{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,21]],"date-time":"2026-01-21T04:21:13Z","timestamp":1768969273439,"version":"3.49.0"},"reference-count":65,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2023,7,7]],"date-time":"2023-07-07T00:00:00Z","timestamp":1688688000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"mprovement of Higher Education Personnel\u2013CAPES","award":["E-26\/201.406\/2021"],"award-info":[{"award-number":["E-26\/201.406\/2021"]}]},{"name":"mprovement of Higher Education Personnel\u2013CAPES","award":["VPPCB-005-FIO-20-2-56"],"award-info":[{"award-number":["VPPCB-005-FIO-20-2-56"]}]},{"name":"mprovement of Higher Education Personnel\u2013CAPES","award":["E-26\/210.254\/2020"],"award-info":[{"award-number":["E-26\/210.254\/2020"]}]},{"name":"mprovement of Higher Education Personnel\u2013CAPES","award":["E-26\/210.657\/2021"],"award-info":[{"award-number":["E-26\/210.657\/2021"]}]},{"name":"mprovement of Higher Education Personnel\u2013CAPES","award":["E-26\/210.273\/2018"],"award-info":[{"award-number":["E-26\/210.273\/2018"]}]},{"name":"mprovement of Higher Education Personnel\u2013CAPES","award":["E-26\/201.040\/2021"],"award-info":[{"award-number":["E-26\/201.040\/2021"]}]},{"name":"mprovement of Higher Education Personnel\u2013CAPES","award":["E-26\/210.817\/2021"],"award-info":[{"award-number":["E-26\/210.817\/2021"]}]},{"name":"mprovement of Higher Education Personnel\u2013CAPES","award":["403008\/2021-2"],"award-info":[{"award-number":["403008\/2021-2"]}]},{"name":"Funda\u00e7\u00e3o Carlos Chagas Filho de Amparo \u00e0 Pesquisa do Estado do Rio de Janeiro (FAPERJ)","award":["E-26\/201.406\/2021"],"award-info":[{"award-number":["E-26\/201.406\/2021"]}]},{"name":"Funda\u00e7\u00e3o Carlos Chagas Filho de Amparo \u00e0 Pesquisa do Estado do Rio de Janeiro (FAPERJ)","award":["VPPCB-005-FIO-20-2-56"],"award-info":[{"award-number":["VPPCB-005-FIO-20-2-56"]}]},{"name":"Funda\u00e7\u00e3o Carlos Chagas Filho de Amparo \u00e0 Pesquisa do Estado do Rio de Janeiro (FAPERJ)","award":["E-26\/210.254\/2020"],"award-info":[{"award-number":["E-26\/210.254\/2020"]}]},{"name":"Funda\u00e7\u00e3o Carlos Chagas Filho de Amparo \u00e0 Pesquisa do Estado do Rio de Janeiro (FAPERJ)","award":["E-26\/210.657\/2021"],"award-info":[{"award-number":["E-26\/210.657\/2021"]}]},{"name":"Funda\u00e7\u00e3o Carlos Chagas Filho de Amparo \u00e0 Pesquisa do Estado do Rio de Janeiro (FAPERJ)","award":["E-26\/210.273\/2018"],"award-info":[{"award-number":["E-26\/210.273\/2018"]}]},{"name":"Funda\u00e7\u00e3o Carlos Chagas Filho de Amparo \u00e0 Pesquisa do Estado do Rio de Janeiro (FAPERJ)","award":["E-26\/201.040\/2021"],"award-info":[{"award-number":["E-26\/201.040\/2021"]}]},{"name":"Funda\u00e7\u00e3o Carlos Chagas Filho de Amparo \u00e0 Pesquisa do Estado do Rio de Janeiro (FAPERJ)","award":["E-26\/210.817\/2021"],"award-info":[{"award-number":["E-26\/210.817\/2021"]}]},{"name":"Funda\u00e7\u00e3o Carlos Chagas Filho de Amparo \u00e0 Pesquisa do Estado do Rio de Janeiro (FAPERJ)","award":["403008\/2021-2"],"award-info":[{"award-number":["403008\/2021-2"]}]},{"name":"Oswaldo Cruz Institute","award":["E-26\/201.406\/2021"],"award-info":[{"award-number":["E-26\/201.406\/2021"]}]},{"name":"Oswaldo Cruz Institute","award":["VPPCB-005-FIO-20-2-56"],"award-info":[{"award-number":["VPPCB-005-FIO-20-2-56"]}]},{"name":"Oswaldo Cruz Institute","award":["E-26\/210.254\/2020"],"award-info":[{"award-number":["E-26\/210.254\/2020"]}]},{"name":"Oswaldo Cruz Institute","award":["E-26\/210.657\/2021"],"award-info":[{"award-number":["E-26\/210.657\/2021"]}]},{"name":"Oswaldo Cruz Institute","award":["E-26\/210.273\/2018"],"award-info":[{"award-number":["E-26\/210.273\/2018"]}]},{"name":"Oswaldo Cruz Institute","award":["E-26\/201.040\/2021"],"award-info":[{"award-number":["E-26\/201.040\/2021"]}]},{"name":"Oswaldo Cruz Institute","award":["E-26\/210.817\/2021"],"award-info":[{"award-number":["E-26\/210.817\/2021"]}]},{"name":"Oswaldo Cruz Institute","award":["403008\/2021-2"],"award-info":[{"award-number":["403008\/2021-2"]}]},{"name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico (CNPq)","award":["E-26\/201.406\/2021"],"award-info":[{"award-number":["E-26\/201.406\/2021"]}]},{"name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico (CNPq)","award":["VPPCB-005-FIO-20-2-56"],"award-info":[{"award-number":["VPPCB-005-FIO-20-2-56"]}]},{"name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico (CNPq)","award":["E-26\/210.254\/2020"],"award-info":[{"award-number":["E-26\/210.254\/2020"]}]},{"name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico (CNPq)","award":["E-26\/210.657\/2021"],"award-info":[{"award-number":["E-26\/210.657\/2021"]}]},{"name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico (CNPq)","award":["E-26\/210.273\/2018"],"award-info":[{"award-number":["E-26\/210.273\/2018"]}]},{"name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico (CNPq)","award":["E-26\/201.040\/2021"],"award-info":[{"award-number":["E-26\/201.040\/2021"]}]},{"name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico (CNPq)","award":["E-26\/210.817\/2021"],"award-info":[{"award-number":["E-26\/210.817\/2021"]}]},{"name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico (CNPq)","award":["403008\/2021-2"],"award-info":[{"award-number":["403008\/2021-2"]}]},{"DOI":"10.13039\/501100004586","name":"FAPERJ","doi-asserted-by":"publisher","award":["E-26\/201.406\/2021"],"award-info":[{"award-number":["E-26\/201.406\/2021"]}],"id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004586","name":"FAPERJ","doi-asserted-by":"publisher","award":["VPPCB-005-FIO-20-2-56"],"award-info":[{"award-number":["VPPCB-005-FIO-20-2-56"]}],"id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004586","name":"FAPERJ","doi-asserted-by":"publisher","award":["E-26\/210.254\/2020"],"award-info":[{"award-number":["E-26\/210.254\/2020"]}],"id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004586","name":"FAPERJ","doi-asserted-by":"publisher","award":["E-26\/210.657\/2021"],"award-info":[{"award-number":["E-26\/210.657\/2021"]}],"id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004586","name":"FAPERJ","doi-asserted-by":"publisher","award":["E-26\/210.273\/2018"],"award-info":[{"award-number":["E-26\/210.273\/2018"]}],"id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004586","name":"FAPERJ","doi-asserted-by":"publisher","award":["E-26\/201.040\/2021"],"award-info":[{"award-number":["E-26\/201.040\/2021"]}],"id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004586","name":"FAPERJ","doi-asserted-by":"publisher","award":["E-26\/210.817\/2021"],"award-info":[{"award-number":["E-26\/210.817\/2021"]}],"id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004586","name":"FAPERJ","doi-asserted-by":"publisher","award":["403008\/2021-2"],"award-info":[{"award-number":["403008\/2021-2"]}],"id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006507","name":"MS\/Programa Inova FIOCRUZ\u2013Edital Gera\u00e7\u00e3o de Conhecimento\u2013Enfrentamento da Pandemia e P\u00f3s-pandemia COVID-19: Encomendas Estrat\u00e9gicas\u201d\/processo","doi-asserted-by":"publisher","award":["E-26\/201.406\/2021"],"award-info":[{"award-number":["E-26\/201.406\/2021"]}],"id":[{"id":"10.13039\/501100006507","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006507","name":"MS\/Programa Inova FIOCRUZ\u2013Edital Gera\u00e7\u00e3o de Conhecimento\u2013Enfrentamento da Pandemia e P\u00f3s-pandemia COVID-19: Encomendas Estrat\u00e9gicas\u201d\/processo","doi-asserted-by":"publisher","award":["VPPCB-005-FIO-20-2-56"],"award-info":[{"award-number":["VPPCB-005-FIO-20-2-56"]}],"id":[{"id":"10.13039\/501100006507","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006507","name":"MS\/Programa Inova FIOCRUZ\u2013Edital Gera\u00e7\u00e3o de Conhecimento\u2013Enfrentamento da Pandemia e P\u00f3s-pandemia COVID-19: Encomendas Estrat\u00e9gicas\u201d\/processo","doi-asserted-by":"publisher","award":["E-26\/210.254\/2020"],"award-info":[{"award-number":["E-26\/210.254\/2020"]}],"id":[{"id":"10.13039\/501100006507","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006507","name":"MS\/Programa Inova FIOCRUZ\u2013Edital Gera\u00e7\u00e3o de Conhecimento\u2013Enfrentamento da Pandemia e P\u00f3s-pandemia COVID-19: Encomendas Estrat\u00e9gicas\u201d\/processo","doi-asserted-by":"publisher","award":["E-26\/210.657\/2021"],"award-info":[{"award-number":["E-26\/210.657\/2021"]}],"id":[{"id":"10.13039\/501100006507","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006507","name":"MS\/Programa Inova FIOCRUZ\u2013Edital Gera\u00e7\u00e3o de Conhecimento\u2013Enfrentamento da Pandemia e P\u00f3s-pandemia COVID-19: Encomendas Estrat\u00e9gicas\u201d\/processo","doi-asserted-by":"publisher","award":["E-26\/210.273\/2018"],"award-info":[{"award-number":["E-26\/210.273\/2018"]}],"id":[{"id":"10.13039\/501100006507","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006507","name":"MS\/Programa Inova FIOCRUZ\u2013Edital Gera\u00e7\u00e3o de Conhecimento\u2013Enfrentamento da Pandemia e P\u00f3s-pandemia COVID-19: Encomendas Estrat\u00e9gicas\u201d\/processo","doi-asserted-by":"publisher","award":["E-26\/201.040\/2021"],"award-info":[{"award-number":["E-26\/201.040\/2021"]}],"id":[{"id":"10.13039\/501100006507","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006507","name":"MS\/Programa Inova FIOCRUZ\u2013Edital Gera\u00e7\u00e3o de Conhecimento\u2013Enfrentamento da Pandemia e P\u00f3s-pandemia COVID-19: Encomendas Estrat\u00e9gicas\u201d\/processo","doi-asserted-by":"publisher","award":["E-26\/210.817\/2021"],"award-info":[{"award-number":["E-26\/210.817\/2021"]}],"id":[{"id":"10.13039\/501100006507","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006507","name":"MS\/Programa Inova FIOCRUZ\u2013Edital Gera\u00e7\u00e3o de Conhecimento\u2013Enfrentamento da Pandemia e P\u00f3s-pandemia COVID-19: Encomendas