{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T15:08:16Z","timestamp":1784128096974,"version":"3.55.0"},"reference-count":75,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2023,7,28]],"date-time":"2023-07-28T00:00:00Z","timestamp":1690502400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100010621","name":"Medical University of Lublin","doi-asserted-by":"publisher","award":["PBsd40 (registration number GW\/PB\/13\/2021)"],"award-info":[{"award-number":["PBsd40 (registration number GW\/PB\/13\/2021)"]}],"id":[{"id":"10.13039\/501100010621","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010621","name":"Medical University of Lublin","doi-asserted-by":"publisher","award":["DS43 to A.B.-K."],"award-info":[{"award-number":["DS43 to A.B.-K."]}],"id":[{"id":"10.13039\/501100010621","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Abdominal aortic aneurysm (AAA) is a chronic vascular disease caused by localized weakening and broadening of the abdominal aorta. AAA is a clearly underdiagnosed disease and is burdened with a high mortality rate (65\u201385%) from AAA rupture. Studies indicate that abnormal regulation of angiogenesis and inflammation contributes to progression and onset of this disease; however, dysregulations in the molecular pathways associated with this disease are not yet fully explained. Therefore, in our study, we aimed to identify dysregulations in the key regulators of angiogenesis and inflammation in patients with AAA in peripheral blood mononuclear cells (using qPCR) and plasma samples (using ELISA). Expression levels of ANGPT1, CXCL8, PDGFA, TGFB1, VEGFB, and VEGFC and plasma levels of TGF-alpha, TGF-beta 1, VEGF-A, and VEGF-C were found to be significantly altered in the AAA group compared to the control subjects without AAA. Associations between analyzed factors and risk factors or biochemical parameters were also explored. Any of the analyzed factors was associated with the size of the aneurysm. The presented study identified dysregulations in key angiogenesis- and inflammation-related factors potentially involved in AAA formation, giving new insight into the molecular pathways involved in the development of this disease and providing candidates for biomarkers that could serve as diagnostic or therapeutic targets.<\/jats:p>","DOI":"10.3390\/ijms241512087","type":"journal-article","created":{"date-parts":[[2023,7,28]],"date-time":"2023-07-28T07:35:24Z","timestamp":1690529724000},"page":"12087","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Dysregulations of Key Regulators of Angiogenesis and Inflammation in Abdominal Aortic Aneurysm"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2254-2009","authenticated-orcid":false,"given":"Daniel","family":"Zalewski","sequence":"first","affiliation":[{"name":"Chair and Department of Biology and Genetics, Medical University of Lublin, 4a Chod\u017aki St., 20-093 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8185-4088","authenticated-orcid":false,"given":"Paulina","family":"Chmiel","sequence":"additional","affiliation":[{"name":"Chair and Department of Biology and Genetics, Medical University of Lublin, 4a Chod\u017aki St., 20-093 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3710-0860","authenticated-orcid":false,"given":"Przemys\u0142aw","family":"Ko\u0142odziej","sequence":"additional","affiliation":[{"name":"Chair and Department of Biology and Genetics, Medical University of Lublin, 4a Chod\u017aki St., 20-093 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Grzegorz","family":"Borowski","sequence":"additional","affiliation":[{"name":"Chair and Department of Vascular Surgery and Angiology, Medical University of Lublin, 11 Staszica St., 20-081 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcin","family":"Feldo","sequence":"additional","affiliation":[{"name":"Chair and Department of Vascular Surgery and Angiology, Medical University of Lublin, 11 Staszica St., 20-081 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9541-6353","authenticated-orcid":false,"given":"Janusz","family":"Kocki","sequence":"additional","affiliation":[{"name":"Department of Clinical Genetics, Chair of Medical Genetics, Medical University of Lublin, 11 Radziwi\u0142\u0142owska St., 20-080 