{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T02:25:39Z","timestamp":1760149539881,"version":"build-2065373602"},"reference-count":48,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2023,8,18]],"date-time":"2023-08-18T00:00:00Z","timestamp":1692316800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior-Brasil (CAPES)","doi-asserted-by":"publisher","award":["001"],"award-info":[{"award-number":["001"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Although the association of polyols\/polyphosphates\/fluoride has been demonstrated to promote remarkable effects on dental enamel, little is known on their combined effects on biofilms. This study assessed the effects of solutions containing fluoride\/sodium trimetaphosphate (TMP)\/xylitol\/erythritol on dual-species biofilms of Streptococcus mutans and Candida albicans. Biofilms were grown in the continuous presence of these actives alone or in different associations. Quantification of viable plate counts, metabolic activity, biofilm biomass, and extracellular matrix components were evaluated. Overall, fluoride and TMP were the main actives that significantly influenced most of the variables analyzed, with a synergistic effect between them for S. mutans CFUs, biofilm biomass, and protein content of the extracellular matrix (p &lt; 0.05). A similar trend was observed for biofilm metabolic activity and carbohydrate concentrations of the extracellular matrix, although without statistical significance. Regarding the polyols, despite their modest effects on most of the parameters analyzed when administered alone, their co-administration with fluoride and TMP led to a greater reduction in S. mutans CFUs and biofilm biomass compared with fluoride alone at the same concentration. It can be concluded that fluoride and TMP act synergistically on important biofilm parameters, and their co-administration with xylitol\/erythritol significantly impacts S. mutans CFUs and biomass reduction.<\/jats:p>","DOI":"10.3390\/ijms241612910","type":"journal-article","created":{"date-parts":[[2023,8,18]],"date-time":"2023-08-18T10:14:38Z","timestamp":1692353678000},"page":"12910","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Evaluation of Solutions Containing Fluoride, Sodium Trimetaphosphate, Xylitol, and Erythritol, Alone or in Different Associations, on Dual-Species Biofilms"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2524-4365","authenticated-orcid":false,"given":"Igor","family":"Zen","sequence":"first","affiliation":[{"name":"Department of Preventive and Restorative Dentistry, School of Dentistry, Ara\u00e7atuba, S\u00e3o Paulo State University (UNESP), Rua Jos\u00e9 Bonif\u00e1cio, 1193, Ara\u00e7atuba 16015-050, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8159-4853","authenticated-orcid":false,"given":"Alberto Carlos Botazzo","family":"Delbem","sequence":"additional","affiliation":[{"name":"Department of Preventive and Restorative Dentistry, School of Dentistry, Ara\u00e7atuba, S\u00e3o Paulo State University (UNESP), Rua Jos\u00e9 Bonif\u00e1cio, 1193, Ara\u00e7atuba 16015-050, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4153-1548","authenticated-orcid":false,"given":"Tamires Passadori","family":"Martins","sequence":"additional","affiliation":[{"name":"Department of Preventive and Restorative Dentistry, School of Dentistry, Ara\u00e7atuba, S\u00e3o Paulo State University (UNESP), Rua Jos\u00e9 Bonif\u00e1cio, 1193, Ara\u00e7atuba 16015-050, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leonardo Ant\u00f4nio","family":"de Morais","sequence":"additional","affiliation":[{"name":"Department of Preventive and Restorative Dentistry, School of Dentistry, Ara\u00e7atuba, S\u00e3o Paulo State University (UNESP), Rua Jos\u00e9 Bonif\u00e1cio, 1193, Ara\u00e7atuba 16015-050, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caio","family":"Sampaio","sequence":"additional","affiliation":[{"name":"Department of Preventive and Restorative Dentistry, School of Dentistry, Ara\u00e7atuba, S\u00e3o Paulo State University (UNESP), Rua Jos\u00e9 Bonif\u00e1cio, 1193, Ara\u00e7atuba 16015-050, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thayse Yumi","family":"Hosida","sequence":"additional","affiliation":[{"name":"Department of Preventive and Restorative Dentistry, School of Dentistry, Ara\u00e7atuba, S\u00e3o Paulo State University (UNESP), Rua Jos\u00e9 Bonif\u00e1cio, 1193, Ara\u00e7atuba 16015-050, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5229-5259","authenticated-orcid":false,"given":"Douglas Roberto","family":"Monteiro","sequence":"additional","affiliation":[{"name":"Department of Preventive and Restorative Dentistry, School of Dentistry, Ara\u00e7atuba, S\u00e3o Paulo State University (UNESP), Rua Jos\u00e9 Bonif\u00e1cio, 1193, Ara\u00e7atuba 16015-050, SP, Brazil"},{"name":"Postgraduate Program in Health Sciences, University of Western Sa\u00e3o Paulo (UNOESTE), Presidente Prudente 19050-920, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1550-3933","authenticated-orcid":false,"given":"Juliano Pelim","family":"Pessan","sequence":"additional","affiliation":[{"name":"Department of Preventive and Restorative Dentistry, School of Dentistry, Ara\u00e7atuba, S\u00e3o Paulo State University (UNESP), Rua Jos\u00e9 Bonif\u00e1cio, 1193, Ara\u00e7atuba 16015-050, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,8,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"publisher","first-page":"7","DOI":"10.1159\/000503309","article-title":"Terminology of Dental Caries and Dental Caries Management: Consensus Report of a Workshop Organized by ORCA and Cariology Research Group of IADR","volume":"54","author":"Machiulskiene","year":"2020","journal-title":"Caries Res."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Butera, A., Maiorani, C., Morandini, A., Simonini, M., Morittu, S., Trombini, J., and Scribante, A. 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