{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T13:57:24Z","timestamp":1783605444597,"version":"3.55.0"},"reference-count":93,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2023,7,19]],"date-time":"2023-07-19T00:00:00Z","timestamp":1689724800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Oxidative stress is the most critical factor in multiple functional disorders\u2019 development, and natural antioxidants could protect the human body against it. Our study aims to investigate the polyphenol content of four extracts of two medicinal plants (Rosmarinus officinalis L. and Thymus vulgaris L.) and analyze the correlation with their antioxidant activity. The research was carried out on extracts of rosemary and thyme obtained from species cultivated together in plant communities. Both were compared with extracts from species cultivated in individual crops (control crops). Their polyphenols were determined by spectrophotometric methods (dosage of flavones, phenol carboxylic acids, and total polyphenols) and chromatography (UHPLC\u2013MS and FT\u2013ICR MS). Triterpenic acids were also quantified, having a higher concentration in the thyme extract from the culture. The antioxidant activity of the dry extracts was evaluated in vitro (DPPH, ABTS, and FRAP) and in silico (prediction of interactions with BACH1\/BACH2 transcription factors). The concentrations of polyphenols are higher in the extracts obtained from the sources collected from the common crops. These observations were also validated following the chromatographic analysis for some compounds. Statistically significant differences in the increase in the antioxidant effect were observed for the extracts from the common batches compared to those from the individual ones. Following the Pearson analysis, the IC50 values for each plant extract were strongly correlated with the concentration of active phytoconstituents. Molecular docking studies revealed that quercetin could bind to BTB domains of BACH1 and BACH2 transcription factors, likely translating into increased antioxidant enzyme expression. Future studies must validate the in silico findings and further investigate phytosociological cultivation\u2019s effects.<\/jats:p>","DOI":"10.3390\/ijms241411670","type":"journal-article","created":{"date-parts":[[2023,7,19]],"date-time":"2023-07-19T21:20:14Z","timestamp":1689801614000},"page":"11670","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Implications of the Cultivation of Rosemary and Thyme (Lamiaceae) in Plant Communities for the Development of Antioxidant Therapies"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6903-3260","authenticated-orcid":false,"given":"Emanuela-Alice","family":"Lu\u021b\u0103","sequence":"first","affiliation":[{"name":"Faculty of Pharmacy, University of Medicine and Pharmacy \u201cCarol Davila\u201d, Traian Vuia 6, 020956 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4715-1423","authenticated-orcid":false,"given":"Andrei","family":"Bi\u021b\u0103","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy & Phytotherapy, Faculty of Pharmacy, University of Medicine and Pharmacy of Craiova, Petru Rare\u0219 2, 200349 Craiova, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1437-6570","authenticated-orcid":false,"given":"Alina","family":"Moro\u0219an","sequence":"additional","affiliation":[{"name":"Department of Organic Chemistry \u201cCostin Neni\u021bescu\u201d, Faculty of Chemical Engineering and Biotechnologies, University of Politehnica, Gheorghe Polizu 1\u20137, 011061 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan Eduard","family":"Mihaiescu","sequence":"additional","affiliation":[{"name":"Department of Organic Chemistry \u201cCostin Neni\u021bescu\u201d, Faculty of Chemical Engineering and Biotechnologies, University of Politehnica, Gheorghe Polizu 1\u20137, 011061 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2880-9381","authenticated-orcid":false,"given":"Drago\u0219 Paul","family":"Mihai","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, University of Medicine and Pharmacy \u201cCarol Davila\u201d, Traian Vuia 6, 020956 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liliana","family":"Popescu","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, University of Medicine and Pharmacy \u201cCarol Davila\u201d, Traian Vuia 6, 020956 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ludovic Everard","family":"Bejenaru","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy & Phytotherapy, Faculty of Pharmacy, University of Medicine and Pharmacy of Craiova, Petru Rare\u0219 2, 200349 Craiova, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cornelia","family":"Bejenaru","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Botany, Faculty of Pharmacy, University of Medicine and Pharmacy of Craiova, Petru Rare\u0219 2, 200349 Craiova, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9696-5728","authenticated-orcid":false,"given":"Violeta","family":"Popovici","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Faculty of Dental Medicine, Ovidius University of Constanta, 7 Ilarie Voronca Street, 900684 Constanta, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6904-3066","authenticated-orcid":false,"given":"Octavian Tudorel","family":"Olaru","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, University of Medicine and Pharmacy \u201cCarol Davila\u201d, Traian Vuia 6, 020956 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4372-4335","authenticated-orcid":false,"given":"Cerasela Elena","family":"G\u00eerd","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, University of Medicine and Pharmacy \u201cCarol Davila\u201d, Traian Vuia 6, 020956 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,7,19]]},"reference":[{"key":"ref_1","first-page":"31","article-title":"The International Encyclopedia of Geography: People, the Earth, Environment and Technology","volume":"32","author":"Packard","year":"2018","journal-title":"Ref. 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