{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T21:32:11Z","timestamp":1780522331911,"version":"3.54.1"},"reference-count":54,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2023,8,30]],"date-time":"2023-08-30T00:00:00Z","timestamp":1693353600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Research Foundation of the State Key Laboratory of Food Science and Resources, Nanchang University, China","award":["SKLF-ZZB-202328"],"award-info":[{"award-number":["SKLF-ZZB-202328"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Cyclophosphamide causes side effects in cancer patients, including hepatotoxicity. Probiotics have recently emerged as potential approaches for the administration of many diseases. This study aimed to evaluate the protective effects of Lactiplantibacillus plantarum P101 against cyclophosphamide-induced liver injury and elucidate the underlying mechanism. In this study, Lactiplantibacillus plantarum P101 or Lactobacillus rhamnosus GG were pre-administered to mice with varying duration (1 week, 2 weeks, and 3 weeks) before being intraperitoneally injected with cyclophosphamide at a dose of 30 mg\/kg\/day for 7 days to induce liver injury. Results demonstrated that cyclophosphamide-induced liver injury was characterized by histopathological disorders, including irregular central venous shape and hepatic vascular rupture, as well as a severe inflammation response and oxidative stress. The administration of probiotics for 3 weeks exerted the most significant improvements in alleviating liver injury, oxidative stress, and inflammation when compared to the shorter intervention duration. Notably, Lactiplantibacillus\u00a0plantarum P101 exhibited more pronounced effects than Lactobacillus rhamnosus GG. Furthermore, Lactiplantibacillus plantarum P101 enhanced the antioxidant defense system by activating the Nrf2\/ARE signaling pathway, ultimately alleviating hepatotoxicity and hepatocyte apoptosis. In conclusion, this study highlighted the potential of Lactiplantibacillus plantarum P101 to alleviate cyclophosphamide-induced hepatotoxicity.<\/jats:p>","DOI":"10.3390\/ijms241713424","type":"journal-article","created":{"date-parts":[[2023,8,30]],"date-time":"2023-08-30T09:44:46Z","timestamp":1693388686000},"page":"13424","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Lactiplantibacillus plantarum P101 Attenuated Cyclophosphamide-Induced Liver Injury in Mice by Regulating the Nrf2\/ARE Signaling Pathway"],"prefix":"10.3390","volume":"24","author":[{"given":"Tao","family":"You","sequence":"first","affiliation":[{"name":"State Key Laboratory of Food Science and Resources, Nanchang University, 235 Nanjing East Road, Nanchang 330047, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Zhao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Food Science and Resources, Nanchang University, 235 Nanjing East Road, Nanchang 330047, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shanji","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Food Science and Resources, Nanchang University, 235 Nanjing East Road, Nanchang 330047, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0457-9665","authenticated-orcid":false,"given":"Hengyi","family":"Xu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Food Science and Resources, Nanchang University, 235 Nanjing East Road, Nanchang 330047, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,8,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"839464","DOI":"10.3389\/fphar.2022.839464","article-title":"Cyclophosphamide Induces the Ferroptosis of Tumor Cells through Heme Oxygenase-1","volume":"13","author":"Shi","year":"2022","journal-title":"Front. Pharmacol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"888960","DOI":"10.3389\/fnut.2022.888960","article-title":"Protective Effect of Oyster Peptides Derived From Crassostrea gigas on Intestinal Oxidative Damage Induced by Cyclophosphamide in Mice Mediated Through Nrf2-Keap1 Signaling Pathway","volume":"9","author":"Chen","year":"2022","journal-title":"Front. Nutr."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"120590","DOI":"10.1016\/j.lfs.2022.120590","article-title":"Trimetazidine attenuates cyclophosphamide-induced cystitis by inhibiting TLR4-mediated NF\u03baB signaling in mice","volume":"301","author":"Engin","year":"2022","journal-title":"Life Sci."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"30383","DOI":"10.1007\/s11356-021-17990-6","article-title":"Pyrroloquinoline quinone ameliorates liver injury in mice induced by cyclophosphamide","volume":"29","author":"Qian","year":"2022","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Aladaileh, S.H., Abukhalil, M.H., Saghir, S.A.M., Hanieh, H., Alfwuaires, M.A., Almaiman, A.A., Bin-Jumah, M., and Mahmoud, A.M. (2019). Galangin Activates Nrf2 Signaling and Attenuates Oxidative Damage, Inflammation, and Apoptosis in a Rat Model of Cyclophosphamide-Induced Hepatotoxicity. Biomolecules, 9.","DOI":"10.3390\/biom9080346"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1016\/j.etap.2016.05.031","article-title":"Punicalagin alleviates hepatotoxicity in rats challenged with cyclophosphamide","volume":"45","author":"Fouad","year":"2016","journal-title":"Environ. Toxicol. Pharmacol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1016\/j.biopha.2016.12.047","article-title":"Possible involvement of Nrf2 and PPAR\u03b3 up-regulation in the protective effect of umbelliferone against cyclophosphamide-induced hepatotoxicity","volume":"86","author":"Mahmoud","year":"2017","journal-title":"Biomed. Pharmacother."