{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T02:25:28Z","timestamp":1760149528498,"version":"build-2065373602"},"reference-count":58,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2023,7,31]],"date-time":"2023-07-31T00:00:00Z","timestamp":1690761600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"\u201cCenter of Photonics\u201d funded by the Ministry of Science and Higher Education of the Russian Federation","award":["075-15-2022-293"],"award-info":[{"award-number":["075-15-2022-293"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Astrocytes serve many functions in the brain related to maintaining nerve tissue homeostasis and regulating neuronal function, including synaptic transmission. It is assumed that astrocytes are crucial players in determining the physiological or pathological outcome of the brain aging process and the development of neurodegenerative diseases. Therefore, studies on the peculiarities of astrocyte physiology and interastrocytic signaling during aging are of utmost importance. Calcium waves are one of the main mechanisms of signal transmission between astrocytes, and in the present study we investigated the features of calcium dynamics in primary cultures of murine cortical astrocytes in physiological aging and hypoxia modeling in vitro. Specifically, we focused on the assessment of calcium network dynamics and the restructuring of the functional network architecture in primary astrocytic cultures. Calcium imaging was performed on days 21 (\u201cyoung\u201d astrocyte group) and 150 (\u201cold\u201d astrocyte group) of cultures\u2019 development in vitro. While the number of active cells and frequency of calcium events were decreased, we observed a reduced degree of correlation in calcium dynamics between neighboring cells, which was accompanied by a reduced number of functionally connected cells with fewer and slower signaling events. At the same time, an increase in the mRNA expression of anti-apoptotic factor Bcl-2 and connexin 43 was observed in \u201cold\u201d astrocytic cultures, which can be considered as a compensatory response of cells with a decreased level of intercellular communication. A hypoxic episode aggravates the depression of the connectivity of calcium dynamics of \u201cyoung\u201d astrocytes rather than that of \u201cold\u201d ones.<\/jats:p>","DOI":"10.3390\/ijms241512286","type":"journal-article","created":{"date-parts":[[2023,8,1]],"date-time":"2023-08-01T09:06:44Z","timestamp":1690880804000},"page":"12286","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Unravelling the Collective Calcium Dynamics of Physiologically Aged Astrocytes under a Hypoxic State In Vitro"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1703-2618","authenticated-orcid":false,"given":"Elena V.","family":"Mitroshina","sequence":"first","affiliation":[{"name":"Institute of Biology and Biomedicine, Lobachevsky State University of Nizhny Novgorod, 23 Gagarin Avenue, 603022 Nizhny Novgorod, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1169-5149","authenticated-orcid":false,"given":"Mikhail I.","family":"Krivonosov","sequence":"additional","affiliation":[{"name":"Institute of Biology and Biomedicine, Lobachevsky State University of Nizhny Novgorod, 23 Gagarin Avenue, 603022 Nizhny Novgorod, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander M.","family":"Pakhomov","sequence":"additional","affiliation":[{"name":"Institute of Biology and Biomedicine, Lobachevsky State University of Nizhny Novgorod, 23 Gagarin Avenue, 603022 Nizhny Novgorod, Russia"},{"name":"Federal Research Center Institute of Applied Physics of the Russian Academy of Sciences (IAP RAS), 603950 Nizhny Novgorod, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laysan E.","family":"Yarullina","sequence":"additional","affiliation":[{"name":"Institute of Biology and Biomedicine, Lobachevsky State University of Nizhny Novgorod, 23 Gagarin Avenue, 603022 Nizhny Novgorod, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria S.","family":"Gavrish","sequence":"additional","affiliation":[{"name":"Institute of Biology and Biomedicine, Lobachevsky State University of Nizhny Novgorod, 23 Gagarin Avenue, 603022 Nizhny Novgorod, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4463-5035","authenticated-orcid":false,"given":"Tatiana A.","family":"Mishchenko","sequence":"additional","affiliation":[{"name":"Institute of