{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T08:55:52Z","timestamp":1790844952091,"version":"4.1.0"},"reference-count":107,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2023,7,13]],"date-time":"2023-07-13T00:00:00Z","timestamp":1689206400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000104","name":"NASA","doi-asserted-by":"publisher","award":["80NSSC18K1480"],"award-info":[{"award-number":["80NSSC18K1480"]}],"id":[{"id":"10.13039\/100000104","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>An organoid is a 3D organization of cells that can recapitulate some of the structure and function of native tissue. Recent work has seen organoids gain prominence as a valuable model for studying tissue development, drug discovery, and potential clinical applications. The requirements for the successful culture of organoids in vitro differ significantly from those of traditional monolayer cell cultures. The generation and maturation of high-fidelity organoids entails developing and optimizing environmental conditions to provide the optimal cues for growth and 3D maturation, such as oxygenation, mechanical and fluidic activation, nutrition gradients, etc. To this end, we discuss the four main categories of bioreactors used for organoid culture: stirred bioreactors (SBR), microfluidic bioreactors (MFB), rotating wall vessels (RWV), and electrically stimulating (ES) bioreactors. We aim to lay out the state-of-the-art of both commercial and in-house developed bioreactor systems, their benefits to the culture of organoids derived from various cells and tissues, and the limitations of bioreactor technology, including sterilization, accessibility, and suitability and ease of use for long-term culture. Finally, we discuss future directions for improvements to existing bioreactor technology and how they may be used to enhance organoid culture for specific applications.<\/jats:p>","DOI":"10.3390\/ijms241411427","type":"journal-article","created":{"date-parts":[[2023,7,14]],"date-time":"2023-07-14T00:28:06Z","timestamp":1689294486000},"page":"11427","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":101,"title":["Bioreactor Technologies for Enhanced Organoid Culture"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8749-5952","authenticated-orcid":false,"given":"Joseph P.","family":"Licata","sequence":"first","affiliation":[{"name":"Department of Bioengineering, College of Engineering, Temple University, Philadelphia, PA 19122, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyle H.","family":"Schwab","sequence":"additional","affiliation":[{"name":"Department of Bioengineering, College of Engineering, Temple University, Philadelphia, PA 19122, USA"},{"name":"Neurobiology, Neurodegeneration & Repair Laboratory, National Eye Institute, National Institutes of Health, Bethesda, MD 20892, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yah-el","family":"Har-el","sequence":"additional","affiliation":[{"name":"Department of Bioengineering, College of Engineering, Temple University, Philadelphia, PA 19122, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan A.","family":"Gerstenhaber","sequence":"additional","affiliation":[{"name":"Department of Bioengineering, College of Engineering, Temple University, Philadelphia, PA 19122, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4954-3498","authenticated-orcid":false,"given":"Peter I.","family":"Lelkes","sequence":"additional","affiliation":[{"name":"Department of Bioengineering, College of Engineering, Temple University, Philadelphia, PA 19122, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,7,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1038\/ncb3312","article-title":"Organoids as an In Vitro Model of Human Development and Disease","volume":"18","author":"Fatehullah","year":"2016","journal-title":"Nat. Cell Biol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"100345","DOI":"10.1016\/j.mtbio.2022.100345","article-title":"Bioengineered Co-Culture of Organoids to Recapitulate Host-Microbe Interactions","volume":"16","author":"Kim","year":"2022","journal-title":"Mater. Today