{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,14]],"date-time":"2026-02-14T05:44:25Z","timestamp":1771047865222,"version":"3.50.1"},"reference-count":52,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2023,9,2]],"date-time":"2023-09-02T00:00:00Z","timestamp":1693612800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000050","name":"NHLBI","doi-asserted-by":"publisher","award":["1K01HL155240"],"award-info":[{"award-number":["1K01HL155240"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000050","name":"NHLBI","doi-asserted-by":"publisher","award":["KL2TR002492"],"award-info":[{"award-number":["KL2TR002492"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000050","name":"NHLBI","doi-asserted-by":"publisher","award":["UL1TR002494"],"award-info":[{"award-number":["UL1TR002494"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000050","name":"NHLBI","doi-asserted-by":"publisher","award":["19IPLOI34760288"],"award-info":[{"award-number":["19IPLOI34760288"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006108","name":"NCATS","doi-asserted-by":"publisher","award":["1K01HL155240"],"award-info":[{"award-number":["1K01HL155240"]}],"id":[{"id":"10.13039\/100006108","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006108","name":"NCATS","doi-asserted-by":"publisher","award":["KL2TR002492"],"award-info":[{"award-number":["KL2TR002492"]}],"id":[{"id":"10.13039\/100006108","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006108","name":"NCATS","doi-asserted-by":"publisher","award":["UL1TR002494"],"award-info":[{"award-number":["UL1TR002494"]}],"id":[{"id":"10.13039\/100006108","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006108","name":"NCATS","doi-asserted-by":"publisher","award":["19IPLOI34760288"],"award-info":[{"award-number":["19IPLOI34760288"]}],"id":[{"id":"10.13039\/100006108","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000968","name":"American Heart Association","doi-asserted-by":"publisher","award":["1K01HL155240"],"award-info":[{"award-number":["1K01HL155240"]}],"id":[{"id":"10.13039\/100000968","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000968","name":"American Heart Association","doi-asserted-by":"publisher","award":["KL2TR002492"],"award-info":[{"award-number":["KL2TR002492"]}],"id":[{"id":"10.13039\/100000968","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000968","name":"American Heart Association","doi-asserted-by":"publisher","award":["UL1TR002494"],"award-info":[{"award-number":["UL1TR002494"]}],"id":[{"id":"10.13039\/100000968","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000968","name":"American Heart Association","doi-asserted-by":"publisher","award":["19IPLOI34760288"],"award-info":[{"award-number":["19IPLOI34760288"]}],"id":[{"id":"10.13039\/100000968","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Preeclampsia is the leading cause of maternal\u2013fetal morbidity worldwide. The concept that persistent feto-placental intolerance is important in the pathogenesis of preeclampsia (PreE) has been demonstrated by our lab and others. Arginine vasopressin (AVP) infusion during pregnancy induces cardiovascular, renal, and T helper (TH) cell alterations in mice consistent with human PreE. In addition to their conventional immuno-stimulatory role, dendritic cells (DCs) also play a vital role in immune tolerance. In contrast to conventional DCs, regulatory DCs (DCregs) express low levels of co-stimulatory markers, produce anti-inflammatory cytokines, induce T regulatory (Treg) cells, and promote tolerance. In mice, DCregs prevent pro-inflammatory responses and induce antigen-specific tolerance. Given these known functions of DCregs, we hypothesize that DCregs will prevent the development of AVP-induced PreE in mice. C57BL\/6J females were infused with AVP (24 ng\/h) or saline throughout gestation via an osmotic minipump. Bone-marrow-derived DCregs were injected into AVP-infused dams at the time of the pump implantation or on gestational day (GD) 7. The blood pressure of the mice was taken throughout their pregnancy. The maternal urine proteins and TH-associated cytokines in maternal and fetal tissues were measured on GD 18. The treatment with DCregs effectively prevented the elevation of maternal blood pressure, proteinuria, and fetal growth restriction that were observed in AVP-infused dams. Furthermore, we noted a reduction in the pro-inflammatory TH-associated cytokines IFN\u03b3 and IL-17, while anti-inflammatory cytokines IL-4, IL-10, and TGF\u03b2 showed an increase following DCreg treatment. These outcomes provide strong evidence supporting the potential of DCregs as a valuable therapeutic approach in addressing PreE.