{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T03:58:32Z","timestamp":1788580712624,"version":"build-2803163510"},"reference-count":116,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2023,9,6]],"date-time":"2023-09-06T00:00:00Z","timestamp":1693958400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Science and Technology Council, Taiwan, R.O.C.","award":["MOST 111WFA1610040"],"award-info":[{"award-number":["MOST 111WFA1610040"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Rhizobacteria from various ecological niches display variations in physiological characteristics. This study investigates the transcriptome profiling of two Bacillus subtilis strains, BsCP1 and BsPG1, each isolated from distinct environments. Gene expression linked to the synthesis of seven types of antibiotic compounds was detected in both BsCP1 and BsPG1 cultures. Among these, the genes associated with plipastatin synthesis were predominantly expressed in both bacterial strains. However, genes responsible for the synthesis of polyketide, subtilosin, and surfactin showed distinct transcriptional patterns. Additionally, genes involved in producing exopolysaccharides (EPS) showed higher expression levels in BsPG1 than in BsCP1. Consistently with this, a greater quantity of EPS was found in the BsPG1 culture compared to BsCP1. Both bacterial strains exhibited similar effects on Arabidopsis seedlings, promoting root branching and increasing seedling fresh weight. However, BsPG1 was a more potent enhancer of drought, heat, and copper stress tolerance than BsCP1. Treatment with BsPG1 had a greater impact on improving survival rates, increasing starch accumulation, and stabilizing chlorophyll content during the post-stress stage. qPCR analysis was used to measure transcriptional changes in Arabidopsis seedlings in response to BsCP1 and BsPG1 treatment. The results show that both bacterial strains had a similar impact on the expression of genes involved in the salicylic acid (SA) and jasmonic acid (JA) signaling pathways. Likewise, genes associated with stress response, root development, and disease resistance showed comparable responses to both bacterial strains. However, treatment with BsCP1 and BsPG1 induced distinct activation of genes associated with the ABA signaling pathway. The results of this study demonstrate that bacterial strains from different ecological environments have varying abilities to produce beneficial metabolites for plant growth. Apart from the SA and JA signaling pathways, ABA signaling triggered by PGPR bacterial strains could play a crucial role in building an effective resistance to various abiotic stresses in the plants they colonize.<\/jats:p>","DOI":"10.3390\/ijms241813720","type":"journal-article","created":{"date-parts":[[2023,9,6]],"date-time":"2023-09-06T10:07:37Z","timestamp":1693994857000},"page":"13720","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Examining the Transcriptomic and Biochemical Signatures of Bacillus subtilis Strains: Impacts on Plant Growth and Abiotic Stress Tolerance"],"prefix":"10.3390","volume":"24","author":[{"given":"Peter E.","family":"Chang","sequence":"first","affiliation":[{"name":"Department of Agricultural Biotechnology, National Chiayi University, Chiayi 600355, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun-Hsiang","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Agricultural Biotechnology, National Chiayi University, Chiayi 600355, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ciao-Yun","family":"Tai","sequence":"additional","affiliation":[{"name":"Department of Agricultural Biotechnology, National Chiayi University, Chiayi 600355, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I-Hung","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Agricultural Biotechnology, National Chiayi University, Chiayi 600355, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2647-2658","authenticated-orcid":false,"given":"Wen-Der","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Agricultural Biotechnology, National Chiayi University, Chiayi 600355, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tong-Seung","family":"Tseng","sequence":"additional","affiliation":[{"name":"Department of Agricultural Biotechnology, National Chiayi University, Chiayi 600355, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huey-wen","family":"Chuang","sequence":"additional","affiliation":[{"name":"Department of Agricultural Biotechnology, National Chiayi University, Chiayi 600355, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,9,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1111\/j.1574-6976.2007.00072.x","article-title":"Indole-3-acetic acid in microbial and microorganism-plant signaling","volume":"31","author":"Spaepen","year":"2007","journal-title":"FEMS Microbiol. Rev."