Estrat\u00e9gicas\u201d\/processo","doi-asserted-by":"publisher","award":["403008\/2021-2"],"award-info":[{"award-number":["403008\/2021-2"]}],"id":[{"id":"10.13039\/501100006507","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004586","name":"FAPERJ","doi-asserted-by":"publisher","award":["E-26\/201.406\/2021"],"award-info":[{"award-number":["E-26\/201.406\/2021"]}],"id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004586","name":"FAPERJ","doi-asserted-by":"publisher","award":["VPPCB-005-FIO-20-2-56"],"award-info":[{"award-number":["VPPCB-005-FIO-20-2-56"]}],"id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004586","name":"FAPERJ","doi-asserted-by":"publisher","award":["E-26\/210.254\/2020"],"award-info":[{"award-number":["E-26\/210.254\/2020"]}],"id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004586","name":"FAPERJ","doi-asserted-by":"publisher","award":["E-26\/210.657\/2021"],"award-info":[{"award-number":["E-26\/210.657\/2021"]}],"id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004586","name":"FAPERJ","doi-asserted-by":"publisher","award":["E-26\/210.273\/2018"],"award-info":[{"award-number":["E-26\/210.273\/2018"]}],"id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004586","name":"FAPERJ","doi-asserted-by":"publisher","award":["E-26\/201.040\/2021"],"award-info":[{"award-number":["E-26\/201.040\/2021"]}],"id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004586","name":"FAPERJ","doi-asserted-by":"publisher","award":["E-26\/210.817\/2021"],"award-info":[{"award-number":["E-26\/210.817\/2021"]}],"id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004586","name":"FAPERJ","doi-asserted-by":"publisher","award":["403008\/2021-2"],"award-info":[{"award-number":["403008\/2021-2"]}],"id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004586","name":"Edital Segunda Chamada Emergencial de Projetos para Combater os Efeitos da COVID-19\u20132020","doi-asserted-by":"publisher","award":["E-26\/201.406\/2021"],"award-info":[{"award-number":["E-26\/201.406\/2021"]}],"id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004586","name":"Edital Segunda Chamada Emergencial de Projetos para Combater os Efeitos da COVID-19\u20132020","doi-asserted-by":"publisher","award":["VPPCB-005-FIO-20-2-56"],"award-info":[{"award-number":["VPPCB-005-FIO-20-2-56"]}],"id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004586","name":"Edital Segunda Chamada Emergencial de Projetos para Combater os Efeitos da COVID-19\u20132020","doi-asserted-by":"publisher","award":["E-26\/210.254\/2020"],"award-info":[{"award-number":["E-26\/210.254\/2020"]}],"id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004586","name":"Edital Segunda Chamada Emergencial de Projetos para Combater os Efeitos da COVID-19\u20132020","doi-asserted-by":"publisher","award":["E-26\/210.657\/2021"],"award-info":[{"award-number":["E-26\/210.657\/2021"]}],"id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004586","name":"Edital Segunda Chamada Emergencial de Projetos para Combater os Efeitos da COVID-19\u20132020","doi-asserted-by":"publisher","award":["E-26\/210.273\/2018"],"award-info":[{"award-number":["E-26\/210.273\/2018"]}],"id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004586","name":"Edital Segunda Chamada Emergencial de Projetos para Combater os Efeitos da COVID-19\u20132020","doi-asserted-by":"publisher","award":["E-26\/201.040\/2021"],"award-info":[{"award-number":["E-26\/201.040\/2021"]}],"id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004586","name":"Edital Segunda Chamada Emergencial de Projetos para Combater os Efeitos da COVID-19\u20132020","doi-asserted-by":"publisher","award":["E-26\/210.817\/2021"],"award-info":[{"award-number":["E-26\/210.817\/2021"]}],"id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004586","name":"Edital Segunda Chamada Emergencial de Projetos para Combater os Efeitos da COVID-19\u20132020","doi-asserted-by":"publisher","award":["403008\/2021-2"],"award-info":[{"award-number":["403008\/2021-2"]}],"id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004586","name":"FAPERJ","doi-asserted-by":"publisher","award":["E-26\/201.406\/2021"],"award-info":[{"award-number":["E-26\/201.406\/2021"]}],"id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004586","name":"FAPERJ","doi-asserted-by":"publisher","award":["VPPCB-005-FIO-20-2-56"],"award-info":[{"award-number":["VPPCB-005-FIO-20-2-56"]}],"id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004586","name":"FAPERJ","doi-asserted-by":"publisher","award":["E-26\/210.254\/2020"],"award-info":[{"award-number":["E-26\/210.254\/2020"]}],"id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004586","name":"FAPERJ","doi-asserted-by":"publisher","award":["E-26\/210.657\/2021"],"award-info":[{"award-number":["E-26\/210.657\/2021"]}],"id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004586","name":"FAPERJ","doi-asserted-by":"publisher","award":["E-26\/210.273\/2018"],"award-info":[{"award-number":["E-26\/210.273\/2018"]}],"id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004586","name":"FAPERJ","doi-asserted-by":"publisher","award":["E-26\/201.040\/2021"],"award-info":[{"award-number":["E-26\/201.040\/2021"]}],"id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004586","name":"FAPERJ","doi-asserted-by":"publisher","award":["E-26\/210.817\/2021"],"award-info":[{"award-number":["E-26\/210.817\/2021"]}],"id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004586","name":"FAPERJ","doi-asserted-by":"publisher","award":["403008\/2021-2"],"award-info":[{"award-number":["403008\/2021-2"]}],"id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Edital Afec\u00e7\u00f5es