{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T15:50:42Z","timestamp":1785167442892,"version":"3.55.0"},"reference-count":56,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2023,8,29]],"date-time":"2023-08-29T00:00:00Z","timestamp":1693267200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"University of Medicine and Pharmacy \u201cIuliu Ha\u021bieganu\u201d Cluj-Napoca","award":["1300\/5\/12.01.2017"],"award-info":[{"award-number":["1300\/5\/12.01.2017"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Healthcare-associated pneumonia (HCAP) is a common nosocomial infection with high morbidity and mortality. Culture-based detection of the etiologic agent and drug susceptibility is time-consuming, potentially leading to the inadequate use of broad-spectrum empirical antibiotic regimens. The aim was to evaluate the diagnostic capabilities of rapid point-of-care multiplex polymerase chain reaction (PCR) assays from the endotracheal aspirate of critically ill patients with HCAP. A consecutive series of 29 intensive care unit (ICU) patients with HCAP and a control group of 28 patients undergoing elective surgical procedures were enrolled in the study. The results of the PCR assays were compared to the culture-based gold standard. The overall accuracy of the PCR assays was 95.12%, with a sensitivity of 92.31% and a specificity of 97.67%. The median time was 90 min for the rapid PCR tests (p &lt; 0.001), while for the first preliminary results of the cultures, it was 48 h (46\u201372). The overall accuracy for rapid PCR testing in suggesting an adequate antibiotic adjustment was 82.98% (95% CI 69.19\u201392.35%), with a specificity of 90% (95% CI 55.50\u201399.75%), a positive predictive value of 96.77% (95% CI 83.30\u201399.92%), and a negative predictive value of 56.25 (95% CII 29.88\u201380.25%). This method of rapid point-of-care PCR could effectively guide antimicrobial stewardship in patients with healthcare-acquired pneumonia.<\/jats:p>","DOI":"10.3390\/ijms241713393","type":"journal-article","created":{"date-parts":[[2023,8,29]],"date-time":"2023-08-29T08:26:52Z","timestamp":1693297612000},"page":"13393","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Rapid Point-of-Care PCR Testing of Drug-Resistant Strains on Endotracheal Aspirate Samples: A Repurposed Effective Tool in the Stepwise Approach of Healthcare-Acquired Pneumonia\u2014A Pilot Study"],"prefix":"10.3390","volume":"24","author":[{"given":"Andrei-Mihai","family":"B\u0103lan","sequence":"first","affiliation":[{"name":"Department of Anaesthesia and Intensive Care 2, \u201cIuliu Ha\u021bieganu\u201d University of Medicine and Pharmacy Cluj-Napoca, 400012 Cluj-Napoca, Romania"},{"name":"Department of Anaesthesia and Intensive Care, Municipal Clinical Hospital, 400139 Cluj-Napoca, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7798-0762","authenticated-orcid":false,"given":"Constantin","family":"Bodolea","sequence":"additional","affiliation":[{"name":"Department of Anaesthesia and Intensive Care 2, \u201cIuliu Ha\u021bieganu\u201d University of Medicine and Pharmacy Cluj-Napoca, 400012 Cluj-Napoca, Romania"},{"name":"Department of Anaesthesia and Intensive Care, Municipal Clinical Hospital, 400139 Cluj-Napoca, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0600-6097","authenticated-orcid":false,"given":"Andrada","family":"Nemes","sequence":"additional","affiliation":[{"name":"Department of Anaesthesia and Intensive Care 2, \u201cIuliu Ha\u021bieganu\u201d University of Medicine and Pharmacy Cluj-Napoca, 400012 Cluj-Napoca, Romania"},{"name":"Department of Anaesthesia and Intensive Care, Municipal Clinical Hospital, 400139 Cluj-Napoca, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5872-8630","authenticated-orcid":false,"given":"Rare\u0219","family":"Cr\u0103ciun","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, \u201cIuliu Ha\u021bieganu\u201d University of Medicine and Pharmacy Cluj-Napoca, 400012 Cluj-Napoca, Romania"},{"name":"Gastroenterology Clinic, \u201dProf. Dr. O. 