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"871","DOI":"10.1007\/s11103-005-4547-2","article-title":"Lateral root initiation or the birth of a new meristem","volume":"60","author":"Vanneste","year":"2006","journal-title":"Plant. Mol. Biol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1186\/s40538-022-00370-8","article-title":"Total auxin level in the soil\u2013plant system as a modulating factor for the effectiveness of PGPR inocula: A review","volume":"10","year":"2023","journal-title":"Chem. Biol. Technol. Agric."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Nithyapriya, S., Lalitha, S., Sayyed, R.Z., Reddy, M.S., Dailin, D.J., El Enshasy, H.A., Luh Suriani, N., and Herlambang, S. (2021). Production, purification, and characterization of bacillibactin siderophore of Bacillus subtilis and its application for improvement in plant growth and oil content in sesame. Sustainability, 13.","DOI":"10.3390\/su13105394"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1992","DOI":"10.1111\/ppl.13545","article-title":"The ACC deaminase-producing plant growth-promoting bacteria: Influences of bacterial strains and ACC deaminase activities in plant tolerance to abiotic stress","volume":"173","author":"Naing","year":"2021","journal-title":"Physiol. Plant."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/j.micres.2018.01.002","article-title":"Microbial volatiles as plant rogwth inducers","volume":"208","author":"Fincheira","year":"2018","journal-title":"Microbiol. Res."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1016\/j.mib.2018.02.011","article-title":"Healthy scents: Microbial volatiles as new frontier in antibiotic research?","volume":"45","author":"Avalos","year":"2018","journal-title":"Curr. Opin. Microbiol."},{"key":"ref_8","first-page":"5625","article-title":"Acetoin and 2,3-butanediol from Bacillus amyloliquefaciens induce stomatal closure in Arabidopsis thaliana and Nicotiana benthamiana","volume":"69","author":"Wu","year":"2018","journal-title":"J. Exp. Bot."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1016\/j.plaphy.2018.06.010","article-title":"2, 3-Butanediol activated disease-resistance of creeping bentgrass by inducing phytohormone and antioxidant responses","volume":"129","author":"Shi","year":"2018","journal-title":"Plant Physiol. Biochem."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1037","DOI":"10.1111\/j.1574-6976.2010.00221.x","article-title":"Natural functions of lipopeptides from Bacillus and Pseudomonas: More than surfactants and antibiotics","volume":"34","author":"Raaijmakers","year":"2010","journal-title":"FEMS Microbiol. Rev."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"456","DOI":"10.1094\/MPMI-22-4-0456","article-title":"Insights into the defense-related events occurring in plant cells following perception of surfactin-type lipopeptide from Bacillus subtilis","volume":"22","author":"Jourdan","year":"2009","journal-title":"Mol. Plant Microbe Interact."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1038\/nrmicro.2016.94","article-title":"Biofilms: An emergent form of bacterial life","volume":"14","author":"Flemming","year":"2016","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Ilyas, N., Mumtaz, K., Akhtar, N., Yasmin, H., Sayyed, R.Z., Khan, W., Enshasy, H.A.E., Dailin, D.J., Elsayed, E.A., and Ali, Z. (2020). Exopolysaccharides producing bacteria for the amelioration of drought stress in wheat. Sustainability, 12.","DOI":"10.3390\/su12218876"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Morcillo, R.J.L., and Manzanera, M. (2021). The effects of plant-associated bacterial exopolysaccharides on plant abiotic stress tolerance. Metabolites, 11.","DOI":"10.3390\/metabo11060337"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"356","DOI":"10.3389\/fpls.2013.00356","article-title":"Plant growth-promoting rhizobacteria and root system functioning","volume":"4","author":"Vacheron","year":"2013","journal-title":"Front. Plant Sci."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Yang, X., Lu, M., Wang, Y., Wang, Y., Liu, Z., and Chen, S. (2021). Response mechanism of plants to drought stress. Horticulturae, 7.","DOI":"10.20944\/preprints202102.0466.v1"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1016\/j.envexpbot.2010.05.011","article-title":"How plants sense temperature","volume":"69","author":"Ruelland","year":"2010","journal-title":"Environ. Exp. Bot."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"985","DOI":"10.1007\/s11738-012-1169-6","article-title":"Heavy metal-induced oxidative damage, defense reactions, and detoxification mechanisms in plants","volume":"35","author":"Sytar","year":"2013","journal-title":"Acta Physiol. Plant."