Biology and Biomedicine, Lobachevsky State University of Nizhny Novgorod, 23 Gagarin Avenue, 603022 Nizhny Novgorod, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roman S.","family":"Yarkov","sequence":"additional","affiliation":[{"name":"Institute of Biology and Biomedicine, Lobachevsky State University of Nizhny Novgorod, 23 Gagarin Avenue, 603022 Nizhny Novgorod, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9759-6477","authenticated-orcid":false,"given":"Maria V.","family":"Vedunova","sequence":"additional","affiliation":[{"name":"Institute of Biology and Biomedicine, Lobachevsky State University of Nizhny Novgorod, 23 Gagarin Avenue, 603022 Nizhny Novgorod, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,7,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"a035188","DOI":"10.1101\/cshperspect.a035188","article-title":"Astroglial Calcium Signaling in Aging and Alzheimer\u2019s Disease","volume":"11","author":"Verkhratsky","year":"2019","journal-title":"Cold Spring Harb. Perspect. Biol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"101775","DOI":"10.1016\/j.arr.2022.101775","article-title":"An Emerging Role of Astrocytes in Aging\/Neuroinflammation and Gut-Brain Axis with Consequences on Sleep and Sleep Disorders","volume":"83","author":"Gudkov","year":"2023","journal-title":"Ageing Res. Rev."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1007\/s00401-009-0556-6","article-title":"Dystrophic (Senescent) Rather than Activated Microglial Cells Are Associated with Tau Pathology and Likely Precede Neurodegeneration in Alzheimer\u2019s Disease","volume":"118","author":"Streit","year":"2009","journal-title":"Acta Neuropathol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"ra8","DOI":"10.1126\/scisignal.2002160","article-title":"Extracellular Ca2+ Acts as a Mediator of Communication from Neurons to Glia","volume":"5","author":"Torres","year":"2012","journal-title":"Sci. Signal"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"20849","DOI":"10.1074\/jbc.R116.735894","article-title":"Why Calcium? How Calcium Became the Best Communicator","volume":"291","author":"Carafoli","year":"2016","journal-title":"J. Biol. Chem."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"716","DOI":"10.1002\/glia.20374","article-title":"Astrocyte Calcium Waves: What They Are and What They Do","volume":"54","author":"Scemes","year":"2006","journal-title":"Glia"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"AN20110061","DOI":"10.1042\/AN20110061","article-title":"Astroglial Excitability and Gliotransmission: An Appraisal of Ca2+ as a Signalling Route","volume":"4","author":"Zorec","year":"2012","journal-title":"ASN Neuro"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"2607","DOI":"10.1523\/JNEUROSCI.5319-10.2011","article-title":"Large-Scale Calcium Waves Traveling through Astrocytic Networks In Vivo","volume":"31","author":"Kuga","year":"2011","journal-title":"J. Neurosci."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"4926","DOI":"10.1523\/JNEUROSCI.1988-22.2023","article-title":"Neuronally-Derived Soluble Abeta Evokes Cell-Wide Astrocytic Calcium Dysregulation in Absence of Amyloid Plaques In Vivo","volume":"43","author":"Kelly","year":"2023","journal-title":"J. Neurosci."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Mitroshina, E.V., Krivonosov, M.I., Burmistrov, D.E., Savyuk, M.O., Mishchenko, T.A., Ivanchenko, M.V., and Vedunova, M.V. (2020). Signatures of the Consolidated Response of Astrocytes to Ischemic Factors In Vitro. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21217952"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Mitroshina, E.V., Pakhomov, A.M., Krivonosov, M.I., Yarkov, R.S., Gavrish, M.S., Shkirin, A.V., Ivanchenko, M.V., and Vedunova, M.V. (2022). Novel Algorithm of Network Calcium Dynamics Analysis for Studying the Role of Astrocytes in Neuronal Activity in Alzheimer\u2019s Disease Models. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms232415928"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1016\/0197-4580(87)90073-X","article-title":"Hypothesis on the Regulation of Cytosol Calcium Concentration and the Aging Brain","volume":"8","author":"Khachaturian","year":"1987","journal-title":"Neurobiol. Aging"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1016\/S0166-2236(97)01156-9","article-title":"Calcium and Neuronal Ageing","volume":"21","author":"Verkhratsky","year":"1998","journal-title":"Trends Neurosci."