BIO"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1038\/s41580-020-0259-3","article-title":"Human Organoids: Model Systems for Human Biology and Medicine","volume":"21","author":"Kim","year":"2020","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"C151","DOI":"10.1152\/ajpcell.00120.2020","article-title":"A Brief History of Organoids","volume":"319","author":"Novellasdemunt","year":"2020","journal-title":"Am. J. Physiol. Cell Physiol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1111","DOI":"10.1016\/j.biotechadv.2018.03.011","article-title":"Stem Cells: Their Source, Potency and Use in Regenerative Therapies with Focus on Adipose-Derived Stem Cells\u2014A Review","volume":"36","author":"Bacakova","year":"2018","journal-title":"Biotechnol. Adv."},{"key":"ref_6","unstructured":"Lin, Y., and Chen, G. (2008). StemBook, Harvard Stem Cell Institute."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1186\/s13287-019-1455-y","article-title":"Research and Therapy with Induced Pluripotent Stem Cells (IPSCs): Social, Legal, and Ethical Considerations","volume":"10","author":"Moradi","year":"2019","journal-title":"Stem Cell Res. Ther."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Oldak, B., Wildschutz, E., Bondarenko, V., Aguilera-Castrejon, A., Zhao, C., Tarazi, S., Comar, M.-Y., Ashouokhi, S., Lokshtanov, D., and Roncato, F. (2023). Transgene-Free Ex Utero Derivation of a Human Post-Implantation Embryo Model Solely from Genetically Unmodified Na\u00efve PSCs. bioRxiv.","DOI":"10.1101\/2023.06.14.544922"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"15112","DOI":"10.1038\/ncomms15112","article-title":"An Extra-Uterine System to Physiologically Support the Extreme Premature Lamb","volume":"8","author":"Partridge","year":"2017","journal-title":"Nat. Commun."},{"key":"ref_10","unstructured":"Erickson, L.E. (2019). Comprehensive Biotechnology, Elsevier."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Vandermies, M., and Fickers, P. (2019). Bioreactor-Scale Strategies for the Production of Recombinant Protein in the Yeast Yarrowia Lipolytica. Microorganisms, 7.","DOI":"10.3390\/microorganisms7020040"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"397","DOI":"10.2174\/138920105774370580","article-title":"The Bioreactor: A Powerful Tool for Large-Scale Culture of Animal Cells","volume":"6","author":"Wang","year":"2005","journal-title":"Curr. Pharm. Biotechnol."},{"key":"ref_13","first-page":"1","article-title":"Recent Advances in Bioreactors for Cell-Based Therapies","volume":"7","author":"Grayson","year":"2018","journal-title":"F1000Research"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1186\/s13287-020-02109-4","article-title":"Overcoming Bioprocess Bottlenecks in the Large-Scale Expansion of High-Quality HiPSC Aggregates in Vertical-Wheel Stirred Suspension Bioreactors","volume":"12","author":"Borys","year":"2021","journal-title":"Stem Cell Res. Ther."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"178","DOI":"10.3389\/fnut.2020.575146","article-title":"Scale-Up Technologies for the Manufacture of Adherent Cells","volume":"7","author":"Bellani","year":"2020","journal-title":"Front. Nutr."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"33","DOI":"10.3389\/fmolb.2020.00033","article-title":"Is It Time to Start Transitioning from 2D to 3D Cell Culture?","volume":"7","author":"Jensen","year":"2020","journal-title":"Front. Mol. Biosci."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1002\/biot.201200162","article-title":"Bioreactors in Tissue Engineering\u2014Principles, Applications and Commercial Constraints","volume":"8","author":"Hansmann","year":"2013","journal-title":"Biotechnol. J."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1002\/bit.10788","article-title":"Fluid Mechanics of a Spinner-Flask Bioreactor","volume":"85","author":"Sucosky","year":"2004","journal-title":"Biotechnol. Bioeng."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"163","DOI":"10.5661\/bger-26-163","article-title":"Supply of Nutrients to Cells in Engineered Tissues","volume":"26","author":"Rouwkema","year":"2009","journal-title":"Biotechnol. Genet. Eng. Rev."