{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,26]],"date-time":"2026-07-26T02:45:08Z","timestamp":1785033908360,"version":"3.55.0"},"reference-count":67,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2023,8,30]],"date-time":"2023-08-30T00:00:00Z","timestamp":1693353600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Russian Science Foundation","award":["21-14-00178"],"award-info":[{"award-number":["21-14-00178"]}]},{"name":"Ministry of Science and Higher Education of the Russian Federation","award":["21-14-00178"],"award-info":[{"award-number":["21-14-00178"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>\u03b1H-Crystallin, a high molecular weight form of \u03b1-crystallin, is one of the major proteins in the lens nucleus. This high molecular weight aggregate (HMWA) plays an important role in the pathogenesis of cataracts. We have shown that the chaperone-like activity of HMWA is 40% of that of \u03b1-crystallin from the lens cortex. Refolding with urea significantly increased\u2014up to 260%\u2014the chaperone-like activity of \u03b1-crystallin and slightly reduced its hydrodynamic diameter (Dh). HMWA refolding resulted in an increase in chaperone-like activity up to 120% and a significant reduction of Dh of protein particles compared with that of \u03b1-crystallin. It was shown that the chaperone-like activity of HMWA, \u03b1-crystallin, and refolded \u03b1-crystallin but not refolded HMWA was strongly correlated with the denaturation enthalpy measured with differential scanning calorimetry (DSC). The DSC data demonstrated a significant increase in the native protein portion of refolded \u03b1-crystallin in comparison with authentic \u03b1-crystallin; however, the denaturation enthalpy of refolded HMWA was significantly decreased in comparison with authentic HMWA. The authors suggested that the increase in the chaperone-like activity of both \u03b1-crystallin and HMWA could be the result of the correction of misfolded proteins during renaturation and the rearrangement of protein supramolecular structures.<\/jats:p>","DOI":"10.3390\/ijms241713473","type":"journal-article","created":{"date-parts":[[2023,8,30]],"date-time":"2023-08-30T09:44:46Z","timestamp":1693388686000},"page":"13473","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Refolding Increases the Chaperone-like Activity of \u03b1H-Crystallin and Reduces Its Hydrodynamic Diameter to That of \u03b1-Crystallin"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8280-5926","authenticated-orcid":false,"given":"Konstantin O.","family":"Muranov","sequence":"first","affiliation":[{"name":"Emanuel Institute of Biochemical Physics of Russian Academy of Sciences, Moscow 119334, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolay B.","family":"Poliansky","sequence":"additional","affiliation":[{"name":"Emanuel Institute of Biochemical Physics of Russian Academy of Sciences, Moscow 119334, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vera A.","family":"Borzova","sequence":"additional","affiliation":[{"name":"Federal Research Centre \u201cFundamentals of Biotechnology\u201d of Russian Academy of Sciences, Bach Institute of Biochemistry, Moscow 119334, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5085-0327","authenticated-orcid":false,"given":"Sergey Y.","family":"Kleimenov","sequence":"additional","affiliation":[{"name":"Koltzov Institute of Developmental Biology of Russian Academy of Sciences, Moscow 119334, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,8,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"728","DOI":"10.2471\/BLT.12.104034","article-title":"Global cost of correcting vision impairment from uncorrected refractive error","volume":"90","author":"Fricke","year":"2012","journal-title":"Bull. 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