{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,20]],"date-time":"2026-03-20T05:54:28Z","timestamp":1773986068111,"version":"3.50.1"},"reference-count":35,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2023,7,25]],"date-time":"2023-07-25T00:00:00Z","timestamp":1690243200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Instituto de Salud Carlos III","award":["PI18\/00947"],"award-info":[{"award-number":["PI18\/00947"]}]},{"name":"Instituto de Salud Carlos III","award":["AC18\/00033"],"award-info":[{"award-number":["AC18\/00033"]}]},{"name":"Instituto de Salud Carlos III","award":["PI21\/00691"],"award-info":[{"award-number":["PI21\/00691"]}]},{"name":"European Union","award":["PI18\/00947"],"award-info":[{"award-number":["PI18\/00947"]}]},{"name":"European Union","award":["AC18\/00033"],"award-info":[{"award-number":["AC18\/00033"]}]},{"name":"European Union","award":["PI21\/00691"],"award-info":[{"award-number":["PI21\/00691"]}]},{"name":"ISCIII","award":["PI18\/00947"],"award-info":[{"award-number":["PI18\/00947"]}]},{"name":"ISCIII","award":["AC18\/00033"],"award-info":[{"award-number":["AC18\/00033"]}]},{"name":"ISCIII","award":["PI21\/00691"],"award-info":[{"award-number":["PI21\/00691"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Molecular markers of dedifferentiation of dysfunctional liver sinusoidal endothelial cells (LSEC) have not been fully elucidated. We aimed at deciphering the molecular profile of dysfunctional LSEC in different pathological scenarios. Flow cytometry was used to sort CD11b\u2212\/CD32b+ and CD11b\u2212\/CD32b\u2212 LSEC from three rat models of liver disease (bile duct ligation-BDL; inhaled carbon tetrachloride-CCl4; and high fat glucose\/fructose diet-HFGFD). A full proteomic profile was performed applying nano-scale liquid chromatography tandem mass spectrometry (nLC-MS) and analyzed with PEAKS software. The percentage of CD32b\u2212 LSEC varied across groups, suggesting different capillarization processes. Both CD32+ and CD32b\u2212 LSEC from models are different from control LSEC, but differently expressed proteins in CD32b\u2212 LSEC are significantly higher. Heatmaps evidenced specific protein expression patterns for each model. Analysis of biological significance comparing dysfunctional CD32b\u2212 LSEC with specialized CD32b+ LSEC from controls showed central similarities represented by 45 common down-regulated proteins involved in the suppression of the endocytic machinery and 63 common up-regulated proteins associated with the actin-dependent cytoskeleton reorganization. In summary; substantial differences but also similarities in dysfunctional LSEC from the three most common models of liver disease were found, supporting the idea that LSEC may harbor different protein expression profiles according to the etiology or disease stage.<\/jats:p>","DOI":"10.3390\/ijms241511904","type":"journal-article","created":{"date-parts":[[2023,7,26]],"date-time":"2023-07-26T00:45:02Z","timestamp":1690332302000},"page":"11904","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Proteomic Analysis of Dysfunctional Liver Sinusoidal Endothelial Cells Reveals Substantial Differences in Most Common Experimental Models of Chronic Liver Diseases"],"prefix":"10.3390","volume":"24","author":[{"given":"Mar","family":"Gil","sequence":"first","affiliation":[{"name":"Liver Diseases, Vall d\u2019Hebron Institut de Recerca (VHIR), Liver Unit, Hospital Universitari Vall d\u2019Hebron (HUVH), Vall d\u2019Hebron Barcelona Hospital Campus, Universitat Aut\u00f2noma de Barcelona (UAB), 08035 Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mikel","family":"Azkargorta","sequence":"additional","affiliation":[{"name":"Proteomics Platform, CIC bioGUNE, BRTA (Basque Research & Technology Alliance), Bizkaia Science and Technology Park, 48160 Derio, Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Hep\u00e1ticas y Digestivas (CIBERehd), 28029 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carla","family":"Fuster","sequence":"additional","affiliation":[{"name":"Liver Diseases, Vall d\u2019Hebron Institut de Recerca (VHIR), Liver Unit, Hospital Universitari Vall d\u2019Hebron (HUVH), Vall d\u2019Hebron Barcelona Hospital Campus, Universitat Aut\u00f2noma de Barcelona (UAB), 08035 Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda","family":"Mart\u00ednez-G\u00f3mez","sequence":"additional","affiliation":[{"name":"Liver Diseases, Vall d\u2019Hebron Institut de Recerca (VHIR), Liver Unit, Hospital Universitari Vall d\u2019Hebron (HUVH), Vall d\u2019Hebron Barcelona Hospital Campus, Universitat Aut\u00f2noma de Barcelona (UAB), 08035 Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Imma","family":"Raurell","sequence":"additional","affiliation":[{"name":"Liver Diseases, Vall d\u2019Hebron Institut de Recerca (VHIR), Liver Unit, Hospital Universitari Vall d\u2019Hebron (HUVH), Vall d\u2019Hebron Barcelona Hospital Campus, Universitat Aut\u00f2noma de Barcelona (UAB), 08035 Barcelona, Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Hep\u00e1ticas y Digestivas (CIBERehd), 28029 Madrid, 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Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diana","family":"Hide","sequence":"additional","affiliation":[{"name":"Liver Diseases, Vall d\u2019Hebron Institut de Recerca (VHIR), Liver Unit, Hospital Universitari Vall d\u2019Hebron (HUVH), Vall d\u2019Hebron Barcelona Hospital Campus, Universitat Aut\u00f2noma de Barcelona (UAB), 08035 Barcelona, Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Hep\u00e1ticas y Digestivas (CIBERehd), 28029 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8839-5438","authenticated-orcid":false,"given":"Felix","family":"Elortza","sequence":"additional","affiliation":[{"name":"Proteomics Platform, CIC bioGUNE, BRTA (Basque Research & Technology Alliance), Bizkaia Science and Technology Park, 48160 Derio, Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Hep\u00e1ticas y Digestivas (CIBERehd), 28029 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Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Hep\u00e1ticas y Digestivas (CIBERehd), 28029 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,7,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1749","DOI":"10.1016\/S0140-6736(14)60121-5","article-title":"Liver cirrhosis","volume":"383","author":"Tsochatzis","year":"2014","journal-title":"Lancet"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1605","DOI":"10.1002\/hep.31173","article-title":"Changes in the Global Burden of Chronic Liver Diseases from 2012 to 2017: The Growing Impact of NAFLD","volume":"72","author":"Paik","year":"2020","journal-title":"Hepatology"},{"key":"ref_3","first-page":"7","article-title":"How to face chronic liver disease: The sinusoidal perspective","volume":"4","year":"2017","journal-title":"Front. 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