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Goto-Silva, L., Ayad, N.M.E., Herzog, I.L., Silva, N.P., Lamien, B., Orlande, H.R.B., da Costa Souza, A., Ribeiro, S., Martins, M., and Domont, G.B. (2019). Computational Fluid Dynamic Analysis of Physical Forces Playing a Role in Brain Organoid Cultures in Two Different Multiplex Platforms. BMC Dev. Biol., 19.","DOI":"10.1186\/s12861-019-0183-y"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1038\/nprot.2017.152","article-title":"Generation of Human Brain Region\u2013Specific Organoids Using a Miniaturized Spinning Bioreactor","volume":"13","author":"Qian","year":"2018","journal-title":"Nat. Protoc."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1186\/s13287-018-0907-0","article-title":"Use of Bioreactors for Culturing Human Retinal Organoids Improves Photoreceptor Yields","volume":"9","author":"West","year":"2018","journal-title":"Stem Cell Res. Ther."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1089\/ten.tec.2015.0375","article-title":"Analytic Models of Oxygen and Nutrient Diffusion, Metabolism Dynamics, and Architecture Optimization in Three-Dimensional Tissue Constructs with Applications and Insights in Cerebral Organoids","volume":"22","author":"McMurtrey","year":"2016","journal-title":"Tissue Eng. Part. C Methods"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"475","DOI":"10.2478\/s11658-006-0039-x","article-title":"A Comparative Gene-Expression Analysis of CD34+ Hematopoietic Stem and Progenitor Cells Grown in Static and Stirred Culture Systems","volume":"11","author":"Li","year":"2006","journal-title":"Cell. Mol. Biol. Lett."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"2329","DOI":"10.1038\/nprot.2014.158","article-title":"Generation of Cerebral Organoids from Human Pluripotent Stem Cells","volume":"9","author":"Lancaster","year":"2014","journal-title":"Nat. Protoc."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1038\/nature12517","article-title":"Cerebral Organoids Model Human Brain Development and Microcephaly","volume":"501","author":"Lancaster","year":"2013","journal-title":"Nature"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1238","DOI":"10.1016\/j.cell.2016.04.032","article-title":"Brain-Region-Specific Organoids Using Mini-Bioreactors for Modeling ZIKV Exposure","volume":"165","author":"Qian","year":"2016","journal-title":"Cell"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1038\/nmeth.1939","article-title":"Derivation, Expansion and Differentiation of Induced Pluripotent Stem Cells in Continuous Suspension Cultures","volume":"9","author":"Fluri","year":"2012","journal-title":"Nat. Methods"},{"key":"ref_29","unstructured":"Chisti, Y. (2006). Basic Biotechnology, Cambridge University Press. [3rd]."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1016\/j.jbiotec.2007.01.006","article-title":"Embryonic Stem Cells Remain Highly Pluripotent Following Long Term Expansion as Aggregates in Suspension Bioreactors","volume":"129","author":"Cormier","year":"2007","journal-title":"J. Biotechnol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1002\/hep.31037","article-title":"Large-Scale Production of LGR5-Positive Bipotential Human Liver Stem Cells","volume":"72","author":"Schneeberger","year":"2020","journal-title":"Hepatology"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"e00084","DOI":"10.1016\/j.ohx.2019.e00084","article-title":"Spin\u221e: An Updated Miniaturized Spinning Bioreactor Design for the Generation of Human Cerebral Organoids from Pluripotent Stem Cells","volume":"6","author":"Balotin","year":"2019","journal-title":"HardwareX"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1036","DOI":"10.1039\/b909900j","article-title":"Fundamentals of Microfluidic Cell Culture in Controlled Microenvironments","volume":"39","author":"Craighead","year":"2010","journal-title":"Chem. Soc. Rev."