{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T02:04:07Z","timestamp":1780452247921,"version":"3.54.1"},"reference-count":55,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2023,9,13]],"date-time":"2023-09-13T00:00:00Z","timestamp":1694563200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Bridge to Life Limited","award":["2023-001"],"award-info":[{"award-number":["2023-001"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The global donor kidney shortage crisis has necessitated the use of suboptimal kidneys from donors-after-cardiac-death (DCD). Using an ex vivo porcine model of DCD kidney transplantation, the present study investigates whether the addition of hydrogen sulfide donor, AP39, to University of Wisconsin (UW) solution improves graft quality. Renal pedicles of male pigs were clamped in situ for 30 min and the ureters and arteries were cannulated to mimic DCD. Next, both donor kidneys were nephrectomized and preserved by static cold storage in UW solution with or without AP39 (200 nM) at 4 \u00b0C for 4 h followed by reperfusion with stressed autologous blood for 4 h at 37 \u00b0C using ex vivo pulsatile perfusion apparatus. Urine and arterial blood samples were collected hourly during reperfusion. After 4 h of reperfusion, kidneys were collected for histopathological analysis. Compared to the UW-only group, UW+AP39 group showed significantly higher pO2 (p &lt; 0.01) and tissue oxygenation (p &lt; 0.05). Also, there were significant increases in urine production and blood flow rate, and reduced levels of urine protein, serum creatinine, blood urea nitrogen, plasma Na+ and K+, as well as reduced intrarenal resistance in the UW+AP39 group compared to the UW-only group. Histologically, AP39 preserved renal structure by reducing the apoptosis of renal tubular cells and immune cell infiltration. Our finding could lay the foundation for improved graft preservation and reduce the increasingly poor outcomes associated with DCD kidney transplantation.<\/jats:p>","DOI":"10.3390\/ijms241814017","type":"journal-article","created":{"date-parts":[[2023,9,13]],"date-time":"2023-09-13T05:08:21Z","timestamp":1694581701000},"page":"14017","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Static Cold Storage with Mitochondria-Targeted Hydrogen Sulfide Donor Improves Renal Graft Function in an Ex Vivo Porcine Model of Controlled Donation-after-Cardiac-Death Kidney Transplantation"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8161-7202","authenticated-orcid":false,"given":"George J.","family":"Dugbartey","sequence":"first","affiliation":[{"name":"Matthew Mailing Center for Translational Transplant Studies, London Health Sciences Center, Western University, London, ON N6A 5C1, Canada"},{"name":"Department of Surgery, Division of Urology, London Health Sciences Center, Western University, London, ON N6A 5C1, Canada"},{"name":"Multi-Organ Transplant Program, London Health Sciences Center, Western University, London, ON N6A 5C1, Canada"},{"name":"Physiology & Pharmacology Department, Accra College of Medicine, Accra P.O. Box CT 9828, Ghana"},{"name":"Department of Pharmacology and Toxicology, School of Pharmacy, College of Health Sciences, University of Ghana, Legon, Accra P.O. Box LG43, Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Smriti","family":"Juriasingani","sequence":"additional","affiliation":[{"name":"Matthew Mailing Center for Translational Transplant Studies, London Health Sciences Center, Western University, London, ON N6A 5C1, Canada"},{"name":"Department of Microbiology & Immunology, Schulich School of Medicine & Dentistry, University of Western Ontario, London, ON N6A 5C1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5920-6511","authenticated-orcid":false,"given":"Mahms","family":"Richard-Mohamed","sequence":"additional","affiliation":[{"name":"Multi-Organ Transplant Program, London Health Sciences Center, Western University, London, ON N6A 5C1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew","family":"Rasmussen","sequence":"additional","affiliation":[{"name":"Department of Surgery, Division of Urology, London Health Sciences Center, Western University, London, ON N6A 5C1, Canada"},{"name":"Multi-Organ Transplant Program, London Health Sciences Center, Western University, London, ON N6A 5C1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Max","family":"Levine","sequence":"additional","affiliation":[{"name":"Department of Surgery, Division of Urology, London Health Sciences Center, Western University, London, ON N6A 5C1, Canada"},{"name":"Multi-Organ Transplant Program, London Health Sciences Center, Western University, London, ON N6A 5C1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Winnie","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Pathology & Laboratory Medicine, Western