{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T15:47:50Z","timestamp":1787068070431,"version":"build-2736575974"},"reference-count":69,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2023,9,6]],"date-time":"2023-09-06T00:00:00Z","timestamp":1693958400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Fukui University of Technology","award":["FY 2023"],"award-info":[{"award-number":["FY 2023"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Salt stress of soybean is a serious problem because it reduces plant growth and seed yield. To investigate the salt-tolerant mechanism of soybean, a plant-derived smoke (PDS) solution was used. Three-day-old soybeans were subjected to PDS solution under 100 mM NaCl for 2 days, resulting in PDS solution improving soybean root growth, even under salt stress. Under the same condition, proteins were analyzed using the proteomic technique. Differential abundance proteins were associated with transport\/formaldehyde catabolic process\/sucrose metabolism\/glutathione metabolism\/cell wall organization in the biological process and membrane\/Golgi in the cellular component with or without PDS solution under salt stress. Immuno-blot analysis confirmed that osmotin, alcohol dehydrogenase, and sucrose synthase increased with salt stress and decreased with additional PDS solution; however, H+ATPase showed opposite effects. Cellulose synthase and xyloglucan endotransglucosylase\/hydrolase increased with salt and decreased with additional PDS solution. Furthermore, glycoproteins decreased with salt stress and recovered with additional treatment. As mitochondrion-related events, the contents of ATP and gamma-aminobutyric acid increased with salt stress and recovered with additional treatment. These results suggest that PDS solution improves the soybean growth by alleviating salt stress. Additionally, the regulation of energy metabolism, protein glycosylation, and cell wall construction might be an important factor for the acquisition of salt tolerance in soybean.<\/jats:p>","DOI":"10.3390\/ijms241813734","type":"journal-article","created":{"date-parts":[[2023,9,6]],"date-time":"2023-09-06T10:07:37Z","timestamp":1693994857000},"page":"13734","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Proteomic Analysis Reveals Salt-Tolerant Mechanism in Soybean Applied with Plant-Derived Smoke Solution"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4514-357X","authenticated-orcid":false,"given":"Setsuko","family":"Komatsu","sequence":"first","affiliation":[{"name":"Faculty of Environment and Information Sciences, Fukui University of Technology, Fukui 910-8505, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taiki","family":"Kimura","sequence":"additional","affiliation":[{"name":"Faculty of Environment and Information Sciences, Fukui University of Technology, Fukui 910-8505, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7955-182X","authenticated-orcid":false,"given":"Shafiq Ur","family":"Rehman","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Haripur, Haripur 22620, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4507-9598","authenticated-orcid":false,"given":"Hisateru","family":"Yamaguchi","sequence":"additional","affiliation":[{"name":"Department of Medical Technology, Yokkaichi Nursing and Medical Care University, Yokkaichi 512-8045, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keisuke","family":"Hitachi","sequence":"additional","affiliation":[{"name":"Institute for Comprehensive Medical Science, Fujita Health University, Toyoake 470-1192, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kunihiro","family":"Tsuchida","sequence":"additional","affiliation":[{"name":"Institute for Comprehensive Medical Science, Fujita Health University, Toyoake 470-1192, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,9,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Bhoite, R., Han, Y., Chaitanya, A.K., Varshney, R.K., and Sharma, D.L. (2023). Genomic approaches to enhance adaptive plasticity to cope with soil constraints amidst climate change in wheat. Plant Genome, e20358.","DOI":"10.1002\/tpg2.20358"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"e0136621","DOI":"10.1128\/AEM.01366-21","article-title":"High salinity inhibits soil bacterial community mediating nitrogen cycling","volume":"87","author":"Li","year":"2021","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Sarri, E., Termentzi, A., Abraham, E.M., Papadopoulos, G.K., Baira, K., Machera, K., Loukas, V., Komaitis, F., and Tani, E. (2021). Salinity stress alters the secondary metabolic profile of M. sativa, M. arborea and their hybrid (Alborea). Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22094882"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"111383","DOI":"10.1016\/j.jenvman.2020.111383","article-title":"Soil salinization management for sustainable development: A review","volume":"277","author":"Singh","year":"2021","journal-title":"J. Environ. Manag."},{"key":"ref_5","unstructured":"Meena, R.S., and Datta, R. (2020). Soil Moisture Importance, IntechOpen."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1194","DOI":"10.3389\/fpls.2020.01194","article-title":"Commentary: Bread wheat with high salinity and sodicity tolerance","volume":"11","author":"McDonald","year":"2020","journal-title":"Front. Plant Sci."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1196","DOI":"10.1111\/j.1744-7909.2008.00760.x","article-title":"Salt tolerance in soybean","volume":"50","author":"Phang","year":"2008","journal-title":"J. Integr. Plant Biol."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Chen, R., Li, M., Zhang, H., Duan, L., Sun, X., Jiang, Q., Zhang, H., and Hu, Z. (2019). Continuous salt stress-induced long non-coding RNAs and DNA methylation patterns in soybean roots. BMC Genomics, 20.","DOI":"10.1186\/s12864-019-6101-7"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"644408","DOI":"10.3389\/fpls.2021.644408","article-title":"Galactolipid and phospholipid profile and proteome alterations in soybean leaves at the onset of salt stress","volume":"12","author":"Liu","year":"2021","journal-title":"Front. Plant Sci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1142780","DOI":"10.3389\/fmicb.2023.1142780","article-title":"Salt altered rhizosphere fungal community and induced soybean recruit specific species to ameliorate salt stress","volume":"14","author":"Yuan","year":"2023","journal-title":"Front. Microbiol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1787","DOI":"10.3389\/fpls.2016.01787","article-title":"New insights on plant salt tolerance mechanisms and their potential use for breeding","volume":"7","author":"Hanin","year":"2016","journal-title":"Front. Plant Sci."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"2027","DOI":"10.1002\/jsfa.10822","article-title":"Seed priming and foliar application with jasmonic acid enhance salinity stress tolerance of soybean (Glycine max L.) seedlings","volume":"101","author":"Sheteiwy","year":"2021","journal-title":"J. Sci. Food Agric."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1126\/science.abb0355","article-title":"Fire and biodiversity in the Anthropocene","volume":"370","author":"Kelly","year":"2020","journal-title":"Science"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"155","DOI":"10.17660\/ActaHortic.2009.813.20","article-title":"Karrikinolide\u2014A phytoreactive compound derived from smoke with applications in horticulture, ecological restoration and agriculture","volume":"813","author":"Dixon","year":"2009","journal-title":"Acta Hortic."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Khatoon, A., Rehman, S.U., Aslam, M.M., Jamil, M., and Komatsu, S. (2020). Plant-derived smoke affects biochemical mechanism on plant growth and seed germination. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21207760"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Bose, U., Juh\u00e1sz, A., Broadbent, J.A., Komatsu, S., and Colgrave, M.L. (2020). Multi-omics strategies for decoding smoke-assisted germina- tion pathways and seed vigour. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21207512"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"17119","DOI":"10.1038\/nplants.2017.119","article-title":"Acetate-mediated novel survival strategy against drought in plants","volume":"3","author":"Kim","year":"2017","journal-title":"Nat. Plants"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1111\/ppl.12836","article-title":"The karrikin \u201ccalisthenics\u201d: Can compounds derived from smoke help in stress tolerance?","volume":"165","author":"Banerjee","year":"2019","journal-title":"Physiol. Plant."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"2189","DOI":"10.1093\/jxb\/erx476","article-title":"From carotenoids to strigolactones","volume":"69","author":"Jia","year":"2018","journal-title":"J. Exp. Bot."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Yang, T., Lian, Y., and Wang, C. (2019). Comparing and Contrasting the Multiple Roles of Butenolide Plant Growth Regulators: Strigolactones and Karrikins in Plant Development and Adaptation to Abiotic Stresses. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20246270"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"23850","DOI":"10.1007\/s11356-017-0026-y","article-title":"Bacillus safensis with plant-derived smoke stimulates rice growth under saline conditions","volume":"24","author":"Khan","year":"2017","journal-title":"Environ. Sci. Pollut. Res. Int."