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8473-7429","authenticated-orcid":false,"given":"Anna","family":"Bogucka-Kocka","sequence":"additional","affiliation":[{"name":"Chair and Department of Biology and Genetics, Medical University of Lublin, 4a Chod\u017aki St., 20-093 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,7,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Kessler, V., Klopf, J., Eilenberg, W., Neumayer, C., and Brostjan, C. (2022). AAA Revisited: A Comprehensive Review of Risk Factors, Management, and Hallmarks of Pathogenesis. Biomedicines, 10.","DOI":"10.3390\/biomedicines10010094"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1038\/s41572-018-0030-7","article-title":"Abdominal Aortic Aneurysms","volume":"4","author":"Sakalihasan","year":"2018","journal-title":"Nat. Rev. Dis. Primers"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"2101","DOI":"10.1056\/NEJMcp1401430","article-title":"Abdominal Aortic Aneurysms","volume":"371","author":"Kent","year":"2014","journal-title":"N. Engl. J. Med."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Li, X., Zhao, G., Zhang, J., Duan, Z., and Xin, S. (2013). Prevalence and Trends of the Abdominal Aortic Aneurysms Epidemic in General Population\u2014A Meta-Analysis. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0081260"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1634","DOI":"10.1002\/bjs.10200","article-title":"Population-Based Study of Ruptured Abdominal Aortic Aneurysm","volume":"103","author":"Laine","year":"2016","journal-title":"Br. J. Surg."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"2482","DOI":"10.1016\/S0140-6736(17)30639-6","article-title":"SWAN collaborators Morphological Suitability for Endovascular Repair, Non-Intervention Rates, and Operative Mortality in Women and Men Assessed for Intact Abdominal Aortic Aneurysm Repair: Systematic Reviews with Meta-Analysis","volume":"389","author":"Ulug","year":"2017","journal-title":"Lancet"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1002\/bjs.10715","article-title":"Gloucestershire and Swindon Abdominal Aortic Aneurysm Screening Programme Lessons Learned about Prevalence and Growth Rates of Abdominal Aortic Aneurysms from a 25-Year Ultrasound Population Screening Programme","volume":"105","author":"Sweeting","year":"2018","journal-title":"Br. J. Surg."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1016\/j.ejvs.2012.12.013","article-title":"International Variations in AAA Screening","volume":"45","author":"Stather","year":"2013","journal-title":"Eur. J. Vasc. Endovasc. Surg."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1032","DOI":"10.1007\/s00261-017-1450-7","article-title":"Epidemiology and Contemporary Management of Abdominal Aortic Aneurysms","volume":"43","author":"Ullery","year":"2018","journal-title":"Abdom. Radiol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1016\/j.jvs.2017.10.044","article-title":"The Society for Vascular Surgery Practice Guidelines on the Care of Patients with an Abdominal Aortic Aneurysm","volume":"67","author":"Chaikof","year":"2018","journal-title":"J. Vasc. Surg."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Maguire, E.M., Pearce, S.W.A., Xiao, R., Oo, A.Y., and Xiao, Q. (2019). Matrix Metalloproteinase in Abdominal Aortic Aneurysm and Aortic Dissection. Pharmaceuticals, 12.","DOI":"10.3390\/ph12030118"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1038\/nrcardio.2010.180","article-title":"Pathophysiology and Epidemiology of Abdominal Aortic Aneurysms","volume":"8","author":"Nordon","year":"2011","journal-title":"Nat. Rev. Cardiol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1038\/s41569-018-0114-9","article-title":"Abdominal Aortic Aneurysm: Update on Pathogenesis and Medical Treatments","volume":"16","author":"Golledge","year":"2019","journal-title":"Nat. Rev. Cardiol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"609161","DOI":"10.3389\/fimmu.2020.609161","article-title":"Abdominal Aortic Aneurysm: Roles of Inflammatory Cells","volume":"11","author":"Yuan","year":"2021","journal-title":"Front. Immunol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1038\/nrcardio.2017.52","article-title":"Monocytes and Macrophages in Abdominal Aortic Aneurysm","volume":"14","author":"Raffort","year":"2017","journal-title":"Nat. Rev. Cardiol."