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"634","DOI":"10.1016\/j.biopha.2018.08.039","article-title":"Premna integrifolia ameliorates cyclophosphamide-induced hepatotoxicity by modulation of oxidative stress and apoptosis","volume":"107","author":"Singh","year":"2018","journal-title":"Biomed. Pharmacother."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"R1147","DOI":"10.1016\/j.cub.2017.09.019","article-title":"The liver","volume":"27","author":"Trefts","year":"2017","journal-title":"Curr. Biol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1039\/C6FO01531J","article-title":"The protective effects of Cichorium glandulosum seed and cynarin against cyclophosphamide and its metabolite acrolein-induced hepatotoxicity in vivo and in vitro","volume":"8","author":"Tong","year":"2017","journal-title":"Food Funct."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"112266","DOI":"10.1016\/j.fct.2021.112266","article-title":"Naringin prevents cyclophosphamide-induced hepatotoxicity in rats by attenuating oxidative stress, fibrosis, and inflammation","volume":"153","author":"Akamo","year":"2021","journal-title":"Food Chem. Toxicol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"2007","DOI":"10.3109\/13880209.2015.1137954","article-title":"Efficacy of Rosmarinus officinalis leaves extract against cyclophosphamide-induced hepatotoxicity","volume":"54","author":"Germoush","year":"2016","journal-title":"Pharm. Biol."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Ghareeb, M.A., Sobeh, M., El-Maadawy, W.H., Mohammed, H.S., Khalil, H., Botros, S., and Wink, M. (2019). Chemical Profiling of Polyphenolics in Eucalyptus globulus and Evaluation of Its Hepato\u2013Renal Protective Potential Against Cyclophosphamide Induced Toxicity in Mice. Antioxidants, 8.","DOI":"10.3390\/antiox8090415"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1186\/s40168-019-0761-z","article-title":"Ochratoxin A induces liver inflammation: Involvement of intestinal microbiota","volume":"7","author":"Wang","year":"2019","journal-title":"Microbiome"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1038\/s41575-018-0099-1","article-title":"Alcohol, liver disease and the gut microbiota","volume":"16","author":"Bajaj","year":"2019","journal-title":"Nat. Rev. Gastroenterol. Hepatol."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Kanmani, P., Suganya, K., and Kim, H. (2020). The Gut Microbiota: How Does It Influence the Development and Progression of Liver Diseases. Biomedicines, 8.","DOI":"10.3390\/biomedicines8110501"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1016\/j.jhep.2018.10.019","article-title":"Microbiome as a therapeutic target in alcohol-related liver disease","volume":"70","author":"Sarin","year":"2019","journal-title":"J. Hepatol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"111393","DOI":"10.1016\/j.ecoenv.2020.111393","article-title":"Restraining the TiO2 nanoparticles-induced intestinal inflammation mediated by gut microbiota in juvenile rats via ingestion of Lactobacillus rhamnosus GG","volume":"206","author":"Zhao","year":"2020","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/j.tifs.2019.11.022","article-title":"Interactions between probiotics and pathogenic microorganisms in hosts and foods: A review","volume":"95","author":"Abhari","year":"2020","journal-title":"Trends Food Sci. Technol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"638148","DOI":"10.3389\/fonc.2021.638148","article-title":"Probiotics in Cancer","volume":"11","author":"Lu","year":"2021","journal-title":"Front. Oncol."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Wang, Y., Wu, Y., Wang, Y., Xu, H., Mei, X., Yu, D., Wang, Y., and Li, W. (2017). Antioxidant Properties of Probiotic Bacteria. Nutrients, 9.","DOI":"10.3390\/nu9050521"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"3216","DOI":"10.1039\/C4FO00549J","article-title":"Lactobacillus plantarum NCU116 improves liver function, oxidative stress and lipid metabolism in rats with high fat diet induced non-alcoholic fatty liver disease","volume":"5","author":"Li","year":"2014","journal-title":"Food Funct."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Wu, Y., Wang, B., Tang, L., Zhou, Y., Wang, Q., Gong, L., Ni, J., and Li, W. (2022). Probiotic Bacillus Alleviates Oxidative Stress-Induced Liver Injury by Modulating Gut-Liver Axis in a Rat Model. Antioxidants, 11.","DOI":"10.3390\/antiox11020291"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"111533","DOI":"10.1016\/j.fct.2020.111533","article-title":"Lactobacillus plantarum LP33 attenuates Pb-induced hepatic injury in rats by reducing oxidative stress and inflammation and promoting Pb excretion","volume":"143","author":"Hu","year":"2020","journal-title":"Food Chem. Toxicol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"982","DOI":"10.1039\/c3fo30316k","article-title":"Antioxidant effects of Lactobacillus plantarum via activation of transcription factor Nrf2","volume":"4","author":"Gao","year":"2013","journal-title":"Food Funct."