{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,30]],"date-time":"2025-12-30T08:52:54Z","timestamp":1767084774503,"version":"build-2065373602"},"reference-count":76,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2023,9,21]],"date-time":"2023-09-21T00:00:00Z","timestamp":1695254400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000968","name":"American Heart Association","doi-asserted-by":"publisher","award":["AHAPOST903757","RO1 HL70562-13S1","UC\u2013HBCU","RO1HL70562"],"award-info":[{"award-number":["AHAPOST903757","RO1 HL70562-13S1","UC\u2013HBCU","RO1HL70562"]}],"id":[{"id":"10.13039\/100000968","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000009","name":"National Institute of Health","doi-asserted-by":"publisher","award":["AHAPOST903757","RO1 HL70562-13S1","UC\u2013HBCU","RO1HL70562"],"award-info":[{"award-number":["AHAPOST903757","RO1 HL70562-13S1","UC\u2013HBCU","RO1HL70562"]}],"id":[{"id":"10.13039\/100000009","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008476","name":"University of California\u2013Historically Black Colleges and Universities","doi-asserted-by":"publisher","award":["AHAPOST903757","RO1 HL70562-13S1","UC\u2013HBCU","RO1HL70562"],"award-info":[{"award-number":["AHAPOST903757","RO1 HL70562-13S1","UC\u2013HBCU","RO1HL70562"]}],"id":[{"id":"10.13039\/100008476","id-type":"DOI","asserted-by":"publisher"}]},{"name":"National Institutes of Health","award":["AHAPOST903757","RO1 HL70562-13S1","UC\u2013HBCU","RO1HL70562"],"award-info":[{"award-number":["AHAPOST903757","RO1 HL70562-13S1","UC\u2013HBCU","RO1HL70562"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Endogenous hydrogen sulfide (H2S) produced by cystathionine \u03b2-synthase (CBS) and cystathionine-\u03b3 lyase (CSE) has emerged as a novel uterine vasodilator contributing to pregnancy-associated increases in uterine blood flow, which safeguard pregnancy health. Uterine artery (UA) H2S production is stimulated via exogenous estrogen replacement and is associated with elevated endogenous estrogens during pregnancy through the selective upregulation of CBS without altering CSE. However, how endogenous estrogens regulate uterine artery CBS expression in pregnancy is unknown. This study was conducted to test a hypothesis that endogenous estrogens selectively stimulate UA CBS expression via specific estrogen receptors (ER). Treatment with E2\u03b2 (0.01 to 100 nM) stimulated CBS but not CSE mRNA in organ cultures of fresh UA rings from both NP and P (gestational day 20, GD20) rats, with greater responses to all doses of E2\u03b2 tested in P vs. NP UA. ER antagonist ICI 182,780 (ICI, 1 \u00b5M) completely attenuated E2\u03b2-stimulated CBS mRNA in both NP and P rat UA. Subcutaneous injection with ICI 182,780 (0.3 mg\/rat) of GD19 P rats for 24 h significantly inhibited UA CBS but not mRNA expression, consistent with reduced endothelial and smooth muscle cell CBS (but not CSE) protein. ICI did not alter mesenteric and renal artery CBS and CSE mRNA. In addition, ICI decreased endothelial nitric oxide synthase mRNA in UA but not in mesenteric or renal arteries. Thus, pregnancy-augmented UA CBS\/H2S production is mediated by the actions of endogenous estrogens via specific ER in pregnant rats.<\/jats:p>","DOI":"10.3390\/ijms241814384","type":"journal-article","created":{"date-parts":[[2023,9,21]],"date-time":"2023-09-21T21:08:36Z","timestamp":1695330516000},"page":"14384","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["ICI 182,780 Attenuates Selective Upregulation of Uterine Artery Cystathionine \u03b2-Synthase Expression in Rat Pregnancy"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4241-8814","authenticated-orcid":false,"given":"Jin","family":"Bai","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology, University of California Irvine, Irvine, CA 92697, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yao","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Laboratory Animal Sciences, School of Medicine, Shanghai Jiaotong University, 280 South Chongqing Road, Shanghai 200025, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guofeng","family":"Yan","sequence":"additional","affiliation":[{"name":"Department of Laboratory Animal Sciences, School of Medicine, Shanghai Jiaotong University, 280 South Chongqing Road, Shanghai 200025, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Laboratory Animal Sciences, School of Medicine, Shanghai Jiaotong University, 280 South Chongqing Road, Shanghai 200025, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alejandra