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1007\/s11105-020-01230-1","article-title":"Proteomic study to understand promotive effects of plant-derived smoke on soybean root growth under flooding stress","volume":"39","author":"Otori","year":"2021","journal-title":"Plant Mol. Biol. Rep."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Murashita, Y., Nishiuchi, T., Rehman, S.U., and Komatsu, S. (2021). Subcellular proteomics to understand promotive effect of plant-derived smoke solution on soybean root. Proteomes, 9.","DOI":"10.3390\/proteomes9040039"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"103781","DOI":"10.1016\/j.jprot.2020.103781","article-title":"Plant-derived smoke enhances plant growth through ornithine-synthesis pathway and ubiquitin-proteasome pathway in soybean","volume":"221","author":"Zhong","year":"2020","journal-title":"J. Proteomics"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1016\/j.jprot.2018.04.031","article-title":"Proteomic analysis of the effect of plant-derived smoke on soybean during recovery from flooding stress","volume":"181","author":"Li","year":"2018","journal-title":"J. Proteomics"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Aslam, M.M., Rehman, S., Khatoon, A., Jamil, M., Yamaguchi, H., Hitachi, K., Tsuchida, K., Li, X., Sunohara, Y., and Matsumoto, H. (2019). Molecular responses of maize shoot to a plant derived smoke solution. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20061319"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.jprot.2018.01.011","article-title":"Proteomic analysis of the promotive effect of plant-derived smoke on plant growth of chickpea","volume":"176","author":"Rehman","year":"2018","journal-title":"J. Proteom."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"850","DOI":"10.1038\/nrm1746","article-title":"Growth of the plant cell wall","volume":"6","author":"Cosgrove","year":"2005","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1277","DOI":"10.1016\/j.ijbiomac.2019.11.099","article-title":"Isolation and characterization of Populus xyloglucan endotransglycosylase\/hydrolase (XTH) involved in osmotic stress responses","volume":"155","author":"Jiang","year":"2020","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1467","DOI":"10.1093\/aob\/mcm248","article-title":"Xyloglucan endotransglucosylase activity loosens a plant cell wall","volume":"100","author":"Suslov","year":"2007","journal-title":"Ann. Bot."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"4731","DOI":"10.1105\/tpc.112.106039","article-title":"XTH31, Encoding an in vitro XEH\/XET-active enzyme, regulates aluminum sensitivity by modulating in vivo XET action, cell wall xyloglucan content, and aluminum binding capacity in Arabidopsis","volume":"24","author":"Zhu","year":"2012","journal-title":"Plant Cell."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/j.ecoenv.2016.06.007","article-title":"Identification of a group of XTHs genes responding to heavy metal mercury, salinity and drought stresses in Medicago truncatula","volume":"132","author":"Xuan","year":"2016","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1093\/aob\/mcw062","article-title":"Spatial\u2013temporal analysis of polyethylene glycol-reduced aluminium accumulation and xyloglucan endotransglucosylase action in root tips of common bean (Phaseolus vulgaris)","volume":"118","author":"Zhang","year":"2016","journal-title":"Ann. Bot."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"867","DOI":"10.1007\/s00299-010-0989-3","article-title":"Constitutive expression of CaXTH3, a hot pepper xyloglucan endotransglucosylase\/hydrolase, enhanced tolerance to salt and drought stresses without phenotypic defects in tomato plants (Solanum lycopersicum cv. Dotaerang)","volume":"30","author":"Choi","year":"2011","journal-title":"Plant Cell Rep."