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Sano, M., Sasaki, T., Hirakawa, S., Sakabe, J., Ogawa, M., Baba, S., Zaima, N., Tanaka, H., Inuzuka, K., and Yamamoto, N. (2014). Lymphangiogenesis and Angiogenesis in Abdominal Aortic Aneurysm. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0089830"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1196\/annals.1383.007","article-title":"Increased Angiogenesis at the Site of Abdominal Aortic Aneurysm Rupture","volume":"1085","author":"Choke","year":"2006","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2077","DOI":"10.1161\/01.ATV.0000234944.22509.f9","article-title":"Abdominal Aortic Aneurysm Rupture Is Associated With Increased Medial Neovascularization and Overexpression of Proangiogenic Cytokines","volume":"26","author":"Choke","year":"2006","journal-title":"Arterioscler. Thromb. Vasc. Biol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"758","DOI":"10.1111\/eci.12103","article-title":"Potential Role for Anti-Angiogenic Therapy in Abdominal Aortic Aneurysms","volume":"43","author":"Vijaynagar","year":"2013","journal-title":"Eur. J. Clin. Investig."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1006\/jsre.2002.6468","article-title":"Expression of Angiogenesis and Angiogenic Factors in Human Aortic Vascular Disease","volume":"106","author":"Kobayashi","year":"2002","journal-title":"J. Surg. Res."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1161\/CIRCRESAHA.118.312438","article-title":"Role of Noncoding RNAs in the Pathogenesis of Abdominal Aortic Aneurysm","volume":"124","author":"Kumar","year":"2019","journal-title":"Circ. Res."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1016\/j.atherosclerosis.2017.03.010","article-title":"A Systematic Review Investigating the Association of MicroRNAs with Human Abdominal Aortic Aneurysms","volume":"261","author":"Iyer","year":"2017","journal-title":"Atherosclerosis"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"2382","DOI":"10.1161\/CIRCULATIONAHA.108.802074","article-title":"Circulating Markers of Abdominal Aortic Aneurysm Presence and Progression","volume":"118","author":"Golledge","year":"2008","journal-title":"Circulation"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"925840","DOI":"10.1155\/2014\/925840","article-title":"Novel Biomarkers of Abdominal Aortic Aneurysm Disease: Identifying Gaps and Dispelling Misperceptions","volume":"2014","author":"Moris","year":"2014","journal-title":"BioMed Res. Int."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"19","DOI":"10.5603\/AA.2019.0004","article-title":"The Review of Selected Biomarkers of Abdominal Aortic Aneurysm","volume":"25","author":"Surma","year":"2019","journal-title":"Acta Angiol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1016\/j.ejvs.2008.05.009","article-title":"Potential Circulating Biomarkers for Abdominal Aortic Aneurysm Expansion and Rupture\u2014A Systematic Review","volume":"36","author":"Urbonavicius","year":"2008","journal-title":"Eur. J. Vasc. Endovasc. Surg."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1153899","DOI":"10.3389\/fgene.2023.1153899","article-title":"The Role of Long Non-Coding RNA in Abdominal Aortic Aneurysm","volume":"14","author":"Xu","year":"2023","journal-title":"Front. Genet."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Zalewski, D.P., Ruszel, K.P., St\u0119pniewski, A., Ga\u0142kowski, D., Bogucki, J., Komsta, \u0141., Ko\u0142odziej, P., Chmiel, P., Zubilewicz, T., and Feldo, M. (2020). Dysregulation of MicroRNA Modulatory Network in Abdominal Aortic Aneurysm. J. Clin. Med., 9.","DOI":"10.3390\/jcm9061974"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Gurung, R., Choong, A.M., Woo, C.C., Foo, R., and Sorokin, V. (2020). Genetic and Epigenetic Mechanisms Underlying Vascular Smooth Muscle Cell Phenotypic Modulation in Abdominal Aortic Aneurysm. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21176334"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"11772719221095676","DOI":"10.1177\/11772719221095676","article-title":"Cytokines in Abdominal Aortic Aneurysm: Master Regulators With Clinical Application","volume":"17","author":"Puchenkova","year":"2022","journal-title":"Biomark. Insights"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1016\/j.atherosclerosis.2010.08.051","article-title":"Angiopoietins, Abdominal Aortic Aneurysm and Atherosclerosis","volume":"214","author":"Trollope","year":"2011","journal-title":"Atherosclerosis"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1016\/j.avsg.2017.10.007","article-title":"Overexpression of Angiopoietin-1 Potentiates Endothelial Progenitor Cells for the Treatment of Aneurysm","volume":"48","author":"Lu","year":"2018","journal-title":"Ann. Vasc. Surg."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1016\/j.jns.2015.05.038","article-title":"High Angiopoietin-1 Levels Predict a Good Functional Outcome within 72h of an Aneurysmal Subarachnoid Hemorrhage: A Prospective Study from a Single Center","volume":"356","author":"Wang","year":"2015","journal-title":"J. Neurol. Sci."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1016\/j.cyto.2018.03.031","article-title":"CXCL8 Hyper-Signaling in the Aortic Abdominal Aneurysm","volume":"108","author":"Kokje","year":"2018","journal-title":"Cytokine"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/j.ejvs.2008.09.013","article-title":"Characterisation of Interleukin-8 and Monocyte Chemoattractant Protein-1 Expression within the Abdominal Aortic Aneurysm and Their Association with Mural Inflammation","volume":"37","author":"Middleton","year":"2009","journal-title":"Eur. J. Vasc. Endovasc. Surg."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Kami\u0144ska, J., Lyson, T., Chrzanowski, R., Sawicki, K., Milewska, A.J., Tylicka, M., Zi\u0144czuk, J., Matowicka-Karna, J., Dymicka-Piekarska, V., and Mariak, Z. (2020). Ratio of IL-8 in CSF Versus Serum Is Elevated in Patients with Unruptured Brain Aneurysm. J. Clin. Med., 9.","DOI":"10.3390\/jcm9061761"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"2594","DOI":"10.1161\/STROKEAHA.113.002361","article-title":"Localized Increase of Chemokines in the Lumen of Human Cerebral Aneurysms","volume":"44","author":"Chalouhi","year":"2013","journal-title":"Stroke"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1016\/j.ijbiomac.2022.01.113","article-title":"Targeting the PDGF\/PDGFR Signaling Pathway for Cancer Therapy: A Review","volume":"202","author":"Zou","year":"2022","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"669188","DOI":"10.3389\/fcell.2021.669188","article-title":"Role of PDGF-A\/B Ligands in Cardiac Repair After Myocardial Infarction","volume":"9","author":"Kalra","year":"2021","journal-title":"Front. Cell Dev. Biol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1067\/mva.2001.113310","article-title":"Simultaneous Analysis of 1176 Gene Products in Normal Human Aorta and Abdominal Aortic Aneurysms Using a Membrane-Based Complementary DNA Expression Array","volume":"34","author":"Tung","year":"2001","journal-title":"J. Vasc. Surg."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Wan, L., Huang, J., Ni, H., and Yu, G. (2018). Screening Key Genes for Abdominal Aortic Aneurysm Based on Gene Expression Omnibus Dataset. BMC Cardiovasc. Disord., 18.","DOI":"10.1186\/s12872-018-0766-8"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"830","DOI":"10.1177\/0003319719844678","article-title":"A Pilot Study of Protein Microarray for Simultaneous Analysis of 274 Cytokines Between Abdominal Aortic Aneurysm and Normal Aorta","volume":"70","author":"Yuwen","year":"2019","journal-title":"Angiology"},{"key":"ref_43","first-page":"271","article-title":"The Expression of Platelet-Derived Growth Factor and Connective Tissue Growth Factor in Different Types of Abdominal Aortic Aneurysms","volume":"46","author":"Kanazawa","year":"2005","journal-title":"J. Cardiovasc. Surg."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Sun, W., Zheng, J., and Gao, Y. (2022). Targeting Platelet Activation in Abdominal Aortic Aneurysm: Current Knowledge and Perspectives. Biomolecules, 12.","DOI":"10.3390\/biom12020206"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"888563","DOI":"10.3389\/fphar.2022.888563","article-title":"Association of TGF-\u03b2 Canonical Signaling-Related Core Genes With Aortic Aneurysms and Aortic Dissections","volume":"13","author":"Chen","year":"2022","journal-title":"Front. Pharmacol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1016\/j.carpath.2012.07.005","article-title":"Transforming Growth