<\/jats:p>","DOI":"10.3390\/ijms241713594","type":"journal-article","created":{"date-parts":[[2023,9,4]],"date-time":"2023-09-04T02:14:50Z","timestamp":1693793690000},"page":"13594","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Cellular Immunotherapy in Mice Prevents Maternal Hypertension and Restores Anti-Inflammatory Cytokine Balance in Maternal and Fetal Tissues"],"prefix":"10.3390","volume":"24","author":[{"given":"Gabrielle","family":"Gray","sequence":"first","affiliation":[{"name":"Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Douglas G.","family":"Scroggins","sequence":"additional","affiliation":[{"name":"School of Medicine, Department of Biomedical Sciences, University of Minnesota Duluth, Duluth, MN 55812, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katlin T.","family":"Wilson","sequence":"additional","affiliation":[{"name":"School of Medicine, Department of Biomedical Sciences, University of Minnesota Duluth, Duluth, MN 55812, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5842-0575","authenticated-orcid":false,"given":"Sabrina M.","family":"Scroggins","sequence":"additional","affiliation":[{"name":"School of Medicine, Department of Biomedical Sciences, University of Minnesota Duluth, Duluth, MN 55812, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,9,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"921","DOI":"10.1016\/j.ajog.2005.10.813","article-title":"Preeclampsia, gestational hypertension and intrauterine growth restriction, related or independent conditions?","volume":"194","author":"Villar","year":"2006","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"(2020). Gestational Hypertension and Preeclampsia: ACOG Practice Bulletin, Number 222. Obs. Gynecol., 135, e237\u2013e260.","DOI":"10.1097\/AOG.0000000000003891"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"e2018741","DOI":"10.1001\/jamanetworkopen.2020.18741","article-title":"Evaluation of US State-Level Variation in Hypertensive Disorders of Pregnancy","volume":"3","author":"Butwick","year":"2020","journal-title":"JAMA Netw. Open."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1176","DOI":"10.1161\/STROKEAHA.108.538025","article-title":"Pre-eclampsia is associated with increased risk of stroke in the adult offspring: The Helsinki birth cohort study","volume":"40","author":"Kajantie","year":"2009","journal-title":"Stroke A J. Cereb. Circ."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"944","DOI":"10.1161\/HYPERTENSIONAHA.109.130765","article-title":"Hypertensive pregnancy disorders and subsequent cardiovascular morbidity and type 2 diabetes mellitus in the mother","volume":"53","author":"Lykke","year":"2009","journal-title":"Hypertension"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"269.e1","DOI":"10.1016\/j.ajog.2009.06.060","article-title":"Health of children born to mothers who had preeclampsia: A population-based cohort study","volume":"201","author":"Wu","year":"2009","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"961","DOI":"10.1097\/AOG.0b013e3181bb0dfc","article-title":"Hypertensive disorders in pregnancy and subsequently measured cardiovascular risk factors","volume":"114","author":"Magnussen","year":"2009","journal-title":"Obs. Gynecol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1299","DOI":"10.1097\/AOG.0b013e3181a45b25","article-title":"Hypertensive disorders and severe obstetric morbidity in the United States","volume":"113","author":"Kuklina","year":"2009","journal-title":"Obs. Gynecol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1080\/01674820802546212","article-title":"Cognitive function after pre-eclampsia: An explorative study","volume":"30","author":"Baecke","year":"2009","journal-title":"J. Psychosom. Obs. Gynaecol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"408","DOI":"10.1080\/00016340801915127","article-title":"Impaired maternal cognitive functioning after pregnancies complicated by severe pre-eclampsia: A pilot case-control study","volume":"87","author":"Brusse","year":"2008","journal-title":"Acta Obstet. Et. Gynecol. Scand."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"74.e1","DOI":"10.1016\/j.ajog.2017.03.008","article-title":"Preeclampsia and cognitive impairment later in life","volume":"217","author":"Fields","year":"2017","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"37.e1","DOI":"10.1016\/j.ajog.2014.01.042","article-title":"Neurocognitive functioning following preeclampsia and eclampsia: A long-term follow-up study","volume":"211","author":"Postma","year":"2014","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1016\/j.preghy.2013.06.003","article-title":"The brain study: Cognition, quality of life and social functioning following preeclampsia; An observational study","volume":"3","author":"Postma","year":"2013","journal-title":"Pregnancy Hypertens."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"685","DOI":"10.1055\/s-0030-1253099","article-title":"Neurocognitive functioning in women with a history of eclampsia: Executive functioning and sustained attention","volume":"27","author":"Postma","year":"2010","journal-title":"Am. J. Perinatol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1080\/14767050500127740","article-title":"Physical well-being in women with a history of severe preeclampsia","volume":"18","author":"Roes","year":"2005","journal-title":"J. Matern. Fetal Neonatal Med."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"889918","DOI":"10.3389\/fphys.2022.889918","article-title":"Experimental Preeclampsia Causes Long-Lasting Hippocampal Vascular Dysfunction and Memory Impairment","volume":"13","author":"Johnson","year":"2022","journal-title":"Front. Physiol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"e072157","DOI":"10.1136\/bmj-2022-072157","article-title":"Maternal hypertensive disorder of pregnancy and mortality in offspring from birth to young adulthood: National population based cohort study","volume":"379","author":"Huang","year":"2022","journal-title":"BMJ"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"984291","DOI":"10.3389\/fmed.2022.984291","article-title":"The Global Pregnancy Collaboration (CoLab) symposium on short- and long-term outcomes in offspring whose mothers had preeclampsia: A scoping review of clinical evidence","volume":"9","author":"Korzeniewski","year":"2022","journal-title":"Front. Med."