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Hasanuzzaman, M., Nahar, K., Hossain, S.M., Mahmud, J.A., Rahman, A., Inafuku, M., Oku, H., and Fujita, M. (2017). Coordinated actions of glyoxalase and antioxidant defense systems in conferring abiotic stress tolerance in plants. Int. J. Mol. Sci., 18.","DOI":"10.3390\/ijms18010200"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1111\/j.1365-313X.2007.03234.x","article-title":"A hydraulic signal in root-to-shoot signalling of water shortage","volume":"52","author":"Christmann","year":"2007","journal-title":"Plant J."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1807","DOI":"10.1104\/pp.17.01010","article-title":"Abscisic acid-induced reactive oxygen species are modulated by flavonols to control stomata aperture","volume":"175","author":"Watkins","year":"2017","journal-title":"Plant Physiol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1234","DOI":"10.1111\/nph.16953","article-title":"Systemic propagation of immunity in plants","volume":"229","author":"Vlot","year":"2021","journal-title":"New Phytol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"100603","DOI":"10.1016\/j.rhisph.2022.100603","article-title":"Alleviation of salinity induced growth and photosynthetic decline in wheat due to biochar and jasmonic acid application involves up-regulation of ascorbate-glutathione pathway, glyoxylase system and secondary metabolite accumulation","volume":"24","author":"Alharbi","year":"2022","journal-title":"Rhizosphere"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1007\/s40003-020-00490-3","article-title":"Salicylic acid and hydrogen peroxide improve antioxidant response and compatible osmolytes in wheat (Triticum aestivum L.) under water deficit","volume":"10","author":"Singh","year":"2021","journal-title":"Agric. Res."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Wang, L.-J., Fan, L., Loescher, W., Duan, W., Liu, G.-J., Cheng, J.-S., Luo, H.-B., and Li, S.-H. (2010). Salicylic acid alleviates decreases in photosynthesis under heat stress and accelerates recovery in grapevine leaves. BMC Plant Biol., 10.","DOI":"10.1186\/1471-2229-10-34"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"e26374","DOI":"10.4161\/psb.26374","article-title":"Salicylic acid alleviates adverse effects of heat stress on photosynthesis through changes in proline production and ethylene formation","volume":"8","author":"Khan","year":"2013","journal-title":"Plant Signal. Behav."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"664519","DOI":"10.3389\/fpls.2021.664519","article-title":"Exogenous methyl jasmonate improves heat tolerance of perennial ryegrass through alteration of osmotic adjustment, antioxidant defense, and expression of jasmonic acid-responsive genes","volume":"12","author":"Su","year":"2021","journal-title":"Front. Plant Sci."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1017","DOI":"10.1104\/pp.103.026583","article-title":"Bacterial volatiles induce systemic resistance in Arabidopsis","volume":"134","author":"Ryu","year":"2004","journal-title":"Plant Physiol."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Le Mire, G., Siah, A., Brisset, M.-N., Gaucher, M., Deleu, M., and Jijakli, M.H. (2018). Surfactin protects wheat against Zymoseptoria tritici and activates both salicylic acid-and jasmonic acid-dependent defense responses. Agriculture, 8.","DOI":"10.3390\/agriculture8010011"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1715","DOI":"10.1007\/s10529-013-1286-3","article-title":"A wider role for polyamines in biofilm formation","volume":"35","author":"Karatan","year":"2013","journal-title":"Biotechnol. Lett."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Zhou, C., Ma, Z., Zhu, L., Xiao, X., Xie, Y., Zhu, J., and Wang, J. (2016). Rhizobacterial strain Bacillus megaterium BOFC15 induces cellular polyamine changes that improve plant growth and drought resistance. Int. J. Mol. Sci., 17.","DOI":"10.3390\/ijms17060976"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Gomez, M.Y., Schroeder, M.M., Chieb, M., McLain, N.K., and Gachomo, E.W. (2023). Bradyrhizobium japonicum IRAT FA3 promotes salt tolerance through jasmonic acid priming in Arabidopsis thaliana. BMC Plant Biol., 23.","DOI":"10.1186\/s12870-022-03977-z"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Tsai, S.-H., Hsiao, Y.-C., Chang, P.E., Kuo, C.-E., Lai, M.-C., and Chuang, H.