{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T23:26:18Z","timestamp":1773789978506,"version":"3.50.1"},"reference-count":62,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2023,9,15]],"date-time":"2023-09-15T00:00:00Z","timestamp":1694736000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Russian Science Foundation","award":["20-14-00148-II"],"award-info":[{"award-number":["20-14-00148-II"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Numerous studies have demonstrated that people with type 2 diabetes mellitus (associated with IAPP peptide aggregation) show an increased incidence of Alzheimer\u2019s disease (associated with A\u03b2 aggregation), but the mechanism responsible for this correlation is presently unknown. Here, we applied a yeast-based model to study the interactions of IAPP with PrP (associated with TSEs) and with the A\u03b242 peptide. We demonstrated that fluorescently tagged IAPP forms detergent-resistant aggregates in yeast cells. Using the FRET approach, we showed that IAPP and A\u03b2 aggregates co-localize and physically interact in yeast cells. We also showed that this interaction is specific and that there is no interaction between IAPP and PrP in the yeast system. Our data confirmed a direct physical interaction between IAPP and A\u03b242 aggregates in a living cell. Based on these findings, we hypothesize that this interaction may play a crucial role in seeding A\u03b242 aggregation in T2DM patients, thereby promoting the development of AD.<\/jats:p>","DOI":"10.3390\/ijms241814122","type":"journal-article","created":{"date-parts":[[2023,9,15]],"date-time":"2023-09-15T02:54:13Z","timestamp":1694746453000},"page":"14122","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["The A\u03b242 Peptide and IAPP Physically Interact in a Yeast-Based Assay"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2279-8514","authenticated-orcid":false,"given":"Daniel V.","family":"Kachkin","sequence":"first","affiliation":[{"name":"Laboratory of Amyloid Biology, St. Petersburg State University, St. Petersburg 199034, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-1972-2355","authenticated-orcid":false,"given":"Veronika V.","family":"Lashkul","sequence":"additional","affiliation":[{"name":"Laboratory of Amyloid Biology, St. Petersburg State University, St. Petersburg 199034, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Natalia A.","family":"Gorsheneva","sequence":"additional","affiliation":[{"name":"Laboratory of Amyloid Biology, St. Petersburg State University, St. Petersburg 199034, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7428-120X","authenticated-orcid":false,"given":"Sergey A.","family":"Fedotov","sequence":"additional","affiliation":[{"name":"Laboratory of Amyloid Biology, St. Petersburg State University, St. Petersburg 199034, Russia"},{"name":"Pavlov Institute of Physiology, Russian Academy of Sciences, St. Petersburg 199034, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria S.","family":"Rubel","sequence":"additional","affiliation":[{"name":"Laboratory of DNA-Nanosensor Diagnostics, SCAMT Institute, ITMO University, St. Petersburg 191002, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8934-9051","authenticated-orcid":false,"given":"Yury O.","family":"Chernoff","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, Georgia Institute of Technology, Atlanta, GA 30332, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6203-2006","authenticated-orcid":false,"given":"Aleksandr A.","family":"Rubel","sequence":"additional","affiliation":[{"name":"Laboratory of Amyloid Biology, St. Petersburg State University, St. Petersburg 199034, Russia"},{"name":"Pediatric Research and Clinical Center for Infectious Diseases, Department of Medical Microbiology and Molecular Epidemiology, St. Petersburg 197022, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,9,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1080\/13506129.2018.1549825","article-title":"Amyloid nomenclature 2018: Recommendations by the International Society of Amyloidosis (ISA) nomenclature committee","volume":"4","author":"Benson","year":"2018","journal-title":"Amyloid"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1080\/13506129.2022.2147636","article-title":"Amyloid nomenclature 2022: Update, novel proteins, and recommendations by the International Society of Amyloidosis (ISA) Nomenclature Committee","volume":"29","author":"Buxbaum","year":"2022","journal-title":"Amyloid"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1016\/0165-6147(91)90609-V","article-title":"Amyloid Deposition as the Central Event in the Aetiology of Alzheimer\u2019s Disease","volume":"12","author":"Hardy","year":"1991","journal-title":"Trends Pharmacol. 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