{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T06:44:02Z","timestamp":1790837042911,"version":"4.1.0"},"reference-count":25,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2023,8,4]],"date-time":"2023-08-04T00:00:00Z","timestamp":1691107200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP21464465"],"award-info":[{"award-number":["JP21464465"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JPMXS0410300220"],"award-info":[{"award-number":["JPMXS0410300220"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"name":"MEXT Project for promoting public utilization of advanced research infrastructure (Imaging Platform) OF FUNDER","award":["JP21464465"],"award-info":[{"award-number":["JP21464465"]}]},{"name":"MEXT Project for promoting public utilization of advanced research infrastructure (Imaging Platform) OF FUNDER","award":["JPMXS0410300220"],"award-info":[{"award-number":["JPMXS0410300220"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>To understand the ultra-early reaction of normal organ lipids during irradiation, we investigated the response of lipids, including polyunsaturated fatty acid (PUFA) chains, which are particularly susceptible to damage by ROS, in mice\u2019s kidneys, lungs, brains, and livers within 5 min of single high-dose irradiation. In this study, we set up three groups of C56BL\/6 male mice and conducted whole-body irradiation with 0 Gy, 10 Gy, and 20 Gy single doses. Kidney, lung, brain, and liver tissues were collected within 5 min of irradiation. PUFA-targeted and whole lipidomic analyses were conducted using liquid chromatography\u2013tandem mass spectrometry (LC-MS\/MS). The results showed that PUFA chains of kidney phosphatidylcholine (PC), phosphatidylethanolamine (PE), and triacylglycerol (TG) significantly increased within 5 min of 10 Gy and 20 Gy irradiation. The main components of increased PUFA chains in PC and PE were C18:2, C20:4, and C22:6, and in TG the main component was C18:2. The kidney lipidomes also showed significant changes from the perspective of lipid species, mainly dominated by an increase in PC, PE, TG, and signal lipids, while lipidomes of the lung, brain, and liver were slightly changed. Our results revealed that acute PUFA chains increase and other lipidomic changes in the kidney upon whole-body irradiation within 5 min of irradiation. The significantly increased lipids also showed a consistent preference for possessing PUFA chains. The lipidomic changes varied from organ to organ, which indicates that the response upon irradiation within a short time is tissue-specific.<\/jats:p>","DOI":"10.3390\/ijms241512439","type":"journal-article","created":{"date-parts":[[2023,8,4]],"date-time":"2023-08-04T09:27:48Z","timestamp":1691141268000},"page":"12439","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Lipid Polyunsaturated Fatty Acid Chains in Mouse Kidneys Were Increased within 5 min of a Single High Dose Whole Body Irradiation"],"prefix":"10.3390","volume":"24","author":[{"given":"Wenxin","family":"Li","sequence":"first","affiliation":[{"name":"Department of Radiation Oncology, Hamamatsu University School of Medicine, Handayama 1-20-1, Higashi-ku, Hamamatsu 431-3192, Shizuoka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8889-2044","authenticated-orcid":false,"given":"Chi","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Cellular and Molecular Anatomy, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu 431-3192, Shizuoka, Japan"},{"name":"Department of Systems Molecular Anatomy, Institute for Medical Photonics Research, Preeminent Medical Photonics Education & Research Center Hamamatsu, Hamamatsu 431-3192, Shizuoka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5842-6176","authenticated-orcid":false,"given":"Shuhei","family":"Aramaki","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology, Hamamatsu University School of Medicine, Handayama 1-20-1, Higashi-ku, Hamamatsu 431-3192, Shizuoka, Japan"},{"name":"Department of Cellular and Molecular Anatomy, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu 431-3192, Shizuoka, Japan"},{"name":"International Mass Imaging Center, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-Ku, Hamamatsu 431-3192, Shizuoka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5340-8841","authenticated-orcid":false,"given":"Lili","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Cellular and Molecular Anatomy, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu 431-3192, Shizuoka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shogo","family":"Tsuge","sequence":"additional","affiliation":[{"name":"Department of Cellular and Molecular Anatomy, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu 431-3192, Shizuoka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2271-9975","authenticated-orcid":false,"given":"Takumi","family":"Sakamoto","sequence":"additional","affiliation":[{"name":"Department of Cellular and Molecular Anatomy, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu 431-3192, Shizuoka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9274-3386","authenticated-orcid":false,"given":"Md. Al","family":"Mamun","sequence":"additional","affiliation":[{"name":"Department of Cellular and Molecular Anatomy, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu 431-3192, Shizuoka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7497-3540","authenticated-orcid":false,"given":"Ariful","family":"Islam","sequence":"additional","affiliation":[{"name":"Department of Cellular and Molecular Anatomy, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu 431-3192, Shizuoka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takamitsu","family":"Hayakawa","sequence":"additional","affiliation":[{"name":"First Department of Surgery, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu 431-3192, Shizuoka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yusuke","family":"Takanashi","sequence":"additional","affiliation":[{"name":"First Department of Surgery, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu 431-3192, Shizuoka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7790-2537","authenticated-orcid":false,"given":"Maxime","family":"Dubail","sequence":"additional","affiliation":[{"name":"Institut Curie, Centre de Recherche, INSERM U612, 91405 Orsay, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenta","family":"Konishi","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology, Hamamatsu University School of Medicine, Handayama 1-20-1, Higashi-ku, Hamamatsu 431-3192, Shizuoka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomohito","family":"Sato","sequence":"additional","affiliation":[{"name":"Department of Cellular and Molecular Anatomy, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu 431-3192, Shizuoka, Japan"},{"name":"International Mass Imaging Center, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-Ku, Hamamatsu 431-3192, Shizuoka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2205-5665","authenticated-orcid":false,"given":"Tomoaki","family":"Kahyo","sequence":"additional","affiliation":[{"name":"Department of Cellular and Molecular Anatomy, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu 431-3192, Shizuoka, Japan"},{"name":"International Mass Imaging Center, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-Ku, Hamamatsu 431-3192, Shizuoka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles","family":"Fouillade","sequence":"additional","affiliation":[{"name":"Institut Curie, Centre de Recherche, INSERM U612, 91405 Orsay, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katsumasa","family":"Nakamura","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology, Hamamatsu University School of Medicine, Handayama 1-20-1, Higashi-ku, Hamamatsu 431-3192, Shizuoka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1302-6467","authenticated-orcid":false,"given":"Mitsutoshi","family":"Setou","sequence":"additional","affiliation":[{"name":"Department of Cellular and Molecular Anatomy, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu 431-3192, Shizuoka, Japan"},{"name":"Department of Systems Molecular Anatomy, Institute for Medical Photonics Research, Preeminent Medical Photonics Education & Research Center Hamamatsu, Hamamatsu 431-3192, Shizuoka, Japan"},{"name":"International Mass Imaging Center, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-Ku, Hamamatsu 431-3192, Shizuoka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,8,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1200\/JGO.2015.001545","article-title":"Global Access to Radiotherapy Services: Have We Made Progress During the Past Decade?","volume":"2","author":"Yap","year":"2016","journal-title":"J. Glob. Oncol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1165","DOI":"10.3389\/fonc.2020.01165","article-title":"Advances in Radiobiology of Stereotactic Ablative Radiotherapy","volume":"10","author":"Qiu","year":"2020","journal-title":"Front. Oncol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.addr.2016.11.005","article-title":"Stereotactic ablative body radiosurgery (SABR) or Stereotactic body radiation therapy (SBRT)","volume":"109","author":"Folkert","year":"2017","journal-title":"Adv. Drug Deliv. Rev."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1016\/j.clon.2015.02.001","article-title":"The radiobiology of hypofractionation","volume":"27","author":"Nahum","year":"2015","journal-title":"Clin. Oncol. (R. Coll. Radiol. (Great Br.))"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"888","DOI":"10.1016\/j.cell.2010.11.033","article-title":"Lipidomics: New tools and applications","volume":"143","author":"Wenk","year":"2010","journal-title":"Cell"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"19","DOI":"10.3390\/metabo2010019","article-title":"Mass Spectrometry Based Lipidomics: An Overview of Technological 391 Platforms","volume":"2","author":"Fauland","year":"2012","journal-title":"Metabolites"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/j.canlet.2017.05.002","article-title":"Targeting lipid metabolism of cancer cells: A promising therapeutic strategy for cancer","volume":"401","author":"Liu","year":"2017","journal-title":"Cancer Lett."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"5944","DOI":"10.1021\/cr200084z","article-title":"Free radical lipid peroxidation: Mechanisms and analysis","volume":"111","author":"Yin","year":"2011","journal-title":"Chem. Rev."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1016\/j.freeradbiomed.2009.05.032","article-title":"Lipid peroxidation: Physiological levels and dual biological effects","volume":"47","author":"Niki","year":"2009","journal-title":"Free Radic. Biol. Med."