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"e22558","DOI":"10.1002\/jbt.22558","article-title":"Nicotinamide attenuates cyclophosphamide-induced hepatotoxicity in SD rats by reducing oxidative stress and apoptosis","volume":"34","author":"Patwa","year":"2020","journal-title":"J. Biochem. Mol. Toxicol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.freeradbiomed.2017.05.006","article-title":"Cyclophosphamide and acrolein induced oxidative stress leading to deterioration of metaphase II mouse oocyte quality","volume":"110","author":"Jeelani","year":"2017","journal-title":"Free Radic. Biol. Med."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"849","DOI":"10.1016\/j.dld.2007.06.001","article-title":"Endotoxin- and D-galactosamine-induced liver injury improved by the administration of Lactobacillus, Bifidobacterium and blueberry","volume":"39","author":"Osman","year":"2007","journal-title":"Dig. Liver Dis."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1599","DOI":"10.1007\/s10753-014-9886-1","article-title":"Protective Effects of Lactobacillus plantarum NDC 75017 against Lipopolysaccharide-Induced Liver Injury in Mice","volume":"37","author":"Peng","year":"2014","journal-title":"Inflammation"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"963","DOI":"10.1002\/biof.1679","article-title":"Nerolidol protects the liver against cyclophosphamide-induced hepatic inflammation, apoptosis, and fibrosis via modulation of Nrf2, NF-\u03baB p65, and caspase-3 signaling molecules in Swiss albino mice","volume":"46","author":"Iqubal","year":"2020","journal-title":"BioFactors"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"104057","DOI":"10.1016\/j.jff.2020.104057","article-title":"Effects of polysaccharides from bee collected pollen of Chinese wolfberry on immune response and gut microbiota composition in cyclophosphamide-treated mice","volume":"72","author":"Zhao","year":"2020","journal-title":"J. Funct. Foods"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"104085","DOI":"10.1016\/j.jff.2020.104085","article-title":"Ameliorative effect of low molecular weight peptides from the head of red shrimp (Solenocera crassicornis) against cyclophosphamide-induced hepatotoxicity in mice","volume":"72","author":"Jiang","year":"2020","journal-title":"J. Funct. Foods"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1007\/s12263-015-0491-1","article-title":"18\u03b2-Glycyrrhetinic acid exerts protective effects against cyclophosphamide-induced hepatotoxicity: Potential role of PPAR\u03b3 and Nrf2 upregulation","volume":"10","author":"Mahmoud","year":"2015","journal-title":"Genes Nutr."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"717","DOI":"10.1139\/cjpp-2014-0204","article-title":"Hesperidin protects against cyclophosphamide-induced hepatotoxicity by upregulation of PPAR\u03b3 and abrogation of oxidative stress and inflammation","volume":"92","author":"Mahmoud","year":"2014","journal-title":"Can. J. Physiol. Pharmacol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"e22271","DOI":"10.1002\/jbt.22271","article-title":"Ganoderic acid A against cyclophosphamide-induced hepatic toxicity in mice","volume":"33","author":"Xu","year":"2019","journal-title":"J. Biochem. Mol. Toxicol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/j.ctim.2019.08.005","article-title":"Panax ginseng is superior to vitamin E as a hepatoprotector against cyclophosphamide-induced liver damage","volume":"46","author":"Shalaby","year":"2019","journal-title":"Complement. Ther. Med."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1180","DOI":"10.1080\/01480545.2020.1810262","article-title":"Escin attenuates oxidative damage, apoptosis and lipid peroxidation in a model of cyclophosphamide-induced liver damage","volume":"45","author":"Cengiz","year":"2022","journal-title":"Drug Chem. Toxicol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.jff.2014.07.008","article-title":"Blueberry anthocyanins ameliorate cyclophosphamide-induced liver damage in rats by reducing inflammation and apoptosis","volume":"11","author":"Shi","year":"2014","journal-title":"J. Funct. Foods"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"20968","DOI":"10.1007\/s11356-018-2242-5","article-title":"Naringin protects against cyclophosphamide-induced hepatotoxicity and nephrotoxicity through modulation of oxidative stress, inflammation, apoptosis, autophagy, and DNA damage","volume":"25","author":"Caglayan","year":"2018","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"8597","DOI":"10.1007\/s00253-019-10122-8","article-title":"Protective effects of Lactobacillus plantarum against chronic