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Ref. Module Biomed. Sci., 185\u2013197.","DOI":"10.1016\/B978-0-12-801238-3.11636-8"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"888","DOI":"10.1007\/s00134-020-05980-0","article-title":"Ventilator-Associated Pneumonia in Adults: A Narrative Review","volume":"46","author":"Papazian","year":"2020","journal-title":"Intensive Care Med."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1700582","DOI":"10.1183\/13993003.00582-2017","article-title":"International ERS\/ESICM\/ESCMID\/ALAT Guidelines for the Management of Hospital-Acquired Pneumonia and Ventilator-Associated Pneumonia: Guidelines for the Management of Hospital-Acquired Pneumonia (HAP)\/Ventilator-Associated Pneumonia (VAP) of the European Respiratory Society (ERS), European Society of Intensive Care Medicine (ESICM), European Society of Clinical Microbiology and Infectious Diseases (ESCMID) and Asociaci\u00f3n Latinoamericana Del T\u00f3rax (ALAT)","volume":"50","author":"Torres","year":"2017","journal-title":"Eur. Respir. J."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1016\/j.ccm.2011.06.001","article-title":"Diagnosis of Ventilator-Associated Respiratory Infections (VARI): Microbiologic Clues for Tracheobronchitis (VAT) and Pneumonia (VAP)","volume":"32","author":"Craven","year":"2011","journal-title":"Clin. Chest Med."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"e3325","DOI":"10.1136\/bmj.e3325","article-title":"Ventilator Associated Pneumonia","volume":"344","author":"Hunter","year":"2012","journal-title":"Bmj"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1097\/MCC.0000000000000536","article-title":"Epidemiology of ICU-Acquired Pneumonia","volume":"24","author":"Ferrer","year":"2018","journal-title":"Curr. Opin. Crit. Care"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"488","DOI":"10.1007\/s00134-019-05859-9","article-title":"What Is New in Non-Ventilated ICU-Acquired Pneumonia?","volume":"46","author":"Saied","year":"2020","journal-title":"Intensive Care Med."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1097\/CCM.0000000000003553","article-title":"A Comparison of the Mortality Risk Associated with Ventilator-Acquired Bacterial Pneumonia and Nonventilator ICU-Acquired Bacterial Pneumonia","volume":"47","author":"Saied","year":"2019","journal-title":"Crit. Care Med."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"665","DOI":"10.1016\/S1473-3099(13)70081-1","article-title":"Attributable Mortality of Ventilator-Associated Pneumonia: A Meta-Analysis of Individual Patient Data from Randomised Prevention Studies","volume":"13","author":"Melsen","year":"2013","journal-title":"Lancet Infect. Dis."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1038\/s41572-021-00259-0","article-title":"Pneumonia","volume":"7","author":"Torres","year":"2021","journal-title":"Nat. Rev. Dis. Primer"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1016\/j.ajic.2017.09.005","article-title":"The Epidemiology of Nonventilator Hospital-Acquired Pneumonia in the United States","volume":"46","author":"Giuliano","year":"2018","journal-title":"Am. J. Infect. Control"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"366","DOI":"10.1186\/s13054-020-03067-2","article-title":"Performance and Impact of a Multiplex PCR in ICU Patients with Ventilator-Associated Pneumonia or Ventilated Hospital-Acquired Pneumonia","volume":"24","author":"Bouadma","year":"2020","journal-title":"Crit. Care"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"S81","DOI":"10.1086\/653053","article-title":"Microbial Etiologies of Hospital-Acquired Bacterial Pneumonia and Ventilator-Associated Bacterial Pneumonia","volume":"51","author":"Jones","year":"2010","journal-title":"Clin. Infect. Dis."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1187","DOI":"10.1007\/s00134-017-4682-7","article-title":"The Intensive Care Medicine Research Agenda on Multidrug-Resistant Bacteria, Antibiotics, and Stewardship","volume":"43","author":"Kollef","year":"2017","journal-title":"Intensive Care Med."