protein, angiotensin-converting enzyme, and periostin simultaneously using microliters of peripheral blood and utilizes an optimized algorithm for screening Alzheimer\u2019s disease (AD) by correlating with cerebral amyloid deposition. Owing to the demand for early AD detection, we investigate the potential of our kit for the early clinical diagnosis of AD. A total of 1395 participants were recruited, and their blood samples were analyzed with the QPLEX\u2122 kit. The average of QPLEX\u2122 algorithm values in each group increased gradually in the order of the clinical progression continuum of AD: cognitively normal (0.382 \u00b1 0.150), subjective cognitive decline (0.452 \u00b1 0.130), mild cognitive impairment (0.484 \u00b1 0.129), and AD (0.513 \u00b1 0.136). The algorithm values between each group showed statistically significant differences among groups divided by Mini-Mental State Examination and Clinical Dementia Rating. The QPLEX\u2122 algorithm values could be used to distinguish the clinical continuum of AD or cognitive function. Because blood-based diagnosis is more accessible, convenient, and cost- and time-effective than cerebral spinal fluid or positron emission tomography imaging-based diagnosis, the QPLEX\u2122 kit can potentially be used for health checkups and the early clinical diagnosis of AD.<\/jats:p>","DOI":"10.3390\/ijms241311119","type":"journal-article","created":{"date-parts":[[2023,7,6]],"date-time":"2023-07-06T00:34:30Z","timestamp":1688603670000},"page":"11119","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["The QPLEX\u2122 Plus Assay Kit for the Early Clinical Diagnosis of Alzheimer\u2019s Disease"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9792-3570","authenticated-orcid":false,"given":"Hunjong","family":"Na","sequence":"first","affiliation":[{"name":"Department of Electrical and Computer Engineering, Seoul National University, Seoul 08826, Republic of Korea"},{"name":"QuantaMatrix Inc., Seoul 08506, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ki Young","family":"Shin","sequence":"additional","affiliation":[{"name":"Bio-MAX Institute, Seoul National University, Seoul 08826, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dokyung","family":"Lee","sequence":"additional","affiliation":[{"name":"QuantaMatrix Inc., Seoul 08506, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Changsik","family":"Yoon","sequence":"additional","affiliation":[{"name":"QuantaMatrix Inc., Seoul 08506, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sun-Ho","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Biomedical Sciences, College of Medicine, Seoul National University, Seoul 03080, Republic of Korea"},{"name":"Neuroscience Research Institute, Medical Research Center, College of Medicine, Seoul National University, Seoul 03080, Republic of Korea"},{"name":"SNU Dementia Research Center, College of Medicine, Seoul National University, Seoul 03080, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jong-Chan","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Biomedical Sciences, College of Medicine, Seoul National University, Seoul 03080, Republic of Korea"},{"name":"Neuroscience Research Institute, Medical Research Center, College of Medicine, Seoul National University, Seoul 03080, Republic of Korea"},{"name":"SNU Dementia Research Center, College of Medicine, Seoul National University, Seoul 03080, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Inhee","family":"Mook-Jung","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Biomedical Sciences, College of Medicine, Seoul National University, Seoul 03080, Republic of Korea"},{"name":"Neuroscience Research Institute, Medical Research Center, College of Medicine, Seoul National University, Seoul 03080, Republic of Korea"},{"name":"SNU Dementia Research Center, College of Medicine, Seoul National University, Seoul 03080, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jisung","family":"Jang","sequence":"additional","affiliation":[{"name":"QuantaMatrix Inc., Seoul 08506, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sunghoon","family":"Kwon","sequence":"additional","affiliation":[{"name":"Department of Electrical and Computer Engineering, Seoul National University, Seoul 08826, Republic of Korea"},{"name":"QuantaMatrix Inc., Seoul 08506, Republic of Korea"},{"name":"Bio-MAX Institute, Seoul National University, Seoul 08826, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,7,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/S1474-4422(12)70291-0","article-title":"Tracking pathophysiological processes in Alzheimer\u2019s disease: An updated hypothetical model of dynamic biomarkers","volume":"12","author":"Jack","year":"2013","journal-title":"Lancet Neurol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1016\/S1474-4422(13)70044-9","article-title":"Amyloid beta deposition, neurodegeneration, and cognitive decline in sporadic Alzheimer\u2019s disease: A prospective cohort study","volume":"12","author":"Villemagne","year":"2013","journal-title":"Lancet Neurol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1073","DOI":"10.5455\/njppp.2019.9.0828114082019","article-title":"Drug prescription patterns in patients with Alzheimer\u2019s disease in an urban Neuro-speciality clinic in Western India","volume":"9","author":"Joshi","year":"2019","journal-title":"Natl. 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