Garcia","family":"Salmeron","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, University of California Irvine, Irvine, CA 92697, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olamide Tolulope","family":"Fategbe","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, University of California Irvine, Irvine, CA 92697, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sathish","family":"Kumar","sequence":"additional","affiliation":[{"name":"Department of Comparative Biosciences, University of Wisconsin-Madison, Madison, WI 53706, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuejin","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Laboratory Animal Sciences, School of Medicine, Shanghai Jiaotong University, 280 South Chongqing Road, Shanghai 200025, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1067-6018","authenticated-orcid":false,"given":"Dong-Bao","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, University of California Irvine, Irvine, CA 92697, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,9,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/S0146-0005(00)80047-6","article-title":"Hemodynamic changes in pregnancy","volume":"24","author":"Thornburg","year":"2000","journal-title":"Semin. Perinatol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1003","DOI":"10.1161\/CIRCULATIONAHA.114.009029","article-title":"Cardiovascular physiology of pregnancy","volume":"130","author":"Sanghavi","year":"2014","journal-title":"Circulation"},{"key":"ref_3","first-page":"H231","article-title":"Distribution of cardiac output in ovine pregnancy","volume":"232","author":"Rosenfeld","year":"1977","journal-title":"Am. J. Physiol."},{"key":"ref_4","first-page":"1000","article-title":"Quantitative estimation of human uterine artery blood flow and pelvic blood flow redistribution in pregnancy","volume":"80","author":"Palmer","year":"1992","journal-title":"Obstet. Gynecol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1592","DOI":"10.1126\/science.1111726","article-title":"Latest advances in understanding preeclampsia","volume":"308","author":"Redman","year":"2005","journal-title":"Science"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1690","DOI":"10.1016\/j.jacc.2020.08.014","article-title":"Preeclampsia\u2014Pathophysiology and Clinical Presentations","volume":"76","author":"Ives","year":"2020","journal-title":"J. Am. Coll. Cardiol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"S844","DOI":"10.1016\/j.ajog.2021.11.1356","article-title":"The etiology of preeclampsia","volume":"226","author":"Jung","year":"2022","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1056\/NEJMra0708473","article-title":"Effect of in utero and early-life conditions on adult health and disease","volume":"359","author":"Gluckman","year":"2008","journal-title":"N. Engl. J. Med."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1210\/edrv-11-1-124","article-title":"Placental steroid hormone biosynthesis in primate pregnancy","volume":"11","author":"Albrecht","year":"1990","journal-title":"Endocr. Rev."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Parisi, F., Fenizia, C., Introini, A., Zavatta, A., Scaccabarozzi, C., Biasin, M., and Savasi, V. (2023). The pathophysiological role of estrogens in the initial stages of pregnancy: Molecular mechanisms and clinical implications for pregnancy outcome from the periconceptional period to end of the first trimester. Hum. Reprod. Updat., dmad016.","DOI":"10.1093\/humupd\/dmad016"},{"key":"ref_11","first-page":"E536","article-title":"Local and systemic estradiol-17 beta: Effects on uterine and systemic vasodilation","volume":"256","author":"Magness","year":"1989","journal-title":"Am. J. Physiol."},{"key":"ref_12","first-page":"H731","article-title":"Systemic and uterine blood flow distribution during prolonged infusion of 17beta-estradiol","volume":"275","author":"Magness","year":"1998","journal-title":"Am. J. Physiol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1113\/jphysiol.2005.086439","article-title":"Uterine blood flow responses to ICI 182 780 in ovariectomized oestradiol-17beta-treated, intact follicular and pregnant sheep","volume":"565","author":"Magness","year":"2005","journal-title":"J. Physiol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1210\/er.2016-1065","article-title":"From pregnancy to preeclampsia: A key role for estrogens","volume":"38","author":"Berkane","year":"2017","journal-title":"Endocr. Rev."