{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,28]],"date-time":"2025-12-28T06:18:37Z","timestamp":1766902717996,"version":"build-2065373602"},"reference-count":54,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2023,7,7]],"date-time":"2023-07-07T00:00:00Z","timestamp":1688688000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31772817","2018BBF33001"],"award-info":[{"award-number":["31772817","2018BBF33001"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100016692","name":"Key R&amp;D Program of Ningxia Hui Autonomous Region","doi-asserted-by":"publisher","award":["31772817","2018BBF33001"],"award-info":[{"award-number":["31772817","2018BBF33001"]}],"id":[{"id":"10.13039\/100016692","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Endometritis in high-yield dairy cows adversely affects lactation length, milk quality, and the economics of dairy products. Endoplasmic reticulum stress (ERS) in bovine endometrial epithelial cells (BEECs) occurs as a consequence of diverse post-natal stressors, and plays a key role in a variety of inflammatory diseases. Nuclear-factor-erythroid-2-related factor 2 (Nrf2) is an important protective regulatory factor in numerous inflammatory responses. However, the mechanism by which Nrf2 modulates inflammation by participating in ERS remains unclear. The objective of the present study was to explore the role of Nrf2 in lipopolysaccharide (LPS)-induced injury to BEECs and to decipher the underlying molecular mechanisms of this injury. The expression of Nrf2- and ERS-related genes increased significantly in bovine uteri with endometritis. Isolated BEECs were treated with LPS to stimulate the inflammatory response. The expression of Nrf2 was significantly higher in cells exposed to LPS, which also induced ERS in BEECs. Activation of Nrf2 led to enhanced expression of the genes for the inflammation markers TNF-\u03b1, p65, IL-6, and IL-8 in BEECs. Moreover, stimulation of Nrf2 was accompanied by activation of ERS. In contrast, Nrf2 knockdown reduced the expression of TNF-\u03b1, p65, IL-6, and IL-8. Additionally, Nrf2 knockdown decreased expression of ERS-related genes for the GRP78, PERK, eIF2\u03b1, ATF4, and CHOP proteins. Collectively, our findings demonstrate that Nrf2 and ERS are activated during inflammation in BEECs. Furthermore, Nrf2 promotes the inflammatory response by activating the PERK pathway in ERS and inducing apoptosis in BEECs.<\/jats:p>","DOI":"10.3390\/ijms241311221","type":"journal-article","created":{"date-parts":[[2023,7,10]],"date-time":"2023-07-10T00:35:21Z","timestamp":1688949321000},"page":"11221","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Transcription Factor Nrf2 Modulates Lipopolysaccharide-Induced Injury in Bovine Endometrial Epithelial Cells"],"prefix":"10.3390","volume":"24","author":[{"given":"Pengjie","family":"Song","sequence":"first","affiliation":[{"name":"Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, College of Veterinary Medicine, Northwest A&F University, Xianyang 712100, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chen","family":"Liu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, College of Veterinary Medicine, Northwest A&F University, Xianyang 712100, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mingkun","family":"Sun","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, College of Veterinary Medicine, Northwest A&F University, Xianyang 712100, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianguo","family":"Liu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, College of Veterinary Medicine, Northwest A&F University, Xianyang 712100, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6815-4418","authenticated-orcid":false,"given":"Pengfei","family":"Lin","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, College of Veterinary Medicine, Northwest A&F University, Xianyang 712100, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huatao","family":"Chen","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, College of Veterinary Medicine, Northwest A&F University, Xianyang 712100, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dong","family":"Zhou","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, College of Veterinary Medicine, Northwest A&F University, Xianyang 