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Song, L., Valliyodan, B., Prince, S., Wan, J., and Nguyen, H.T. (2018). Characterization of the XTH gene family: New insight to the roles in soybean flooding tolerance. Int. J. Mol. Sci., 19.","DOI":"10.3390\/ijms19092705"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1491","DOI":"10.1126\/science.1126551","article-title":"Visualization of cellulose synthase demonstrates functional association with microtubules","volume":"312","author":"Paredez","year":"2006","journal-title":"Science"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"12637","DOI":"10.1073\/pnas.93.22.12637","article-title":"Higher plants contain homologs of the bacterial celA genes encoding the catalytic subunit of cellulose synthase","volume":"93","author":"Pear","year":"1996","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1521","DOI":"10.1104\/pp.114.241216","article-title":"The cellulase KORRIGAN is part of the cellulose synthase complex","volume":"165","author":"Vain","year":"2014","journal-title":"Plant Physiol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"12866","DOI":"10.1073\/pnas.1007092107","article-title":"Identification of a cellulose synthase-associated protein required for cellulose biosynthesis","volume":"107","author":"Gu","year":"2010","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1353","DOI":"10.1016\/j.cell.2015.08.028","article-title":"A mechanism for sustained cellulose synthesis during salt stress","volume":"162","author":"Endler","year":"2015","journal-title":"Cell"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"857","DOI":"10.1038\/s41467-019-08780-3","article-title":"The companion of cellulose synthase 1 confers salt tolerance through a tau-like mechanism in plants","volume":"10","author":"Kesten","year":"2019","journal-title":"Nat. Comm."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"41397","DOI":"10.1038\/srep41397","article-title":"Knockdown of a cellulose synthase gene BoiCesA affects the leaf anatomy, cellulose content and salt tolerance in broccoli","volume":"7","author":"Li","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"448","DOI":"10.1038\/nrm3383","article-title":"Vertebrate protein glycosylation: Diversity, synthesis and function","volume":"13","author":"Moremen","year":"2012","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"47692","DOI":"10.1074\/jbc.M208136200","article-title":"Studies on the function of oligosaccharyl transferase subunits. Stt3p is directly involved in the glycosylation process","volume":"277","author":"Yan","year":"2002","journal-title":"J. Biol. Chem."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"47R","DOI":"10.1093\/glycob\/cwj066","article-title":"An evolving view of the eukaryotic oligosaccharyltransferase","volume":"16","author":"Kelleher","year":"2006","journal-title":"Glycobiology"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1016\/j.plantsci.2018.05.007","article-title":"Function of N-glycosylation in plants","volume":"274","author":"Nagashima","year":"2018","journal-title":"Plant Sci."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1093\/glycob\/cwp170","article-title":"Two Arabidopsis thaliana Golgi alpha-mannosidase I enzymes are responsible for plant N-glycan maturation","volume":"20","author":"Kajiura","year":"2010","journal-title":"Glycobiology"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"787","DOI":"10.1023\/A:1007127815012","article-title":"Molecular cloning of cDNA encoding N-acetylglucosaminyltransferase II from Arabidopsis thaliana","volume":"16","author":"Strasser","year":"1999","journal-title":"Glycoconj. J."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"678","DOI":"10.1016\/j.molp.2018.01.006","article-title":"Trimming of N-glycans by the Golgi-Localized \u03b1-1,2-Mannosidases, MNS1 and MNS2, is crucial for maintaining RSW2 protein abundance during salt stress in Arabidopsis","volume":"11","author":"Liu","year":"2018","journal-title":"Mol. Plant."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"646425","DOI":"10.3389\/fpls.2021.646425","article-title":"Comparative label-free quantitative proteomics analysis reveals the essential roles of N-glycans in salt tolerance by modulating protein abundance in Arabidopsis","volume":"12","author":"Liu","year":"2021","journal-title":"Front. Plant Sci."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1007\/s11306-018-1440-y","article-title":"NMR-based metabolomics reveals that plant-derived smoke stimulates root growth via affecting carbohydrate and energy metabolism in maize","volume":"14","author":"Elgin","year":"2018","journal-title":"Metabolomics"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1146\/annurev.arplant.59.032607.092911","article-title":"Mechanisms of salinity tolerance","volume":"64","author":"Munns","year":"2008","journal-title":"Ann. Rev. Plant Biol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"4807","DOI":"10.1021\/pr400504a","article-title":"Investigating the role of respiration in plant salinity tolerance by analyzing mitochondrial proteomes from wheat and a salinity-tolerant Amphiploid (wheat \u00d7 Lophopyrum elongatum)","volume":"12","author":"Jacoby","year":"2013","journal-title":"J Proteome Res."