University, London, ON N6A 5C1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aaron","family":"Haig","sequence":"additional","affiliation":[{"name":"Department of Pathology & Laboratory Medicine, Western University, London, ON N6A 5C1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew","family":"Whiteman","sequence":"additional","affiliation":[{"name":"St. Luke\u2019s Campus, University of Exeter Medical School, Exeter EX1 2HZ, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacqueline","family":"Arp","sequence":"additional","affiliation":[{"name":"Matthew Mailing Center for Translational Transplant Studies, London Health Sciences Center, Western University, London, ON N6A 5C1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick P.W.","family":"Luke","sequence":"additional","affiliation":[{"name":"Matthew Mailing Center for Translational Transplant Studies, London Health Sciences Center, Western University, London, ON N6A 5C1, Canada"},{"name":"Department of Surgery, Division of Urology, London Health Sciences Center, Western University, London, ON N6A 5C1, Canada"},{"name":"Multi-Organ Transplant Program, London Health Sciences Center, Western University, London, ON N6A 5C1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alp","family":"Sener","sequence":"additional","affiliation":[{"name":"Matthew Mailing Center for Translational Transplant Studies, London Health Sciences Center, Western University, London, ON N6A 5C1, Canada"},{"name":"Department of Surgery, Division of Urology, London Health Sciences Center, Western University, London, ON N6A 5C1, Canada"},{"name":"Multi-Organ Transplant Program, London Health Sciences Center, Western University, London, ON N6A 5C1, Canada"},{"name":"Physiology & Pharmacology Department, Accra College of Medicine, Accra P.O. Box CT 9828, Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,9,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e4352","DOI":"10.1097\/MD.0000000000004352","article-title":"Superior outcomes of kidney transplantation compared with dialysis: An optimal matched analysis of a national population-based cohort study between 2005 and 2008 in Korea","volume":"95","author":"Yoo","year":"2016","journal-title":"Medicine"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"122","DOI":"10.2215\/CJN.04120416","article-title":"Dialysis Vintage and Outcomes after Kidney Transplantation: A Retrospective Cohort Study","volume":"12","author":"Haller","year":"2017","journal-title":"Clin. J. Am. Soc. Nephrol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"e2215217","DOI":"10.1001\/jamanetworkopen.2022.15217","article-title":"Effect of Perioperative Dexmedetomidine on Delayed Graft Function Following a Donation-After-Cardiac-Death Kidney Transplant: A Randomized Clinical Trial","volume":"5","author":"Shan","year":"2022","journal-title":"JAMA Netw. Open."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/j.transproceed.2019.10.001","article-title":"Comparison of Graft Outcome Between Donation After Circulatory Death and Living-Donor Kidney Transplantation","volume":"52","author":"Zhang","year":"2020","journal-title":"Transplant. Proc."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"999","DOI":"10.1097\/XCS.0000000000000174","article-title":"Anastomosis Time and Outcomes after Donation after Circulatory Death Kidney Transplantation","volume":"234","author":"Cron","year":"2022","journal-title":"J. Am. Coll. Surg."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1111\/iju.13851","article-title":"Warm ischemic time as a critical risk factor of graft failure from donors after cardiac death: A single-center experience over three decades in the Kidney Donor Profile Index\/Kidney Donor Risk Index era in Japan","volume":"26","author":"Kusaka","year":"2019","journal-title":"Int. J. Urol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"843","DOI":"10.1111\/ajt.14523","article-title":"CD47 blockade reduces ischemia\/reperfusion injury in donation after cardiac death rat kidney transplantation","volume":"18","author":"Wang","year":"2018","journal-title":"Am. J. Transplant."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"733","DOI":"10.1016\/j.transproceed.2023.03.008","article-title":"Total Cell-Free DNA as a Noninvasive Biomarker of a Delayed Graft Function After Kidney Transplantation from Donors After Cardiac Death","volume":"55","author":"Kusaka","year":"2023","journal-title":"Transplant. Proc."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.niox.2022.05.005","article-title":"Nitric oxide and long-term outcomes after kidney transplantation: Results of the Transplant Lines cohort study","volume":"125\u2013126","author":"Maassen","year":"2022","journal-title":"Nitric Oxide"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"468","DOI":"10.1038\/labinvest.2016.157","article-title":"High-pressure carbon monoxide preserves rat kidney grafts from apoptosis and inflammation","volume":"97","author":"Abe","year":"2017","journal-title":"Lab. Investig."