712100, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keqiong","family":"Tang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, College of Veterinary Medicine, Northwest A&F University, Xianyang 712100, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aihua","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, College of Veterinary Medicine, Northwest A&F University, Xianyang 712100, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yaping","family":"Jin","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, College of Veterinary Medicine, Northwest A&F University, Xianyang 712100, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,7,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1516","DOI":"10.1016\/j.theriogenology.2005.08.021","article-title":"Defining postpartum uterine disease in cattle","volume":"65","author":"Sheldon","year":"2006","journal-title":"Theriogenology"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1111\/j.1439-0531.2009.01485.x","article-title":"Clinical and subclinical endometritis in the mare: Both threats to fertility","volume":"44","author":"LeBlanc","year":"2009","journal-title":"Reprod. Domest. Anim. Zuchthyg."},{"key":"ref_3","first-page":"245","article-title":"Subclinical endometritis in dairy cattle and its effect on fertility\u2014A review of recent publications","volume":"120","author":"Lincke","year":"2007","journal-title":"Berl. Munch. Tierarztl. Wochenschr."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"614054","DOI":"10.3389\/fvets.2020.614054","article-title":"MicroRNAome: Potential and Veritable Immunomolecular Therapeutic and Diagnostic Baseline for Lingering Bovine Endometritis","volume":"7","author":"Oladejo","year":"2020","journal-title":"Front. Vet. Sci."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Saito, A., and Imaizumi, K. (2018). Unfolded Protein Response-Dependent Communication and Contact among Endoplasmic Reticulum, Mitochondria, and Plasma Membrane. Int. J. Mol. Sci., 19.","DOI":"10.20944\/preprints201810.0023.v1"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/j.tibs.2015.01.002","article-title":"UPR, autophagy, and mitochondria crosstalk underlies the ER stress response","volume":"40","author":"Senft","year":"2015","journal-title":"Trends Biochem. Sci."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"101195","DOI":"10.1016\/j.redox.2019.101195","article-title":"Inhibition of Brd4 alleviates renal ischemia\/reperfusion injury-induced apoptosis and endoplasmic reticulum stress by blocking FoxO4-mediated oxidative stress","volume":"24","author":"Liu","year":"2019","journal-title":"Redox Biol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"864426","DOI":"10.3389\/fncel.2022.864426","article-title":"Endoplasmic Reticulum Stress and the Unfolded Protein Response in Cerebral Ischemia\/Reperfusion Injury","volume":"16","author":"Wang","year":"2022","journal-title":"Front. Cell. Neurosci."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"6129","DOI":"10.1007\/s00253-020-10614-y","article-title":"Crosstalk between ER stress, NLRP3 inflammasome, and inflammation","volume":"104","author":"Li","year":"2020","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Liu, J., Qiu, R., Liu, R., Song, P., Lin, P., Chen, H., Zhou, D., Wang, A., and Jin, Y. (2022). Autophagy Mediates Escherichia Coli-Induced Cellular Inflammatory Injury by Regulating Calcium Mobilization, Mitochondrial Dysfunction, and Endoplasmic Reticulum Stress. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms232214174"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"6655417","DOI":"10.1155\/2021\/6655417","article-title":"Unraveling the Molecular Nexus between GPCRs, ERS, and EMT","volume":"2021","author":"Kumari","year":"2021","journal-title":"Mediat. Inflamm."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1038\/s41569-021-00511-w","article-title":"Endoplasmic reticulum stress and unfolded protein response in cardiovascular diseases","volume":"18","author":"Ren","year":"2021","journal-title":"Nat. Rev. Cardiol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1051","DOI":"10.1038\/s41419-022-05444-x","article-title":"Endoplasmic reticulum stress-mediated cell death in liver injury","volume":"13","author":"Zhang","year":"2022","journal-title":"Cell Death Dis."