-w. (2023). Exploring the biologically active metabolites produced by Bacillus cereus for plant growth promotion, heat stress tolerance, and resistance to bacterial soft rot in Arabidopsis. Metabolites, 13.","DOI":"10.3390\/metabo13050676"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1111\/1365-2745.13630","article-title":"Root functional traits explain root exudation rate and composition across a range of grassland species","volume":"110","author":"Williams","year":"2022","journal-title":"J. Ecol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"e00770-20","DOI":"10.1128\/mSystems.00770-20","article-title":"Genomic and chemical diversity of Bacillus subtilis secondary metabolites against plant pathogenic fungi","volume":"6","author":"Kiesewalter","year":"2021","journal-title":"Msystems"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"7209","DOI":"10.1074\/jbc.M009140200","article-title":"The dhb operon of Bacillus subtilis encodes the biosynthetic template for the catecholic siderophore 2, 3-dihydroxybenzoate-glycine-threonine trimeric ester bacillibactin","volume":"276","author":"May","year":"2001","journal-title":"J. Biol. Chem."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"6823","DOI":"10.1093\/emboj\/18.23.6823","article-title":"Crystal structure of the surfactin synthetase-activating enzyme Sfp: A prototype of the 4\u2032-phosphopantetheinyl transferase superfamily","volume":"18","author":"Reuter","year":"1999","journal-title":"EMBO J."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.jbiotec.2018.07.044","article-title":"Biological control of plant pathogens by Bacillus species","volume":"285","author":"Fira","year":"2018","journal-title":"J. Biotechnol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1094\/PHYTO-96-0653","article-title":"Fusarium wilt of banana is caused by several pathogens referred to as Fusarium oxysporum f. sp","volume":"96","author":"Ploetz","year":"2006","journal-title":"cubense. Phytopathology"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1016\/S0734-9750(99)00014-2","article-title":"Phosphate solubilizing bacteria and their role in plant growth promotion","volume":"17","author":"Fraga","year":"1999","journal-title":"Biotechnol. Adv."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/j.envexpbot.2011.11.004","article-title":"Water-stress-induced thermotolerance of photosynthesis in bean (Phaseolus vulgaris L.) plants: The possible involvement of lipid composition and xanthophyll cycle pigments","volume":"77","author":"Pastenes","year":"2012","journal-title":"Environ. Exp. Bot."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1399","DOI":"10.1111\/j.1365-3040.2008.01853.x","article-title":"Relationship between carbohydrate partitioning and drought resistance in common bean","volume":"31","author":"Covarrubias","year":"2008","journal-title":"Plant Cell Environ."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"5211","DOI":"10.1007\/s11356-019-07264-7","article-title":"Copper-induced oxidative stress, initiation of antioxidants and phytoremediation potential of flax (Linum usitatissimum L.) seedlings grown under the mixing of two different soils of China","volume":"27","author":"Saleem","year":"2020","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Angulo-Bejarano, P.I., Puente-Rivera, J., and Cruz-Ortega, R. (2021). Metal and metalloid toxicity in plants: An overview on molecular aspects. Plants, 10.","DOI":"10.3390\/plants10040635"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1279","DOI":"10.1104\/pp.108.119420","article-title":"Characterization and biological function of the ISOCHORISMATE SYNTHASE2 gene of Arabidopsis","volume":"147","author":"Garcion","year":"2008","journal-title":"Plant Physiol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"3292","DOI":"10.1073\/pnas.96.6.3292","article-title":"EDS1, an essential component of R gene-mediated disease resistance in Arabidopsis has homology to eukaryotic lipases","volume":"96","author":"Falk","year":"1999","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Wang, L., Tsuda, K., Sato, M., Cohen, J.D., Katagiri, F., and Glazebrook, J. (2009). Arabidopsis CaM binding protein CBP60g contributes to MAMP-induced SA accumulation and is involved in disease resistance against Pseudomonas syringae. PLoS Pathog., 5.","DOI":"10.1371\/journal.ppat.1000301"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"8675","DOI":"10.1073\/pnas.92.19.8675","article-title":"A chloroplast lipoxygenase is required for wound-induced jasmonic acid accumulation in Arabidopsis","volume":"92","author":"Bell","year":"1995","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"692","DOI":"10.1007\/s00425-002-0909-z","article-title":"Cloning and characterization of a jasmonic acid-responsive gene encoding 12-oxophytodienoic acid reductase in suspension-cultured rice cells","volume":"216","author":"Sobajima","year":"2003","journal-title":"Planta"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1577","DOI":"10.1101\/gad.297704","article-title":"Conserved MYC transcription factors play a key role in jasmonate signaling both in tomato and Arabidopsis","volume":"18","author":"Boter","year":"2004","journal-title":"Genes Dev."