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1146\/annurev.fluid.36.050802.122124","article-title":"Engineering Flows in Small Devices: Microfluidics toward a Lab-on-a-Chip","volume":"36","author":"Stone","year":"2004","journal-title":"Annu. Rev. Fluid. Mech."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"5821","DOI":"10.1021\/ac9007573","article-title":"Electrochemical Detection for Paper-Based Microfluidics","volume":"81","author":"Dungchai","year":"2009","journal-title":"Anal. Chem."},{"key":"ref_36","first-page":"E2293","article-title":"Multisensor-Integrated Organs-on-Chips Platform for Automated and Continual in Situ Monitoring of Organoid Behaviors","volume":"114","author":"Zhang","year":"2017","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1238","DOI":"10.1039\/b707401h","article-title":"Micro- and Nanomechanical Sensors for Environmental, Chemical, and Biological Detection","volume":"7","author":"Waggoner","year":"2007","journal-title":"Lab Chip"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"760","DOI":"10.1038\/nbt.2989","article-title":"Microfluidic Organs-on-Chips","volume":"32","author":"Bhatia","year":"2014","journal-title":"Nat. Biotechnol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"2165","DOI":"10.1039\/c2lc40074j","article-title":"Human Gut-on-a-Chip Inhabited by Microbial Flora That Experiences Intestinal Peristalsis-like Motions and Flow","volume":"12","author":"Kim","year":"2012","journal-title":"Lab Chip"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/j.pharmthera.2017.10.018","article-title":"Emerging Tumor Spheroids Technologies for 3D in Vitro Cancer Modeling","volume":"184","author":"Rodrigues","year":"2018","journal-title":"Pharmacol. Ther."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1007\/s13206-019-3402-5","article-title":"Hydrogel-Integrated Microfluidic Systems for Advanced Stem Cell Engineering","volume":"13","author":"An","year":"2019","journal-title":"BioChip J."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1039\/b614872g","article-title":"A Novel 3D Mammalian Cell Perfusion-Culture System in Microfluidic Channels","volume":"7","author":"Toh","year":"2007","journal-title":"Lab Chip"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"015009","DOI":"10.1088\/1758-5082\/6\/1\/015009","article-title":"A Microfluidic Chip with a U-Shaped Microstructure Array for Multicellular Spheroid Formation, Culturing and Analysis","volume":"6","author":"Fu","year":"2014","journal-title":"Biofabrication"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"3606","DOI":"10.1039\/C8LC00869H","article-title":"In Situ Differentiation and Generation of Functional Liver Organoids from Human IPSCs in a 3D Perfusable Chip System","volume":"18","author":"Wang","year":"2018","journal-title":"Lab Chip"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"e46188","DOI":"10.7554\/eLife.46188","article-title":"Merging Organoid and Organ-on-a-Chip Technology to Generate Complex Multi-Layer Tissue Models in a Human Retina-on-a-Chip Platform","volume":"8","author":"Achberger","year":"2019","journal-title":"eLife"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"2395","DOI":"10.1039\/C6LC01554A","article-title":"Organ-on-a-Chip Devices Advance to Market","volume":"17","author":"Zhang","year":"2017","journal-title":"Lab Chip"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1146\/annurev.matsci.28.1.153","article-title":"Soft Lithography","volume":"28","author":"Xia","year":"1998","journal-title":"Annu. Rev. Mater. Sci."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1016\/j.bios.2014.07.029","article-title":"Advantages and Challenges of Microfluidic Cell Culture in Polydimethylsiloxane Devices","volume":"63","author":"Halldorsson","year":"2015","journal-title":"Biosens. Bioelectron."