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"602307","DOI":"10.3389\/fphar.2020.602307","article-title":"Melatonin Inhibits Glucose-Induced Apoptosis in Osteoblastic Cell Line Through PERK-eIF2\u03b1-ATF4 Pathway","volume":"11","author":"Zhou","year":"2020","journal-title":"Front. Pharmacol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"190","DOI":"10.3389\/fncel.2019.00190","article-title":"Endoplasmic Reticulum Stress Is Involved in Stress-Induced Hypothalamic Neuronal Injury in Rats via the PERK-ATF4-CHOP and IRE1-ASK1-JNK Pathways","volume":"13","author":"Yi","year":"2019","journal-title":"Front. Cell. Neurosci."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"He, F., Ru, X., and Wen, T. (2020). NRF2, a Transcription Factor for Stress Response and Beyond. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21134777"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1016\/j.bbadis.2016.11.005","article-title":"Nrf2 signaling pathway: Pivotal roles in inflammation","volume":"1863","author":"Ahmed","year":"2017","journal-title":"Biochim. Biophys. Acta. Mol. Basis Dis."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1146\/annurev-pharmtox-011112-140320","article-title":"Role of nrf2 in oxidative stress and toxicity","volume":"53","author":"Ma","year":"2013","journal-title":"Annu. Rev. Pharmacol. Toxicol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"111231","DOI":"10.1016\/j.ecoenv.2020.111231","article-title":"Lead exposure activates the Nrf2\/Keap1 pathway, aggravates oxidative stress, and induces reproductive damage in female mice","volume":"207","author":"Jiang","year":"2021","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"163","DOI":"10.3389\/fmolb.2020.00163","article-title":"Melatonin Inhibits Oxidative Stress and Apoptosis in Cryopreserved Ovarian Tissues via Nrf2\/HO-1 Signaling Pathway","volume":"7","author":"Sun","year":"2020","journal-title":"Front. Mol. Biosci."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1016\/j.mam.2011.10.006","article-title":"Oxidative stress in health and disease: The therapeutic potential of Nrf2 activation","volume":"32","author":"Hybertson","year":"2011","journal-title":"Mol. Asp. Med."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1080\/08923973.2021.2012483","article-title":"Nrf2\/HO-1 pathway is involved the anti-inflammatory action of intrauterine infusion of platelet-rich plasma against lipopolysaccharides in endometritis","volume":"44","author":"Zhang","year":"2022","journal-title":"Immunopharmacol. Immunotoxicol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.rvsc.2020.11.022","article-title":"Andrographolide attenuates inflammatory response induced by LPS via activating Nrf2 signaling pathway in bovine endometrial epithelial cells","volume":"134","author":"Fu","year":"2021","journal-title":"Res. Vet. Sci."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/j.theriogenology.2021.09.001","article-title":"Increasing of matrix metalloproteinase 3 in bovine endometritis","volume":"175","author":"Zhang","year":"2021","journal-title":"Theriogenology"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1016\/j.theriogenology.2020.05.015","article-title":"Decreasing of S100A4 in bovine endometritis in vivo and in vitro","volume":"153","author":"Li","year":"2020","journal-title":"Theriogenology"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1140","DOI":"10.1007\/s12011-021-02731-0","article-title":"The Antioxidant Role of Selenium via GPx1 and GPx4 in LPS-Induced Oxidative Stress in Bovine Endometrial Cells","volume":"200","author":"Adeniran","year":"2022","journal-title":"Biol. Trace Elem. Res."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1186\/s11658-021-00294-5","article-title":"TBHQ attenuates ferroptosis against 5-fluorouracil-induced intestinal epithelial cell injury and intestinal mucositis via activation of Nrf2","volume":"26","author":"Deng","year":"2021","journal-title":"Cell. Mol. Biol. Lett."