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"105883","DOI":"10.1016\/j.phrs.2021.105883","article-title":"Application of carbon monoxide in kidney and heart transplantation: A novel pharmacological strategy for a broader use of suboptimal renal and cardiac grafts","volume":"173","author":"Dugbartey","year":"2021","journal-title":"Pharmacol. Res."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"941374","DOI":"10.3389\/fcvm.2022.941374","article-title":"Bibliometric evaluation of 2011\u20132021 publications on hydrogen sulfide in heart preservation research","volume":"9","author":"Chen","year":"2023","journal-title":"Front. Cardiovasc. Med."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Juriasingani, S., Jackson, A., Zhang, M.Y., Ruthirakanthan, A., Dugbartey, G.J., Sogutdelen, E., Levine, M., Mandurah, M., Whiteman, M., and Luke, P. (2021). Evaluating the Effects of Subnormothermic Perfusion with AP39 in a Novel Blood-Free Model of Ex Vivo Kidney Preservation and Reperfusion. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22137180"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"532127","DOI":"10.1155\/2011\/532127","article-title":"Contribution of large pig for renal ischemia-reperfusion and transplantation studies: The preclinical model","volume":"2011","author":"Giraud","year":"2011","journal-title":"J. Biomed. Biotechnol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"4864","DOI":"10.2741\/2434","article-title":"The importance of large animal models in transplantation","volume":"12","author":"Dehoux","year":"2007","journal-title":"Front. Biosci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1111\/ajt.14080","article-title":"Hydrogen Sulfide Protects Renal Grafts Against Prolonged Cold Ischemia-Reperfusion Injury via Specific Mitochondrial Actions","volume":"17","author":"Lobb","year":"2017","journal-title":"Am. J. Transplant."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"2698","DOI":"10.1111\/ajt.16401","article-title":"Preservation of pancreas in the University of Wisconsin solution supplemented with AP39 reduces reactive oxygen species production and improves islet graft function","volume":"21","author":"Nishime","year":"2021","journal-title":"Am. J. Transplant."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"e25","DOI":"10.1016\/j.jss.2012.09.028","article-title":"Inhibition of hydrogen sulfide generation contributes to lung injury after experimental orthotopic lung transplantation","volume":"182","author":"Wu","year":"2013","journal-title":"J. Surg. Res."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1173","DOI":"10.1046\/j.1523-1755.2001.0600031173.x","article-title":"Prolonged cold preservation augments vascular injury independent of renal transplant immunogenicity and function","volume":"60","author":"Dragun","year":"2001","journal-title":"Kidney Int."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"713","DOI":"10.1111\/j.1523-1755.2004.00416.x","article-title":"Cold ischemia and the reduced long-term survival of cadaveric renal allografts","volume":"65","author":"Salahudeen","year":"2004","journal-title":"Kidney Int."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1689","DOI":"10.1093\/ndt\/gfl042","article-title":"Major effects of delayed graft function and cold ischaemia time on renal allograft survival","volume":"21","author":"Quiroga","year":"2006","journal-title":"Nephrol. Dial. Transplant."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"2647","DOI":"10.1111\/j.1600-6143.2011.03741.x","article-title":"Impact of cold ischemia time on graft survival among ECD transplant recipients: A paired kidney analysis","volume":"11","author":"Kayler","year":"2011","journal-title":"Am. J. Transplant."},{"key":"ref_23","first-page":"H743","article-title":"Mitochondrial complexes I, II, III, IV, and V in myocardial ischemia and autolysis","volume":"244","author":"Rouslin","year":"1983","journal-title":"Am. J. Physiol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"15560","DOI":"10.1073\/pnas.0705891104","article-title":"Hydrogen sulfide attenuates myocardial ischemia-reperfusion injury by preservation of mitochondrial function","volume":"104","author":"Elrod","year":"2007","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1089\/ars.2008.2282","article-title":"Hydrogen sulfide increases glutathione production and suppresses oxidative stress in mitochondria","volume":"12","author":"Kimura","year":"2010","journal-title":"Antioxid. Redox Signal."