{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:48:40Z","timestamp":1787028520868,"version":"build-2736575974"},"reference-count":27,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2023,9,14]],"date-time":"2023-09-14T00:00:00Z","timestamp":1694649600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>This study aimed to investigate the antibiofilm and remineralising effects of peptide GAPI on artificial dentin caries. After creating artificial carious lesions, eighty dentine blocks were randomly assigned for treatment twice daily with GAPI (GAPI group) or deionised water (control group). Both groups underwent a 7-day biochemical cycle. Scanning electron microscopy (SEM) showed S. mutans with damaged structures that partially covered the dentine in the GAPI group. The dead\u2013live ratios for the GAPI and control groups were 0.77 \u00b1 0.13 and 0.37 \u00b1 0.09 (p &lt; 0.001). The log colony-forming units for the GAPI and control groups were 7.45 \u00b1 0.32 and 8.74 \u00b1 0.50 (p &lt; 0.001), respectively. The lesion depths for the GAPI and control groups were 151 \u00b1 18 \u00b5m and 214 \u00b1 15 \u00b5m (p &lt; 0.001), respectively. The mineral losses for the GAPI and control groups were 0.91 \u00b1 0.07 gHAcm\u22123 and 1.01 \u00b1 0.07 gHAcm\u22123 (p = 0.01), respectively. The hydrogen-to-amide I ratios for the GAPI and control groups were 2.92 \u00b1 0.82 and 1.83 \u00b1 0.73 (p = 0.014), respectively. SEM micrographs revealed fewer exposed dentine collagen fibres in the GAPI group compared to those in the control group. Furthermore, X-ray diffraction (XRD) patterns indicated that the hydroxyapatite in the GAPI group was more crystallised than that in the control group. This study demonstrated GAPI\u2019s antibiofilm and remineralising effects on artificial dentin caries.<\/jats:p>","DOI":"10.3390\/ijms241814076","type":"journal-article","created":{"date-parts":[[2023,9,14]],"date-time":"2023-09-14T09:57:56Z","timestamp":1694685476000},"page":"14076","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["The Anti-Caries Effects of a Novel Peptide on Dentine Caries: An In Vitro Study"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3437-6060","authenticated-orcid":false,"given":"Olivia Lili","family":"Zhang","sequence":"first","affiliation":[{"name":"Faculty of Dentistry, The University of Hong Kong, Hong Kong 999077, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3471-180X","authenticated-orcid":false,"given":"John Yun","family":"Niu","sequence":"additional","affiliation":[{"name":"Faculty of Dentistry, The University of Hong Kong, Hong Kong 999077, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5310-5039","authenticated-orcid":false,"given":"Ollie Yiru","family":"Yu","sequence":"additional","affiliation":[{"name":"Faculty of Dentistry, The University of Hong Kong, Hong Kong 999077, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1167-2999","authenticated-orcid":false,"given":"Iris Xiaoxue","family":"Yin","sequence":"additional","affiliation":[{"name":"Faculty of Dentistry, The University of Hong Kong, Hong Kong 999077, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7745-257X","authenticated-orcid":false,"given":"May Lei","family":"Mei","sequence":"additional","affiliation":[{"name":"Faculty of Dentistry, The University of Hong Kong, Hong Kong 999077, China"},{"name":"Faculty of Dentistry, The University of Otago, Dunedin 9054, New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8167-0430","authenticated-orcid":false,"given":"Chun Hung","family":"Chu","sequence":"additional","affiliation":[{"name":"Faculty of Dentistry, The University of Hong Kong, Hong Kong 999077, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,9,14]]},"reference":[{"key":"ref_1","first-page":"10","article-title":"Dental caries: An infectious and transmissible disease","volume":"26","author":"Caufield","year":"2005","journal-title":"Compend. 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