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1509","DOI":"10.4155\/bio.12.133","article-title":"Microfluidic 3D Cell Culture: Potential Application for Tissue-Based Bioassays","volume":"4","author":"Li","year":"2012","journal-title":"Bioanalysis"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1484","DOI":"10.1039\/b612140c","article-title":"PDMS Absorption of Small Molecules and Consequences in Microfluidic Applications","volume":"6","author":"Toepke","year":"2006","journal-title":"Lab Chip"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"368","DOI":"10.1038\/nature05058","article-title":"The Origins and the Future of Microfluidics","volume":"442","author":"Whitesides","year":"2006","journal-title":"Nature"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1577","DOI":"10.3389\/fpls.2019.01577","article-title":"Comparison of Microgravity Analogs to Spaceflight in Studies of Plant Growth and Development","volume":"10","author":"Kiss","year":"2019","journal-title":"Front. Plant Sci."},{"key":"ref_53","unstructured":"Schwarz, R.P., and Wolf, D.A. (1991). Rotating Bio-Reactor Cell Culture Apparatus. (US4988623A), Available online: https:\/\/patents.google.com\/patent\/US4988623A\/en."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"F12","DOI":"10.1152\/ajprenal.2001.281.1.F12","article-title":"Optimized Suspension Culture: The Rotating-Wall Vessel","volume":"281","author":"Hammond","year":"2001","journal-title":"Am. J. Physiol. Ren. Physiol."},{"key":"ref_55","first-page":"3868","article-title":"Culturing and Applications of Rotating Wall Vessel Bioreactor Derived 3D Epithelial Cell Models","volume":"3","author":"Radtke","year":"2012","journal-title":"J. Vis. Exp."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1002\/jcb.240510309","article-title":"Reduced Shear Stress: A Major Component in the Ability of Mammalian Tissues to Form Three-Dimensional Assemblies in Simulated Microgravity","volume":"51","author":"Goodwin","year":"1993","journal-title":"J. Cell Biochem."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1016\/S1387-2656(08)00011-2","article-title":"The Application of Low Shear Modeled Microgravity to 3-D Cell Biology and Tissue Engineering","volume":"14","author":"Navran","year":"2008","journal-title":"Biotechnol. Annu. Rev."},{"key":"ref_58","unstructured":"Navran, S. (2007). Cell Technology for Cell Products, Springer."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1016\/j.jbiotec.2010.09.950","article-title":"Computational Fluid Dynamics Modeling of Momentum Transport in Rotating Wall Perfused Bioreactor for Cartilage Tissue Engineering","volume":"150","author":"Cinbiz","year":"2010","journal-title":"J. Biotechnol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/j.stem.2015.12.005","article-title":"Stem Cell Organoid Engineering","volume":"18","author":"Yin","year":"2016","journal-title":"Cell Stem Cell"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"2116","DOI":"10.1038\/nprot.2006.311","article-title":"Real-Time Assessment of Three-Dimensional Cell Aggregation in Rotating Wall Vessel Bioreactors In Vitro","volume":"1","author":"Botta","year":"2006","journal-title":"Nat. Protoc."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"159","DOI":"10.2217\/rme-2017-0103","article-title":"Cell Therapy-Processing Economics: Small-Scale Microfactories as a Stepping Stone toward Large-Scale Macrofactories","volume":"13","author":"Harrison","year":"2018","journal-title":"Regen. Med."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"1650","DOI":"10.1002\/sctm.21-0151","article-title":"A Scalable System for Generation of Mesenchymal Stem Cells Derived from Induced Pluripotent Cells Employing Bioreactors and Degradable Microcarriers","volume":"10","author":"Rogers","year":"2021","journal-title":"Stem Cells Transl. Med."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"622","DOI":"10.5966\/sctm.2016-0192","article-title":"Development of a Three-Dimensional Bioengineering Technology to Generate Lung Tissue for Personalized Disease Modeling","volume":"6","author":"Wilkinson","year":"2017","journal-title":"Stem Cells Transl. Med."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1016\/j.stemcr.2017.11.001","article-title":"Accelerated and Improved Differentiation of Retinal Organoids from Pluripotent Stem Cells in Rotating-Wall Vessel Bioreactors","volume":"10","author":"DiStefano","year":"2018","journal-title":"Stem Cell Rep."