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1046\/j.1365-313X.2003.01786.x","article-title":"Molecular characterization of the Arabidopsis 9-cis epoxycarotenoid dioxygenase gene family","volume":"35","author":"Tan","year":"2003","journal-title":"Plant J."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1016\/j.cj.2020.06.005","article-title":"OsVP1 activates Sdr4 expression to control rice seed dormancy via the ABA signaling pathway","volume":"9","author":"Chen","year":"2021","journal-title":"Crop J."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1007\/s10265-011-0412-3","article-title":"ABA-mediated transcriptional regulation in response to osmotic stress in plants","volume":"24","author":"Fujita","year":"2011","journal-title":"J. Plant Res."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"18822","DOI":"10.1073\/pnas.0605639103","article-title":"Dual function of an Arabidopsis transcription factor DREB2A in water-stress-responsive and heat-stress-responsive gene expression","volume":"103","author":"Sakuma","year":"2006","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"436","DOI":"10.1104\/pp.106.078717","article-title":"Transcriptomic footprints disclose specificity of reactive oxygen species signaling in Arabidopsis","volume":"141","author":"Gadjev","year":"2006","journal-title":"Plant Physiol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"847","DOI":"10.1104\/pp.105.068254","article-title":"The zinc-finger protein Zat12 plays a central role in reactive oxygen and abiotic stress signaling in Arabidopsis","volume":"139","author":"Davletova","year":"2005","journal-title":"Plant Physiol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1105\/tpc.104.026971","article-title":"Cytosolic ascorbate peroxidase 1 is a central component of the reactive oxygen gene network of Arabidopsis","volume":"17","author":"Davletova","year":"2005","journal-title":"Plant Cell"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1016\/0092-8674(94)90544-4","article-title":"H2O2 from the oxidative burst orchestrates the plant hypersensitive disease resistance response","volume":"79","author":"Levine","year":"1994","journal-title":"Cell"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"2079","DOI":"10.31018\/jans.v9i4.1492","article-title":"Osmolytes: Proline metabolism in plants as sensors of abiotic stress","volume":"9","author":"Singh","year":"2017","journal-title":"J. Appl. Nat. Sci."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1046\/j.1365-313X.2003.01811.x","article-title":"Absence of the Lhcb1 and Lhcb2 proteins of the light-harvesting complex of photosystem II\u2014Effects on photosynthesis, grana stacking and fitness","volume":"35","author":"Andersson","year":"2003","journal-title":"Plant J."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"977","DOI":"10.1038\/415977a","article-title":"MAP kinase signalling cascade in Arabidopsis innate immunity","volume":"415","author":"Asai","year":"2002","journal-title":"Nature"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"418","DOI":"10.1038\/nature08794","article-title":"Differential innate immune signalling via Ca2+ sensor protein kinases","volume":"464","author":"Boudsocq","year":"2010","journal-title":"Nature"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"1583","DOI":"10.2307\/3869945","article-title":"Characterization of an Arabidopsis mutant that is nonresponsive to inducers of systemic acquired resistance","volume":"6","author":"Cao","year":"1994","journal-title":"Plant Cell"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1080\/07352689.2011.616043","article-title":"Plant chitinases: Genetic diversity and physiological roles","volume":"31","author":"Grover","year":"2012","journal-title":"Crit. Rev. Plant Sci."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1016\/S0014-5793(98)01251-4","article-title":"Wounding and chemicals induce expression of the Arabidopsis thaliana gene Thi2.1, encoding a fungal defense thionin, via the octadecanoid pathway","volume":"437","author":"Bohlmann","year":"1998","journal-title":"FEBS Lett."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"370","DOI":"10.1021\/acs.chemrestox.9b00028","article-title":"RBOH-dependent ROS synthesis and ROS scavenging by plant specialized metabolites to modulate plant development and stress responses","volume":"32","author":"Chapman","year":"2019","journal-title":"Chem. Res. Toxicol."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"442","DOI":"10.1038\/nature01485","article-title":"Reactive oxygen species produced by NADPH oxidase regulate plant cell growth","volume":"422","author":"Foreman","year":"2003","journal-title":"Nature"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1071\/FP18219","article-title":"Hydrogen peroxide plays an important role in PERK4-mediated abscisic acid-regulated root growth in Arabidopsis","volume":"46","author":"Ma","year":"2019","journal-title":"Funct. Plant Biol."