Factor-\u03b2 and Abdominal Aortic Aneurysms","volume":"22","author":"Wang","year":"2013","journal-title":"Cardiovasc. Pathol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1983","DOI":"10.1161\/ATVBAHA.117.310031","article-title":"Further Evidence Supporting a Protective Role of Transforming Growth Factor-\u03b2 (TGF\u03b2) in Aortic Aneurysm and Dissection","volume":"37","author":"Tellides","year":"2017","journal-title":"Arterioscler. Thromb. Vasc. Biol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"388","DOI":"10.1161\/CIRCRESAHA.115.308040","article-title":"Smad4 Deficiency in Smooth Muscle Cells Initiates the Formation of Aortic Aneurysm","volume":"118","author":"Zhang","year":"2016","journal-title":"Circ. Res."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"2102","DOI":"10.1161\/ATVBAHA.117.309401","article-title":"TGF-\u03b2 (Transforming Growth Factor-\u03b2) Signaling Protects the Thoracic and Abdominal Aorta From Angiotensin II-Induced Pathology by Distinct Mechanisms","volume":"37","author":"Angelov","year":"2017","journal-title":"Arterioscler. Thromb. Vasc. Biol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"358","DOI":"10.1126\/science.1192149","article-title":"Noncanonical TGF\u03b2 Signaling Contributes to Aortic Aneurysm Progression in Marfan Syndrome Mice","volume":"332","author":"Holm","year":"2011","journal-title":"Science"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1721727X221091532","DOI":"10.1177\/1721727X221091532","article-title":"Daxx Ameliorates Abdominal Aortic Aneurysm through Inhibiting the TGF-\u03921-Mediated PI3K\/AKT\/ID2 Signaling Pathway","volume":"20","author":"Chen","year":"2022","journal-title":"Eur. J. Inflamm."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1016\/j.cca.2019.10.042","article-title":"The TGF-\u03b2 Pathway Plays a Key Role in Aortic Aneurysms","volume":"501","author":"Tingting","year":"2020","journal-title":"Clin. Chim. Acta"},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Sepetiene, R., Patamsyte, V., Zukovas, G., Jariene, G., Stanioniene, Z., Benetis, R., and Lesauskaite, V. (2015). Blood Plasma TGF- \u03921 Concentration in Sporadic Dilatative Pathology of Ascending Aorta: More Questions than Answers. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0129353"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1093\/icvts\/ivx153","article-title":"Increased Blood Levels of Transforming Growth Factor \u03b2 in Patients with Aortic Dilatation","volume":"25","author":"Lamas","year":"2017","journal-title":"Interact. Cardiovasc. Thorac. Surg."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"55","DOI":"10.2147\/TACG.S96233","article-title":"Marfan Syndrome: Current Perspectives","volume":"9","author":"Pepe","year":"2016","journal-title":"Appl. Clin. Genet."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"16872","DOI":"10.1038\/srep16872","article-title":"Fibulin-4 Deficiency Increases TGF-\u03b2 Signalling in Aortic Smooth Muscle Cells Due to Elevated TGF-\u03922 Levels","volume":"5","author":"Ramnath","year":"2015","journal-title":"Sci. Rep."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"526","DOI":"10.1161\/CIRCULATIONAHA.108.841981","article-title":"Circulating Transforming Growth Factor-\u03b2 in Marfan Syndrome","volume":"120","author":"Matt","year":"2009","journal-title":"Circulation"},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Wang, C., Lv, X., Jiang, C., Cordes, C.M., Fu, L., Lele, S.M., and Davis, J.S. (2012). Transforming Growth Factor Alpha (TGF\u03b1) Regulates Granulosa Cell Tumor (GCT) Cell Proliferation and Migration through Activation of Multiple Pathways. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0048299"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"901","DOI":"10.1016\/j.bbrc.2018.10.137","article-title":"Transforming Growth Factor Alpha Promotes Tumorigenesis and Regulates Epithelial-Mesenchymal Transition Modulation in Colon Cancer","volume":"506","author":"Yu","year":"2018","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1016\/j.bcp.2014.03.010","article-title":"Transforming Growth Factor Alpha Promotes Osteosarcoma Metastasis by ICAM-1 and PI3K\/Akt Signaling Pathway","volume":"89","author":"Hou","year":"2014","journal-title":"Biochem. Pharmacol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"358","DOI":"10.1093\/cvr\/cvr080","article-title":"Role of Vascular Endothelial Growth Factor-A in Development of Abdominal Aortic Aneurysm","volume":"91","author":"Kaneko","year":"2011","journal-title":"Cardiovasc. Res."