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"316","DOI":"10.1007\/s11626-998-0008-y","article-title":"Simulated Microgravity Conditions Enhance Differentiation of Cultured PC12 Cells towards the Neuroendocrine Phenotype","volume":"34","author":"Lelkes","year":"1998","journal-title":"Cell Dev. Biol. Anim."},{"key":"ref_67","unstructured":"Lelkes, P.I., Akhtar, N., Lelkes, E., Maltz, L., Arthur, R., Wiederholt, J., and Unsworth, B.R. Proceedings of the 2001 Conference Proceedings of the 23rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society, Istanbul, Turkey, 25\u201328 October 2001."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1089\/ten.tec.2019.0088","article-title":"An Air Bubble-Isolating Rotating Wall Vessel Bioreactor for Improved Spheroid\/Organoid Formation","volume":"25","author":"Phelan","year":"2019","journal-title":"Tissue Eng. Part. C Methods"},{"key":"ref_69","first-page":"3316","article-title":"Customized Rotating Wall Vessel Bioreactors Produce Improved Retinal Organoids with Reduced Operational Costs and Less Frequent Experimental Failure","volume":"60","author":"Phelan","year":"2019","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"960","DOI":"10.1016\/j.stemcr.2019.10.006","article-title":"Effects of Spaceflight on Human Induced Pluripotent Stem Cell-Derived Cardiomyocyte Structure and Function","volume":"13","author":"Wnorowski","year":"2019","journal-title":"Stem Cell Rep."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"2615","DOI":"10.1007\/s00264-014-2454-3","article-title":"Simulated Microgravity Affects Chondrogenesis and Hypertrophy of Human Mesenchymal Stem Cells","volume":"38","author":"Hammerschmid","year":"2014","journal-title":"Int. Orthop."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.actaastro.2011.07.028","article-title":"Cytoskeletal Proteins and Stem Cell Markers Gene Expression in Human Bone Marrow Mesenchymal Stromal Cells after Different Periods of Simulated Microgravity","volume":"70","author":"Gershovich","year":"2012","journal-title":"Acta Astronaut."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1038\/s41526-021-00158-4","article-title":"Update on the Effects of Microgravity on the Musculoskeletal System","volume":"7","author":"Juhl","year":"2021","journal-title":"npj Microgravity"},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"100337","DOI":"10.1016\/j.crmeth.2022.100337","article-title":"Suspension Culture in a Rotating Bioreactor for Efficient Generation of Human Intestinal Organoids","volume":"2","author":"Takahashi","year":"2022","journal-title":"Cell Rep. Methods"},{"key":"ref_75","unstructured":"(2023, March 19). \u201cCELLFLOAT\u201d Products\u2014J-Tec Corporation. Available online: https:\/\/www.j-tec.co.jp\/english\/life-science\/cellfloat\/."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"H168","DOI":"10.1152\/ajpheart.2001.280.1.H168","article-title":"Tissue Engineering of Functional Cardiac Muscle: Molecular, Structural, and Electrophysiological Studies","volume":"280","author":"Papadaki","year":"2001","journal-title":"Am. J. Physiol. Heart Circ. Physiol."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1186\/s40824-019-0176-8","article-title":"Electrical Stimulation as a Novel Tool for Regulating Cell Behavior in Tissue Engineering","volume":"23","author":"Chen","year":"2019","journal-title":"Biomater. Res."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1146\/annurev.bioeng.2.1.377","article-title":"New Currents in Electrical Stimulation of Excitable Tissues1","volume":"2","author":"Basser","year":"2003","journal-title":"Annu. Rev. Biomed. Eng."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1146\/annurev-bioeng-071114-040647","article-title":"Endogenous Bioelectric Signaling Networks: Exploiting Voltage Gradients for Control of Growth and Form","volume":"19","author":"Levin","year":"2017","journal-title":"Annu. Rev. Biomed. Eng."