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"H1692","DOI":"10.1152\/ajpheart.2001.280.4.H1692","article-title":"Endothelial vasodilator production by uterine and systemic arteries. VI. Ovarian and pregnancy effects on eNOS and NO(x)","volume":"280","author":"Magness","year":"2001","journal-title":"Am. J. Physiol. Heart Circ. Physiol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"H1699","DOI":"10.1152\/ajpheart.2001.280.4.H1699","article-title":"Endothelial vasodilator production by uterine and systemic arteries. VII. Estrogen and progesterone effects on eNOS","volume":"280","author":"Rupnow","year":"2001","journal-title":"Am. J. Physiol. Heart Circ. Physiol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"406","DOI":"10.1161\/01.RES.87.5.406","article-title":"Increased nitric oxide synthase activity and expression in the human uterine artery during pregnancy","volume":"87","author":"Nelson","year":"2000","journal-title":"Circ. Res."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1210\/en.2003-0547","article-title":"Membrane estrogen receptor-dependent extracellular signal-regulated kinase pathway mediates acute activation of endothelial nitric oxide synthase by estrogen in uterine artery endothelial cells","volume":"145","author":"Chen","year":"2004","journal-title":"Endocrinology"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"2158","DOI":"10.1172\/JCI119022","article-title":"Nitric oxide contributes to estrogen-induced vasodilation of the ovine uterine circulation","volume":"98","author":"Rosenfeld","year":"1996","journal-title":"J. Clin. Investig."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"828","DOI":"10.1016\/S0002-9378(11)91597-X","article-title":"Estrogen-induced uterine vasodilatation is antagonized by L-nitroarginine methyl ester, an inhibitor of nitric oxide synthesis","volume":"167","author":"Yang","year":"1992","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1016\/j.mehy.2017.08.003","article-title":"Phosphodiesterases and preeclampsia","volume":"108","author":"Larre","year":"2017","journal-title":"Med Hypotheses"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1097\/AOG.0000000000001518","article-title":"Perinatal and hemodynamic evaluation of Sildenafil Citrate for preeclampsia treatment: A randomized controlled trial","volume":"128","author":"Trapani","year":"2016","journal-title":"Obstet. Gynecol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/S2352-4642(17)30173-6","article-title":"Maternal sildenafil for severe fetal growth restriction (STRIDER): A multicentre, randomised, placebo-controlled, double-blind trial","volume":"2","author":"Sharp","year":"2018","journal-title":"Lancet Child Adolesc. Health"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"21972","DOI":"10.1073\/pnas.0908047106","article-title":"Hydrogen sulfide is an endogenous stimulator of angiogenesis","volume":"106","author":"Papapetropoulos","year":"2009","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1126\/science.1162667","article-title":"H2S as a physiologic vasorelaxant: Hypertension in mice with deletion of cystathionine gamma-lyase","volume":"322","author":"Yang","year":"2008","journal-title":"Science"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"2288","DOI":"10.1210\/en.2015-1086","article-title":"Estrogen replacement therapy in ovariectomized nonpregnant ewes stimulates uterine artery hydrogen sulfide biosynthesis by selectively up-regulating cystathionine beta-synthase expression","volume":"156","author":"Lechuga","year":"2015","journal-title":"Endocrinology"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"514","DOI":"10.1093\/biolre\/ioy207","article-title":"E2beta stimulates ovine uterine artery endothelial cell H2S production in vitro by estrogen receptor-dependent upregulation of cystathionine beta-synthase and cystathionine gamma-lyase expression","volume":"100","author":"Lechuga","year":"2019","journal-title":"Biol. Reprod."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"664","DOI":"10.1095\/biolreprod.116.143834","article-title":"Augmented H2S production via cystathionine-beta-synthase upregulation plays a role in pregnancy-associated uterine vasodilation","volume":"96","author":"Sheibani","year":"2017","journal-title":"Biol. Reprod."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1630","DOI":"10.1093\/biolre\/ioz027","article-title":"Ovine uterine artery hydrogen sulfide biosynthesis in vivo: Effects of ovarian cycle and pregnancydagger","volume":"100","author":"Lechuga","year":"2019","journal-title":"Biol. Reprod."