{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T22:55:23Z","timestamp":1784933723175,"version":"3.55.0"},"reference-count":50,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2023,8,7]],"date-time":"2023-08-07T00:00:00Z","timestamp":1691366400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"MINISTRY OF SCIENCE AND INNOVATION (MCIN\/AEI\/10.13039\/501100011033)","award":["PID2020-116495RB-I00"],"award-info":[{"award-number":["PID2020-116495RB-I00"]}]},{"name":"MINISTRY OF SCIENCE AND INNOVATION (MCIN\/AEI\/10.13039\/501100011033)","award":["IT1578-22, GIC21\/18"],"award-info":[{"award-number":["IT1578-22, GIC21\/18"]}]},{"name":"MINISTRY OF SCIENCE AND INNOVATION (MCIN\/AEI\/10.13039\/501100011033)","award":["2072"],"award-info":[{"award-number":["2072"]}]},{"DOI":"10.13039\/501100003086","name":"DEPARTMENT OF EDUCATION OF THE BASQUE GOVERNMENT (Research Groups of the Basque University System 2021)","doi-asserted-by":"publisher","award":["PID2020-116495RB-I00"],"award-info":[{"award-number":["PID2020-116495RB-I00"]}],"id":[{"id":"10.13039\/501100003086","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003086","name":"DEPARTMENT OF EDUCATION OF THE BASQUE GOVERNMENT (Research Groups of the Basque University System 2021)","doi-asserted-by":"publisher","award":["IT1578-22, GIC21\/18"],"award-info":[{"award-number":["IT1578-22, GIC21\/18"]}],"id":[{"id":"10.13039\/501100003086","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003086","name":"DEPARTMENT OF EDUCATION OF THE BASQUE GOVERNMENT (Research Groups of the Basque University System 2021)","doi-asserted-by":"publisher","award":["2072"],"award-info":[{"award-number":["2072"]}],"id":[{"id":"10.13039\/501100003086","id-type":"DOI","asserted-by":"publisher"}]},{"name":"ARAB ACADEMY FOR SCIENCE, TECHNOLOGY AND MARITIME TRANSPORT","award":["PID2020-116495RB-I00"],"award-info":[{"award-number":["PID2020-116495RB-I00"]}]},{"name":"ARAB ACADEMY FOR SCIENCE, TECHNOLOGY AND MARITIME TRANSPORT","award":["IT1578-22, GIC21\/18"],"award-info":[{"award-number":["IT1578-22, GIC21\/18"]}]},{"name":"ARAB ACADEMY FOR SCIENCE, TECHNOLOGY AND MARITIME TRANSPORT","award":["2072"],"award-info":[{"award-number":["2072"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The increasing rates of antimicrobial resistance among carbapenem-resistant Acinetobacter baumannii in the Middle East and North Africa are one of the major concerns for healthcare settings. We characterised the first A. baumannii isolate harbouring five \u03b2-lactamases identified in Egypt. The isolate Ale25 was obtained from an ICU patient of a hospital from Alexandria. The isolate was phenotypically and genotypically screened for carbapenemase genes. The isolate was resistant to carbapenems, aminoglycosides, fluoroquinolones and cefiderocol. Whole-Genome Sequencing identified five \u03b2-lactamase genes, blaNDM-1, blaOXA-23, blaOXA-64, blaPER-7 and blaADC-57, together with other antibiotic resistance genes, conferring resistance to sulfonamides, macrolides, tetracyclines, rifamycin and chloramphenicol. Virulome analysis showed the presence of genes involved in adhesion and biofilm production, type II and VI secretion systems, exotoxins, etc. Multi-Locus Sequence Typing analysis identified the isolate as Sequence Types 113Pas and 2246Oxf, belonging to International Clone 7. Sequencing experiments revealed the presence of four plasmids of 2.7, 22.3, 70.4 and 240.8 Kb. All the \u03b2-lactamase genes were located in the chromosome, except the blaPER-7, gene which was found within the plasmid of 240.8 Kb. This study highlights the threat of the emergence and dissemination of these types of isolates.<\/jats:p>","DOI":"10.3390\/ijms241512515","type":"journal-article","created":{"date-parts":[[2023,8,7]],"date-time":"2023-08-07T06:37:31Z","timestamp":1691390251000},"page":"12515","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Co-Existence of blaNDM-1, blaOXA-23, blaOXA-64, blaPER-7 and blaADC-57 in a Clinical Isolate of Acinetobacter baumannii from Alexandria, Egypt"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8435-839X","authenticated-orcid":false,"given":"Sandra","family":"S\u00e1nchez-Urtaza","sequence":"first","affiliation":[{"name":"Department of Immunology, Microbiology and Parasitology, Faculty of Medicine and Nursing, University of the Basque Country, 48940 Leioa, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alain","family":"Ocampo-Sosa","sequence":"additional","affiliation":[{"name":"Microbiology Service, Health Research Institute (IDIVAL), University Hospital Marqu\u00e9s de Valdecilla, 39008 Santander, Spain"},{"name":"CIBER de Enfermedades Infecciosas (CIBERINFEC), Instituto de Salud Carlos III, 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ainhoa","family":"Molins-Bengoetxea","sequence":"additional","affiliation":[{"name":"Department of Immunology, Microbiology and Parasitology, Faculty of Medicine and Nursing, University of the Basque Country, 48940 Leioa, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8229-6049","authenticated-orcid":false,"given":"Jorge","family":"Rodr\u00edguez-Grande","sequence":"additional","affiliation":[{"name":"Microbiology Service, Health Research Institute (IDIVAL), University Hospital Marqu\u00e9s de Valdecilla, 39008 Santander, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4944-5962","authenticated-orcid":false,"given":"Mohammed A.","family":"El-Kholy","sequence":"additional","affiliation":[{"name":"Division of Clinical and Biological Sciences, Department of Microbiology and Biotechnology, College of Pharmacy, Arab Academy for Science, Technology & Maritime Transport (AASTMT), Alexandria P.O. Box 1029, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6914-9987","authenticated-orcid":false,"given":"Marta","family":"Hernandez","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Biology and Microbiology, One Health, Agrarian Technological Institute of Castile and Leon (ITACyL), 47009 Valladolid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5423-3537","authenticated-orcid":false,"given":"David","family":"Abad","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Biology and Microbiology, One Health, Agrarian Technological Institute of Castile and Leon (ITACyL), 47009 Valladolid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sherine M.","family":"Shawky","sequence":"additional","affiliation":[{"name":"Medical Research Institute, Alexandria University, Alexandria 5422031, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6978-389X","authenticated-orcid":false,"given":"Itziar","family":"Alkorta","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Faculty of Science and Technology, University of the Basque Country, 48940 Leioa, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2885-7789","authenticated-orcid":false,"given":"Lucia","family":"Gallego","sequence":"additional","affiliation":[{"name":"Department of Immunology, Microbiology and Parasitology, Faculty of Medicine and Nursing, University of the Basque Country, 48940 Leioa, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,8,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"589253","DOI":"10.3389\/fmicb.2020.589253","article-title":"First Report of New Delhi Metallo-\u0392-Lactamase-6 (NDM-6) in a Clinical Acinetobacter baumannii Isolate from Northern Spain","volume":"11","author":"Xanthopoulou","year":"2020","journal-title":"Front. 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