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The contribution of the TRPA1-dependent activity to basal tearing and thermally-evoked blink was also explored. The corneal cold thermoreceptors\u2019 activity was recorded extracellularly in young (5-month-old) and aged (18-month-old) C57BL\/6WT (WT) and TRPA1\u2212\/\u2212 knockout (TRPA1-KO) mice at basal temperature (34 \u00b0C) and during cooling (15 \u00b0C) and heating (45 \u00b0C) ramps. The blink response to cold and heat stimulation of the ocular surface and the basal tearing rate were also measured in young animals using orbicularis oculi muscle electromyography (OOemg) and phenol red threads, respectively. The background activity at 34 \u00b0C and the cooling- and heating-evoked responses of the cold thermoreceptors were similar in WT and TRPA1-KO animals, no matter the age. Similar to the aged WT mice, in the young and aged TRPA1-KO mice, most of the cold thermoreceptors presented low frequency background activity, a low cooling threshold, and a sluggish response to heating. The amplitude and duration of the OOemg signals correlated with the magnitude of the induced thermal change in the WT but not in the TRPA1-KO mice. The basal tearing was similar in the TRPA1-KO and WT mice. The electrophysiological data suggest that the TRPA1-dependent nerve activity, which declines with age, contributes to detecting the warming of the ocular surface and also to integrating the thermally-evoked reflex blink.<\/jats:p>","DOI":"10.3390\/ijms241612620","type":"journal-article","created":{"date-parts":[[2023,8,9]],"date-time":"2023-08-09T10:21:50Z","timestamp":1691576510000},"page":"12620","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["The Contribution of TRPA1 to Corneal Thermosensitivity and Blink Regulation in Young and Aged Mice"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0768-3735","authenticated-orcid":false,"given":"Laura","family":"Frutos-Rinc\u00f3n","sequence":"first","affiliation":[{"name":"Instituto de Neurociencias, Universidad Miguel Hern\u00e1ndez-Consejo Superior de Investigaciones Cient\u00edficas, 03550 San Juan de Alicante, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolina","family":"Luna","sequence":"additional","affiliation":[{"name":"Instituto de Neurociencias, Universidad Miguel Hern\u00e1ndez-Consejo Superior de Investigaciones Cient\u00edficas, 03550 San Juan de Alicante, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4739-5033","authenticated-orcid":false,"given":"Fernando","family":"Aleixandre-Carrera","sequence":"additional","affiliation":[{"name":"Instituto de Neurociencias, Universidad Miguel Hern\u00e1ndez-Consejo Superior de Investigaciones Cient\u00edficas, 03550 San Juan de Alicante, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7299-0750","authenticated-orcid":false,"given":"Enrique","family":"Velasco","sequence":"additional","affiliation":[{"name":"Instituto de Neurociencias, Universidad Miguel Hern\u00e1ndez-Consejo Superior de Investigaciones Cient\u00edficas, 03550 San Juan de Alicante, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ariadna","family":"Diaz-Tahoces","sequence":"additional","affiliation":[{"name":"Instituto de Neurociencias, Universidad Miguel Hern\u00e1ndez-Consejo Superior de Investigaciones Cient\u00edficas, 03550 San Juan de Alicante, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7686-6228","authenticated-orcid":false,"given":"V\u00edctor","family":"Meseguer","sequence":"additional","affiliation":[{"name":"Instituto de Neurociencias, Universidad Miguel Hern\u00e1ndez-Consejo Superior de Investigaciones Cient\u00edficas, 03550 San Juan de Alicante, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3559-3649","authenticated-orcid":false,"given":"Juana","family":"Gallar","sequence":"additional","affiliation":[{"name":"Instituto de Neurociencias, Universidad Miguel Hern\u00e1ndez-Consejo Superior de Investigaciones Cient\u00edficas, 03550 San Juan de Alicante, Spain"},{"name":"Instituto de Investigaci\u00f3n Biom\u00e9dica y Sanitaria de Alicante, 03010 Alicante, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1759-068X","authenticated-orcid":false,"given":"M. Carmen","family":"Acosta","sequence":"additional","affiliation":[{"name":"Instituto de Neurociencias, Universidad Miguel Hern\u00e1ndez-Consejo Superior de Investigaciones Cient\u00edficas, 03550 San Juan de Alicante, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,8,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1007\/s00424-012-1158-z","article-title":"The transient receptor potential channel TRPA1: From gene to pathophysiology","volume":"464","author":"Nilius","year":"2012","journal-title":"Pflugers Arch. Eur. J. 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