p\u00f3s-COVID","award":["E-26\/201.406\/2021"],"award-info":[{"award-number":["E-26\/201.406\/2021"]}]},{"name":"Edital Afec\u00e7\u00f5es p\u00f3s-COVID","award":["VPPCB-005-FIO-20-2-56"],"award-info":[{"award-number":["VPPCB-005-FIO-20-2-56"]}]},{"name":"Edital Afec\u00e7\u00f5es p\u00f3s-COVID","award":["E-26\/210.254\/2020"],"award-info":[{"award-number":["E-26\/210.254\/2020"]}]},{"name":"Edital Afec\u00e7\u00f5es p\u00f3s-COVID","award":["E-26\/210.657\/2021"],"award-info":[{"award-number":["E-26\/210.657\/2021"]}]},{"name":"Edital Afec\u00e7\u00f5es p\u00f3s-COVID","award":["E-26\/210.273\/2018"],"award-info":[{"award-number":["E-26\/210.273\/2018"]}]},{"name":"Edital Afec\u00e7\u00f5es p\u00f3s-COVID","award":["E-26\/201.040\/2021"],"award-info":[{"award-number":["E-26\/201.040\/2021"]}]},{"name":"Edital Afec\u00e7\u00f5es p\u00f3s-COVID","award":["E-26\/210.817\/2021"],"award-info":[{"award-number":["E-26\/210.817\/2021"]}]},{"name":"Edital Afec\u00e7\u00f5es p\u00f3s-COVID","award":["403008\/2021-2"],"award-info":[{"award-number":["403008\/2021-2"]}]},{"DOI":"10.13039\/501100003593","name":"CNPq","doi-asserted-by":"publisher","award":["E-26\/201.406\/2021"],"award-info":[{"award-number":["E-26\/201.406\/2021"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"CNPq","doi-asserted-by":"publisher","award":["VPPCB-005-FIO-20-2-56"],"award-info":[{"award-number":["VPPCB-005-FIO-20-2-56"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"CNPq","doi-asserted-by":"publisher","award":["E-26\/210.254\/2020"],"award-info":[{"award-number":["E-26\/210.254\/2020"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"CNPq","doi-asserted-by":"publisher","award":["E-26\/210.657\/2021"],"award-info":[{"award-number":["E-26\/210.657\/2021"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"CNPq","doi-asserted-by":"publisher","award":["E-26\/210.273\/2018"],"award-info":[{"award-number":["E-26\/210.273\/2018"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"CNPq","doi-asserted-by":"publisher","award":["E-26\/201.040\/2021"],"award-info":[{"award-number":["E-26\/201.040\/2021"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"CNPq","doi-asserted-by":"publisher","award":["E-26\/210.817\/2021"],"award-info":[{"award-number":["E-26\/210.817\/2021"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"CNPq","doi-asserted-by":"publisher","award":["403008\/2021-2"],"award-info":[{"award-number":["403008\/2021-2"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Human herpesviruses (HHVs) can establish latency and be reactivated, also are neurotropic viruses that can trigger neurological disorders. HHV-6 is a herpesvirus that is associated with neurological disorders. Studies have reported the detection of HHV-6 in patients with COVID-19 and neurological manifestations. However, specific diagnoses of the neurological disorders caused by these viruses tend to be invasive or difficult to interpret. This study aimed to establish a relationship between miRNA and neurological manifestations in patients co-infected with COVID-19 and HHV-6 and evaluate miRNAs as potential biomarkers. Serum samples from COVID-19 patients in the three cohorts were analyzed. miRNA analysis by real-time polymerase chain reaction (qPCR) revealed miRNAs associated with neuroinflammation were highly expressed in patients with neurological disorders and HHV-6 detection. When compared with the group of patients without detection of HHVs DNA and without neurological alterations, the group with detection of HHV-6 DNA and neurological alteration, displayed significant differences in the expression of mir-21, mir-146a, miR-155 and miR-let-7b (p &lt; 0.01). Our results reinforce the involvement of miRNAs in neurological disorders and provide insights into their use as biomarkers for neurological disorders triggered by HHV-6. Furthermore, understanding the expression of miRNAs may contribute to therapeutic strategies.<\/jats:p>","DOI":"10.3390\/ijms241311201","type":"journal-article","created":{"date-parts":[[2023,7,10]],"date-time":"2023-07-10T00:35:21Z","timestamp":1688949321000},"page":"11201","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["miRNAs in Neurological Manifestation in Patients Co-Infected with SARS-CoV-2 and Herpesv\u00edrus 6 (HHV-6)"],"prefix":"10.3390","volume":"24","author":[{"given":"Vanessa Cristine de Souza","family":"Carneiro","sequence":"first","affiliation":[{"name":"Laboratory of Molecular Virology and Parasitology, Oswaldo Cruz Institute, Fiocruz, Rio de Janeiro 21040-360, Brazil"},{"name":"Laboratory of Technological Development in Virology, Oswaldo Cruz Institute, Fiocruz, Rio de Janeiro 21040-360, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5340-8697","authenticated-orcid":false,"given":"Otacilio da Cruz","family":"Moreira","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Virology and Parasitology, Oswaldo Cruz Institute, Fiocruz, Rio de Janeiro 21040-360, Brazil"},{"name":"Real Time PCR Platform RPT09A, Fiocruz, Rio de Janeiro 21040-360, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wagner Luis da Costa Nunes Pimentel","family":"Coelho","sequence":"additional","affiliation":[{"name":"Laboratory of Technological Development