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1111\/j.1574-6941.2010.00860.x","article-title":"Are root exudates more important than other sources of rhizodeposits in structuring rhizosphere bacterial communities?","volume":"72","author":"Dennis","year":"2010","journal-title":"FEMS Microbiol. Ecol."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"2038","DOI":"10.1021\/acs.jnatprod.9b00110","article-title":"Bioactive secondary metabolites from Bacillus subtilis: A comprehensive review","volume":"82","author":"Kaspar","year":"2019","journal-title":"J. Nat. Prod."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"869","DOI":"10.1007\/s10886-013-0319-7","article-title":"Ecological and mechanistic insights into the direct and indirect antimicrobial properties of Bacillus subtilis lipopeptides on plant pathogens","volume":"39","author":"Falardeau","year":"2013","journal-title":"J. Chem. Ecol."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"dev185819","DOI":"10.1242\/dev.185819","article-title":"Flavonols regulate root hair development by modulating accumulation of reactive oxygen species in the root epidermis","volume":"147","author":"Gayomba","year":"2020","journal-title":"Development"},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"36","DOI":"10.3389\/fpls.2017.00036","article-title":"Arabidopsis NITRILASE 1 contributes to the regulation of root growth and development through modulation of auxin biosynthesis in seedlings","volume":"8","author":"Lehmann","year":"2017","journal-title":"Front. Plant Sci."},{"key":"ref_74","first-page":"1127500","article-title":"Portraying mechanics of plant growth promoting rhizobacteria (PGPR): A review","volume":"2","author":"Goswami","year":"2016","journal-title":"Cogent Food Agric."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"1007","DOI":"10.1007\/s10886-013-0317-9","article-title":"Dynamic chemical communication between plants and bacteria through airborne signals: Induced resistance by bacterial volatiles","volume":"39","author":"Farag","year":"2013","journal-title":"J. Chem. Ecol."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1007\/s00203-022-03216-x","article-title":"The antifungal activity of a serine protease and the enzyme production of characteristics of Bacillus licheniformis TG116","volume":"204","author":"Ling","year":"2022","journal-title":"Arch. Microbiol."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1038\/nature14243","article-title":"Pathogen-secreted proteases activate a novel plant immune pathway","volume":"521","author":"Cheng","year":"2015","journal-title":"Nature"},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1016\/j.ejsobi.2010.11.001","article-title":"The siderophore-producing bacterium, Bacillus subtilis CAS15, has a biocontrol effect on Fusarium wilt and promotes the growth of pepper","volume":"47","author":"Yu","year":"2011","journal-title":"Eur. J. Soil. Biol."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"ovac026","DOI":"10.1093\/lambio\/ovac026","article-title":"Bacillibactin class siderophores produced by the endophyte Bacillus subtilis NPROOT3 as antimycobacterial agents","volume":"76","author":"Nalli","year":"2023","journal-title":"Lett. Appl. Microbiol."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/S0168-1656(03)00022-1","article-title":"Extracellular polysaccharide synthesis by Nostoc strains as affected by N source and light intensity","volume":"102","author":"Otero","year":"2003","journal-title":"J. Biotechnol."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1186\/1475-2859-5-7","article-title":"Rhizobial exopolysaccharides: Genetic control and symbiotic functions","volume":"5","author":"Skorupska","year":"2006","journal-title":"Microb. Cell Factories"},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"689","DOI":"10.1080\/17429145.2014.902125","article-title":"Role of plant growth-promoting rhizobacteria and their exopolysaccharide in drought tolerance of maize","volume":"9","author":"Naseem","year":"2014","journal-title":"J. Plant Interact."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"1009","DOI":"10.1002\/jobm.201800309","article-title":"Exopolysaccharides producing rhizobacteria and their role in plant growth and drought tolerance","volume":"58","author":"Naseem","year":"2018","journal-title":"J. Basic Microbiol."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"1961","DOI":"10.18520\/cs\/v111\/i12\/1961-1967","article-title":"Amelioration of soybean plant from saline-induced condition by exopolysaccharide producing Pseudomonas-mediated expression of high affinity K+-transporter (HKT1) gene","volume":"111","author":"Kasotia","year":"2016","journal-title":"Curr. Sci."