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1124\/mol.108.047985","article-title":"Hydrogen sulfide inhibits rotenone-induced apoptosis via preservation of mitochondrial function","volume":"75","author":"Hu","year":"2009","journal-title":"Mol. Pharmacol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1016\/0006-291X(89)91632-X","article-title":"Mitochondrial and xanthine oxidase both generate reactive oxygen species in isolated perfused rat liver after hypoxic injury","volume":"160","author":"Jaeschke","year":"1989","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"2929","DOI":"10.1074\/jbc.271.6.2929","article-title":"Effect of inorganic phosphate concentration on nature of inner mitochondrial membrane alterations mediated by Ca2+ ions","volume":"271","author":"Kowaltowski","year":"1996","journal-title":"J. Biol. Chem."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"C1783","DOI":"10.1152\/ajpcell.1997.273.6.C1783","article-title":"Mitochondrial permeability transition in pH-dependent reperfusion injury to rat hepatocytes","volume":"273","author":"Qian","year":"1997","journal-title":"Am. J. Physiol."},{"key":"ref_30","first-page":"85","article-title":"Effect of brain death and non-heart-beating kidney donation on renal function and injury: An assessment in the isolated perfused rat kidney","volume":"1","author":"Leuvenink","year":"2003","journal-title":"Exp. Clin. Transplant."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1006\/jsre.1996.0363","article-title":"Mitochondrial glutathione redox and energy producing function during liver ischemia and reperfusion","volume":"66","author":"Kurokawa","year":"1996","journal-title":"J. Surg. Res."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1416","DOI":"10.1097\/00007890-199605150-00026","article-title":"Glyoxalase II and glutathione levels in rat liver mitochondria during cold storage in Euro-Collins and University of Wisconsin solutions","volume":"61","author":"Jassem","year":"1996","journal-title":"Transplantation"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"698","DOI":"10.1038\/nm0698-698","article-title":"Redox gene therapy for ischemia\/reperfusion injury of the liver reduces AP1 and NF-kB activation","volume":"4","author":"Zwacka","year":"1998","journal-title":"Nat. Med."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1002\/bjs.6856","article-title":"Hydrogen sulphide ameliorates ischaemia-reperfusion injury in an experimental model of non-heart-beating donor kidney transplantation","volume":"97","author":"Hosgood","year":"2010","journal-title":"Br. J. Surg."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Maassen, H., Hendriks, K.D.W., Venema, L.H., Henning, R.H., Hofker, S.H., van Goor, H., Leuvenink, H.G.D., and Coester, A.M. (2019). Hydrogen sulfide-induced hypometabolism in human-sized porcine kidneys. PLoS ONE, 14.","DOI":"10.1371\/journal.pone.0225152"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Ertugrul, I.A., van Suylen, V., Damman, K., de Koning, M.L.Y., van Goor, H., and Erasmus, M.E. (2021). Donor Heart Preservation with Hydrogen Sulfide: A Systematic Review and Meta-Analysis. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22115737"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"203","DOI":"10.7555\/JBR.28.20140037","article-title":"GYY4137 protects against myocardial ischemia and reperfusion injury by attenuating oxidative stress and apoptosis in rats","volume":"29","author":"Meng","year":"2015","journal-title":"J. Biomed. Res."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1016\/j.ejphar.2015.11.022","article-title":"Dopamine treatment attenuates acute kidney injury in a rat model of deep hypothermia and rewarming\u2014The role of renal H2S-producing enzymes","volume":"769","author":"Dugbartey","year":"2015","journal-title":"Eur. J. Pharmacol."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Corsello, T., Komaravelli, N., and Casola, A. (2018). Role of Hydrogen Sulfide in NRF2- and Sirtuin-Dependent Maintenance of Cellular Redox Balance. Antioxidants, 7.","DOI":"10.3390\/antiox7100129"},{"key":"ref_40","first-page":"186","article-title":"The novel mitochondria-targeted hydrogen sulfide (H2S) donors AP123 and AP39 protect against hyperglycemic injury in microvascular endothelial cells in vitro","volume":"113 Pt A","author":"Torregrossa","year":"2016","journal-title":"Pharmacol. Res."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1042\/bj3410233","article-title":"The mitochondrial permeability transition pore and its role in cell death","volume":"341","author":"Crompton","year":"1999","journal-title":"Biochem. J."