in Virology, Oswaldo Cruz Institute, Fiocruz, Rio de Janeiro 21040-360, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beatriz Chan","family":"Rio","sequence":"additional","affiliation":[{"name":"Laboratory of Technological Development in Virology, Oswaldo Cruz Institute, Fiocruz, Rio de Janeiro 21040-360, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7972-9141","authenticated-orcid":false,"given":"Dmitry Jos\u00e9 de Santana","family":"Sarmento","sequence":"additional","affiliation":[{"name":"Department of Oral Diagnosis, School of Dentistry, State University of Para\u00edba, Araruna 58429-500, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andreza Lemos","family":"Salvio","sequence":"additional","affiliation":[{"name":"Laboratory of Translacional Neurosciences, Biomedical Institute, Federal University of the State of Rio de Janeiro-UNIRIO, Rio de Janeiro 22290-240, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1538-6730","authenticated-orcid":false,"given":"Soniza Vieira","family":"Alves-Leon","sequence":"additional","affiliation":[{"name":"Laboratory of Translacional Neurosciences, Biomedical Institute, Federal University of the State of Rio de Janeiro-UNIRIO, Rio de Janeiro 22290-240, Brazil"},{"name":"Department of Neurology, Reference and Research Center for Multiple Sclerosis and Other Central Nervous System Idiopathic Demyelinating Inflammatory Diseases, Clementino Fraga Filho University Hospital, Federal University of Rio de Janeiro, Rio de Janeiro 21941-617, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vanessa Salete","family":"de Paula","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Virology and Parasitology, Oswaldo Cruz Institute, Fiocruz, Rio de Janeiro 21040-360, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luciane Almeida Amado","family":"Leon","sequence":"additional","affiliation":[{"name":"Laboratory of Technological Development in Virology, Oswaldo Cruz Institute, Fiocruz, Rio de Janeiro 21040-360, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,7,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/j.virusres.2017.01.019","article-title":"Herpesvirus DNA polymerases: Structures, functions and inhibitors","volume":"234","author":"Zarrouk","year":"2017","journal-title":"Virus Res."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Grinde, B. (2013). Herpesviruses: Latency and reactivation\u2014Viral strategies and host response. J. Oral Microbiol., 5.","DOI":"10.3402\/jom.v5i0.22766"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"702","DOI":"10.1016\/S0140-6736(64)91524-7","article-title":"Virus particles in cultured lymphoblasts from Burkitt\u2019s Lymphoma","volume":"1","author":"Epstein","year":"1964","journal-title":"Lancet"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"de Oliveira Lopes, A., do Nascimento Marinho, P., d\u2019Ambrosio de Souza Medeiros, L., and de Paula, V.S. (2022). Human Gammaherpesvirus 8 Oncogenes Associated with Kaposi\u2019s Sarcoma. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23137203"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1038\/ncpneuro0401","article-title":"Herpesvirus infections of the nervous system","volume":"3","author":"Gilden","year":"2007","journal-title":"Nat. Clin. Pract. Neurol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"e220200","DOI":"10.1590\/0074-02760220200","article-title":"Family Herpesviridae and neuroinfections: Current status and research in progress","volume":"117","author":"Carneiro","year":"2022","journal-title":"Mem. Inst. Oswaldo Cruz"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"e942","DOI":"10.1212\/NXI.0000000000000942","article-title":"Human Herpesvirus 6 Encephalitis in Immunocompetent and Immunocompromised Hosts","volume":"8","author":"Berzero","year":"2021","journal-title":"Neurol. Neuroimmunol. Neuroinflamm."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.eplepsyres.2019.03.016","article-title":"Infection with HHV-6 and its role in epilepsy","volume":"153","author":"Bartolini","year":"2019","journal-title":"Epilepsy Res."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"211","DOI":"10.4103\/1673-5374.226380","article-title":"Contributions of neurotropic human herpesviruses herpes simplex virus 1 and human herpesvirus 6 to neurodegenerative disease pathology","volume":"13","author":"Hogestyn","year":"2018","journal-title":"Neural Regen. Res."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1007\/s10238-021-00751-7","article-title":"COVID-19-associated opportunistic infections: A snapshot on the current reports","volume":"22","author":"Abdoli","year":"2022","journal-title":"Clin. Exp. Med."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1016\/j.stemcr.2021.12.011","article-title":"The blood-brain barrier is dysregulated in COVID-19 and serves as a CNS entry route for SARS-CoV-2","volume":"17","author":"Krasemann","year":"2022","journal-title":"Stem Cell Rep."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1637","DOI":"10.1212\/WNL.0b013e3182a9f531","article-title":"Herpes simplex virus-1 encephalitis can trigger anti-NMDA receptor encephalitis: Case report","volume":"81","author":"Leypoldt","year":"2013","journal-title":"Neurology"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"3995","DOI":"10.1007\/s10072-021-05506-6","article-title":"Anti-GAD associated post-infectious cerebellitis after COVID-19 infection","volume":"42","author":"Emekli","year":"2021","journal-title":"Neurol. Sci."