{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T00:38:39Z","timestamp":1774485519003,"version":"3.50.1"},"reference-count":46,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2023,7,19]],"date-time":"2023-07-19T00:00:00Z","timestamp":1689724800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Korea Health Industry Development Institute (KHIDI)","award":["HV20C0097"],"award-info":[{"award-number":["HV20C0097"]}]},{"name":"Korea Health Industry Development Institute (KHIDI)","award":["HV22C0159"],"award-info":[{"award-number":["HV22C0159"]}]},{"DOI":"10.13039\/501100003710","name":"Ministry of Health &amp; Welfare, Republic of Korea","doi-asserted-by":"publisher","award":["HV20C0097"],"award-info":[{"award-number":["HV20C0097"]}],"id":[{"id":"10.13039\/501100003710","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003710","name":"Ministry of Health &amp; Welfare, Republic of Korea","doi-asserted-by":"publisher","award":["HV22C0159"],"award-info":[{"award-number":["HV22C0159"]}],"id":[{"id":"10.13039\/501100003710","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The current production of the Japanese encephalitis virus (JEV) vaccine is based on animal cells, where various risk factors for human health should be resolved. This study used a transient expression system to express the chimeric protein composed of antigenic epitopes from the JEV envelope (E) protein in Nicotiana benthamiana. JEV multi-epitope peptide (MEP) sequences fused with FLAG-tag or 6\u00d7 His-tag at the C- or N-terminus for the purification were introduced into plant expression vectors and used for transient expression. Among the constructs, vector pSK480, which expresses MEP fused with a FLAG-tag at the C-terminus, showed the highest level of expression and yield in purification. Optimization of transient expression procedures further improved the target protein yield. The purified MEP protein was applied to an ICR mouse and successfully induced an antibody against JEV, which demonstrates the potential of the plant-produced JEV MEP as an alternative vaccine candidate.<\/jats:p>","DOI":"10.3390\/ijms241411643","type":"journal-article","created":{"date-parts":[[2023,7,19]],"date-time":"2023-07-19T21:20:14Z","timestamp":1689801614000},"page":"11643","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Production of Plant-Derived Japanese Encephalitis Virus Multi-Epitope Peptide in Nicotiana benthamiana and Immunological Response in Mice"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2708-0704","authenticated-orcid":false,"given":"Jae-Wan","family":"Jung","sequence":"first","affiliation":[{"name":"Department of Life Science, Sogang University, Seoul 04107, Republic of Korea"},{"name":"PhytoMab Co., Seoul 04107, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3024-3439","authenticated-orcid":false,"given":"Pil-Gu","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Gachon University College of Medicine, Incheon 21936, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Won-Kyung","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Life Science, Sogang University, Seoul 04107, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun-Hye","family":"Shin","sequence":"additional","affiliation":[{"name":"Department of Life Science, Sogang University, Seoul 04107, Republic of Korea"},{"name":"PhytoMab Co., Seoul 04107, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mi-Hwa","family":"Jang","sequence":"additional","affiliation":[{"name":"Department of Life Science, Sogang University, Seoul 04107, Republic of Korea"},{"name":"PhytoMab Co., Seoul 04107, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2831-8267","authenticated-orcid":false,"given":"Eun-Hye","family":"Seo","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Gachon University College of Medicine, Incheon 21936, Republic of Korea"},{"name":"BK21 Plus, Department of Cellular and Molecular Medicine, Konkuk University School of Medicine, Seoul 05029, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Timothy","family":"An","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Gachon University College of Medicine, Incheon 21936, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Young Beom","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Yonsei University, Seoul 03722, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5454-2601","authenticated-orcid":false,"given":"Myeong Hee","family":"Moon","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Yonsei University, Seoul 03722, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seuk-Keun","family":"Choi","sequence":"additional","affiliation":[{"name":"Eubiologics Co., Seoul 06026, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jee Sun","family":"Yun","sequence":"additional","affiliation":[{"name":"Eubiologics Co., Seoul 06026, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kee-Jong","family":"Hong","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Gachon University College of Medicine, Incheon 21936, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seong-Ryong","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Life Science, Sogang University, Seoul 04107, Republic of Korea"},{"name":"PhytoMab Co., Seoul 04107, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,7,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"955","DOI":"10.4014\/jmb.2207.07002","article-title":"Emerging Japanese Encephalitis Virus Genotype V in Republic of Korea","volume":"32","author":"Lee","year":"2022","journal-title":"J. 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