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1089\/bioe.2020.0027","article-title":"Endogenous Electric Signaling as a Blueprint for Conductive Materials in Tissue Engineering","volume":"3","author":"Casella","year":"2020","journal-title":"Bioelectricity"},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"314","DOI":"10.4028\/www.scientific.net\/AMM.890.314","article-title":"Electrical Stimulation Optimization in Bioreactors for Tissue Engineering Applications","volume":"890","author":"Datta","year":"2019","journal-title":"Appl. Mech. Mater."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"3311","DOI":"10.1152\/jn.01168.2005","article-title":"Electrical Stimulation of Mammalian Retinal Ganglion Cells with Multielectrode Arrays","volume":"95","author":"Sekirnjak","year":"2006","journal-title":"J. Neurophysiol."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"5031","DOI":"10.1126\/sciadv.abq5031","article-title":"Shell Microelectrode Arrays (MEAs) for Brain Organoids","volume":"8","author":"Huang","year":"2022","journal-title":"Sci. Adv."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1038\/s41569-019-0331-x","article-title":"Cardiomyocyte Maturation: Advances in Knowledge and Implications for Regenerative Medicine","volume":"17","author":"Karbassi","year":"2020","journal-title":"Nat. Rev. Cardiol."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"10312","DOI":"10.1038\/ncomms10312","article-title":"Autonomous Beating Rate Adaptation in Human Stem Cell-Derived Cardiomyocytes","volume":"7","author":"Eng","year":"2016","journal-title":"Nat. Commun."},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"1900060","DOI":"10.1002\/mabi.201900060","article-title":"Hydrogel Microchambers Integrated with Organic Electrodes for Efficient Electrical Stimulation of Human IPSC-Derived Cardiomyocytes","volume":"19","author":"Yoshida","year":"2019","journal-title":"Macromol. Biosci."},{"key":"ref_87","unstructured":"(2023, January 31). Culture Pacing System\u2014IonOptix. Available online: https:\/\/www.ionoptix.com\/products\/systems\/cell-culture-pacing\/."},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1089\/ars.2016.6766","article-title":"Electrical Stimulation Enhances Cardiac Differentiation of Human Induced Pluripotent Stem Cells for Myocardial Infarction Therapy","volume":"28","author":"Ma","year":"2018","journal-title":"Antioxid. Redox Signal"},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"9855","DOI":"10.1038\/s41598-018-27784-5","article-title":"Electrical Stimulation with a Conductive Polymer Promotes Neurite Outgrowth and Synaptogenesis in Primary Cortical Neurons in 3D","volume":"8","author":"Zhang","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1523\/ENEURO.0273-20.2020","article-title":"Electric Field Application In Vivo Regulates Neural Precursor Cell Behavior in the Adult Mammalian Forebrain","volume":"7","author":"Sefton","year":"2020","journal-title":"eNeuro"},{"key":"ref_91","doi-asserted-by":"crossref","unstructured":"LaBarge, W., Mattappally, S., Kannappan, R., Fast, V.G., Pretorius, D., Berry, J.L., and Zhang, J. (2019). Maturation of Three-Dimensional, HiPSC-Derived Cardiomyocyte Spheroids Utilizing Cyclic, Uniaxial Stretch and Electrical Stimulation. PLoS ONE, 14.","DOI":"10.1371\/journal.pone.0219442"},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1007\/s10544-017-0184-1","article-title":"Carbon Nanotubes Embedded in Embryoid Bodies Direct Cardiac Differentiation","volume":"19","author":"Ahadian","year":"2017","journal-title":"Biomed. Microdevices"},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"7075","DOI":"10.1039\/C5NR07059G","article-title":"Graphene Induces Spontaneous Cardiac Differentiation in Embryoid Bodies","volume":"8","author":"Ahadian","year":"2016","journal-title":"Nanoscale"},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"e115","DOI":"10.1002\/term.377","article-title":"Optimization of Electrical Stimulation Parameters for Cardiac Tissue Engineering","volume":"5","author":"Tandon","year":"2011","journal-title":"J. Tissue Eng. Regen. Med."