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Durovic, E., Bien, C., Bien, C.G., and Isenmann, S. (2021). MOG antibody-associated encephalitis secondary to COVID-19: Case report. BMC Neurol., 21.","DOI":"10.1186\/s12883-021-02449-5"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"845755","DOI":"10.3389\/fneur.2022.845755","article-title":"MOG Antibody-Associated Disorders Following SARS-CoV-2 Vaccination: A Case Report and Literature Review","volume":"13","author":"Matsumoto","year":"2022","journal-title":"Front. Neurol."},{"key":"ref_16","first-page":"687","article-title":"Human herpes 6 encephalitis in co-infection with COVID-19","volume":"123","author":"Pizzo","year":"2022","journal-title":"Acta Neurol. Belg."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"577521","DOI":"10.1016\/j.jneuroim.2021.577521","article-title":"COVID-19, HHV6 and MOG antibody: A perfect storm","volume":"353","author":"Jumah","year":"2021","journal-title":"J. Neuroimmunol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1186\/s12985-022-01828-9","article-title":"Herpesvirus and neurological manifestations in patients with severe coronavirus disease","volume":"19","author":"Carneiro","year":"2022","journal-title":"Virol. J."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1212","DOI":"10.1002\/jmv.27392","article-title":"Presence and clinical impact of human herpesvirus-6 infection in patients with moderate to critical coronavirus disease-19","volume":"3","author":"Lino","year":"2022","journal-title":"J. Med. Virol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1785801","DOI":"10.1080\/20002297.2020.1785801","article-title":"Longitudinal study on oral shedding of human betaherpesviruses 6 and 7 in renal transplant recipients reveals active replication","volume":"12","author":"Raposo","year":"2020","journal-title":"J. Oral Microbiol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1086\/589747","article-title":"The management of encephalitis: Clinical practice guidelines by the Infectious Diseases Society of America","volume":"47","author":"Tunkel","year":"2008","journal-title":"Clin. Infect. Dis."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1718","DOI":"10.4102\/ajlm.v11i1.1718","article-title":"Human herpes virus type-6 is associated with central nervous system infections in children in Sudan","volume":"11","author":"Abdelrahim","year":"2022","journal-title":"Afr. J. Lab. Med."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"e00313-20","DOI":"10.1128\/JCM.00313-20","article-title":"Pathogen or Bystander: Clinical Significance of Detecting Human Herpesvirus 6 in Pediatric Cerebrospinal Fluid","volume":"58","author":"Pandey","year":"2020","journal-title":"J. Clin. Microbiol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1298","DOI":"10.1128\/JCM.02115-06","article-title":"Human herpesvirus 6 DNA levels in cerebrospinal fluid due to primary infection differ from those due to chromosomal viral integration and have implications for diagnosis of encephalitis","volume":"45","author":"Ward","year":"2007","journal-title":"J. Clin. Microbiol."},{"key":"ref_25","first-page":"e2822020","article-title":"Cerebrospinal fluid: History, c.t., indications, contraindications and complications. L\u00edquido cefalorraquidiano: Hist\u00f3ria, t\u00e9cnicas de coleta, indica\u00e7\u00f5es, contraindica\u00e7\u00f5es e complica\u00e7\u00f5es","volume":"56","author":"Oliveira","year":"2020","journal-title":"J. Bras. Patol. Med. Lab."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1016\/j.brainres.2010.03.103","article-title":"MicroRNAs as biomarkers for CNS cancer and other disorders","volume":"1338","author":"Ferretti","year":"2010","journal-title":"Brain Res."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1182","DOI":"10.3389\/fimmu.2017.01182","article-title":"The Clinical Application of MicroRNAs in Infectious Disease","volume":"8","author":"Drury","year":"2017","journal-title":"Front. Immunol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"5003","DOI":"10.1073\/pnas.1019055108","article-title":"Argonaute2 complexes carry a population of circulating microRNAs independent of vesicles in human plasma","volume":"108","author":"Arroyo","year":"2011","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Burgos, K., Malenica, I., Metpally, R., Courtright, A., Rakela, B., Beach, T., Shill, H., Adler, C., Sabbagh, M., and Villa, S. (2014). Profiles of extracellular miRNA in cerebrospinal fluid and serum from patients with Alzheimer\u2019s and Parkinson\u2019s diseases correlate with disease status and features of pathology. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0094839"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"227","DOI":"10.3389\/fphys.2019.00227","article-title":"Emerging Roles of miRNAs in Brain Development and Perinatal Brain Injury","volume":"10","author":"Cho","year":"2019","journal-title":"Front. Physiol."