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A metabolomic study of both aqueous and DMSO extracts from Annona muricata leaves recently allowed us to identify dozens of bioactive compounds. In the present study, we use a proteomic approach to detect altered patterns in proteins on both conditioned media and extracts of HT-1080 fibrosarcoma cells under treatment conditions, revealing new potential bioactivities of Annona muricata extracts. Our results reveal the complete sets of deregulated proteins after treatment with aqueous and DMSO extracts from Annona muricata leaves. Functional enrichment analysis of proteomic data suggests deregulation of cell cycle and iron metabolism, which are experimentally validated in vitro. Additional experimental data reveal that DMSO extracts protect HT-1080 fibrosarcoma cells and HMEC-1 endothelial cells from ferroptosis. Data from our proteomic study are available via ProteomeXchange with identifier PXD042354.<\/jats:p>","DOI":"10.3390\/ijms241512021","type":"journal-article","created":{"date-parts":[[2023,7,28]],"date-time":"2023-07-28T01:51:47Z","timestamp":1690509107000},"page":"12021","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["A Proteomic Study of the Bioactivity of Annona muricata Leaf Extracts in HT-1080 Fibrosarcoma Cells"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7308-5912","authenticated-orcid":false,"given":"Ana D\u00e1cil","family":"Marrero","sequence":"first","affiliation":[{"name":"Departamento de Biolog\u00eda Molecular y Bioqu\u00edmica, Facultad de Ciencias, Universidad de M\u00e1laga, Andaluc\u00eda Tech, E-29071 M\u00e1laga, Spain"},{"name":"Instituto de Investigaci\u00f3n Biom\u00e9dica y Plataforma en Nanomedicina-IBIMA Plataforma BIONAND (Biomedical Research Institute of M\u00e1laga), E-29071 M\u00e1laga, Spain"},{"name":"CIBER de Enfermedades Raras (CIBERER), Instituto de Salud Carlos III, E-28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6419-8867","authenticated-orcid":false,"given":"Ana R.","family":"Quesada","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda Molecular y Bioqu\u00edmica, Facultad de Ciencias, Universidad de M\u00e1laga, Andaluc\u00eda Tech, E-29071 M\u00e1laga, Spain"},{"name":"Instituto de Investigaci\u00f3n Biom\u00e9dica y Plataforma en Nanomedicina-IBIMA Plataforma BIONAND (Biomedical Research Institute of M\u00e1laga), E-29071 M\u00e1laga, Spain"},{"name":"CIBER de Enfermedades Raras (CIBERER), Instituto de Salud Carlos III, E-28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3927-3877","authenticated-orcid":false,"given":"Beatriz","family":"Mart\u00ednez-Poveda","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda Molecular y Bioqu\u00edmica, Facultad de Ciencias, Universidad de M\u00e1laga, Andaluc\u00eda Tech, E-29071 M\u00e1laga, Spain"},{"name":"Instituto de Investigaci\u00f3n Biom\u00e9dica y Plataforma en Nanomedicina-IBIMA Plataforma BIONAND (Biomedical Research Institute of M\u00e1laga), E-29071 M\u00e1laga, Spain"},{"name":"CIBER de Enfermedades Cardiovasculares (CIBERCV), Instituto de Salud Carlos III, E-28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7275-6462","authenticated-orcid":false,"given":"Miguel \u00c1ngel","family":"Medina","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda Molecular y Bioqu\u00edmica, Facultad de Ciencias, Universidad de M\u00e1laga, Andaluc\u00eda Tech, E-29071 M\u00e1laga, Spain"},{"name":"Instituto de Investigaci\u00f3n Biom\u00e9dica y Plataforma en Nanomedicina-IBIMA Plataforma BIONAND (Biomedical Research Institute of M\u00e1laga), E-29071 M\u00e1laga, Spain"},{"name":"CIBER de Enfermedades Raras (CIBERER), Instituto de Salud Carlos III, E-28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6259-8296","authenticated-orcid":false,"given":"Casimiro","family":"C\u00e1rdenas","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda Molecular y Bioqu\u00edmica, Facultad de Ciencias, Universidad de M\u00e1laga, Andaluc\u00eda Tech, E-29071 M\u00e1laga, Spain"},{"name":"Research Support Central Services (SCAI), University of M\u00e1laga, E-29071 M\u00e1laga, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,7,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"15625","DOI":"10.3390\/ijms160715625","article-title":"Annona muricata (Annonaceae): A Review of Its Traditional Uses, Isolated Acetogenins and Biological Activities","volume":"16","author":"Moghadamtousi","year":"2015","journal-title":"Int. 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