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"920","DOI":"10.1016\/j.jmii.2019.10.009","article-title":"Performance of a Multiplex PCR Pneumonia Panel for the Identification of Respiratory Pathogens and the Main Determinants of Resistance from the Lower Respiratory Tract Specimens of Adult Patients in Intensive Care Units","volume":"52","author":"Lee","year":"2019","journal-title":"J. Microbiol. Immunol. Infect."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"997","DOI":"10.1136\/thoraxjnl-2013-203384","article-title":"Multidrug-Resistant Pathogens in Hospitalised Patients Coming from the Community with Pneumonia: A European Perspective","volume":"68","author":"Aliberti","year":"2013","journal-title":"Thorax"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"dlac089","DOI":"10.1093\/jacamr\/dlac089","article-title":"Early Appropriate Diagnostics and Treatment of MDR Gram-Negative Infections","volume":"4","author":"Bassetti","year":"2022","journal-title":"JAC-Antimicrob. Resist."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Sfeir, M.M. (2021). Diagnosis of Multidrug-Resistant Pathogens of Pneumonia. Diagnostics, 11.","DOI":"10.3390\/diagnostics11122287"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"334","DOI":"10.4066\/AMJ.2014.2105","article-title":"Ventilator-Associated Pneumonia","volume":"7","author":"Charles","year":"2014","journal-title":"Australas. Med. J."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1056\/NEJMoa1500245","article-title":"Community-Acquired Pneumonia Requiring Hospitalization among US Adults","volume":"373","author":"Jain","year":"2015","journal-title":"N. Engl. J. Med."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1046","DOI":"10.1126\/science.280.5366.1046","article-title":"Chemical Amplification: Continuous-Flow PCR on a Chip","volume":"280","author":"Kopp","year":"1998","journal-title":"Science"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1039\/b605957k","article-title":"Disposable Real-Time MicroPCR Device: Lab-on-a-Chip at a Low Cost","volume":"2","author":"Neuzil","year":"2006","journal-title":"Mol. Biosyst."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1764","DOI":"10.1183\/13993003.01144-2016","article-title":"Laboratory Diagnosis of Pneumonia in the Molecular Age","volume":"48","author":"Torres","year":"2016","journal-title":"Eur. Respir. J."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/j.clinmicnews.2008.11.001","article-title":"GeneXpert Testing: Applications for Clinical Microbiology, Part I","volume":"30","author":"Marlowe","year":"2008","journal-title":"Clin. Microbiol. Newsl."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1186\/s12941-017-0191-3","article-title":"Systematic Review and Meta-Analysis of Mortality of Patients Infected with Carbapenem-Resistant Klebsiella Pneumoniae","volume":"16","author":"Xu","year":"2017","journal-title":"Ann. Clin. Microbiol. Antimicrob."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1007\/s15010-021-01643-4","article-title":"Multidrug-Resistant Acinetobacter Baumannii Infections in COVID-19 Patients Hospitalized in Intensive Care Unit","volume":"50","author":"Russo","year":"2022","journal-title":"Infection"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"\u010ciginskien\u0117, A., Dambrauskien\u0117, A., Rello, J., and Adukauskien\u0117, D. (2019). Ventilator-Associated Pneumonia Due to Drug-Resistant Acinetobacter Baumannii: Risk Factors and Mortality Relation with Resistance Profiles, and Independent Predictors of in-Hospital Mortality. Medicina, 55.","DOI":"10.3390\/medicina55020049"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"e02349-16","DOI":"10.1128\/AAC.02349-16","article-title":"Multicenter Clinical and Molecular Epidemiological Analysis of Bacteremia Due to Carbapenem-Resistant Enterobacteriaceae (CRE) in the CRE Epicenter of the United States","volume":"61","author":"Satlin","year":"2017","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"2066","DOI":"10.1093\/cid\/ciac354","article-title":"Impact of a Rapid Molecular Test for Klebsiella Pneumoniae Carbapenemase and Ceftazidime-Avibactam Use on Outcomes after Bacteremia Caused by Carbapenem-Resistant Enterobacterales","volume":"75","author":"Satlin","year":"2022","journal-title":"Clin. Infect. Dis."