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1358\/dnp.2009.22.9.1437961","article-title":"From molecules to medicine: A future cure for preeclampsia?","volume":"22","author":"Santillan","year":"2009","journal-title":"Drug. News Perspect."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1016\/j.ajog.2005.07.035","article-title":"Divergent trophoblast invasion and apoptosis in placental bed spiral arteries from pregnancies complicated by maternal anemia and early-onset preeclampsia\/intrauterine growth restriction","volume":"194","author":"Kadyrov","year":"2006","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.jri.2012.01.006","article-title":"The predominance of Th17 lymphocytes and decreased number and function of Treg cells in preeclampsia","volume":"93","author":"Tabarkiewicz","year":"2012","journal-title":"J. Reprod. Immunol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"949","DOI":"10.1161\/HYPERTENSIONAHA.110.168344","article-title":"CD4+ T-helper cells stimulated in response to placental ischemia mediate hypertension during pregnancy","volume":"57","author":"Wallace","year":"2011","journal-title":"Hypertension"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"510","DOI":"10.1093\/humupd\/dmq007","article-title":"An immunological insight into the origins of pre-eclampsia","volume":"16","author":"Olson","year":"2010","journal-title":"Hum. Reprod. Update"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"534","DOI":"10.1111\/j.1600-0897.2010.00831.x","article-title":"Immunology of pre-eclampsia","volume":"63","author":"Redman","year":"2010","journal-title":"Am. J. Reprod. Immunol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1042\/CS20171059","article-title":"Elevated vasopressin in pregnant mice induces T-helper subset alterations consistent with human preeclampsia","volume":"132","author":"Scroggins","year":"2018","journal-title":"Clin. Sci."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1038\/nri2335","article-title":"Dendritic cells in intestinal immune regulation","volume":"8","author":"Coombes","year":"2008","journal-title":"Nat. Rev. Immunol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"767","DOI":"10.1146\/annurev.immunol.18.1.767","article-title":"Immunobiology of dendritic cells","volume":"18","author":"Banchereau","year":"2000","journal-title":"Annu. Rev. Immunol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1038\/nri3254","article-title":"Cross-presentation by dendritic cells","volume":"12","author":"Joffre","year":"2012","journal-title":"Nat. Rev. Immunol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"610","DOI":"10.1038\/nri2132","article-title":"Tolerogenic dendritic cells and the quest for transplant tolerance","volume":"7","author":"Morelli","year":"2007","journal-title":"Nat. Rev. Immunol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.imlet.2004.04.015","article-title":"Regulatory T cells and tolerogenic dendritic cells: From basic biology to clinical applications","volume":"94","author":"Rutella","year":"2004","journal-title":"Immunol. Lett."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"685","DOI":"10.1146\/annurev.immunol.21.120601.141040","article-title":"Tolerogenic dendritic cells","volume":"21","author":"Steinman","year":"2003","journal-title":"Annu. Rev. Immunol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"5088","DOI":"10.1182\/blood-2011-11-364091","article-title":"Dendritic cells and regulation of graft-versus-host disease and graft-versus-leukemia activity","volume":"119","author":"Stenger","year":"2012","journal-title":"Blood"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1365","DOI":"10.1189\/jlb.0307166","article-title":"Balancing between immunity and tolerance: An interplay between dendritic cells, regulatory T cells, and effector T cells","volume":"82","author":"Cools","year":"2007","journal-title":"J. Leukoc. Biol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1765","DOI":"10.4049\/jimmunol.0902133","article-title":"Tolerogenic dendritic cells generated with different immunosuppressive cytokines induce antigen-specific anergy and regulatory properties in memory CD4+ T Cells","volume":"184","author":"Munguia","year":"2010","journal-title":"J. Immunol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"104128","DOI":"10.1016\/j.jri.2023.104128","article-title":"Characterization of progesterone-induced dendritic cells in metabolic and immunologic reprogramming","volume":"159","author":"Liu","year":"2023","journal-title":"J. Reprod. Immunol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1075","DOI":"10.1016\/j.cmet.2019.11.011","article-title":"Human Tolerogenic Dendritic Cells Regulate Immune Responses through Lactate Synthesis","volume":"30","author":"Marin","year":"2019","journal-title":"Cell. Metab."