{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T15:05:42Z","timestamp":1783695942479,"version":"3.55.0"},"reference-count":80,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2023,7,26]],"date-time":"2023-07-26T00:00:00Z","timestamp":1690329600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000273","name":"Diabetes Research and Wellness Foundation UK","doi-asserted-by":"publisher","award":["Open funding 2011"],"award-info":[{"award-number":["Open funding 2011"]}],"id":[{"id":"10.13039\/501100000273","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Diabetes Research Fund, Queen Elizabeth Hospital, Gateshead, UK","award":["Open funding 2011"],"award-info":[{"award-number":["Open funding 2011"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Colony forming unit\u2013Hill (CFU\u2013Hill) colonies were established to serve as a sensitive biomarker for vascular health. In animals, the overexpression of miR-7-5p was shown to be pro-atherogenic and associated with increased cardiovascular disease (CVD) risk. In a MERIT study, we aimed to explore the role of miR-7-5p expression in CFU\u2013Hill colonies in type 1 diabetes mellitus (T1DM) and the effect of metformin in subclinical CVD. The expression of miR-7-5p in CFU\u2013Hill colonies in 29 T1DM subjects without CVD and 20 healthy controls (HC) was measured. Metformin was administered to T1DM subjects for eight weeks. MiR-7-5p was upregulated in T1DM whereas metformin reduced it to HC levels. MiR-7-5p was positively correlated with c-reactive protein, and C-X-C motif chemokine ligand 10. The receiver operating characteristic curve revealed miR-7-5p as a biomarker of CVD, and upregulated miR-7-5p, defining subclinical CVD at a HbA1c level of 44.3 mmol\/mol. Ingenuity pathway analysis predicted miR-7-5p to inhibit the mRNA expression of Kr\u00fcppel-like factor 4, epidermal growth factor receptor, insulin-like growth factor 1 receptor, v-raf-1 murine leukemia viral oncogene homolog 1 and insulin receptor substrate \u00bd, and insulin receptor, while metformin activated these miRNAs via transforming growth factor-\u03b21 and Smad2\/3. We proved the pro-atherogenic effect of miR-7-5p that maybe used as a prognostic biomarker.<\/jats:p>","DOI":"10.3390\/ijms241511977","type":"journal-article","created":{"date-parts":[[2023,7,27]],"date-time":"2023-07-27T01:27:46Z","timestamp":1690421266000},"page":"11977","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Decoding of miR-7-5p in Colony Forming Unit\u2013Hill Colonies as a Biomarker of Subclinical Cardiovascular Disease\u2014A MERIT Study"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1580-0409","authenticated-orcid":false,"given":"Sherin","family":"Bakhashab","sequence":"first","affiliation":[{"name":"Biochemistry Department, King Abdulaziz University, Jeddah 21589, Saudi Arabia"},{"name":"Translational and Clinical Research Institute, Newcastle University, Newcastle upon Tyne NE2 4HH, UK"},{"name":"Center of Excellence in Genomic Medicine Research, King Abdulaziz University, Jeddah 2189, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hamzah Pratama","family":"Megantara","sequence":"additional","affiliation":[{"name":"Translational and Clinical Research Institute, Newcastle University, Newcastle upon Tyne NE2 4HH, UK"},{"name":"Faculty of Medicine, Universitas Indonesia, Jakarta 10430, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dimas Kirana","family":"Mahaputra","sequence":"additional","affiliation":[{"name":"Translational and Clinical Research Institute, Newcastle University, Newcastle upon Tyne NE2 4HH, UK"},{"name":"Faculty of Medicine, Universitas Indonesia, Jakarta 10430, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josie","family":"O\u2019Neill","sequence":"additional","affiliation":[{"name":"Translational and Clinical Research Institute, Newcastle University, Newcastle upon Tyne NE2 4HH, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason","family":"Phowira","sequence":"additional","affiliation":[{"name":"Translational and Clinical Research Institute, Newcastle University, Newcastle upon Tyne NE2 4HH, UK"},{"name":"Faculty of Medicine, Universitas Indonesia, Jakarta 10430, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jolanta U.","family":"Weaver","sequence":"additional","affiliation":[{"name":"Translational and Clinical Research Institute, Newcastle University, Newcastle upon Tyne NE2 4HH, UK"},{"name":"Department of Diabetes, Queen Elizabeth Hospital, Gateshead, Newcastle Upon Tyne NE9 6SH, UK"},{"name":"Vascular Biology and Medicine Theme, Newcastle University, Newcastle upon Tyne NE1 7RU, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,7,26]]},"reference":[{"key":"ref_1","unstructured":"(2023). 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