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1128\/JCM.01679-15","article-title":"Evaluation of the FilmArray Blood Culture Identification Panel: Results of a Multicenter Controlled Trial","volume":"54","author":"Salimnia","year":"2016","journal-title":"J. Clin. Microbiol."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Tojo, M., Fujita, T., Ainoda, Y., Nagamatsu, M., Hayakawa, K., Mezaki, K., Sakurai, A., Masui, Y., Yazaki, H., and Takahashi, H. (2014). Evaluation of an Automated Rapid Diagnostic Assay for Detection of Gram-Negative Bacteria and Their Drug-Resistance Genes in Positive Blood Cultures. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0094064"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Li, Z. (2022). The Value of GeneXpert MTB\/RIF for Detection in Tuberculosis: A Bibliometrics-Based Analysis and Review. J. Anal. Methods Chem., 2915018.","DOI":"10.1155\/2022\/2915018"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"4138","DOI":"10.1128\/JCM.05434-11","article-title":"Evaluation of the GeneXpert MTB\/RIF Assay for Rapid Diagnosis of Tuberculosis and Detection of Rifampin Resistance in Pulmonary and Extrapulmonary Specimens","volume":"49","author":"Zeka","year":"2011","journal-title":"J. Clin. Microbiol."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Evans, C.A. (2011). GeneXpert\u2014A Game-Changer for Tuberculosis Control?. PLoS Med., 8.","DOI":"10.1371\/journal.pmed.1001064"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"3832","DOI":"10.1128\/AAC.00517-16","article-title":"Improvement of the Xpert Carba-R Kit for the Detection of Carbapenemase-Producing Enterobacteriaceae","volume":"60","author":"Dortet","year":"2016","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Cortegiani, A., Russotto, V., Graziano, G., Geraci, D., Saporito, L., Cocorullo, G., Raineri, S.M., Mammina, C., and Giarratano, A. (2016). Use of Cepheid Xpert Carba-R\u00ae for Rapid Detection of Carbapenemase-Producing Bacteria in Abdominal Septic Patients Admitted to Intensive Care Unit. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0160643"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"E23410","DOI":"10.1097\/MD.0000000000023410","article-title":"Xpert Carba-R Assay for Detection of Carbapenemase-Producing Organisms in Patients Admitted to Emergency Rooms","volume":"99","author":"Jin","year":"2020","journal-title":"Medicine"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1286.e9","DOI":"10.1016\/j.cmi.2019.03.003","article-title":"Comparison of Five Methods for Detection of Carbapenemases in Enterobacterales with Proposal of a New Algorithm","volume":"25","author":"Baeza","year":"2019","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/j.eimc.2016.06.009","article-title":"Estudio de Coste-Efectividad Del Diagn\u00f3stico Microbiol\u00f3gico de Tuberculosis Mediante GeneXpert MTB\/RIF\u00ae","volume":"35","year":"2017","journal-title":"Enferm. Infecc. Microbiol. Clin."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"e059823","DOI":"10.1136\/bmjopen-2021-059823","article-title":"Cost-Effectiveness of GeneXpert Omni Compared with GeneXpert MTB\/Rif for Point-of-Care Diagnosis of Tuberculosis in a Low-Resource, High-Burden Setting in Eastern Uganda: A Cost-Effectiveness Analysis Based on Decision Analytical Modelling","volume":"12","author":"Ejalu","year":"2022","journal-title":"BMJ Open"},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Kaso, A.W., and Hailu, A. (2021). Costs and Cost-Effectiveness of Gene Xpert Compared to Smear Microscopy for the Diagnosis of Pulmonary Tuberculosis Using Real-World Data from Arsi Zone, Ethiopia. PLoS ONE, 16.","DOI":"10.1371\/journal.pone.0259056"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"e61","DOI":"10.1093\/cid\/ciw353","article-title":"Management of Adults with Hospital-Acquired and Ventilator-Associated Pneumonia: 2016 Clinical Practice Guidelines by the Infectious Diseases Society of America and the American Thoracic Society","volume":"63","author":"Kalil","year":"2016","journal-title":"Clin. Infect. Dis."