},{"key":"ref_37","first-page":"599623","article-title":"Vitamin D3-Induced Tolerogenic Dendritic Cells Modulate the Transcriptomic Profile of T CD4(+) Cells towards a Functional Hyporesponsiveness","volume":"11","author":"Mansilla","year":"2020","journal-title":"Front. Immunol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"3656","DOI":"10.1182\/blood-2005-10-4190","article-title":"Regulatory dendritic cells act as regulators of acute lethal systemic inflammatory response","volume":"107","author":"Fujita","year":"2006","journal-title":"Blood"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/j.jaci.2007.08.038","article-title":"Regulatory dendritic cells protect against allergic airway inflammation in a murine asthmatic model","volume":"121","author":"Fujita","year":"2008","journal-title":"J. Allergy Clin. Immunol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"5868","DOI":"10.4049\/jimmunol.177.9.5868","article-title":"\u201cAlternatively activated\u201d dendritic cells preferentially secrete IL-10, expand Foxp3+CD4+ T cells, and induce long-term organ allograft survival in combination with CTLA4-Ig","volume":"177","author":"Lan","year":"2006","journal-title":"J. Immunol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"3787","DOI":"10.1182\/blood-2005-11-4495","article-title":"Vasoactive intestinal peptide induces regulatory dendritic cells that prevent acute graft-versus-host disease while maintaining the graft-versus-tumor response","volume":"107","author":"Chorny","year":"2006","journal-title":"Blood"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1016\/S1074-7613(03)00055-4","article-title":"Regulatory dendritic cells protect mice from murine acute graft-versus-host disease and leukemia relapse","volume":"18","author":"Sato","year":"2003","journal-title":"Immunity"},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Scroggins, S.M., Olivier, A.K., Meyerholz, D.K., and Schlueter, A.J. (2013). Characterization of regulatory dendritic cells that mitigate acute graft-versus-host disease in older mice following allogeneic bone marrow transplantation. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0075158"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1980","DOI":"10.1002\/eji.202250068","article-title":"Tolerogenic dendritic cells alleviate collagen-induced arthritis by forming microchimerism and affecting the expression of immune checkpoint molecules","volume":"52","author":"Wan","year":"2022","journal-title":"Eur. J. Immunol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"13562","DOI":"10.1073\/pnas.0504484102","article-title":"Vasoactive intestinal peptide induces regulatory dendritic cells with therapeutic effects on autoimmune disorders","volume":"102","author":"Chorny","year":"2005","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"e99403","DOI":"10.1172\/jci.insight.99403","article-title":"Arginine vasopressin infusion is sufficient to model clinical features of preeclampsia in mice","volume":"3","author":"Sandgren","year":"2018","journal-title":"JCI Insight"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"852","DOI":"10.1161\/HYPERTENSIONAHA.114.03848","article-title":"Vasopressin in preeclampsia: A novel very early human pregnancy biomarker and clinically relevant mouse model","volume":"64","author":"Santillan","year":"2014","journal-title":"Hypertension"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1080\/19396368.2021.1981486","article-title":"Physiological characterization of an arginine vasopressin rat model of preeclampsia","volume":"68","author":"Ramdin","year":"2022","journal-title":"Syst. Biol. Reprod. Med."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"710","DOI":"10.1038\/cmi.2017.70","article-title":"The altered PD-1\/PD-L1 pathway delivers the \u2018one-two punch\u2019 effects to promote the Treg\/Th17 imbalance in pre-eclampsia","volume":"15","author":"Zhang","year":"2018","journal-title":"Cell. Mol. Immunol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1016\/j.clim.2008.01.013","article-title":"Generation of therapeutic dendritic cells and regulatory T cells for preventing allogeneic cardiac graft rejection","volume":"127","author":"Zhang","year":"2008","journal-title":"Clin. Immunol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"R818","DOI":"10.1152\/ajpregu.00082.2013","article-title":"Hypertension in mice with transgenic activation of the brain renin-angiotensin system is vasopressin dependent","volume":"304","author":"Littlejohn","year":"2013","journal-title":"Am. J. Physiol. Regul. Integr. Comp. Physiol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"e1225","DOI":"10.14814\/phy2.12257","article-title":"Pregnant mice lacking indoleamine 2,3-dioxygenase exhibit preeclampsia phenotypes","volume":"3","author":"Santillan","year":"2015","journal-title":"Physiol. Rep."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/17\/13594\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T20:45:24Z","timestamp":1760129124000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/17\/13594"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,9,2]]},"references-count":52,"journal-issue":{"issue":"17","published-online":{"date-parts":[[2023,9]]}},"alternative-id":["ijms241713594"],"URL":"https:\/\/doi.org\/10.3390\/ijms241713594","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,9,2]]}}}