{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T05:18:34Z","timestamp":1785561514298,"version":"3.56.0"},"reference-count":61,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2023,8,14]],"date-time":"2023-08-14T00:00:00Z","timestamp":1691971200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"special projects in key fields of colleges and universities in Guangdong Province","award":["2022ZDZX2026"],"award-info":[{"award-number":["2022ZDZX2026"]}]},{"name":"special projects in key fields of colleges and universities in Guangdong Province","award":["2022A01033"],"award-info":[{"award-number":["2022A01033"]}]},{"name":"special projects in key fields of colleges and universities in Guangdong Province","award":["2019YFD0901805"],"award-info":[{"award-number":["2019YFD0901805"]}]},{"name":"special funds for the scientific and technological development of Zhanjiang","award":["2022ZDZX2026"],"award-info":[{"award-number":["2022ZDZX2026"]}]},{"name":"special funds for the scientific and technological development of Zhanjiang","award":["2022A01033"],"award-info":[{"award-number":["2022A01033"]}]},{"name":"special funds for the scientific and technological development of Zhanjiang","award":["2019YFD0901805"],"award-info":[{"award-number":["2019YFD0901805"]}]},{"name":"National Key R&amp;D Program of China","award":["2022ZDZX2026"],"award-info":[{"award-number":["2022ZDZX2026"]}]},{"name":"National Key R&amp;D Program of China","award":["2022A01033"],"award-info":[{"award-number":["2022A01033"]}]},{"name":"National Key R&amp;D Program of China","award":["2019YFD0901805"],"award-info":[{"award-number":["2019YFD0901805"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Tilapia skin is a great source of collagen. Here, we aimed to isolate and identify the peptides responsible for combating dry eye disease (DED) in tilapia skin peptides (TSP). In vitro cell DED model was used to screen anti-DED peptides from TSP via Sephadex G-25 chromatography, LC\/MS\/MS, and in silico methods. The anti-DED activity of the screened peptide was further verified in the mice DED model. TSP was divided into five fractions (TSP-I, TSP-II, TSP-III, TSP-IV, and TSP-V), and TSP-II exerted an effective effect for anti-DED. A total of 131 peptides were identified using LC\/MS\/MS in TSP-II, and NGGPSGPR (NGG) was screened as a potential anti-DED fragment in TSP-II via in silico methods. In vitro, NGG restored cell viability and inhibited the expression level of Cyclooxygenase-2 (COX-2) protein in Human corneal epithelial cells (HCECs) induced by NaCl. In vivo, NGG increased tear production, decreased tear ferning score, prevented corneal epithelial thinning, alleviated conjunctival goblet cell loss, and inhibited the apoptosis of corneal epithelial cells in DED mice. Overall, NGG, as an anti-DED peptide, was successfully identified from TSP, and it may be devoted to functional food ingredients or medicine for DED.<\/jats:p>","DOI":"10.3390\/ijms241612772","type":"journal-article","created":{"date-parts":[[2023,8,14]],"date-time":"2023-08-14T10:13:57Z","timestamp":1692008037000},"page":"12772","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Isolation and Identification of a Novel Anti-Dry Eye Peptide from Tilapia Skin Peptides Based on In Silico, In Vitro, and In Vivo Approaches"],"prefix":"10.3390","volume":"24","author":[{"given":"Jian","family":"Zeng","sequence":"first","affiliation":[{"name":"College of Food Science and Technology, Modern Biochemistry Experimental Center, Guangdong Ocean University, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Zhanjiang 524088, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cuixian","family":"Lin","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology, Modern Biochemistry Experimental Center, Guangdong Ocean University, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Zhanjiang 524088, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shilin","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology, Modern Biochemistry Experimental Center, Guangdong Ocean University, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Zhanjiang 524088, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haowen","family":"Yin","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology, Modern Biochemistry Experimental Center, Guangdong Ocean University, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Zhanjiang 524088, China"},{"name":"College of Food Science and Engineering, Ocean University of China, Yu-Shan Road, Qingdao 266003, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaishu","family":"Deng","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology, Modern Biochemistry Experimental Center, Guangdong Ocean University, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Zhanjiang 524088, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiyou","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology, Modern Biochemistry Experimental Center, Guangdong Ocean University, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Zhanjiang 524088, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongping","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology, Modern Biochemistry Experimental Center, Guangdong Ocean University, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Zhanjiang 524088, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"You","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology, Modern Biochemistry Experimental Center, Guangdong Ocean University, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Zhanjiang 524088, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuanyin","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Biology, Guangdong Medical University, Zhanjiang 524023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0378-2880","authenticated-orcid":false,"given":"Yun-Tao","family":"Zhao","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology, Modern Biochemistry Experimental Center, Guangdong Ocean University, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Zhanjiang 524088, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,8,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"114498","DOI":"10.1016\/j.bios.2022.114498","article-title":"Rapid and quantitative detection of tear MMP-9 for dry eye patients using a novel silicon nanowire-based biosensor","volume":"214","author":"Lu","year":"2022","journal-title":"Biosens. 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