{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T22:35:11Z","timestamp":1789079711183,"version":"build-2803163510"},"reference-count":62,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2023,8,3]],"date-time":"2023-08-03T00:00:00Z","timestamp":1691020800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004281","name":"NATIONAL SCIENCE CENTRE OF POLAND","doi-asserted-by":"publisher","award":["2018\/31\/N\/NZ9\/03255"],"award-info":[{"award-number":["2018\/31\/N\/NZ9\/03255"]}],"id":[{"id":"10.13039\/501100004281","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The study aimed to provide the chemical, aroma and prohealth characteristics of the kaffir lime juice. A procedure using solid-phase microextraction with gas chromatography (SPME-GC-TOFMS) was optimized and validated for the determination of terpenes of kaffir lime. Main physicochemical parameters: pH, vitamin C, citric acid and \u00b0Brix were evaluated. Micro- and macro elements were determined using microwave plasma optic emission spectrometry (MP-OES). The binding of kaffir lime terpenes to human serum albumin (HSA) was investigated by fluorescence spectroscopy (3D-FL). \u03b2-Pinene and Limonene were selected as the most abundant terpenes with the concentration of 1225 \u00b1 35 and 545 \u00b1 16 \u00b5g\/g, respectively. The values of citric acid, vitamin C, \u00b0Brix and pH were 74.74 \u00b1 0.50 g\/kg, 22.31 \u00b1 0.53 mg\/100 mL, 10.35 \u00b1 0.70 and 2.406 \u00b1 0.086 for, respectively. Iron, with a concentration of 16.578 \u00b1 0.029 mg\/kg, was the most abundant microelement. Among the macroelements, potassium (8121 \u00b1 52 mg\/kg) was dominant. Kaffir lime binding to HSA was higher than \u03b2-Pinene, which may indicate the therapeutic effect of the juice. Kaffir lime juice is a source of terpenes with good aromatic and bioactive properties. Fluorescence measurements confirmed its therapeutic effect. Kaffir lime juice is also a good source of citric acid with potential industrial application. The high content of minerals compared to other citruses increases its prohealth value.<\/jats:p>","DOI":"10.3390\/ijms241512410","type":"journal-article","created":{"date-parts":[[2023,8,3]],"date-time":"2023-08-03T11:08:43Z","timestamp":1691060923000},"page":"12410","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Chemical, Aroma and Pro-Health Characteristics of Kaffir Lime Juice\u2014The Approach Using Optimized HS-SPME-GC-TOFMS, MP-OES, 3D-FL and Physiochemical Analysis"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1517-7696","authenticated-orcid":false,"given":"Martyna","family":"Lubinska-Szczyge\u0142","sequence":"first","affiliation":[{"name":"Department of Analytical Chemistry, Faculty of Chemistry, Gda\u0144sk University of Technology, 80-233 Gdansk, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2730-0068","authenticated-orcid":false,"given":"\u017baneta","family":"Polkowska","sequence":"additional","affiliation":[{"name":"Department of Analytical Chemistry, Faculty of Chemistry, Gda\u0144sk University of Technology, 80-233 Gdansk, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9837-4785","authenticated-orcid":false,"given":"Ma\u0142gorzata","family":"Rutkowska","sequence":"additional","affiliation":[{"name":"Department of Analytical Chemistry, Faculty of Chemistry, Gda\u0144sk University of Technology, 80-233 Gdansk, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0621-8305","authenticated-orcid":false,"given":"Shela","family":"Gorinstein","sequence":"additional","affiliation":[{"name":"Institute for Drug Research, School of Pharmacy, Hadassah Medical School, The Hebrew University, Jerusalem 91120, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,8,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Lubinska-Szczyge\u0142, M., Kuczy\u0144ska-\u0141a\u017cewska, A., Rutkowska, M., Polkowska, \u017b., Katrich, E., and Gorinstein, S. 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