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Li, Y., Bai, J., Yang, Y.H., Hoshi, N., and Chen, D.B. (2020). Hydrogen sulfide relaxes human uterine artery via activating smooth muscle BKCa channels. Antioxidants, 9.","DOI":"10.3390\/antiox9111127"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"H422","DOI":"10.1152\/ajpheart.2001.281.1.H422","article-title":"Ca2+-activated K+ channels modulate basal and E2beta-induced rises in uterine blood flow in ovine pregnancy","volume":"281","author":"Rosenfeld","year":"2001","journal-title":"Am. J. Physiol. Heart Circ. Physiol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"6012","DOI":"10.1210\/en.2012-1717","article-title":"Large conductance Ca2+-activated and voltage-activated K+ channels contribute to the rise and maintenance of estrogen-induced uterine vasodilation and maintenance of blood pressure","volume":"153","author":"Rosenfeld","year":"2012","journal-title":"Endocrinology"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"9264","DOI":"10.1002\/jcp.27606","article-title":"Estradiol-17beta stimulates H2S biosynthesis by ER-dependent CBS and CSE transcription in uterine artery smooth muscle cells in vitro","volume":"234","author":"Lechuga","year":"2019","journal-title":"J. Cell Physiol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1109445","DOI":"10.1530\/JME-22-0175","article-title":"ERalpha\/ERbeta-directed CBS transcription mediates E2beta-stimulated hUAEC H2S production","volume":"70","author":"Bai","year":"2023","journal-title":"J. Mol. Endocrinol."},{"key":"ref_35","first-page":"507","article-title":"Maternal cardiovascular and other physiologic responses to the endocrinology of pregnancy","volume":"Volume 9","author":"Bazer","year":"1998","journal-title":"Endocrinology of Pregnancy"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1095\/biolreprod59.4.933","article-title":"Regulation of inducible nitric oxide synthase messenger ribonucleic acid expression in pregnant rat uterus","volume":"59","author":"Dong","year":"1998","journal-title":"Biol. Reprod."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1152\/ajplegacy.1932.100.1.32","article-title":"Rhythmic vascular uterine changes","volume":"100","author":"Markee","year":"1932","journal-title":"Am. J. Physiol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"829","DOI":"10.1016\/S0002-9378(16)34033-9","article-title":"Effect of ovarian hormones on the uterine vascular bed","volume":"107","author":"Greiss","year":"1970","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1045","DOI":"10.1016\/0002-9378(73)90552-8","article-title":"Effect of estrogens on the uterine blood flow of oophorectomized ewes","volume":"115","author":"Killam","year":"1973","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1203\/00006450-197303000-00006","article-title":"Effect of estradiol-17, on the magnitude and distribution of uterine blood flow in nonpregnant, oophorectomized ewes","volume":"7","author":"Rosenfeld","year":"1973","journal-title":"Pediatr. Res."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1192","DOI":"10.1210\/endo-94-4-1192","article-title":"The stimulation of uterine blood flow by various estrogens","volume":"94","author":"Resnik","year":"1974","journal-title":"Endocrinology"},{"key":"ref_42","first-page":"E690","article-title":"Systemic and uterine responses to chronic infusion of estradiol-17 beta","volume":"265","author":"Magness","year":"1993","journal-title":"Am. J. Physiol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1124\/pr.107.08002","article-title":"Vascular actions of estrogens: Functional implications","volume":"60","author":"Miller","year":"2008","journal-title":"Pharmacol. Rev."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1161\/HYPERTENSIONAHA.111.201624","article-title":"Aberrant synthesis, metabolism, and plasma accumulation of circulating estrogens and estrogen metabolites in preeclampsia implications for vascular dysfunction","volume":"61","author":"Jobe","year":"2013","journal-title":"Hypertension"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1117","DOI":"10.1038\/nature06951","article-title":"Deficiency in catechol-O-methyltransferase and 2-methoxyoestradiol is associated with pre-eclampsia","volume":"453","author":"Kanasaki","year":"2008","journal-title":"Nature"},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Chambliss, K., Mineo, C., and Shaul, P.W. (2023). Endothelial biology of estrogen and cardiovascular disease. Endocrinology, bqad122.","DOI":"10.1210\/endocr\/bqad122"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"735","DOI":"10.1016\/0002-9378(84)90557-X","article-title":"Uterine