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Increasing evidence suggests that the exchange of extracellular vesicles (EVs) of both embryonic and endometrial origin is a critical component of embryo\u2013maternal communication during peri-implantation. Here, we investigated whether embryonic signals in the form of EVs can modulate the endometrial epithelial cell secretome. Receptive endometrial analog RL95-2 cells were supplemented with trophoblast analog JAr cell-derived EVs, and the secretory protein changes occurring in the RL95-2 cells were analyzed using mass spectrometry. EVs of non-trophoblastic origin (HEK 293 cells) were used as the control EV source to supplement endometrial cells. Trophoblast cell-derived EVs enriched endometrial epithelial cell secretions with proteins that support embryo development, attachment, or implantation, whereas control EVs were unable to induce the same effect. The present study suggests that embryonic signals in the form of EVs may prime receptive endometrial epithelial cells to enrich their secretory proteome with critical proteomic molecules with functional importance for periconception milieu formation.<\/jats:p>","DOI":"10.3390\/ijms241511924","type":"journal-article","created":{"date-parts":[[2023,7,26]],"date-time":"2023-07-26T00:45:02Z","timestamp":1690332302000},"page":"11924","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Secretory Proteomic Responses of Endometrial Epithelial Cells to Trophoblast-Derived Extracellular Vesicles"],"prefix":"10.3390","volume":"24","author":[{"given":"Subhashini","family":"Muhandiram","sequence":"first","affiliation":[{"name":"Institute of Veterinary Medicine and Animal Sciences, Estonian University of Life Sciences, Kreutzwaldi 62, 51006 Tartu, Estonia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keerthie","family":"Dissanayake","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Medicine and Animal Sciences, Estonian University of Life Sciences, Kreutzwaldi 62, 51006 Tartu, Estonia"},{"name":"Department of Pathophysiology, Institute of Biomedicine and Translational Medicine, University of Tartu, Ravila St. 14B, 50411 Tartu, Estonia"},{"name":"Department of Anatomy, Faculty of Medicine, University of Peradeniya, Kandy 20400, Sri Lanka"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toomos","family":"Orro","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Medicine and Animal Sciences, Estonian University of Life Sciences, Kreutzwaldi 62, 51006 Tartu, Estonia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kasun","family":"Godakumara","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Medicine and Animal Sciences, Estonian University of Life Sciences, Kreutzwaldi 62, 51006 Tartu, Estonia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suranga","family":"Kodithuwakku","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Medicine and Animal Sciences, Estonian University of Life Sciences, Kreutzwaldi 62, 51006 Tartu, Estonia"},{"name":"Department of Animal Science, Faculty of Agriculture, University of Peradeniya, Kandy 20400, Sri Lanka"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alireza","family":"Fazeli","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Medicine and Animal Sciences, Estonian University of Life Sciences, Kreutzwaldi 62, 51006 Tartu, Estonia"},{"name":"Department of Pathophysiology, Institute of Biomedicine and Translational Medicine, University of Tartu, Ravila St. 14B, 50411 Tartu, Estonia"},{"name":"Academic Unit of Reproductive and Developmental Medicine, Department of Oncology and Metabolism, Medical School, University of Sheffield, Sheffield S10 2TN, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,7,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1747","DOI":"10.1038\/s41598-021-81439-6","article-title":"Efficacy of Therapies and Interventions for Repeated Embryo Implantation Failure: A Systematic Review and Meta-Analysis","volume":"11","author":"Busnelli","year":"2021","journal-title":"Sci. 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