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"546","DOI":"10.1177\/1073858407299730","article-title":"The Pathogenesis of Alzheimers Disease\u2014Is It a Lifelong \u201cCalciumopathy\u201d?","volume":"13","author":"Stutzmann","year":"2007","journal-title":"Neuroscientist"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"20150429","DOI":"10.1098\/rstb.2015.0429","article-title":"Impairments of Neural Circuit Function in Alzheimer\u2019s Disease","volume":"371","author":"Busche","year":"2016","journal-title":"Philos. Trans. R. Soc. B Biol. Sci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"344","DOI":"10.2174\/1567205017666200528162046","article-title":"Reversal of Calcium Dysregulation as Potential Approach for Treating Alzheimer\u2019s Disease","volume":"17","author":"Popugaeva","year":"2020","journal-title":"Curr. Alzheimer Res."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1111\/j.1474-9726.2007.00296.x","article-title":"The Importance of Being Subtle: Small Changes in Calcium Homeostasis Control Cognitive Decline in Normal Aging","volume":"6","author":"Toescu","year":"2007","journal-title":"Aging Cell"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"614","DOI":"10.1016\/j.tins.2004.07.010","article-title":"Ca2+ Regulation and Gene Expression in Normal Brain Aging","volume":"27","author":"Toescu","year":"2004","journal-title":"Trends Neurosci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"e13334","DOI":"10.1111\/acel.13334","article-title":"Astrocyte Dystrophy in Ageing Brain Parallels Impaired Synaptic Plasticity","volume":"20","author":"Popov","year":"2021","journal-title":"Aging Cell"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"15","DOI":"10.3233\/JAD-170589","article-title":"Hypoxia-Induced Signaling Activation in Neurodegenerative Diseases: Targets for New Therapeutic Strategies","volume":"62","author":"Jha","year":"2018","journal-title":"J. Alzheimer\u2019s Dis."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"779","DOI":"10.1007\/s13770-023-00547-8","article-title":"D-Galactose and Hypoxia Induce the Early Onset of Age-Related Hearing Loss Deterioration in a Mouse Model","volume":"20","author":"Batsaikhan","year":"2023","journal-title":"Tissue Eng. Regen. Med."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"251","DOI":"10.3389\/fnagi.2020.00251","article-title":"Antenatal Hypoxia Accelerates the Onset of Alzheimer\u2019s Disease Pathology in 5xFAD Mouse Model","volume":"12","author":"Shen","year":"2020","journal-title":"Front. Aging Neurosci."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/j.neurobiolaging.2012.06.009","article-title":"Effect of Age on Neocortical Brain Cells in 90+ Year Old Human Females\u2014A Cell Counting Study","volume":"34","author":"Fabricius","year":"2013","journal-title":"Neurobiol. Aging"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"831","DOI":"10.1002\/glia.20967","article-title":"Concomitant Astroglial Atrophy and Astrogliosis in a Triple Transgenic Animal Model of Alzheimer\u2019s Disease","volume":"58","author":"Olabarria","year":"2010","journal-title":"Glia"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1016\/j.bbi.2016.01.006","article-title":"Glial Cell Morphological and Density Changes through the Lifespan of Rhesus Macaques","volume":"55","author":"Robillard","year":"2016","journal-title":"Brain Behav. Immun."},{"key":"ref_26","unstructured":"Verkhratsky, A., Li, B., Scuderi, C., and Parpura, V. (2021). Advances in Neurobiology, Springer."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/0197-4580(91)90056-P","article-title":"Age-Related Changes in Glial Fibrillary Acidic Protein MRNA in the Mouse Brain","volume":"12","author":"Goss","year":"1991","journal-title":"Neurobiol. Aging"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/0197-4580(95)80008-F","article-title":"Increases of Glial Fibrillary Acidic Protein in the Aging Female Mouse Brain","volume":"16","author":"Kohama","year":"1995","journal-title":"Neurobiol. Aging"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1016\/0197-4580(93)90100-P","article-title":"GFAP MRNA Increases with Age in Rat and Human Brain","volume":"14","author":"Nichols","year":"1993","journal-title":"Neurobiol. Aging"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/S0304-3940(97)00708-8","article-title":"Glial Reaction in the Hippocampal Formation Is Highly Correlated with Aging in Human Brain","volume":"235","author":"David","year":"1997","journal-title":"Neurosci. Lett."