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"943","DOI":"10.1111\/nph.14491","article-title":"Starch as a determinant of plant fitness under abiotic stress","volume":"214","author":"Thalmann","year":"2017","journal-title":"New Phytol."},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"1593","DOI":"10.1093\/jxb\/err460","article-title":"Drought, salt, and temperature stress-induced metabolic rearrangements and regulatory networks","volume":"63","author":"Krasensky","year":"2012","journal-title":"J. Exp. Bot."},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"1674","DOI":"10.1104\/pp.104.040808","article-title":"\u03b2-Amylase induction and the protective role of maltose during temperature shock","volume":"135","author":"Kaplan","year":"2004","journal-title":"Plant Physiol."},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1104\/pp.109.148965","article-title":"Developmental stage specificity and the role of mitochondrial metabolism in the response of Arabidopsis leaves to prolonged mild osmotic stress","volume":"152","author":"Skirycz","year":"2010","journal-title":"Plant Physiol."},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"602","DOI":"10.1111\/pce.12836","article-title":"Subcellular reprogramming of metabolism during cold acclimation in Arabidopsis thaliana","volume":"40","author":"Hoermiller","year":"2017","journal-title":"Plant Cell Environ."},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"644","DOI":"10.1111\/ppl.12759","article-title":"Abscisic acid prevents pollen abortion under high-temperature stress by mediating sugar metabolism in rice spikelets","volume":"165","author":"Rezaul","year":"2019","journal-title":"Physiol. Plant"},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"5979","DOI":"10.1093\/jxb\/ers246","article-title":"Binding of ABI4 to a CACCG motif mediates the ABA-induced expression of the ZmSSI gene in maize (Zea mays L.) endosperm","volume":"63","author":"Hu","year":"2012","journal-title":"J. Exp. Bot."},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"838","DOI":"10.1104\/pp.103.024513","article-title":"ADP-glucose pyrophosphorylase is activated by posttranslational redox-modification in response to light and to sugars in leaves of Arabidopsis and other plant species","volume":"133","author":"Hendriks","year":"2003","journal-title":"Plant Physiol."},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1046\/j.1365-313X.2001.2641043.x","article-title":"Impaired sucrose-induction mutants reveal the modulation of sugar-induced starch biosynthetic gene expression by abscisic acid signalling","volume":"26","author":"Rook","year":"2001","journal-title":"Plant J."},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jplph.2019.01.004","article-title":"ABA promotes starch synthesis and storage metabolism in dormant grapevine buds","volume":"234\u2013235","author":"Rubio","year":"2019","journal-title":"J. Plant Physiol."},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1111\/j.1365-313X.2007.03318.x","article-title":"Duplicated P5CS genes of Arabidopsis play distinct roles in stress regulation and developmental control of proline biosynthesis","volume":"53","author":"Zsigmond","year":"2008","journal-title":"Plant J."},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1046\/j.1365-313X.1997.00537.x","article-title":"Differential expression of two P5CS genes controlling proline accumulation during salt-stress requires ABA and is regulated by ABA1, ABI1 and AXR2 in Arabidopsis","volume":"12","author":"Strizhov","year":"1997","journal-title":"Plant J."},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/S0022-2836(05)80360-2","article-title":"Basic local alignment search tool","volume":"215","author":"Altschul","year":"1990","journal-title":"J. Mol. Biol."},{"key":"ref_98","doi-asserted-by":"crossref","first-page":"1547","DOI":"10.1093\/molbev\/msy096","article-title":"MEGA X: Molecular evolutionary genetics analysis across computing platforms","volume":"35","author":"Kumar","year":"2018","journal-title":"Mol. Biol. Evol."},{"key":"ref_99","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1016\/j.mimet.2008.07.004","article-title":"Evaluation of isolation methods and RNA integrity for bacterial RNA quantitation","volume":"7","author":"Jahn","year":"2008","journal-title":"J. Microbiol. Methods"},{"key":"ref_100","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1089\/cmb.2012.0021","article-title":"SPAdes: A new genome assembly algorithm and its applications to single-cell sequencing","volume":"19","author":"Bankevich","year":"2012","journal-title":"J. Comput. Biol."},{"key":"ref_101","doi-asserted-by":"crossref","first-page":"3100","DOI":"10.1093\/nar\/gkm160","article-title":"RNammer: Consistent annotation of rRNA genes in genomic sequences","volume":"35","author":"Lagesen","year":"2007","journal-title":"Nucleic Acids Res."