},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"1417","DOI":"10.1089\/ten.tec.2010.0068","article-title":"Electric Field Stimulation Integrated into Perfusion Bioreactor for Cardiac Tissue Engineering","volume":"16","author":"Barash","year":"2010","journal-title":"Tissue Eng. Part. C Methods"},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1038\/s41586-018-0016-3","article-title":"Advanced Maturation of Human Cardiac Tissue Grown from Pluripotent Stem Cells","volume":"556","author":"Ma","year":"2018","journal-title":"Nature"},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"16944","DOI":"10.1038\/s41598-018-35019-w","article-title":"Enhancing All-in-One Bioreactors by Combining Interstitial Perfusion, Electrical Stimulation, on-Line Monitoring and Testing within a Single Chamber for Cardiac Constructs","volume":"8","author":"Visone","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_98","doi-asserted-by":"crossref","first-page":"1557","DOI":"10.1161\/CIRCULATIONAHA.114.014998","article-title":"Mechanical Stress Conditioning and Electrical Stimulation Promote Contractility and Force Maturation of Induced Pluripotent Stem Cell-Derived Human Cardiac Tissue","volume":"134","author":"Ruan","year":"2016","journal-title":"Circulation"},{"key":"ref_99","doi-asserted-by":"crossref","first-page":"717","DOI":"10.1007\/s12551-021-00841-6","article-title":"Current Strategies of Mechanical Stimulation for Maturation of Cardiac Microtissues","volume":"13","author":"Occhetta","year":"2021","journal-title":"Biophys. Rev."},{"key":"ref_100","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1007\/s42242-021-00154-3","article-title":"Bioreactor Design and Validation for Manufacturing Strategies in Tissue Engineering","volume":"5","author":"Lim","year":"2022","journal-title":"Bio-Des. Manuf."},{"key":"ref_101","doi-asserted-by":"crossref","first-page":"3","DOI":"10.5176\/2251-3140_3.2.52","article-title":"Microwaves: An Alternative Bacterial Sterilization Technique?","volume":"3","author":"Sood","year":"2015","journal-title":"GSTF J. BioSciences"},{"key":"ref_102","unstructured":"Atala, A. (2002). Methods of Tissue Engineering, Academic Press."},{"key":"ref_103","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1007\/112_2020_47","article-title":"Single-Cell Sequencing and Organoids: A Powerful Combination for Modelling Organ Development and Diseases","volume":"Volume 179","author":"Yin","year":"2021","journal-title":"Reviews of Physiology, Biochemistry and Pharmacology"},{"key":"ref_104","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1038\/s41392-022-01024-9","article-title":"Human Organoids in Basic Research and Clinical Applications","volume":"7","author":"Tang","year":"2022","journal-title":"Signal Transduct. Target. Ther."},{"key":"ref_105","doi-asserted-by":"crossref","first-page":"920","DOI":"10.1126\/science.aao2774","article-title":"Patient-Derived Organoids Model Treatment Response of Metastatic Gastrointestinal Cancers","volume":"359","author":"Vlachogiannis","year":"2018","journal-title":"Science"},{"key":"ref_106","doi-asserted-by":"crossref","first-page":"718","DOI":"10.1007\/s12015-021-10147-5","article-title":"Culture Variabilities of Human IPSC-Derived Cerebral Organoids Are a Major Issue for the Modelling of Phenotypes Observed in Alzheimer\u2019s Disease","volume":"18","author":"Rooney","year":"2022","journal-title":"Stem Cell Rev. Rep."},{"key":"ref_107","doi-asserted-by":"crossref","first-page":"935","DOI":"10.1002\/stem.1060","article-title":"Prolonged Maturation Culture Favors a Reduction in the Tumorigenicity and the Dopaminergic Function of Human ESC-Derived Neural Cells in a Primate Model of Parkinson\u2019s Disease","volume":"30","author":"Doi","year":"2012","journal-title":"Stem Cells"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/14\/11427\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T20:11:32Z","timestamp":1760127092000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/14\/11427"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,7,13]]},"references-count":107,"journal-issue":{"issue":"14","published-online":{"date-parts":[[2023,7]]}},"alternative-id":["ijms241411427"],"URL":"https:\/\/doi.org\/10.3390\/ijms241411427","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,7,13]]}}}