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1038\/s41586-018-0198-8","article-title":"Oxidized phospholipids are proinflammatory and proatherogenic in hypercholesterolaemic mice","volume":"558","author":"Que","year":"2018","journal-title":"Nature"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1038\/s41419-021-03613-y","article-title":"Lipid peroxidation and the subsequent cell death transmitting from ferroptotic cells to neighboring cells","volume":"12","author":"Nishizawa","year":"2021","journal-title":"Cell Death Dis."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"922","DOI":"10.1038\/s41419-020-03118-0","article-title":"Excessive phospholipid peroxidation distinguishes ferroptosis from other cell death modes including pyroptosis","volume":"11","author":"Wiernicki","year":"2020","journal-title":"Cell Death Dis."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1667\/0033-7587(2003)159[0471:TCMAAB]2.0.CO;2","article-title":"The cell membrane as a biosensor of oxidative stress induced by radiation exposure: A multiparameter investigation","volume":"159","author":"Benderitter","year":"2003","journal-title":"Radiat. Res."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"20984","DOI":"10.1038\/srep20984","article-title":"Integration of lipidomics and transcriptomics unravels aberrant lipid metabolism and defines cholesteryl oleate as potential biomarker of prostate cancer","volume":"6","author":"Li","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3389\/fcell.2021.675617","article-title":"Fatty Acids Metabolism: The Bridge Between Ferroptosis and Ionizing Radiation","volume":"9","author":"Yuan","year":"2021","journal-title":"Front. Cell Dev. Biol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"6807","DOI":"10.1038\/srep06807","article-title":"Direct Visualization of De novo Lipogenesis in Single Living Cells","volume":"4","author":"Li","year":"2014","journal-title":"Sci. Rep."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1042\/BST20210579","article-title":"New appreciation for an old pathway: The Lands Cycle moves into new arenas in health and disease","volume":"50","year":"2022","journal-title":"Biochem. Soc. Trans."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1111\/j.2042-3306.1984.tb01893.x","article-title":"The acute inflammatory process, arachidonic acid metabolism and the mode of action of anti-inflammatory drugs","volume":"16","author":"Higgins","year":"1984","journal-title":"Equine Vet. J."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"106881","DOI":"10.1016\/j.clineuro.2021.106881","article-title":"The stability of the metabolic turnover of arachidonic acid in human unruptured intracranial aneurysmal walls is sustained","volume":"208","author":"Takeda","year":"2021","journal-title":"Clin. Neurol. Neurosurg."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"168","DOI":"10.4103\/0973-1482.19597","article-title":"Analytical approach to estimate normal tissue complication probability using best fit of normal tissue tolerance doses into the NTCP equation of the linear quadratic model","volume":"1","author":"Kehwar","year":"2005","journal-title":"J. Cancer Res. Ther."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1016\/j.ijrobp.2013.10.015","article-title":"Alpha\/Beta Ratio for Normal Lung Tissue as Estimated From Lung Cancer Patients Treated With Stereotactic Body and Conventionally Fractionated Radiation Therapy","volume":"88","author":"Scheenstra","year":"2014","journal-title":"Int. J. Radiat. Oncol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"911","DOI":"10.1139\/y59-099","article-title":"A rapid method of total lipid extraction and purification","volume":"37","author":"Bligh","year":"1959","journal-title":"Can. J. Biochem. Physiol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"4229","DOI":"10.1016\/j.chroma.2010.04.034","article-title":"Precise and global identification of phospholipid molecular species by an Orbitrap mass spectrometer and automated search engine Lipid Search","volume":"1217","author":"Taguchi","year":"2010","journal-title":"J. Chromatogr. A"},{"key":"ref_24","unstructured":"Peake, D., Kiyonami, R., Yokoi, Y., Fukamachi, Y., and Huang, Y. (June, January 31). Processing of a Complex Lipid Dataset for the NIST Inter-laboratory Comparison Exercise for Lipidomics Measurements in Human Serum and Plasma. Proceedings of the LIPID MAPS Annual Meeting, Saint Louis, MO, USA."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1016\/S0076-6879(07)32008-9","article-title":"Basic Analytical Systems for Lipidomics by Mass Spectrometry in Japan","volume":"432","author":"Taguchi","year":"2007","journal-title":"Methods Enzymol."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/15\/12439\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T20:26:00Z","timestamp":1760127960000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/15\/12439"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,8,4]]},"references-count":25,"journal-issue":{"issue":"15","published-online":{"date-parts":[[2023,8]]}},"alternative-id":["ijms241512439"],"URL":"https:\/\/doi.org\/10.3390\/ijms241512439","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,8,4]]}}}