{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T03:29:43Z","timestamp":1787801383850,"version":"build-2784847793"},"reference-count":65,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2023,8,18]],"date-time":"2023-08-18T00:00:00Z","timestamp":1692316800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Outstanding Youth Science Foundation of Henan Province","award":["232300421038"],"award-info":[{"award-number":["232300421038"]}]},{"name":"Outstanding Youth Science Foundation of Henan Province","award":["Z20221343033"],"award-info":[{"award-number":["Z20221343033"]}]},{"name":"Outstanding Youth Science Foundation of Henan Province","award":["23HASTIT037"],"award-info":[{"award-number":["23HASTIT037"]}]},{"name":"Central Government Guides Local Science and Technology Development Fund","award":["232300421038"],"award-info":[{"award-number":["232300421038"]}]},{"name":"Central Government Guides Local Science and Technology Development Fund","award":["Z20221343033"],"award-info":[{"award-number":["Z20221343033"]}]},{"name":"Central Government Guides Local Science and Technology Development Fund","award":["23HASTIT037"],"award-info":[{"award-number":["23HASTIT037"]}]},{"name":"Program for Science &amp; Technology Innovation Talents in Universities of Henan Province","award":["232300421038"],"award-info":[{"award-number":["232300421038"]}]},{"name":"Program for Science &amp; Technology Innovation Talents in Universities of Henan Province","award":["Z20221343033"],"award-info":[{"award-number":["Z20221343033"]}]},{"name":"Program for Science &amp; Technology Innovation Talents in Universities of Henan Province","award":["23HASTIT037"],"award-info":[{"award-number":["23HASTIT037"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Chlorpyrifos (CPF) and glyphosate (GLY) are the most widely used organophosphate insecticide and herbicide worldwide, respectively; co-occurrence of CPF and GLY in aquatic environments occurs where they inevitably have potential hazards to fish. However, the potential mechanisms of CPF and GLY to induce toxicity have not been fully explored. To identify the adverse impacts of CPF and GLY on fish, either alone or in combination (MIX), CPF (25 \u03bcg\/L) and GLY (3.5 mg\/L) were set up according to an environmentally relevant concentration to expose to common carp for 21 days. After exposure, CPF and GLY decreased the activities of acetylcholinesterase and Na+\/K+-ATPase, altered monoamine oxidase levels, decreased antioxidant enzyme activities (superoxide dismutase, catalase, glutathione S-transferase and glutamic reductase), and induced the accumulation of malondialdehyde in the carp brain. The parameters in the MIX groups had a greater impact compared to that in the CPF or GLY group, suggesting that both single and combined exposure could affect neurological signaling systems and cause oxidative stress and lipid peroxidation damage in carp brains, and that MIX exposure increases the impact of each pollutant. RNA-seq results showed that single or combined exposure to CPF and GLY induced global transcriptomic changes in fish brains, and the number of differentially expressed genes in MIX-treated carp brains were globally increased compared to either the CPF or GLY groups, suggesting that the effects of co-exposure were greater than single exposure. Further analysis results revealed that the global transcriptomic changes participated in oxidative stress, immune dysfunction, and apoptosis of fish brains, and identified that the P13k-Akt signaling pathway participates in both single and combined exposure of CPF- and GLY-induced toxicity. Taken together, our results demonstrated that the interaction of CPF and GLY might be synergic and provided novel insights into the molecular mechanisms of fish brains coping with CPF and GLY.<\/jats:p>","DOI":"10.3390\/ijms241612934","type":"journal-article","created":{"date-parts":[[2023,8,18]],"date-time":"2023-08-18T10:28:48Z","timestamp":1692354528000},"page":"12934","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Single and Combined Effects of Chlorpyrifos and Glyphosate on the Brain of Common Carp: Based on Biochemical and Molecular Perspective"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-7612-0943","authenticated-orcid":false,"given":"Dongfang","family":"Zhang","sequence":"first","affiliation":[{"name":"Henan International Joint Laboratory of Aquatic Toxicology and Health Protection, College of Life Science, Henan Normal University, Xinxiang 453007, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4362-875X","authenticated-orcid":false,"given":"Weikai","family":"Ding","sequence":"additional","affiliation":[{"name":"Henan International Joint Laboratory of Aquatic Toxicology and Health Protection, College of Life Science, Henan Normal University, Xinxiang 453007, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Liu","sequence":"additional","affiliation":[{"name":"Henan International Joint Laboratory of Aquatic Toxicology and Health Protection, College of Life Science, Henan Normal University, Xinxiang 453007, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liuying","family":"Li","sequence":"additional","affiliation":[{"name":"Henan International Joint Laboratory of Aquatic Toxicology and Health Protection, College of Life Science, Henan Normal University, Xinxiang 453007, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gongming","family":"Zhu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Antiviral Drugs, Henan Normal University, Xinxiang 453007, China"},{"name":"Pingyuan Laboratory, Xinxiang 453007, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junguo","family":"Ma","sequence":"additional","affiliation":[{"name":"Henan International Joint Laboratory of Aquatic Toxicology and Health Protection, College of Life Science, Henan Normal University, Xinxiang 453007, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,8,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"134836","DOI":"10.1016\/j.chemosphere.2022.134836","article-title":"Environmental health and risk assessment metrics with special mention to biotransfer, bioaccumulation and biomagnification of environmental pollutants","volume":"302","author":"Chormare","year":"2022","journal-title":"Chemosphere"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"141438","DOI":"10.1016\/j.scitotenv.2020.141438","article-title":"Agrochemical treatments as a source of heavy metals and rare earth elements in agricultural soils and bioaccumulation in ground beetles","volume":"749","author":"Naccarato","year":"2020","journal-title":"Sci. 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