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It is extremely important to develop high-quality artificial corneas because there are not enough donor corneas accessible for cornea transplantation. One of the most-often utilized materials is collagen, which is the primary component of natural cornea. Collagen-based corneal repair materials have good physicochemical properties and excellent biocompatibility, but how to promote the regeneration of the corneal nerve after keratoplasty is still a big challenge. In this research, in order to promote the growth of nerve cells on a collagen (Col) substrate, a novel collagen-based material was synthesized starting from the functionalization of collagen with unsaturated methacryloyl groups that three-dimensionally photopolymerize to a 3D network of chemically crosslinked collagen (ColMA), onto which taurine molecules were eventually grafted (ColMA-Tr). The physicochemical properties and biocompatibility of the Col, ColMA and ColMA-Tr films were evaluated. By analyzing the results, we found that all the three samples had good moisture retention and aq high covalent attachment of methacryloyl groups followed by their photopolymerization improved the mechanical properties of the ColMA and ColMA-Tr. Most importantly, compared with ColMA, the taurine-modified collagen-MA film significantly promoted the growth of nerve cells and corneal epithelial cells on its surface. Our preliminary results suggest that this novel ColMA-Tr film may have potential use in cornea tissue engineering in the future.<\/jats:p>","DOI":"10.3390\/ijms241411248","type":"journal-article","created":{"date-parts":[[2023,7,10]],"date-time":"2023-07-10T00:35:21Z","timestamp":1688949321000},"page":"11248","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Preparation and Characterization of a Photo-Crosslinked Methacryloyl-Collagen Composite Film to Promote Corneal Nerve Regeneration via Surface Grafting of Taurine Molecules"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9445-0859","authenticated-orcid":false,"given":"Yang","family":"Liu","sequence":"first","affiliation":[{"name":"Institute of Biomedical Engineering and Health Sciences, Changzhou University, Changzhou 213164, China"},{"name":"School of Pharmacy, School of Biology and Food Engineering, Changzhou University, Changzhou 213164, China"},{"name":"School of Medical and Health Engineering, Changzhou University, Changzhou 213164, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuanlei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Engineering and Health Sciences, Changzhou University, Changzhou 213164, China"},{"name":"School of Pharmacy, School of Biology and Food Engineering, Changzhou University, Changzhou 213164, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanhui","family":"Kong","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Engineering and Health Sciences, Changzhou University, Changzhou 213164, China"},{"name":"School of Pharmacy, School of Biology and Food Engineering, Changzhou University, Changzhou 213164, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huiyu","family":"Liu","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Engineering and Health Sciences, Changzhou University, Changzhou 213164, China"},{"name":"School of Pharmacy, School of Biology and Food Engineering, Changzhou University, Changzhou 213164, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng","family":"Chen","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Engineering and Health Sciences, Changzhou University, Changzhou 213164, China"},{"name":"School of Pharmacy, School of Biology and Food Engineering, Changzhou University, Changzhou 213164, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenyu","family":"Gao","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Engineering and Health Sciences, Changzhou University, Changzhou 213164, China"},{"name":"School of Pharmacy, School of Biology and Food Engineering, Changzhou University, Changzhou 213164, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaowei","family":"Xi","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Engineering and Health Sciences, Changzhou University, Changzhou 213164, China"},{"name":"School of Pharmacy, School of Biology and Food Engineering, Changzhou University, Changzhou 213164, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8691-5110","authenticated-orcid":false,"given":"Hui","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Medical Information Engineering, Gannan Medical University, Ganzhou 341000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1970-8239","authenticated-orcid":false,"given":"Linhong","family":"Deng","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Engineering and Health Sciences, Changzhou University, Changzhou 213164, China"},{"name":"School of Medical and Health Engineering, Changzhou University, Changzhou 213164, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,7,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1701434","DOI":"10.1002\/adhm.201701434","article-title":"Natural Biomaterials for Corneal Tissue Engineering, Repair, and Regeneration","volume":"7","author":"Palchesko","year":"2018","journal-title":"Adv. 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