{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T02:21:15Z","timestamp":1760149275898,"version":"build-2065373602"},"reference-count":53,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2023,7,24]],"date-time":"2023-07-24T00:00:00Z","timestamp":1690156800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100006827","name":"Florida Department of Health Grant\u2013James and Esther King Research Program","doi-asserted-by":"publisher","award":["#21K12","16SDG29120002","#046259"],"award-info":[{"award-number":["#21K12","16SDG29120002","#046259"]}],"id":[{"id":"10.13039\/100006827","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000968","name":"American Heart Association Scientist Development Grant","doi-asserted-by":"publisher","award":["#21K12","16SDG29120002","#046259"],"award-info":[{"award-number":["#21K12","16SDG29120002","#046259"]}],"id":[{"id":"10.13039\/100000968","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Florida State University Counsel for Creativity and PG","award":["#21K12","16SDG29120002","#046259"],"award-info":[{"award-number":["#21K12","16SDG29120002","#046259"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The protein sarcospan (SSPN) is an integral member of the dystrophin-glycoprotein complex (DGC) and has been shown to be important in the heart during the development and the response to acute stress. In this study, we investigated the role of SSPN in the cardiac response to acute ischemia-reperfusion (IR) injury in SSPN-deficient (SSPN\u2212\/\u2212) mice. First, the hemodynamic response of SSPN\u2212\/\u2212 mice was tested and was similar to SSPN+\/+ (wild-type) mice after isoproterenol injection. Using the in situ Langendorff perfusion method, SSPN\u2212\/\u2212 hearts were subjected to IR injury and found to have increased infarct size and arrhythmia susceptibility compared to SSPN+\/+. Ca2+ handling was assessed in single cardiomyocytes and diastolic Ca2+ levels were increased after acute \u03b2-AR stimulation in SSPN+\/+ but not SSPN\u2212\/\u2212. It was also found that SSPN\u2212\/\u2212 cardiomyocytes had reduced Ca2+ SR content compared to SSPN+\/+ but similar SR Ca2+ release. Next, we used qRT-PCR to examine gene expression of Ca2+ handling proteins after acute IR injury. SSPN\u2212\/\u2212 hearts showed a significant decrease in L-type Ca2+ channels and a significant increase in Ca2+ release channel (RyR2) expression. Interestingly, under oxidizing conditions reminiscent of IR, SSPN\u2212\/\u2212 cardiomyocytes, had increased H2O2-induced reactive oxygen species production compared to SSPN+\/+. Examination of oxidative stress proteins indicated that NADPH oxidase 4 and oxidized CAMKII were increased in SSPN\u2212\/\u2212 hearts after acute IR injury. These results suggest that increased arrhythmia susceptibility in SSPN\u2212\/\u2212 hearts post-IR injury may arise from alterations in Ca2+ handling and a reduced capacity to regulate oxidative stress pathways.<\/jats:p>","DOI":"10.3390\/ijms241411868","type":"journal-article","created":{"date-parts":[[2023,7,25]],"date-time":"2023-07-25T01:21:27Z","timestamp":1690248087000},"page":"11868","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Sarcospan Deficiency Increases Oxidative Stress and Arrhythmias in Hearts after Acute Ischemia-Reperfusion Injury"],"prefix":"10.3390","volume":"24","author":[{"given":"Hyun Seok","family":"Hwang","sequence":"first","affiliation":[{"name":"Department of Nutrition and Integrative Physiology, Florida State University, 107 Chieftan Way, Biomedical Research Facility, Tallahassee, FL 32306-1490, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6319-9614","authenticated-orcid":false,"given":"Aida Rahimi","family":"Kahmini","sequence":"additional","affiliation":[{"name":"Department of Nutrition and Integrative Physiology, Florida State University, 107 Chieftan Way, Biomedical Research Facility, Tallahassee, FL 32306-1490, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julia","family":"Prascak","sequence":"additional","affiliation":[{"name":"Department of Nutrition and Integrative Physiology, Florida State University, 107 Chieftan Way, Biomedical Research Facility, Tallahassee, FL 32306-1490, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexis","family":"Cejas-Carbonell","sequence":"additional","affiliation":[{"name":"Department of Nutrition and Integrative Physiology, Florida State University, 107 Chieftan Way, Biomedical Research Facility, Tallahassee, FL 32306-1490, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8610-0480","authenticated-orcid":false,"given":"Isela C.","family":"Valera","sequence":"additional","affiliation":[{"name":"Department of Nutrition and Integrative Physiology, Florida State University, 107 Chieftan Way, Biomedical Research Facility, Tallahassee, FL 32306-1490, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samantha","family":"Champion","sequence":"additional","affiliation":[{"name":"Department of Nutrition and Integrative Physiology, Florida State University, 107 Chieftan Way, Biomedical Research Facility, Tallahassee, FL 32306-1490, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mikayla","family":"Corrigan","sequence":"additional","affiliation":[{"name":"Department of Nutrition and Integrative Physiology, Florida State University, 107 Chieftan Way, Biomedical Research Facility, Tallahassee, FL 32306-1490, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Florence","family":"Mumbi","sequence":"additional","affiliation":[{"name":"Department of Nutrition and Integrative Physiology, Florida State University, 107 Chieftan Way, Biomedical Research Facility, Tallahassee, FL 32306-1490, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9416-0069","authenticated-orcid":false,"given":"Michelle S.","family":"Parvatiyar","sequence":"additional","affiliation":[{"name":"Department of Nutrition and Integrative Physiology, Florida State University, 107 Chieftan Way, Biomedical Research Facility, Tallahassee, FL 32306-1490, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,7,24]]},"reference":[{"doi-asserted-by":"crossref","unstructured":"Tsuda, T., and Fitzgerald, K.K. 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