{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,26]],"date-time":"2026-01-26T01:50:35Z","timestamp":1769392235961,"version":"3.49.0"},"reference-count":83,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2023,8,5]],"date-time":"2023-08-05T00:00:00Z","timestamp":1691193600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Brazilian National Institute of Science and Technology for Coffee"},{"name":"Minas Gerais State Foundation of Support to Research"},{"name":"Brazilian National Council for Scientific and Technological Development"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The use of transcriptomic data to make inferences about plant metabolomes is a useful tool to help the discovery of important compounds in the available biodiversity. To unveil previously undiscovered metabolites of Coffea, of phytotherapeutic and economic value, we employed 24 RNAseq libraries. These libraries were sequenced from leaves exposed to a diverse range of environmental conditions. Subsequently, the data were meticulously processed to create models of putative metabolic networks, which shed light on the production of potential natural compounds of significant interest. Then, we selected one of the predicted compounds, the L-3,4-dihydroxyphenylalanine (L-DOPA), to be analyzed by LC\u2013MS\/MS using three biological replicates of flowers, leaves, and fruits from Coffea arabica and Coffea canephora. We were able to identify metabolic pathways responsible for producing several compounds of economic importance. One of the identified pathways involved in isoquinoline alkaloid biosynthesis was found to be active and producing L-DOPA, which is a common product of POLYPHENOL OXIDASES (PPOs, EC 1.14.18.1 and EC 1.10.3.1). We show that coffee plants are a natural source of L-DOPA, a widely used medicine for treatment of the human neurodegenerative condition called Parkinson\u2019s disease. In addition, dozens of other compounds with medicinal significance were predicted as potential natural coffee products. By further refining analytical chemistry techniques, it will be possible to enhance the characterization of coffee metabolites, enabling a deeper understanding of their properties and potential applications in medicine.<\/jats:p>","DOI":"10.3390\/ijms241512466","type":"journal-article","created":{"date-parts":[[2023,8,5]],"date-time":"2023-08-05T10:21:39Z","timestamp":1691230899000},"page":"12466","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Metabolic Pathway Reconstruction Indicates the Presence of Important Medicinal Compounds in Coffea Such as L-DOPA"],"prefix":"10.3390","volume":"24","author":[{"given":"Thales Henrique","family":"Cherubino Ribeiro","sequence":"first","affiliation":[{"name":"Laboratory of Plant Molecular Physiology, Plant Physiology Sector, Department of Biology, Federal University of Lavras (UFLA), Lavras 37200-000, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3971-0021","authenticated-orcid":false,"given":"Raphael Ricon","family":"de Oliveira","sequence":"additional","affiliation":[{"name":"Laboratory of Plant Molecular Physiology, Plant Physiology Sector, Department of Biology, Federal University of Lavras (UFLA), Lavras 37200-000, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ta\u00eds Teixeira","family":"das Neves","sequence":"additional","affiliation":[{"name":"Plant Physiology Sector, Department of Biology, Federal University of Lavras (UFLA), Lavras 37200-000, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wilder Douglas","family":"Santiago","sequence":"additional","affiliation":[{"name":"National Institute of Coffee Science and Technology (INCT-CAF\u00c9), Federal University of Lavras (UFLA), Lavras 37200-000, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bethania Leite","family":"Mansur","sequence":"additional","affiliation":[{"name":"Multiuser Instrumental Analysis Laboratory (LabMAI), Federal University of Lavras (UFLA), Lavras 37200-000, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adelir Aparecida","family":"Saczk","sequence":"additional","affiliation":[{"name":"Analytical and Electroanalytical Laboratory (LAE), Federal University of Lavras (UFLA), Lavras 37200-000, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mario Lucio","family":"Vilela de Resende","sequence":"additional","affiliation":[{"name":"Plant Pathology Department, Federal University of Lavras (UFLA), Lavras 37200-000, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1794-9429","authenticated-orcid":false,"given":"Antonio","family":"Chalfun-Junior","sequence":"additional","affiliation":[{"name":"Laboratory of Plant Molecular Physiology, Plant Physiology Sector, Department of Biology, Federal University of Lavras (UFLA), Lavras 37200-000, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,8,5]]},"reference":[{"key":"ref_1","unstructured":"(2022, August 22). 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