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"e00015-22","DOI":"10.1128\/cmr.00015-22","article-title":"The Microbial Etiology of Community-Acquired Pneumonia in Adults: From Classical Bacteriology to Host Transcriptional Signatures","volume":"35","author":"Gadsby","year":"2022","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_45","first-page":"1019","article-title":"Bacteriology of Expectorated Sputum with Quantitative Culture and Wash Technique Compared to Transtracheal Aspirates","volume":"117","author":"Bartlett","year":"1978","journal-title":"Am. Rev. Respir. Dis."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"791","DOI":"10.1183\/09031936.01.17407910","article-title":"Torres Microbial Investigation in Ventilator-Associated Pneumonia","volume":"17","author":"Ioanas","year":"2001","journal-title":"Eur. Respir. J."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Schytz, H.W., and Hvedstrup, J. (2023). Evaluating Headache and Facial Pain in a Headache Diagnostic Laboratory: Experiences from the Danish Headache Center. Diagnostics, 13.","DOI":"10.3390\/diagnostics13162671"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"552","DOI":"10.1099\/jmm.0.001171","article-title":"Rapid Molecular Testing for Staphylococcus Aureus Bacteraemia Improves Clinical Management","volume":"69","author":"McHugh","year":"2020","journal-title":"J. Med. Microbiol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"823","DOI":"10.1128\/JCM.01884-08","article-title":"Rapid Detection of Staphylococcus Aureus and Methicillin-Resistant S. Aureus (MRSA) in Wound Specimens and Blood Cultures: Multicenter Preclinical Evaluation of the Cepheid Xpert MRSA\/SA Skin and Soft Tissue and Blood Culture Assays","volume":"47","author":"Wolk","year":"2009","journal-title":"J. Clin. Microbiol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"690","DOI":"10.1309\/AJCP07UGYOKBVVNC","article-title":"Validation and Implementation of the GeneXpert MRSA\/SA Blood Culture Assay in a Pediatric Setting","volume":"136","author":"Spencer","year":"2011","journal-title":"Am. J. Clin. Pathol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"995","DOI":"10.1007\/s10096-010-0958-3","article-title":"Evaluation of the Xpert MRSA Assay for Rapid Detection of Methicillin-Resistant Staphylococcus Aureus from Nares Swabs of Geriatric Hospitalized Patients and Failure to Detect a Specific SCC Mec Type IV Variant","volume":"29","author":"Laurent","year":"2010","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1276","DOI":"10.1128\/JB.01128-07","article-title":"Gene Acquisition at the Insertion Site for SCC Mec, the Genomic Island Conferring Methicillin Resistance in Staphylococcus Aureus","volume":"190","author":"Noto","year":"2008","journal-title":"J. Bacteriol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"2011","DOI":"10.1128\/JCM.00232-07","article-title":"Evaluation of Three Molecular Assays for Rapid Identification of Methicillin-Resistant Staphylococcus Aureus","volume":"45","author":"Francois","year":"2007","journal-title":"J. Clin. Microbiol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1007\/s10096-011-1306-y","article-title":"Evaluation of GeneOhm VanR and Xpert VanA\/VanB Molecular Assays for the Rapid Detection of Vancomycin-Resistant Enterococci","volume":"31","author":"Gazin","year":"2012","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1008","DOI":"10.3906\/sag-1301-54","article-title":"Evaluation of GeneXpert VanA\/VanB Assay for the Detection of Vancomycin-Resistant Enterococci in Patients Newly Admitted to Intensive Care Units","volume":"43","author":"Altun","year":"2013","journal-title":"Turk. J. Med. Sci."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1111\/j.1469-0691.2012.03821.x","article-title":"Rapid Evolution and Spread of Carbapenemases among Enterobacteriaceae in Europe","volume":"18","author":"Carmeli","year":"2012","journal-title":"Clin. Microbiol. 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