vascular effects of estetrol in nonpregnant ewes","volume":"148","author":"Levine","year":"1984","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_48","first-page":"12","article-title":"Premarin-induced increases in coronary and uterine blood flow in nonpregnant sheep","volume":"183","author":"Clark","year":"2000","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1333","DOI":"10.1210\/endo-110-4-1333","article-title":"Induction and inhibition of uterine vasodilation by catechol estrogen in oophorectomized, nonpregnant ewes","volume":"110","author":"Rosenfeld","year":"1982","journal-title":"Endocrinology"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/0090-6980(90)90096-E","article-title":"Uteroplacental production of eicosanoids in ovine pregnancy","volume":"39","author":"Magness","year":"1990","journal-title":"Prostaglandins"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1886","DOI":"10.1095\/biolreprod.103.019901","article-title":"Development and use of an ovarian synchronization model to study the effects of endogenous estrogen and nitric oxide on uterine blood flow during ovarian cycles in sheep","volume":"70","author":"Gibson","year":"2004","journal-title":"Biol. Reprod."},{"key":"ref_52","first-page":"32","article-title":"Control of uterine and ovarian blood flow throughout the estrous cycle and pregnancy of ewes, sows and cows","volume":"55","author":"Ford","year":"1982","journal-title":"J. Anim. Sci."},{"key":"ref_53","first-page":"E464","article-title":"Protein kinase C in uterine and systemic arteries during ovarian cycle and pregnancy","volume":"260","author":"Magness","year":"1991","journal-title":"Am. J. Physiol."},{"key":"ref_54","first-page":"620","article-title":"Uterine artery hemodynamic adaptations through the menstrual cycle into early pregnancy","volume":"99","author":"Bernstein","year":"2002","journal-title":"Obstet. Gynecol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1016\/0002-9378(77)90617-2","article-title":"The effect of progesterone on estrogen-induced uterine blood flow","volume":"128","author":"Resnik","year":"1977","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_56","first-page":"239","article-title":"The role of steroid hormones in the control of uterine blood flow","volume":"Volume 10","author":"Magness","year":"1989","journal-title":"The Uterine Circulation"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"530","DOI":"10.1095\/biolreprod.104.035949","article-title":"Expression of estrogen receptors-alpha and -beta in the pregnant ovine uterine artery endothelial cells in vivo and in vitro","volume":"72","author":"Liao","year":"2005","journal-title":"Biol. Reprod."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1113\/jphysiol.2005.085753","article-title":"Endothelial vasodilator production by ovine uterine and systemic arteries: Ovarian steroid and pregnancy control of ERalpha and ERbeta levels","volume":"565","author":"Byers","year":"2005","journal-title":"J. Physiol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"967","DOI":"10.1161\/HYPERTENSIONAHA.119.13429","article-title":"Estrogen receptor-beta mediates estradiol-induced pregnancy-specific uterine artery endothelial cell angiotensin type-2 receptor expression","volume":"74","author":"Mishra","year":"2019","journal-title":"Hypertension"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"1552","DOI":"10.1093\/jnci\/90.20.1552","article-title":"Effects of the antiestrogens tamoxifen, toremifene, and ICI 182,780 on endometrial cancer growth","volume":"90","author":"Cisneros","year":"1998","journal-title":"J. Natl. Cancer Inst."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1093\/humupd\/6.3.212","article-title":"A pharmacological review of selective oestrogen receptor modulators","volume":"6","author":"Goldstein","year":"2000","journal-title":"Hum. Reprod. Update"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/S0378-4320(98)00068-2","article-title":"Oestrogenic effects of ICI 182,780, a putative anti-oestrogen, on the secretion of oxytocin and prostaglandin F2alpha during oestrous cycle in the intact ewe","volume":"51","author":"Fairclough","year":"1998","journal-title":"Anim. Reprod. Sci."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"544","DOI":"10.1067\/mob.2001.111098","article-title":"Hemodynamic response to tibolone in reproductive and nonreproductive tissues in the sheep","volume":"184","author":"Zoma","year":"2001","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1161\/01.RES.81.3.355","article-title":"Estrogen upregulates endothelial nitric oxide synthase gene expression in fetal pulmonary artery endothelium","volume":"81","author":"MacRitchie","year":"1997","journal-title":"Circ. Res."