{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T04:29:01Z","timestamp":1788496141759,"version":"build-2803163510"},"reference-count":37,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2023,7,20]],"date-time":"2023-07-20T00:00:00Z","timestamp":1689811200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["U22A20505"],"award-info":[{"award-number":["U22A20505"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["2023AAC03100"],"award-info":[{"award-number":["2023AAC03100"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["2023AAC03201"],"award-info":[{"award-number":["2023AAC03201"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004772","name":"Natural Science Foundation of Ningxia","doi-asserted-by":"crossref","award":["U22A20505"],"award-info":[{"award-number":["U22A20505"]}],"id":[{"id":"10.13039\/501100004772","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004772","name":"Natural Science Foundation of Ningxia","doi-asserted-by":"crossref","award":["2023AAC03100"],"award-info":[{"award-number":["2023AAC03100"]}],"id":[{"id":"10.13039\/501100004772","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004772","name":"Natural Science Foundation of Ningxia","doi-asserted-by":"crossref","award":["2023AAC03201"],"award-info":[{"award-number":["2023AAC03201"]}],"id":[{"id":"10.13039\/501100004772","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.mdpi.com"],"crossmark-restriction":true},"short-container-title":["IJMS"],"abstract":"<jats:p>Tuberculosis (TB) is a zoonotic infectious disease caused by Mycobacterium tuberculosis (Mtb). Mtb is a typical intracellular parasite, and macrophages are its main host cells. NLRP3 inflammasome-mediated pyroptosis is a form of programmed cell death implicated in the clearance of pathogenic infections. The bidirectional regulatory effect of endoplasmic reticulum stress (ERS) plays a crucial role in determining cell survival and death. Whether ERS is involved in macrophage pyroptosis with Mtb infection remains unclear. This article aims to explore the regulation of the NLRP3 inflammasome and pyroptosis by ERS in THP-1 macrophages infected with Mycobacterium bovis Bacillus Calmette-Gu\u00e9rin (BCG). The results showed that BCG infection induced THP-1 macrophage ERS, NLRP3 inflammasome activation and pyroptosis, which was inhibited by ERS inhibitor TUDCA. NLRP3 inhibitor MCC950 inhibited THP-1 macrophage NLRP3 inflammasome activation and pyroptosis caused by BCG infection. Compared with specific Caspase-1 inhibitor VX-765, pan-Caspase inhibitor Z-VAD-FMK showed a more significant inhibitory effect on BCG infection-induced pyroptosis of THP-1 macrophages. Taken together, this study demonstrates that ERS mediated NLRP3 inflammasome activation and pyroptosis after BCG infection of THP-1 macrophages, and that BCG infection of THP-1 macrophages induces pyroptosis through canonical and noncanonical pathways.<\/jats:p>","DOI":"10.3390\/ijms241411692","type":"journal-article","created":{"date-parts":[[2023,7,20]],"date-time":"2023-07-20T04:27:13Z","timestamp":1689827233000},"page":"11692","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Endoplasmic Reticulum Stress Mediated NLRP3 Inflammasome Activation and Pyroptosis in THP-1 Macrophages Infected with Bacillus Calmette-Gu\u00e9rin"],"prefix":"10.3390","volume":"24","author":[{"given":"Xueyi","family":"Nie","sequence":"first","affiliation":[{"name":"School of Life Sciences, Ningxia University, Yinchuan 750021, China"},{"name":"Key Laboratory of Ministry of Education for Conservation and Utilization of Special 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China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuxin","family":"Hou","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Ningxia University, Yinchuan 750021, China"},{"name":"Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western, Ningxia University, Yinchuan 750021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6317-5460","authenticated-orcid":false,"given":"Yi","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Ningxia University, Yinchuan 750021, China"},{"name":"Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western, Ningxia University, Yinchuan 750021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9258-8002","authenticated-orcid":false,"given":"Jinrui","family":"Xu","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Ningxia University, Yinchuan 750021, China"},{"name":"Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western, Ningxia University, Yinchuan 750021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yujiong","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Ningxia University, Yinchuan 750021, China"},{"name":"Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western, Ningxia University, Yinchuan 750021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,7,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/j.tube.2018.05.014","article-title":"Antituberculous drugs modulate bacterial phagolysosome avoidance and autophagy in Mycobacterium tuberculosis-infected macrophages","volume":"111","author":"Genestet","year":"2018","journal-title":"Tuberculosis"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Gonzalez-Orozco, M., Strong, E.J., Paroha, R., and Lee, S. 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