{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T08:20:45Z","timestamp":1770970845491,"version":"3.50.1"},"reference-count":25,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2023,7,25]],"date-time":"2023-07-25T00:00:00Z","timestamp":1690243200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002241","name":"JST-Mirai Program","doi-asserted-by":"publisher","award":["JPMJMI18CB"],"award-info":[{"award-number":["JPMJMI18CB"]}],"id":[{"id":"10.13039\/501100002241","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002241","name":"JST-Mirai Program","doi-asserted-by":"publisher","award":["JP21H03328"],"award-info":[{"award-number":["JP21H03328"]}],"id":[{"id":"10.13039\/501100002241","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002241","name":"JST-Mirai Program","doi-asserted-by":"publisher","award":["JP21be0804001"],"award-info":[{"award-number":["JP21be0804001"]}],"id":[{"id":"10.13039\/501100002241","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007132","name":"Otsuka Holdings Co., Ltd.","doi-asserted-by":"publisher","award":["JPMJMI18CB"],"award-info":[{"award-number":["JPMJMI18CB"]}],"id":[{"id":"10.13039\/501100007132","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007132","name":"Otsuka Holdings Co., Ltd.","doi-asserted-by":"publisher","award":["JP21H03328"],"award-info":[{"award-number":["JP21H03328"]}],"id":[{"id":"10.13039\/501100007132","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007132","name":"Otsuka Holdings Co., Ltd.","doi-asserted-by":"publisher","award":["JP21be0804001"],"award-info":[{"award-number":["JP21be0804001"]}],"id":[{"id":"10.13039\/501100007132","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Takeda Science Foundation","award":["JPMJMI18CB"],"award-info":[{"award-number":["JPMJMI18CB"]}]},{"name":"Takeda Science Foundation","award":["JP21H03328"],"award-info":[{"award-number":["JP21H03328"]}]},{"name":"Takeda Science Foundation","award":["JP21be0804001"],"award-info":[{"award-number":["JP21be0804001"]}]},{"name":"Japan Agency for Medical Research and Development, AMED","award":["JPMJMI18CB"],"award-info":[{"award-number":["JPMJMI18CB"]}]},{"name":"Japan Agency for Medical Research and Development, AMED","award":["JP21H03328"],"award-info":[{"award-number":["JP21H03328"]}]},{"name":"Japan Agency for Medical Research and Development, AMED","award":["JP21be0804001"],"award-info":[{"award-number":["JP21be0804001"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Adipose stem and progenitor cells (ASPCs) have been isolated from humans and animals for use in regenerative medicine and therapy. However, knowledge of ASPCs in other species is limited. Particularly, ASPCs in livestock are expected to enhance the fat content and meat composition. In this study, we isolated bovine ASPCs using cell surface markers. Specifically, we focused on ASPC markers in humans and experimental animals, namely CD26, CD146, and CD54. Stromal vascular fraction cells from bovine fat were separated using flow cytometry before primary culture. We evaluated the self-renewal and adipogenic potential of each fraction. We identified four cell populations: CD26\u2212CD146+CD54+, CD26\u2212CD146+CD54\u2212, CD26\u2212CD146\u2212, and CD26+CD146\u2212. Among them, the CD26\u2212CD146+ fraction, particularly CD54+, demonstrated the properties of preadipocytes (PreAs), characterized by slow proliferation and a high adipogenic capacity. In conclusion, we could collect and characterize possible PreAs as CD26\u2212CD146+CD54+ or CD26\u2212CD146+CD54\u2212, which are expected for in vitro bovine adipogenic assays in the future.<\/jats:p>","DOI":"10.3390\/ijms241511908","type":"journal-article","created":{"date-parts":[[2023,7,26]],"date-time":"2023-07-26T00:45:02Z","timestamp":1690332302000},"page":"11908","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Conservation of Markers and Stemness in Adipose Stem and Progenitor Cells between Cattle and Other Species"],"prefix":"10.3390","volume":"24","author":[{"given":"Yuki","family":"Ishida","sequence":"first","affiliation":[{"name":"Intractable Disease Research Center, Juntendo University Graduate School of Medicine, 2-1-1, Hongo, Bunkyo-ku, Tokyo 113-8421, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yo","family":"Mabuchi","sequence":"additional","affiliation":[{"name":"Intractable Disease Research Center, Juntendo University Graduate School of Medicine, 2-1-1, Hongo, Bunkyo-ku, Tokyo 113-8421, Japan"},{"name":"Department of Clinical Regenerative Medicine, Fujita Health University, Toyoake 470-1192, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7745-7944","authenticated-orcid":false,"given":"Yuna","family":"Naraoka","sequence":"additional","affiliation":[{"name":"Intractable Disease Research Center, Juntendo University Graduate School of Medicine, 2-1-1, Hongo, Bunkyo-ku, Tokyo 113-8421, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9075-8533","authenticated-orcid":false,"given":"Daisuke","family":"Hisamatsu","sequence":"additional","affiliation":[{"name":"Intractable Disease Research Center, Juntendo University Graduate School of Medicine, 2-1-1, Hongo, Bunkyo-ku, Tokyo 113-8421, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chihiro","family":"Akazawa","sequence":"additional","affiliation":[{"name":"Intractable Disease Research Center, Juntendo University Graduate School of Medicine, 2-1-1, Hongo, Bunkyo-ku, Tokyo 113-8421, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,7,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"937","DOI":"10.1016\/j.tcb.2020.09.007","article-title":"Toward a Consensus View of Mammalian Adipocyte Stem and Progenitor Cell Heterogeneity","volume":"30","author":"Ferrero","year":"2020","journal-title":"Trends Cell Biol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"53","DOI":"10.4238\/2015.January.15.7","article-title":"Generation of Bovine (Bos indicus) and Buffalo (Bubalus bubalis) Adipose Tissue Derived Stem Cells: Isolation, Characterization, and Multipotentiality","volume":"14","author":"Sampaio","year":"2015","journal-title":"Genet. 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