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Because fibrosis has a suspected basis of genetic predisposition, we have undertaken a prospective study to identify upregulated profibrotic genes in a population of glaucoma patients with signs of conjunctival fibrosis and early postoperative surgical failure. Clinical data of re-operated fibrosis patients, hyperfibrosis patients who re-operated more than once in a short time, and control patients with no fibrosis were recorded and analyzed at each follow-up visit. Conjunctival-Tenon surgical specimens were obtained intraoperatively to evaluate the local expression of a panel of genes potentially associated with fibrosis. In order to correlate gene expression signatures with protein levels, we quantified secreted proteins in primary cultures of fibroblasts from patients. Expression of VEGFA, CXCL8, MYC, and CDKN1A was induced in the conjunctiva of hyperfibrosis patients. VEGFA and IL8 protein levels were also increased in fibroblast supernatants. We propose that an increase in these proteins could be useful in detecting conjunctival fibrosis in glaucoma patients undergoing filtering surgery. Molecular markers could be crucial for early detection of patients at high risk of failure of filtration surgery, leading to more optimal and personalized treatments.<\/jats:p>","DOI":"10.3390\/ijms241511949","type":"journal-article","created":{"date-parts":[[2023,7,27]],"date-time":"2023-07-27T01:27:46Z","timestamp":1690421266000},"page":"11949","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Differential Gene and Protein Expression of Conjunctival Bleb Hyperfibrosis in Early Failure of Glaucoma Surgery"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5171-165X","authenticated-orcid":false,"given":"Elena","family":"Mill\u00e1","sequence":"first","affiliation":[{"name":"Hospital Cl\u00ednic de Barcelona, Institut Clinic d\u2019Oftalmologia, ICOF, Sabino Arana n\u00ba1, 08028 Barcelona, Spain"},{"name":"Institut Comtal d\u2019Oftalmologia, Innova Ocular-ICO Barcelona, Via Augusta 48, 08006 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5446-7675","authenticated-orcid":false,"given":"N\u00e9stor","family":"Ventura-Abreu","sequence":"additional","affiliation":[{"name":"Hospital Universitari Sagrat Cor., Viladomat 288, 08029 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-9274-0530","authenticated-orcid":false,"given":"Cristina","family":"Vendrell","sequence":"additional","affiliation":[{"name":"Institut Comtal d\u2019Oftalmologia, Innova Ocular-ICO Barcelona, Via Augusta 48, 08006 Barcelona, Spain"},{"name":"Hospital de Viladecans, Avda. Gav\u00e0 38, 08840 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Jes\u00fas","family":"Muniesa","sequence":"additional","affiliation":[{"name":"Hospital Cl\u00ednic de Barcelona, Institut Clinic d\u2019Oftalmologia, ICOF, Sabino Arana n\u00ba1, 08028 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4094-6346","authenticated-orcid":false,"given":"Marta","family":"Pazos","sequence":"additional","affiliation":[{"name":"Hospital Cl\u00ednic de Barcelona, Institut Clinic d\u2019Oftalmologia, ICOF, Sabino Arana n\u00ba1, 08028 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6154-8323","authenticated-orcid":false,"given":"Xavier","family":"Gasull","sequence":"additional","affiliation":[{"name":"Neurophysiology Laboratory, Department of Biomedicine, Medical School, University of Barcelona, Casanova 143, 08036 Barcelona, Spain"},{"name":"Institute of Neurosciences, University of Barcelona, Edifici de Ponent, 2n vag\u00f3 3r pis, Passeig de la Vall d\u2019Hebron 171, 08035 Barcelona, Spain"},{"name":"Institut d\u2019Investigacions Biom\u00e8diques August Pi i Sunyer (IDIBAPS), Rossell\u00f3 149, 08036 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5572-7512","authenticated-orcid":false,"given":"N\u00faria","family":"Comes","sequence":"additional","affiliation":[{"name":"Neurophysiology Laboratory, Department of Biomedicine, Medical School, University of Barcelona, Casanova 143, 08036 Barcelona, Spain"},{"name":"Institute of Neurosciences, University of Barcelona, Edifici de Ponent, 2n vag\u00f3 3r pis, Passeig de la Vall d\u2019Hebron 171, 08035 Barcelona, Spain"},{"name":"Institut d\u2019Investigacions Biom\u00e8diques August Pi i Sunyer (IDIBAPS), Rossell\u00f3 149, 08036 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,7,26]]},"reference":[{"key":"ref_1","unstructured":"Williams, W. 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