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1007\/s00018-015-2052-6","article-title":"The endoplasmic reticulum: Structure, function and response to cellular signaling","volume":"73","author":"Schwarz","year":"2016","journal-title":"Cell. Mol. Life Sci. CMLS"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"109435","DOI":"10.1016\/j.intimp.2022.109435","article-title":"Protective effects of chicoric acid on LPS-induced endometritis in mice via inhibiting ferroptosis by Nrf2\/HO-1 signal axis","volume":"113","author":"Wang","year":"2022","journal-title":"Int. Immunopharmacol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"108729","DOI":"10.1016\/j.fsi.2023.108729","article-title":"The role of Nrf2 signaling pathway in the mud crab (Scylla paramamosain) in response to Vibrio parahaemolyticus infection","volume":"136","author":"Cheng","year":"2023","journal-title":"Fish Shellfish Immunol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"133","DOI":"10.14348\/molcells.2023.0028","article-title":"Molecular Basis of the KEAP1-NRF2 Signaling Pathway","volume":"46","author":"Suzuki","year":"2023","journal-title":"Mol. Cells"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"967193","DOI":"10.3389\/fimmu.2022.967193","article-title":"Nrf2-mediated anti-inflammatory polarization of macrophages as therapeutic targets for osteoarthritis","volume":"13","author":"Wang","year":"2022","journal-title":"Front. Immunol."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Yasuo, T., and Kitaya, K. (2022). Challenges in Clinical Diagnosis and Management of Chronic Endometritis. Diagnostics, 12.","DOI":"10.3390\/diagnostics12112711"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"344","DOI":"10.1016\/j.fertnstert.2018.04.012","article-title":"Endometritis: New time, new concepts","volume":"110","author":"Kitaya","year":"2018","journal-title":"Fertil. Steril."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"103192","DOI":"10.1016\/j.jri.2020.103192","article-title":"Inhibition of TLR2\/TLR4 alleviates the Neisseria gonorrhoeae infection damage in human endometrial epithelial cells via Nrf2 and NF-K\u03b2signaling","volume":"142","author":"Yang","year":"2020","journal-title":"J. Reprod. Immunol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/j.micpath.2018.06.031","article-title":"Protective effects of apigenin on LPS-induced endometritis via activating Nrf2 signaling pathway","volume":"123","author":"Jiang","year":"2018","journal-title":"Microb. Pathog."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"107738","DOI":"10.1016\/j.intimp.2021.107738","article-title":"Berberine attenuates the inflammatory response by activating the Keap1\/Nrf2 signaling pathway in bovine endometrial epithelial cells","volume":"96","author":"Fu","year":"2021","journal-title":"Int. Immunopharmacol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"972","DOI":"10.1111\/rda.13939","article-title":"Aucubin ameliorates the LPS-induced inflammatory response in bovine endometrial epithelial cells by inhibiting NF-\u03baB and activating the Keap1\/Nrf2 signalling pathway","volume":"56","author":"Gao","year":"2021","journal-title":"Reprod. Domest. Anim. Zuchthyg."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Gugliandolo, E., Fusco, R., Licata, P., Peritore, A.F., D\u2019Amico, R., Cordaro, M., Siracusa, R., Cuzzocrea, S., and Crupi, R. (2020). Protective Effect of Hydroxytyrosol on LPS-Induced Inflammation and Oxidative Stress in Bovine Endometrial Epithelial Cell Line. Vet. Sci., 7.","DOI":"10.3390\/vetsci7040161"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"109822","DOI":"10.1016\/j.intimp.2023.109822","article-title":"Meloxicam inhibited oxidative stress and inflammatory response of LPS-stimulated bovine endometrial epithelial cells through Nrf2 and NF-\u03baB pathways","volume":"116","author":"Cui","year":"2023","journal-title":"Int. Immunopharmacol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1038\/s41573-021-00320-3","article-title":"Pharmacological targeting of endoplasmic reticulum stress in disease","volume":"21","author":"Marciniak","year":"2022","journal-title":"Nat. Rev. Drug Discov."