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Recently, many efforts have been made to load metals into bioactive glasses to enhance the multifunctionality of materials, such as antibacterial and osteoinductive functions. Zinc has been documented to stimulate the gene expression of various regulatory factors in bone cells. Meanwhile, previous studies have reported that silver and zinc could be a promising antibacterial combination with synergistic antimicrobial effects. Here, we sought to develop a biomaterial coreleasing zinc and silver, designated 80S-ZnAg, and to evaluate its antibacterial activity and biocompatibility. The textural analyses demonstrated different coreleasing patterns of zinc and silver for the materials. The chemical characterization revealed that the zinc in 80S-ZnAg could be the network modifier when its molar ratio was high, releasing more zinc; zinc could also be the network former when its molar ratio was low, showing an extremely low rate of release. However, the ICP results for 80S-Zn3Ag2 demonstrated up to 7.5 ppm of zinc and 67.6 ppm of silver. Among all the 80S-ZnAg materials, 80S-Zn3Ag2 demonstrated more marked antibacterial activity against MRSA and VRE than the others, with inhibition zones of 11.5 and 13.4 mm, respectively. The cytotoxicity assay exhibited nearly 90% cell viability at 20 mg\/mL of 80-Zn3Ag2. Further clinical study is needed to develop an innovative biomaterial to address the issue of antibiotic resistance.<\/jats:p>","DOI":"10.3390\/ijms241813679","type":"journal-article","created":{"date-parts":[[2023,9,5]],"date-time":"2023-09-05T09:46:20Z","timestamp":1693907180000},"page":"13679","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Synthesis, Characterization, and Bioactivity of Mesoporous Bioactive Glass Codoped with Zinc and Silver"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8579-0299","authenticated-orcid":false,"given":"Tsung-Ying","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Medical Laboratory Science, I-Shou University, Kaohsiung 84001, Taiwan"},{"name":"Research Organization for Nano and Life Innovation, Future Innovation Institute, Waseda University, Tokyo 162-0041, Japan"},{"name":"Research Institute for Science and Engineering, Waseda University, Tokyo 169-8555, Japan"},{"name":"School of Education, Waseda University, Tokyo 169-8050, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guann-In","family":"Chern","sequence":"additional","affiliation":[{"name":"Department of Fragrance and Cosmetic Science, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 80708, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei-Hsun","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery, Changhua Christian Hospital, Changhua 50006, Taiwan"},{"name":"Department of Post-Baccalaureate Medicine, College of Medicine, National Chung Hsing University, Taichung 40227, Taiwan"},{"name":"School of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan"},{"name":"Department of Chemical Engineering, National United University, Miaoli 36063, Taiwan"},{"name":"Department of Golden-Ager Industry Management, Chaoyang University of Technology, Taichung 41349, Taiwan"},{"name":"Department of Medical Imaging and Radiology, Shu-Zen Junior College of Medicine and Management, Kaohsiung 82144, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1776-6139","authenticated-orcid":false,"given":"Chi-Jen","family":"Shih","sequence":"additional","affiliation":[{"name":"Department of Fragrance and Cosmetic Science, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 80708, Taiwan"},{"name":"Drug Development and Value Creation Research Center, Kaohsiung Medical University, Kaohsiung 80708, Taiwan"},{"name":"Department of Medical Research, Kaohsiung Medical University Hospital, Kaohsiung 80708, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,9,5]]},"reference":[{"key":"ref_1","first-page":"272","article-title":"Review of contemporary knowledge of osteomyelitis diagnosis","volume":"92","author":"Michno","year":"2018","journal-title":"World Sci. 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