{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T10:42:51Z","timestamp":1780569771179,"version":"3.54.1"},"reference-count":60,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2023,9,10]],"date-time":"2023-09-10T00:00:00Z","timestamp":1694304000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001870","name":"Foundation for Polish Science","doi-asserted-by":"publisher","award":["POIR.04.04.00-00-16D7\/18-00"],"award-info":[{"award-number":["POIR.04.04.00-00-16D7\/18-00"]}],"id":[{"id":"10.13039\/501100001870","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001870","name":"Foundation for Polish Science","doi-asserted-by":"publisher","award":["MNiSW\/2020\/332\/DIR"],"award-info":[{"award-number":["MNiSW\/2020\/332\/DIR"]}],"id":[{"id":"10.13039\/501100001870","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Polish Ministry of Science and Higher Education","award":["POIR.04.04.00-00-16D7\/18-00"],"award-info":[{"award-number":["POIR.04.04.00-00-16D7\/18-00"]}]},{"name":"Polish Ministry of Science and Higher Education","award":["MNiSW\/2020\/332\/DIR"],"award-info":[{"award-number":["MNiSW\/2020\/332\/DIR"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Helicobacter pylori (H. pylori) infection is the most common cause of chronic gastritis, peptic ulcers and gastric cancer. Successful colonization of the stomach by H. pylori is related to the complex interactions of these bacteria and its components with host cells. The growing antibiotic resistance of H. pylori and various mechanisms of evading the immune response have forced the search for new biologically active substances that exhibit antibacterial properties and limit the harmful effects of these bacteria on gastric epithelial cells and immune cells. In this study, the usefulness of pyomelanin (PyoM) produced by Pseudomonas aeruginosa for inhibiting the metabolic activity of H. pylori was evaluated using the resazurin reduction assay, as well as in vitro cell studies used to verify the cytoprotective, anti-apoptotic and pro-regenerative effects of PyoM in the H. pylori LPS environment. We have shown that both water-soluble (PyoMsol) and water-insoluble (PyoMinsol) PyoM exhibit similar antibacterial properties against selected reference and clinical strains of H. pylori. This study showed that PyoM at a 1 \u03bcg\/mL concentration reduced H. pylori-driven apoptosis and reactive oxygen species (ROS) production in fibroblasts, monocytes or gastric epithelial cells. In addition, PyoM enhanced the phagocytosis of H. pylori. PyoMsol showed better pro-regenerative and immunomodulatory activities than PyoMinsol.<\/jats:p>","DOI":"10.3390\/ijms241813911","type":"journal-article","created":{"date-parts":[[2023,9,11]],"date-time":"2023-09-11T09:00:52Z","timestamp":1694422852000},"page":"13911","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Can Pyomelanin Produced by Pseudomonas aeruginosa Promote the Regeneration of Gastric Epithelial Cells and Enhance Helicobacter pylori Phagocytosis?"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2800-2054","authenticated-orcid":false,"given":"Mateusz M.","family":"Urbaniak","sequence":"first","affiliation":[{"name":"Department of Immunology and Infectious Biology, Faculty of Biology and Environmental Protection, University of \u0141\u00f3d\u017a, 90-237 \u0141\u00f3d\u017a, Poland"},{"name":"Bio-Med-Chem Doctoral School, University of Lodz and Lodz Institutes of the Polish Academy of Sciences, 90-237 \u0141\u00f3d\u017a, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3412-9000","authenticated-orcid":false,"given":"Karolina","family":"Rudnicka","sequence":"additional","affiliation":[{"name":"Department of Immunology and Infectious Biology, Faculty of Biology and Environmental Protection, University of \u0141\u00f3d\u017a, 90-237 \u0141\u00f3d\u017a, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6597-9609","authenticated-orcid":false,"given":"Gra\u017cyna","family":"Go\u015bciniak","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Medicine, Wroc\u0142aw Medical University, 50-368 Wroc\u0142aw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magdalena","family":"Chmiela","sequence":"additional","affiliation":[{"name":"Department of Immunology and Infectious Biology, Faculty of Biology and Environmental Protection, University of \u0141\u00f3d\u017a, 90-237 \u0141\u00f3d\u017a, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,9,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Baj, J., Forma, A., Sitarz, M., Piero, P., Garrut, G., Krasowska, D., and Maciejewski, R. 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