{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T13:07:18Z","timestamp":1780405638840,"version":"3.54.1"},"reference-count":28,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2023,8,11]],"date-time":"2023-08-11T00:00:00Z","timestamp":1691712000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005297","name":"Medical University of Bialystok","doi-asserted-by":"publisher","award":["SUB\/1\/DN\/21\/002\/1157"],"award-info":[{"award-number":["SUB\/1\/DN\/21\/002\/1157"]}],"id":[{"id":"10.13039\/501100005297","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005297","name":"Medical University of Bialystok","doi-asserted-by":"publisher","award":["SUB\/1\/NN\/21\/002\/4406"],"award-info":[{"award-number":["SUB\/1\/NN\/21\/002\/4406"]}],"id":[{"id":"10.13039\/501100005297","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The aim of this study was to compare the aqueous humor (AH) and serum concentrations of metabolites in diabetic (n = 36) and nondiabetic (n = 36) senior adults undergoing cataract surgery. Blood samples were collected before surgery and AH during surgery. Liquid chromatography coupled with tandem mass spectrometry (LC-MS\/MS)-based targeted metabolomic and lipidomic analyses of samples were performed using the AbsoluteIDQ\u00ae p180 kit. Out of 188 metabolites targeted by the kit, 41 and 133 were detected in &gt;80% of AH and serum samples, respectively. Statistical analysis performed to indicate metabolites differentiating diabetic and nondiabetic patients showed 8 and 20 significant metabolites in AH and serum, respectively. Pathway analysis performed for significant metabolites revealed that galactose metabolism is mostly affected in the AH, while arginine biosynthesis is mostly affected in the serum. Among metabolites that differentiate diabetic and nondiabetic patients, arginine was the only metabolite common to both serum and AH samples, as well as the only one with a decreased concentration in both body fluids of diabetic patients. Concentrations of the rest were elevated in AH and lowered in serum. This may suggest different mechanisms of diabetes-related dysregulation of the local metabolism in the eye in comparison to systemic changes observed in the blood.<\/jats:p>","DOI":"10.3390\/ijms241612671","type":"journal-article","created":{"date-parts":[[2023,8,11]],"date-time":"2023-08-11T10:20:16Z","timestamp":1691749216000},"page":"12671","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Simultaneous Comparison of Aqueous Humor and Serum Metabolic Profiles of Diabetic and Nondiabetic Patients Undergoing Cataract Surgery\u2014A Targeted and Quantitative Metabolomics Study"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8235-1263","authenticated-orcid":false,"given":"Emil Tomasz","family":"Grochowski","sequence":"first","affiliation":[{"name":"Department of Ophthalmology, Medical University of Bialystok, M. Sklodowskiej Curie 24a, 15-276 Bialystok, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6639-2823","authenticated-orcid":false,"given":"Karolina","family":"Pietrowska","sequence":"additional","affiliation":[{"name":"Metabolomics Laboratory, Clinical Research Center, Medical University of Bialystok, M. Sklodowskiej Curie 24a, 15-276 Bialystok, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2147-2552","authenticated-orcid":false,"given":"Adrian","family":"Godlewski","sequence":"additional","affiliation":[{"name":"Metabolomics Laboratory, Clinical Research Center, Medical University of Bialystok, M. Sklodowskiej Curie 24a, 15-276 Bialystok, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wioleta","family":"Gosk","sequence":"additional","affiliation":[{"name":"Metabolomics Laboratory, Clinical Research Center, Medical University of Bialystok, M. 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Sklodowskiej Curie 24a, 15-276 Bialystok, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam","family":"Kretowski","sequence":"additional","affiliation":[{"name":"Metabolomics Laboratory, Clinical Research Center, Medical University of Bialystok, M. Sklodowskiej Curie 24a, 15-276 Bialystok, Poland"},{"name":"Department of Endocrinology, Diabetology and Internal Medicine, Medical University of Bialystok, M. Sklodowskiej Curie 24a, 15-276 Bialystok, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3729-0518","authenticated-orcid":false,"given":"Michal","family":"Ciborowski","sequence":"additional","affiliation":[{"name":"Metabolomics Laboratory, Clinical Research Center, Medical University of Bialystok, M. Sklodowskiej Curie 24a, 15-276 Bialystok, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3548-9139","authenticated-orcid":false,"given":"Diana Anna","family":"Dmuchowska","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Medical University of Bialystok, M. Sklodowskiej Curie 24a, 15-276 Bialystok, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,8,11]]},"reference":[{"key":"ref_1","unstructured":"CDC (2022, August 28). 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