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"100","DOI":"10.3389\/fpls.2018.00100","article-title":"GABA shunt in durum wheat","volume":"9","author":"Carillo","year":"2018","journal-title":"Front. Plant Sci."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1166","DOI":"10.1111\/nph.15713","article-title":"Wheat mitochondrial respiration shifts from the tricarboxylic acid cycle to the GABA shunt under salt stress","volume":"225","author":"Jacoby","year":"2020","journal-title":"New Phytol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"95","DOI":"10.3389\/fpls.2019.00095","article-title":"An overview of sucrose synthases in plants","volume":"10","author":"Stein","year":"2019","journal-title":"Front. Plant Sci."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"17905","DOI":"10.1039\/C8RA02996B","article-title":"iTRAQ-based quantitative proteomic analysis of dark-germinated soybeans in response to salt stress","volume":"8","author":"Yin","year":"2018","journal-title":"RSC Adv."},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Al-Quraan, N.A., Samarah, N.H., and Tanash, A.A. (2022). Effect of drought stress on wheat (Triticum durum) growth and metabolism: Insight from GABA shunt, reactive oxygen species and dehydrin genes expression. Funct. Plant Biol.","DOI":"10.1071\/FP22177"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1071\/FP22211","article-title":"Ultrasonic treatment to enhance seed germination and vigour of wheat (Triticum durum) in association with \u03b3-aminobutyric acid (GABA) shunt pathway","volume":"50","author":"Samarah","year":"2023","journal-title":"Funct. Plant Biol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1071\/BT96119","article-title":"Germination of four species of native Western Australian plants using plant-derived smoke","volume":"47","author":"Tieu","year":"1999","journal-title":"Aust. J. Bot."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding","volume":"72","author":"Bradford","year":"1976","journal-title":"Anal. Biochem."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"4769","DOI":"10.1021\/pr4001898","article-title":"Label-free quantitative proteomic analysis of abscisic acid effect in early-stage soybean under flooding","volume":"12","author":"Komatsu","year":"2013","journal-title":"J. Proteome Res."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"731","DOI":"10.1038\/nmeth.3901","article-title":"The Perseus computational platform for comprehensive analysis of (prote) omics data","volume":"13","author":"Tyanova","year":"2016","journal-title":"Nat. Methods"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"1162","DOI":"10.1002\/1615-9861(200109)1:9<1162::AID-PROT1162>3.0.CO;2-S","article-title":"A proteomics approach towards understanding blast fungus infection of rice grown under different levels of nitrogen fertilization","volume":"1","author":"Konishi","year":"2001","journal-title":"Proteomics"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1007\/s11103-011-9812-y","article-title":"Characterization of a novel flooding stress-responsive alcohol dehydrogenase expressed in soybean roots","volume":"77","author":"Komatsu","year":"2011","journal-title":"Plant Mol. Biol."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"3989","DOI":"10.1021\/pr100179f","article-title":"Comparative proteomic analysis of early-stage soybean seedlings responses to flooding by using gel and gel-free techniques","volume":"9","author":"Nanjo","year":"2010","journal-title":"J. Proteome Res."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1080\/09168451.2019.1661768","article-title":"GABA enzymatic assay kit","volume":"84","author":"Nishiyama","year":"2020","journal-title":"Biosci. Biotech. Biochem."},{"key":"ref_68","first-page":"D1063","article-title":"PRoteomics IDEntifications (PRIDE) database and associated tools: Status in 2013","volume":"41","author":"Csordas","year":"2013","journal-title":"Nucleic Acids Res."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"D1107","DOI":"10.1093\/nar\/gkw1080","article-title":"jPOSTTrepo: An international standard data repository for proteomes","volume":"45","author":"Okuda","year":"2017","journal-title":"Nucleic Acids Res."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/18\/13734\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T20:45:58Z","timestamp":1760129158000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/18\/13734"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,9,6]]},"references-count":69,"journal-issue":{"issue":"18","published-online":{"date-parts":[[2023,9]]}},"alternative-id":["ijms241813734"],"URL":"https:\/\/doi.org\/10.3390\/ijms241813734","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,9,6]]}}}