alcohol-induced liver injury in the murine model","volume":"103","author":"Fang","year":"2019","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1089\/jmf.2018.4357","article-title":"Lactobacillus rhamnosus Granules Dose-Dependently Balance Intestinal Microbiome Disorders and Ameliorate Chronic Alcohol-Induced Liver Injury","volume":"23","author":"Gu","year":"2019","journal-title":"J. Med. Food"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.intimp.2012.10.026","article-title":"Protective effects of probiotic Lactobacillus casei Zhang against endotoxin- and D-galactosamine-induced liver injury in rats via anti-oxidative and anti-inflammatory capacities","volume":"15","author":"Wang","year":"2013","journal-title":"Int. Immunopharmacol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1016\/j.foodchem.2009.05.014","article-title":"Hepatoprotective effect of the root extract of Decalepis hamiltonii against carbon tetrachloride-induced oxidative stress in rats","volume":"118","author":"Srivastava","year":"2010","journal-title":"Food Chem."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1291","DOI":"10.1021\/acs.jafc.5b06177","article-title":"Lactobacillus plantarum NCU116 Attenuates Cyclophosphamide-Induced Immunosuppression and Regulates Th17\/Treg Cell Immune Responses in Mice","volume":"64","author":"Xie","year":"2016","journal-title":"J. Agric. Food. Chem."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"533","DOI":"10.3109\/09637486.2015.1024204","article-title":"Effect of Lactobacillus plantarum NCU116 on loperamide-induced constipation in mice","volume":"66","author":"Li","year":"2015","journal-title":"Int. J. Food Sci. Nutr."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"731","DOI":"10.3389\/fmicb.2019.00731","article-title":"Lactobacillus plantarum KLDS1.0318 Ameliorates Impaired Intestinal Immunity and Metabolic Disorders in Cyclophosphamide-Treated Mice","volume":"10","author":"Meng","year":"2019","journal-title":"Front. Microbiol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/j.yexmp.2010.10.013","article-title":"Proteasome inhibitor up regulates liver antioxidative enzymes in rat model of alcoholic liver disease","volume":"90","author":"Oliva","year":"2011","journal-title":"Exp. Mol. Pathol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"39625","DOI":"10.1007\/s11356-021-13392-w","article-title":"Allicin mitigates hepatic injury following cyclophosphamide administration via activation of Nrf2\/ARE pathways and through inhibition of inflammatory and apoptotic machinery","volume":"28","author":"Sun","year":"2021","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"153083","DOI":"10.1016\/j.tox.2021.153083","article-title":"Toxicity mechanism of acrolein on DNA damage and apoptosis in BEAS-2B cells: Insights from cell biology and molecular docking analyses","volume":"466","author":"Liu","year":"2022","journal-title":"Toxicology"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"skaa077","DOI":"10.1093\/jas\/skaa077","article-title":"Illicium verum extracts and probiotics with added glucose oxidase promote antioxidant capacity through upregulating hepatic and jejunal Nrf2\/Keap1 of weaned piglets","volume":"98","author":"Zhang","year":"2020","journal-title":"J. Anim. Sci."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"9632","DOI":"10.1039\/D1FO00277E","article-title":"Exopolysaccharides produced by Lactobacillus rhamnosus GG alleviate hydrogen peroxide-induced intestinal oxidative damage and apoptosis through the Keap1\/Nrf2 and Bax\/Bcl-2 pathways in vitro","volume":"12","author":"Li","year":"2021","journal-title":"Food Funct."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"3015","DOI":"10.1007\/s00253-016-8032-4","article-title":"Bacillus amyloliquefaciens SC06 alleviates the oxidative stress of IPEC-1 via modulating Nrf2\/Keap1 signaling pathway and decreasing ROS production","volume":"101","author":"Wang","year":"2017","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Chen, X., Zhang, J., Yi, R., Mu, J., Zhao, X., and Yang, Z. (2018). Hepatoprotective Effects of Lactobacillus on Carbon Tetrachloride-Induced Acute Liver Injury in Mice. Int. J. Mol. Sci., 19.","DOI":"10.3390\/ijms19082212"},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Zhao, Y., Tang, Y., Liu, S., Jia, T., Zhou, D., and Xu, H. (2021). Foodborne TiO2 Nanoparticles Induced More Severe Hepatotoxicity in Fructose-Induced Metabolic Syndrome Mice via Exacerbating Oxidative Stress-Mediated Intestinal Barrier Damage. Foods, 10.","DOI":"10.3390\/foods10050986"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/17\/13424\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T20:42:38Z","timestamp":1760128958000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/17\/13424"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,8,30]]},"references-count":54,"journal-issue":{"issue":"17","published-online":{"date-parts":[[2023,9]]}},"alternative-id":["ijms241713424"],"URL":"https:\/\/doi.org\/10.3390\/ijms241713424","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,8,30]]}}}