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1111\/bph.13688","article-title":"AP39, a mitochondria-targeting hydrogen sulfide (H2S) donor, protects against myocardial reperfusion injury independently of salvage kinase signalling","volume":"174","author":"Karwi","year":"2017","journal-title":"Br. J. Pharmacol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"E269","DOI":"10.1152\/ajpendo.00251.2018","article-title":"Hydrogen sulfide inhibits Ca2+-induced mitochondrial permeability transition pore opening in type-1 diabetes","volume":"317","author":"Kundu","year":"2019","journal-title":"Am. J. Physiol. Endocrinol. Metab."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"3","DOI":"10.15407\/fz59.01.003","article-title":"Hydrogen sulfide inhibits Ca(2+)-induced mitochondrial permeability transition pore opening in spontaneously hypertensive rats","volume":"59","author":"Dorofeieva","year":"2013","journal-title":"Fiziol. Zhurnal"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"3","DOI":"10.15407\/fz57.04.003","article-title":"Hydrogen sulfide inhibits Ca(2+)-induced mitochondrial permeability transition pore opening in adult and old rat heart","volume":"57","author":"Semenykhina","year":"2011","journal-title":"Fiziol. Zhurnal"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1159\/000149797","article-title":"Hypothermic injury: The mitochondrial calcium, ATP and ROS love-hate triangle out of balance","volume":"22","author":"Brinkkoetter","year":"2008","journal-title":"Cell. Physiol. Biochem."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1006\/cryo.1997.2055","article-title":"The effect of calcium on hypothermia-facilitated resuscitation of warm ischemic kidney tissue slices: A role for the mitochondrial permeability transition pore?","volume":"36","author":"McAnulty","year":"1998","journal-title":"Cryobiology"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"3139","DOI":"10.1111\/ajt.15539","article-title":"Supplementing preservation solution with mitochondria-targeted H2S donor AP39 protects cardiac grafts from prolonged cold ischemia-reperfusion injury in heart transplantation","volume":"19","author":"Zhu","year":"2019","journal-title":"Am. J. Transplant."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"e13411","DOI":"10.1111\/petr.13411","article-title":"Plasma electrolyte imbalance in pediatric kidney transplant recipients","volume":"23","author":"Hayes","year":"2019","journal-title":"Pediatr. Transplant."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1056","DOI":"10.1124\/jpet.108.149963","article-title":"Production and actions of hydrogen sulfide, a novel gaseous bioactive substance, in the kidneys","volume":"329","author":"Xia","year":"2009","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1038\/ajh.2011.149","article-title":"Interdependency of cystathione \u03b3-lyase and cystathione \u03b2-synthase in hydrogen sulfide-induced blood pressure regulation in rats","volume":"25","author":"Roy","year":"2012","journal-title":"Am. J. Hypertens."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"2061","DOI":"10.1089\/ars.2013.5304","article-title":"Hydrogen sulfide targets EGFR Cys797\/Cys798 residues to induce Na(+)\/K(+)-ATPase endocytosis and inhibition in renal tubular epithelial cells and increase sodium excretion in chronic salt-loaded rats","volume":"21","author":"Ge","year":"2014","journal-title":"Antioxid. Redox Signal."},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Zhang, J., Chen, S., Liu, H., Zhang, B., Zhao, Y., Ma, K., Zhao, D., Wang, Q., Ma, H., and Zhang, Z. (2013). Hydrogen sulfide prevents hydrogen peroxide-induced activation of epithelial sodium channel through a PTEN\/PI(3,4,5)P3 dependent pathway. PLoS ONE, 8.","DOI":"10.1371\/annotation\/1cc3a25b-dc35-4d2a-8465-b11916f2e863"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"112435","DOI":"10.1016\/j.biopha.2021.112435","article-title":"Sodium thiosulfate-supplemented UW solution protects renal grafts against prolonged cold ischemia-reperfusion injury in a murine model of syngeneic kidney transplantation","volume":"145","author":"Zhang","year":"2022","journal-title":"Biomed. Pharmacother."},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Juriasingani, S., Ruthirakanthan, A., Richard-Mohamed, M., Akbari, M., Aquil, S., Patel, S., Al-Ogaili, R., Whiteman, M., Luke, P., and Sener, A. (2021). Subnormothermic Perfusion with H2S Donor AP39 Improves DCD Porcine Renal Graft Outcomes in an Ex Vivo Model of Kidney Preservation and Reperfusion. Biomolecules, 11.","DOI":"10.3390\/biom11030446"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/18\/14017\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T20:49:49Z","timestamp":1760129389000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/18\/14017"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,9,13]]},"references-count":55,"journal-issue":{"issue":"18","published-online":{"date-parts":[[2023,9]]}},"alternative-id":["ijms241814017"],"URL":"https:\/\/doi.org\/10.3390\/ijms241814017","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,9,13]]}}}