{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T04:52:57Z","timestamp":1785214377172,"version":"3.55.0"},"reference-count":70,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2023,9,9]],"date-time":"2023-09-09T00:00:00Z","timestamp":1694217600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Realizzazione di Servizi di Ricerca e Sviluppo per la Lotta contro il COVID-19\u201d","award":["CUP B63C22001230002"],"award-info":[{"award-number":["CUP B63C22001230002"]}]},{"name":"Realizzazione di Servizi di Ricerca e Sviluppo per la Lotta contro il COVID-19\u201d","award":["2017M8R7N9"],"award-info":[{"award-number":["2017M8R7N9"]}]},{"name":"Realizzazione di Servizi di Ricerca e Sviluppo per la Lotta contro il COVID-19\u201d","award":["PE00000007, INF-ACT"],"award-info":[{"award-number":["PE00000007, INF-ACT"]}]},{"name":"Natural and pharmacological inhibition of the early phase of viral replication (Vir-SudNet)","award":["CUP B63C22001230002"],"award-info":[{"award-number":["CUP B63C22001230002"]}]},{"name":"Natural and pharmacological inhibition of the early phase of viral replication (Vir-SudNet)","award":["2017M8R7N9"],"award-info":[{"award-number":["2017M8R7N9"]}]},{"name":"Natural and pharmacological inhibition of the early phase of viral replication (Vir-SudNet)","award":["PE00000007, INF-ACT"],"award-info":[{"award-number":["PE00000007, INF-ACT"]}]},{"name":"NextGeneration EU-MUR PNRR Extended Partnership initiative on Emerging Infectious Diseases","award":["CUP B63C22001230002"],"award-info":[{"award-number":["CUP B63C22001230002"]}]},{"name":"NextGeneration EU-MUR PNRR Extended Partnership initiative on Emerging Infectious Diseases","award":["2017M8R7N9"],"award-info":[{"award-number":["2017M8R7N9"]}]},{"name":"NextGeneration EU-MUR PNRR Extended Partnership initiative on Emerging Infectious Diseases","award":["PE00000007, INF-ACT"],"award-info":[{"award-number":["PE00000007, INF-ACT"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Pandemic and epidemic outbreaks of respiratory viruses are a challenge for public health and social care system worldwide, leading to high mortality and morbidity among the human populations. In light of the limited efficacy of current vaccines and antiviral drugs against respiratory viral infections and the emergence and re-emergence of new viruses, novel broad-spectrum antiviral drugs are needed for the prevention and treatment of these infections. Antimicrobial peptides with an antiviral effect, also known as AVPs, have already been reported as potent inhibitors of viral infections by affecting different stages of the virus lifecycle. In the present study, we analyzed the activity of the AVP Hylin-a1, secreted by the frog Hypsiboas albopunctatus, against a wide range of respiratory viruses, including the coronaviruses HCoV-229E and SARS-CoV-2, measles virus, human parainfluenza virus type 3, and influenza virus H1N1. We report a significant inhibitory effect on infectivity in all the enveloped viruses, whereas there was a lack of activity against the naked coxsackievirus B3. Considering the enormous therapeutic potential of Hylin-a1, further experiments are required to elucidate its mechanism of action and to increase its stability by modifying the native sequence.<\/jats:p>","DOI":"10.3390\/ijms241813888","type":"journal-article","created":{"date-parts":[[2023,9,11]],"date-time":"2023-09-11T09:00:52Z","timestamp":1694422852000},"page":"13888","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Hylin-a1: A Pan-Inhibitor against Emerging and Re-Emerging Respiratory Viruses"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1648-4519","authenticated-orcid":false,"given":"Annalisa","family":"Chianese","sequence":"first","affiliation":[{"name":"Department of Experimental Medicine, University of Campania \u201cLuigi Vanvitelli\u201d, 80138 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7991-8700","authenticated-orcid":false,"given":"Carla","family":"Zannella","sequence":"additional","affiliation":[{"name":"Department of Experimental Medicine, University of Campania \u201cLuigi Vanvitelli\u201d, 80138 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandra","family":"Monti","sequence":"additional","affiliation":[{"name":"Institute of Biostructures and Bioimaging (IBB), National Research Council (CNR), 80131 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2952-6658","authenticated-orcid":false,"given":"Nunzianna","family":"Doti","sequence":"additional","affiliation":[{"name":"Institute of Biostructures and Bioimaging (IBB), National Research Council (CNR), 80131 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3999-4263","authenticated-orcid":false,"given":"Giuseppina","family":"Sanna","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, University of Cagliari, Cittadella Universitaria, 09042 Monserrato, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2961-3428","authenticated-orcid":false,"given":"Aldo","family":"Manzin","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, University of Cagliari, Cittadella Universitaria, 09042 Monserrato, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0395-7962","authenticated-orcid":false,"given":"Anna","family":"De Filippis","sequence":"additional","affiliation":[{"name":"Department of Experimental Medicine, University of Campania \u201cLuigi Vanvitelli\u201d, 80138 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1576-6290","authenticated-orcid":false,"given":"Massimiliano","family":"Galdiero","sequence":"additional","affiliation":[{"name":"Department of Experimental Medicine, University of Campania \u201cLuigi Vanvitelli\u201d, 80138 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,9,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e454","DOI":"10.1016\/S2214-109X(20)30037-1","article-title":"The burden of viral respiratory infections in young children in low-resource settings","volume":"8","author":"Okomo","year":"2020","journal-title":"Lancet Glob. 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