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"2825","DOI":"10.3389\/fimmu.2019.02825","article-title":"ER Stress and the UPR in Shaping Intestinal Tissue Homeostasis and Immunity","volume":"10","author":"Coleman","year":"2019","journal-title":"Front. Immunol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1477","DOI":"10.1016\/j.molcel.2022.03.025","article-title":"Reshaping endoplasmic reticulum quality control through the unfolded protein response","volume":"82","author":"Wiseman","year":"2022","journal-title":"Mol. Cell"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1186\/s12974-018-1077-9","article-title":"IRE1\u03b1 inhibition decreased TXNIP\/NLRP3 inflammasome activation through miR-17-5p after neonatal hypoxic-ischemic brain injury in rats","volume":"15","author":"Chen","year":"2018","journal-title":"J. Neuroinflamm."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1186\/s12974-020-01796-3","article-title":"IRE1\u03b1 inhibition attenuates neuronal pyroptosis via miR-125\/NLRP1 pathway in a neonatal hypoxic-ischemic encephalopathy rat model","volume":"17","author":"Huang","year":"2020","journal-title":"J. Neuroinflamm."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/j.intimp.2018.08.028","article-title":"Melatonin inhibits endoplasmic reticulum stress-associated TXNIP\/NLRP3 inflammasome activation in lipopolysaccharide-induced endometritis in mice","volume":"64","author":"Hu","year":"2018","journal-title":"Int. Immunopharmacol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1087","DOI":"10.1002\/jcp.30604","article-title":"Hyperthermia alters interleukin-6 production in response to lipopolysaccharide via endoplasmic reticulum stress in bovine endometrial cells","volume":"237","author":"Sakai","year":"2022","journal-title":"J. Cell. Physiol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"3083","DOI":"10.3389\/fimmu.2018.03083","article-title":"The C\/EBP Homologous Protein (CHOP) Transcription Factor Functions in Endoplasmic Reticulum Stress-Induced Apoptosis and Microbial Infection","volume":"9","author":"Hu","year":"2018","journal-title":"Front. Immunol."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Asmat, T.M., Tenenbaum, T., Jonsson, A.B., Schwerk, C., and Schroten, H. (2014). Impact of calcium signaling during infection of Neisseria meningitidis to human brain microvascular endothelial cells. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0114474"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"729094","DOI":"10.3389\/fimmu.2021.729094","article-title":"Inhibition of IP3R\/Ca2+ Dysregulation Protects Mice From Ventilator-Induced Lung Injury via Endoplasmic Reticulum and Mitochondrial Pathways","volume":"12","author":"Ye","year":"2021","journal-title":"Front. Immunol."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Szyma\u0144ski, J., Janikiewicz, J., Michalska, B., Patalas-Krawczyk, P., Perrone, M., Zi\u00f3\u0142kowski, W., Duszy\u0144ski, J., Pinton, P., Dobrzy\u0144, A., and Wi\u0119ckowski, M.R. (2017). Interaction of Mitochondria with the Endoplasmic Reticulum and Plasma Membrane in Calcium Homeostasis, Lipid Trafficking and Mitochondrial Structure. Int. J. Mol. Sci., 18.","DOI":"10.3390\/ijms18071576"},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Xue, Y., Zhou, S., Xie, W., Meng, M., Ma, N., Zhang, H., Wang, Y., Chang, G., and Shen, X. (2022). STIM1-Orai1 Interaction Exacerbates LPS-Induced Inflammation and Endoplasmic Reticulum Stress in Bovine Hepatocytes through Store-Operated Calcium Entry. Genes, 13.","DOI":"10.3390\/genes13050874"},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Zeeshan, H.M., Lee, G.H., Kim, H.R., and Chae, H.J. (2016). Endoplasmic Reticulum Stress and Associated ROS. Int. J. Mol. Sci., 17.","DOI":"10.3390\/ijms17030327"},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Song, P., Liu, C., Sun, M., Liu, J., Lin, P., Wang, A., and Jin, Y. (2022). Oxidativae Stress Induces Bovine Endometrial Epithelial Cell Damage through Mitochondria-Dependent Pathways. Animals, 12.","DOI":"10.3390\/ani12182444"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/13\/11221\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T20:08:18Z","timestamp":1760126898000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/13\/11221"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,7,7]]},"references-count":54,"journal-issue":{"issue":"13","published-online":{"date-parts":[[2023,7]]}},"alternative-id":["ijms241311221"],"URL":"https:\/\/doi.org\/10.3390\/ijms241311221","relation":{},"ISSN":["1422-0067"],"issn-type":[{"type":"electronic","value":"1422-0067"}],"subject":[],"published":{"date-parts":[[2023,7,7]]}}}