},{"key":"ref_62","doi-asserted-by":"crossref","unstructured":"Li, J., Krishna, S.M., and Golledge, J. (2016). The Potential Role of Kallistatin in the Development of Abdominal Aortic Aneurysm. Int. J. Mol. Sci., 17.","DOI":"10.3390\/ijms17081312"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"1652","DOI":"10.1161\/ATVBAHA.119.312497","article-title":"Inhibition of VEGF-A or Its Receptor Activity Suppresses Experimental Aneurysm Progression in the Aortic Elastase Infusion Model","volume":"39","author":"Xu","year":"2019","journal-title":"Arterioscler. Thromb. Vasc. Biol."},{"key":"ref_64","first-page":"7","article-title":"Usefulness of Serum VEGF Concentration Measurement to Estimate Aortic Aneurysm Risk of Rupture","volume":"12","author":"Kunecki","year":"2006","journal-title":"Acta Angiol."},{"key":"ref_65","doi-asserted-by":"crossref","unstructured":"Iwa\u0144czyk, S., Lehmann, T., Cie\u015blewicz, A., Radziemski, A., Malesza, K., Wroty\u0144ski, M., Jagodzi\u0144ski, P.P., Grygier, M., Lesiak, M., and Araszkiewicz, A. (2021). Involvement of Angiogenesis in the Pathogenesis of Coronary Aneurysms. Biomedicines, 9.","DOI":"10.3390\/biomedicines9091269"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"596","DOI":"10.1203\/00006450-199810000-00021","article-title":"Increased Serum Levels of Vascular Endothelial Growth Factor in Kawasaki Disease","volume":"44","author":"Maeno","year":"1998","journal-title":"Pediatr. Res."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1007\/s10143-005-0411-8","article-title":"VEGF Plasma Levels in Nonruptured Intracranial Aneurysms","volume":"29","author":"Sandalcioglu","year":"2006","journal-title":"Neurosurg. Rev."},{"key":"ref_68","first-page":"520","article-title":"Expression of VEGFs and Its Receptors in Abdominal Aortic Aneurysm","volume":"34","author":"Wolanska","year":"2015","journal-title":"Int. Angiol."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"1248","DOI":"10.1016\/j.cell.2019.01.021","article-title":"VEGF in Signaling and Disease: Beyond Discovery and Development","volume":"176","author":"Apte","year":"2019","journal-title":"Cell"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1038\/nrd.2015.17","article-title":"Ten Years of Anti-Vascular Endothelial Growth Factor Therapy","volume":"15","author":"Ferrara","year":"2016","journal-title":"Nat. Rev. Drug Discov."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"1938","DOI":"10.1002\/jcb.30344","article-title":"Molecular Mechanism of VEGF and Its Role in Pathological Angiogenesis","volume":"123","author":"Ahmad","year":"2022","journal-title":"J. Cell. Biochem."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"1200","DOI":"10.3389\/fgene.2019.01200","article-title":"Dysregulation of MicroRNA Regulatory Network in Lower Extremities Arterial Disease","volume":"10","author":"Zalewski","year":"2019","journal-title":"Front. Genet."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2(-Delta Delta C(T)) Method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"1101","DOI":"10.1038\/nprot.2008.73","article-title":"Analyzing Real-Time PCR Data by the Comparative C(T) Method","volume":"3","author":"Schmittgen","year":"2008","journal-title":"Nat. Protoc."},{"key":"ref_75","doi-asserted-by":"crossref","unstructured":"Robin, X., Turck, N., Hainard, A., Tiberti, N., Lisacek, F., Sanchez, J.-C., and M\u00fcller, M. (2011). PROC: An Open-Source Package for R and S+ to Analyze and Compare ROC Curves. BMC Bioinform., 12.","DOI":"10.1186\/1471-2105-12-77"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/15\/12087\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T20:21:20Z","timestamp":1760127680000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/15\/12087"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,7,28]]},"references-count":75,"journal-issue":{"issue":"15","published-online":{"date-parts":[[2023,8]]}},"alternative-id":["ijms241512087"],"URL":"https:\/\/doi.org\/10.3390\/ijms241512087","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,7,28]]}}}