},{"key":"ref_102","first-page":"1","article-title":"tRNAscan-SE: Searching for tRNA genes in genomic sequences","volume":"Volume 1962","author":"Chan","year":"2019","journal-title":"Gene Prediction"},{"key":"ref_103","doi-asserted-by":"crossref","first-page":"4636","DOI":"10.1093\/nar\/27.23.4636","article-title":"Improved microbial gene identification with GLIMMER","volume":"27","author":"Delcher","year":"1999","journal-title":"Nucleic Acids Res."},{"key":"ref_104","doi-asserted-by":"crossref","unstructured":"Tatusov, R.L., Fedorova, N.D., Jackson, J.D., Jacobs, A.R., Kiryutin, B., Koonin, E.V., Krylov, D.M., Mazumder, R., Mekhedov, S.L., and Nikolskaya, A.N. (2003). The COG database: An updated version includes eukaryotes. BMC Bioinform., 4.","DOI":"10.1186\/1471-2105-4-41"},{"key":"ref_105","doi-asserted-by":"crossref","first-page":"W182","DOI":"10.1093\/nar\/gkm321","article-title":"KAAS: An automatic genome annotation and pathway reconstruction server","volume":"35","author":"Moriya","year":"2007","journal-title":"Nucleic Acids Res."},{"key":"ref_106","doi-asserted-by":"crossref","unstructured":"Chen, T.W., Gan, R.C.R., Wu, T.H., Huang, P.J., Lee, C.Y., Chen, Y.Y.M., Chen, C.C., and Tang, P. (2012). FastAnnotator: An efficient transcript annotation web tool. BMC Genom., 13.","DOI":"10.1186\/1471-2164-13-S7-S9"},{"key":"ref_107","first-page":"D517","article-title":"CARD 2020: Antibiotic resistome surveillance with the comprehensive antibiotic resistance database","volume":"48","author":"Alcock","year":"2020","journal-title":"Nucleic Acids Res."},{"key":"ref_108","doi-asserted-by":"crossref","first-page":"1","DOI":"10.9734\/mrji\/2019\/v29i530174","article-title":"Isolation, screening and characterization of exopolysaccharide producing bacteria","volume":"29","author":"Nwosu","year":"2019","journal-title":"Microbiol. Res. J. Int."},{"key":"ref_109","doi-asserted-by":"crossref","first-page":"1395","DOI":"10.3389\/fpls.2017.01395","article-title":"The phenylpropanoid pathway and lignin in defense against Ganoderma boninense colonized root tissues in oil palm (Elaeis guineensis Jacq.)","volume":"8","author":"Govender","year":"2017","journal-title":"Front. Plant Sci."},{"key":"ref_110","doi-asserted-by":"crossref","first-page":"889","DOI":"10.1104\/pp.91.3.889","article-title":"Elicitation of lignin biosynthesis and isoperoxidase activity by pectic fragments in suspension cultures of castor bean","volume":"91","author":"Bruce","year":"1989","journal-title":"Plant Physiol."},{"key":"ref_111","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1021\/jf0106695","article-title":"Using a modified ferrous oxidation\u2212xylenol orange (FOX) assay for detection of lipid hydroperoxides in plant tissue","volume":"50","author":"DeLong","year":"2002","journal-title":"J. Agric. Food Chem."},{"key":"ref_112","first-page":"867","article-title":"Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts","volume":"22","author":"Nakano","year":"1981","journal-title":"Plant Cell Physiol."},{"key":"ref_113","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/S0076-6879(84)05016-3","article-title":"Catalase in vitro","volume":"105","author":"Aebi","year":"1984","journal-title":"Methods Enzymol."},{"key":"ref_114","doi-asserted-by":"crossref","first-page":"1582","DOI":"10.1104\/pp.109.144196","article-title":"Starch synthesis in Arabidopsis is achieved by spatial cotranscription of core starch metabolism genes","volume":"151","author":"Tsai","year":"2009","journal-title":"Plant Physiol."},{"key":"ref_115","doi-asserted-by":"crossref","first-page":"1260","DOI":"10.1111\/jam.15252","article-title":"Rhizobacterial Bacillus mycoides functions in stimulating the antioxidant defence system and multiple phytohormone signalling pathways to regulate plant growth and stress tolerance","volume":"132","author":"Kurniawan","year":"2022","journal-title":"J. Appl. Microbiol."},{"key":"ref_116","doi-asserted-by":"crossref","first-page":"685","DOI":"10.1111\/j.1365-313X.1992.tb00137.x","article-title":"A Brassica napus transcript encoding a protein related to the K\u00fcnitz protease inhibitor family accumulates upon water stress in leaves, not in seeds","volume":"2","author":"Mauxion","year":"1992","journal-title":"Plant J."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/18\/13720\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T20:45:54Z","timestamp":1760129154000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/18\/13720"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,9,6]]},"references-count":116,"journal-issue":{"issue":"18","published-online":{"date-parts":[[2023,9]]}},"alternative-id":["ijms241813720"],"URL":"https:\/\/doi.org\/10.3390\/ijms241813720","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,9,6]]}}}