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"1052","DOI":"10.1161\/HYPERTENSIONAHA.111.189498","article-title":"Endothelial caveolar hub regulation of adenosine triphosphate-induced endothelial nitric oxide synthase subcellular partitioning and domain-specific phosphorylation","volume":"59","author":"Ramadoss","year":"2012","journal-title":"Hypertension"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1172\/JCI5347","article-title":"Estrogen receptor alpha mediates the nongenomic activation of endothelial nitric oxide synthase by estrogen","volume":"103","author":"Chen","year":"1999","journal-title":"J. Clin. Investig."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1038\/21218","article-title":"Regulation of endothelium-derived nitric oxide production by the protein kinase Akt","volume":"399","author":"Fulton","year":"1999","journal-title":"Nature"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"1061","DOI":"10.1089\/ars.2009.2938","article-title":"Hydrogen sulfide: The third gasotransmitter in biology and medicine","volume":"12","author":"Wang","year":"2010","journal-title":"Antioxid. Redox Signal."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"5212","DOI":"10.1073\/pnas.91.11.5212","article-title":"Induction of calcium-dependent nitric oxide synthases by sex hormones","volume":"91","author":"Weiner","year":"1994","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1161\/HYPERTENSIONAHA.111.187559","article-title":"Endothelial nitric oxide synthase deficiency reduces uterine blood flow, spiral artery elongation, and placental oxygenation in pregnant mice","volume":"60","author":"Kulandavelu","year":"2012","journal-title":"Hypertension"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"4349","DOI":"10.3390\/ijms21124349","article-title":"Estrogen receptors and estrogen-induced uterine vasodilation in pregnancy","volume":"21","author":"Bai","year":"2020","journal-title":"Int. J. Mol. Sci."},{"key":"ref_72","doi-asserted-by":"crossref","unstructured":"Tropea, T., De Francesco, E.M., Rigiracciolo, D., Maggiolini, M., Wareing, M., Osol, G., and Mandala, M. (2015). Pregnancy augments G-protein estrogen receptor (GPER) induced vasodilation in rat uterine arteries via the nitric oxide-cGMP signaling pathway. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0141997"},{"key":"ref_73","doi-asserted-by":"crossref","unstructured":"Tropea, T., Rigiracciolo, D., Esposito, M., Maggiolini, M., and Mandala, M. (2022). G-protein-coupled estrogen receptor expression in rat uterine artery is increased by pregnancy and induces dilation in a Ca2+ and ERK1\/2 dependent manner. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23115996"},{"key":"ref_74","doi-asserted-by":"crossref","unstructured":"Lin, A.H., Li, R.W., Ho, E.Y., Leung, G.P., Leung, S.W., Vanhoutte, P.M., and Man, R.Y. (2013). Differential ligand binding affinities of human estrogen receptor-alpha isoforms. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0063199"},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"2399","DOI":"10.1210\/endo.143.6.8873","article-title":"ER function in the adult male rat: Short- and long-term effects of the antiestrogen ICI 182,780 on the testis and efferent ductules, without changes in testosterone","volume":"143","author":"Oliveira","year":"2002","journal-title":"Endocrinology"},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"1902","DOI":"10.1161\/HYPERTENSIONAHA.121.18190","article-title":"Enhanced Sp1\/YY1 expression directs CBS transcription to mediate VEGF-stimulated pregnancy-dependent H2S production in human uterine artery endothelial cells","volume":"78","author":"Bai","year":"2021","journal-title":"Hypertension"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/18\/14384\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T20:54:45Z","timestamp":1760129685000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/24\/18\/14384"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,9,21]]},"references-count":76,"journal-issue":{"issue":"18","published-online":{"date-parts":[[2023,9]]}},"alternative-id":["ijms241814384"],"URL":"https:\/\/doi.org\/10.3390\/ijms241814384","relation":{},"ISSN":["1422-0067"],"issn-type":[{"type":"electronic","value":"1422-0067"}],"subject":[],"published":{"date-parts":[[2023,9,21]]}}}