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Zingale, V.D., Gugliandolo, A., and Mazzon, E. (2021). MiR-155: An Important Regulator of Neuroinflammation. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23010090"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1111\/j.1365-2567.2011.03514.x","article-title":"miR-155 modulates microglia-mediated immune response by down-regulating SOCS-1 and promoting cytokine and nitric oxide production","volume":"135","author":"Cardoso","year":"2012","journal-title":"Immunology"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"19","DOI":"10.3389\/fimmu.2015.00019","article-title":"Turning 21: Induction of miR-21 as a Key Switch in the Inflammatory Response","volume":"6","author":"Sheedy","year":"2015","journal-title":"Front. Immunol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1038\/jcbfm.2014.207","article-title":"Brain endothelial miR-146a negatively modulates T-cell adhesion through repressing multiple targets to inhibit NF-\u03baB activation","volume":"35","author":"Wu","year":"2015","journal-title":"J. Cereb. Blood Flow Metab."},{"key":"ref_35","unstructured":"Keikha, R., and Jebali, A. (Neurologia, 2021). Los biomarcadores neuroinflamatorios miARN en pacientes con COVID-19 con diferente gravedad de la enfermedad [The miRNA neuroinflammatory biomarkers in COVID-19 patients with different severity of illness], Neurologia, in press."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1177\/1073858417721150","article-title":"MicroRNAs: Roles in Regulating Neuroinflammation","volume":"24","author":"Gaudet","year":"2018","journal-title":"Neuroscientist"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"827","DOI":"10.1038\/nn.3113","article-title":"An unconventional role for miRNA: Let-7 activates Toll-like receptor 7 and causes neurodegeneration","volume":"15","author":"Lehmann","year":"2012","journal-title":"Nat. Neurosci."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/j.trsl.2021.05.004","article-title":"Circulating microRNA profiles predict the severity of COVID-19 in hospitalized patients","volume":"236","author":"Pinilla","year":"2021","journal-title":"Transl. Res."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1093\/cvr\/cvab338","article-title":"Association of cardiometabolic microRNAs with COVID-19 severity and mortality","volume":"118","author":"Gutmann","year":"2022","journal-title":"Cardiovasc. Res."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Toor, S.M., Aldous, E.K., Parray, A., Akhtar, N., Al-Sarraj, Y., Abdelalim, E.M., Arredouani, A., El-Agnaf, O., Thornalley, P.J., and Pananchikkal, S.V. (2022). Circulating MicroRNA Profiling Identifies Distinct MicroRNA Signatures in Acute Ischemic Stroke and Transient Ischemic Attack Patients. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24010108"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"40","DOI":"10.3390\/neurolint15010004","article-title":"miR-21, miR-93, miR-191, miR-let-7b, and miR-499 Expression Level in Plasma and Cerebrospinal Fluid in Patients with Prolonged Disorders of Consciousness","volume":"15","author":"Petrova","year":"2022","journal-title":"Neurol. Int."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Abe, A., Tanaka, M., Yasuoka, A., Saito, Y., Okada, S., Mishina, M., Abe, K., Kimura, K., and Asakura, T. (2020). Changes in Whole-Blood microRNA Profiles during the Onset and Treatment Process of Cerebral Infarction: A Human Study. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21093107"},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Vojdani, A., Vojdani, E., Saidara, E., and Maes, M. (2023). Persistent SARS-CoV-2 Infection, EBV, HHV-6 and Other Factors May Contribute to Inflammation and Autoimmunity in Long COVID. Viruses, 15.","DOI":"10.3390\/v15020400"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"468","DOI":"10.1002\/ejhf.2096","article-title":"Circulating cardiovascular microRNAs in critically ill COVID-19 patients","volume":"23","author":"Garg","year":"2021","journal-title":"Eur. J. Heart Fail."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1469","DOI":"10.2147\/IJGM.S295939","article-title":"Circulating miR-155 and JAK2\/STAT3 Axis in Acute Ischemic Stroke Patients and Its Relation to Post-Ischemic Inflammation and Associated Ischemic Stroke Risk Factors","volume":"14","year":"2021","journal-title":"Int. J. Gen. Med."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"2290","DOI":"10.1002\/jnr.24696","article-title":"Plasma endothelial microvesicles and their carrying miRNA-155 serve as biomarkers for ischemic stroke","volume":"98","author":"Zhang","year":"2020","journal-title":"J. Neurosci. Res."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Azouz, F., Arora, K., Krause, K., Nerurkar, V.R., and Kumar, M. (2019). Integrated MicroRNA and mRNA Profiling in Zika Virus-Infected Neurons. Viruses, 11.","DOI":"10.3390\/v11020162"},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Majer, A., Caligiuri, K.A., Gale, K.K., Niu, Y., Phillipson, C.S., Booth, T.F., and Booth, S.A. (2017). Correction: Induction of Multiple miR-200\/182 Members in the Brains of Mice Are Associated with Acute Herpes Simplex Virus 1 Encephalitis. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0172815"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"842288","DOI":"10.3389\/fnmol.2022.842288","article-title":"MicroRNA-21 Is a Versatile Regulator and Potential Treatment Target in Central Nervous System Disorders","volume":"15","author":"Bai","year":"2022","journal-title":"Front. Mol. Neurosci."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"3328","DOI":"10.1038\/s41598-017-02969-6","article-title":"Cerebrospinal fluid microRNAs are potential biomarkers of temporal lobe epilepsy and status epilepticus","volume":"7","author":"Raoof","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"971","DOI":"10.3892\/mmr.2014.2245","article-title":"Identification of miRNA-21 and miRNA-24 in plasma as potential early stage markers of acute cerebral infarction","volume":"10","author":"Zhou","year":"2014","journal-title":"Mol. Med. Rep."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"5243","DOI":"10.1182\/blood-2010-10-316083","article-title":"HHV-6 reactivation and its effect on delirium and cognitive functioning in hematopoietic cell transplantation recipients","volume":"117","author":"Zerr","year":"2011","journal-title":"Blood"},{"key":"ref_53","first-page":"e15331","article-title":"A Rare Case of Human Herpesvirus 6 Meningitis in an Immunocompetent Asian Male Presented with a Severe Intractable Headache","volume":"13","author":"Patel","year":"2021","journal-title":"Cureus"},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Slota, J.A., and Booth, S.A. (2019). MicroRNAs in Neuroinflammation: Implications in Disease Pathogenesis, Biomarker Discovery and Therapeutic Applications. Non-Coding RNA, 5.","DOI":"10.3390\/ncrna5020035"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"90","DOI":"10.3389\/fnmol.2020.00090","article-title":"MicroRNA-146a Is a Wide-Reaching Neuroinflammatory Regulator and Potential Treatment Target in Neurological Diseases","volume":"13","author":"Fan","year":"2020","journal-title":"Front. Mol. Neurosci."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"1500","DOI":"10.1097\/WNR.0b013e3283329c05","article-title":"HSV-1 infection of human brain cells induces miRNA-146a and Alzheimer-type inflammatory signaling","volume":"20","author":"Hill","year":"2009","journal-title":"Neuroreport"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1007\/s12031-015-0489-6","article-title":"The Expression and Significance of the Plasma Let-7 Family in Anti-N-methyl-D-aspartate Receptor Encephalitis","volume":"56","author":"Zhang","year":"2015","journal-title":"J. Mol. Neurosci."},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Long, G., Wang, F., Li, H., Yin, Z., Sandip, C., Lou, Y., Wang, Y., Chen, C., and Wang, D.W. (2013). Circulating miR-30a, miR-126 and let-7b as biomarker for ischemic stroke in humans. BMC Neurol., 13.","DOI":"10.1186\/1471-2377-13-178"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"796","DOI":"10.1111\/dmcn.13448","article-title":"Herpes simplex virus-induced anti-N-methyl-d-aspartate receptor encephalitis: A systematic literature review with analysis of 43 cases","volume":"59","author":"Nosadini","year":"2017","journal-title":"Dev. Med. Child Neurol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"ofac229","DOI":"10.1093\/ofid\/ofac229","article-title":"Human Herpesvirus-6 Detection in Cerebrospinal Fluid on the BioFire FilmArray Meningitis\/Encephalitis Panel in a High Human Immunodeficiency Virus-Prevalence African Setting","volume":"9","author":"Milburn","year":"2022","journal-title":"Open Forum Infect. Dis."},{"key":"ref_61","first-page":"1666","article-title":"miR-224\/miR-141 ratio as a novel diagnostic biomarker in renal cell carcinoma","volume":"16","author":"Chen","year":"2018","journal-title":"Oncol. Lett."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"125","DOI":"10.3389\/fnmol.2019.00125","article-title":"MicroRNAs in Microglia: How do MicroRNAs Affect Activation, Inflammation, Polarization of Microglia and Mediate the Interaction Between Microglia and Glioma?","volume":"12","author":"Guo","year":"2019","journal-title":"Front. Mol. Neurosci."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"6286","DOI":"10.1093\/hmg\/ddu348","article-title":"Early miR-155 upregulation contributes to neuroinflammation in Alzheimer\u2019s disease triple transgenic mouse model","volume":"23","author":"Guedes","year":"2014","journal-title":"Hum. Mol. Genet."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"1101","DOI":"10.1038\/nprot.2008.73","article-title":"Analyzing real-time PCR data by the comparative C(T) method","volume":"3","author":"Schmittgen","year":"2008","journal-title":"Nat. Protoc."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/13\/11201\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T20:07:56Z","timestamp":1760126876000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/13\/11201"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,7,7]]},"references-count":65,"journal-issue":{"issue":"13","published-online":{"date-parts":[[2023,7]]}},"alternative-id":["ijms241311201"],"URL":"https:\/\/doi.org\/10.3390\/ijms241311201","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,7,7]]}}}