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Cerbai, F., Lana, D., Nosi, D., Petkova-Kirova, P., Zecchi, S., Brothers, H.M., Wenk, G.L., and Giovannini, M.G. (2012). The Neuron-Astrocyte-Microglia Triad in Normal Brain Ageing and in a Model of Neuroinflammation in the Rat Hippocampus. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0045250"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1007\/s11357-019-00100-3","article-title":"Astrocyte Senescence May Drive Alterations in GFAP\u03b1, CDKN2A P14ARF, and TAU3 Transcript Expression and Contribute to Cognitive Decline","volume":"41","author":"Lye","year":"2019","journal-title":"Geroscience"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"643743","DOI":"10.3389\/fneur.2021.643743","article-title":"One-Year Prospective Study of Plasma Biomarkers from CNS in Patients with Mild Traumatic Brain Injury","volume":"12","author":"Clarke","year":"2021","journal-title":"Front. Neurol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1016\/j.mad.2007.01.005","article-title":"Age-Related Changes of Astorocytes, Oligodendrocytes and Microglia in the Mouse Hippocampal CA1 Sector","volume":"128","author":"Hayakawa","year":"2007","journal-title":"Mech. Ageing Dev."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"208","DOI":"10.3389\/fnagi.2016.00208","article-title":"Changes in the Transcriptome of Human Astrocytes Accompanying Oxidative Stress-Induced Senescence","volume":"8","author":"Crowe","year":"2016","journal-title":"Front. Aging Neurosci."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.neurobiolaging.2013.07.002","article-title":"Complex and Region-Specific Changes in Astroglial Markers in the Aging Brain","volume":"35","author":"Yeh","year":"2014","journal-title":"Neurobiol. Aging"},{"key":"ref_37","first-page":"1764589","article-title":"Neuroprotective Effect of HIIT against GFAP Hypertrophy through Mitochondrial Dynamics in APP\/PS1 Mice","volume":"2022","author":"Liu","year":"2022","journal-title":"Oxid Med. Cell Longev."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"392","DOI":"10.1111\/j.1474-9726.2011.00682.x","article-title":"Age-Dependent Remodelling of Ionotropic Signalling in Cortical Astroglia","volume":"10","author":"Lalo","year":"2011","journal-title":"Aging Cell"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1016\/j.semcdb.2011.02.012","article-title":"Ionotropic ATP Receptors in Neuronal\u2013Glial Communication","volume":"22","author":"Lalo","year":"2011","journal-title":"Semin. Cell Dev. Biol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1002\/glia.23112","article-title":"Neuron-Astrocyte Signaling Is Preserved in the Aging Brain","volume":"65","author":"Mederos","year":"2017","journal-title":"Glia"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"239","DOI":"10.3389\/fnmol.2018.00239","article-title":"Diversity of Astroglial Effects on Aging- and Experience-Related Cortical Metaplasticity","volume":"11","author":"Lalo","year":"2018","journal-title":"Front. Mol. Neurosci."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1016\/j.ceca.2010.09.004","article-title":"Ionotropic NMDA and P2X1\/5 Receptors Mediate Synaptically Induced Ca2+ Signalling in Cortical Astrocytes","volume":"48","author":"Palygin","year":"2010","journal-title":"Cell Calcium"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1275","DOI":"10.1042\/BST20140163","article-title":"Exocytosis of Gliotransmitters from Cortical Astrocytes: Implications for Synaptic Plasticity and Aging","volume":"42","author":"Lalo","year":"2014","journal-title":"Biochem. Soc. Trans."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"101343","DOI":"10.1016\/j.arr.2021.101343","article-title":"Hypoxia and Brain Aging: Neurodegeneration or Neuroprotection?","volume":"68","author":"Burtscher","year":"2021","journal-title":"Ageing Res. Rev."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.neurobiolaging.2022.12.006","article-title":"Capillary Dysfunction Correlates with Cortical Amyloid Load in Early Alzheimer\u2019s Disease","volume":"123","author":"Madsen","year":"2023","journal-title":"Neurobiol. Aging"},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Liu, Y., Tan, J., Miao, Y., and Zhang, Q. (Curr. Stem Cell Res., 2023). Neurogenesis, A Potential Target for Intermittent Hypoxia Leading to Cognitive Decline, Curr. Stem Cell Res., online ahead of print.","DOI":"10.2174\/1574888X18666230330083206"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1016\/j.freeradbiomed.2023.05.011","article-title":"Chronic Hypoxia of Endothelial Cells Boosts HIF-1\u03b1-NLRP1 Circuit in Alzheimer\u2019s Disease","volume":"204","author":"Jung","year":"2023","journal-title":"Free Radic. Biol. Med."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"12991","DOI":"10.1038\/s41598-017-13408-x","article-title":"Uncoupling Oncogene-Induced Senescence (OIS) and DNA Damage Response (DDR) Triggered by DNA Hyper-Replication: Lessons from Primary Mouse Embryo Astrocytes (MEA)","volume":"7","author":"Seoane","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1111\/j.1460-9568.2011.07738.x","article-title":"Astrocytes in the Aging Brain Express Characteristics of Senescence-Associated Secretory Phenotype","volume":"34","author":"Salminen","year":"2011","journal-title":"Eur. J. Neurosci."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Bhat, R., Crowe, E.P., Bitto, A., Moh, M., Katsetos, C.D., Garcia, F.U., Johnson, F.B., Trojanowski, J.Q., Sell, C., and Torres, C. (2012). Astrocyte Senescence as a Component of Alzheimer\u2019s Disease. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0045069"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"264","DOI":"10.22365\/jpsych.2016.274.264","article-title":"Pro-Inflammatory Cytokines in Alzheimer\u2019s Disease","volume":"27","author":"Stamouli","year":"2016","journal-title":"Psychiatriki"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"797","DOI":"10.1007\/s00702-017-1745-4","article-title":"Type-I Interferon Pathway in Neuroinflammation and Neurodegeneration: Focus on Alzheimer\u2019s Disease","volume":"125","author":"Taylor","year":"2018","journal-title":"J. Neural Transm."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"966","DOI":"10.1017\/S1041610210000827","article-title":"Matrix Metalloproteinases in Peripheral Blood and Cerebrospinal Fluid in Patients with Alzheimer\u2019s Disease","volume":"22","author":"Horstmann","year":"2010","journal-title":"Int. Psychogeriatr."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"185","DOI":"10.3233\/JAD-130497","article-title":"BDNF, TNF\u03b1, HSP90, CFH, and IL-10 Serum Levels in Patients with Early or Late Onset Alzheimer\u2019s Disease or Mild Cognitive Impairment","volume":"37","author":"Dursun","year":"2013","journal-title":"J. Alzheimer\u2019s Dis."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.cbi.2018.02.002","article-title":"Ginsenoside F1 Suppresses Astrocytic Senescence-Associated Secretory Phenotype","volume":"283","author":"Hou","year":"2018","journal-title":"Chem. Biol. Interact."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"1016053","DOI":"10.3389\/fnagi.2022.1016053","article-title":"Necroptosis in CNS Diseases: Focus on Astrocytes","volume":"14","author":"Mitroshina","year":"2023","journal-title":"Front. Aging Neurosci"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"453901","DOI":"10.1155\/2015\/453901","article-title":"TrkB-Mediated Neuroprotective and Antihypoxic Properties of Brain-Derived Neurotrophic Factor","volume":"2015","author":"Vedunova","year":"2015","journal-title":"Oxid. Med. Cell Longev."},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Kustikova, V., Krivonosov, M., Pimashkin, A., Denisov, P., Zaikin, A., Ivanchenko, M., Meyerov, I., and Semyanov, A. (2018, January 5\u20137). CalciumCV: Computer Vision Software for Calcium Signaling in Astrocytes. Proceedings of the Analysis of Images, Social Networks and Texts: 7th International Conference, AIST 2018, Moscow, Russia.","DOI":"10.1007\/978-3-030-11027-7_17"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/15\/12286\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T20:23:32Z","timestamp":1760127812000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/15\/12286"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,7,31]]},"references-count":58,"journal-issue":{"issue":"15","published-online":{"date-parts":[[2023,8]]}},"alternative-id":["ijms241512286"],"URL":"https:\/\/doi.org\/10.3390\/ijms241512286","relation":{},"ISSN":["1422-0067"],"issn-type":[{"type":"electronic","value":"1422-0067"}],"subject":[],"published":{"date-parts":[[2023,7,31]]}}}