{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T02:30:41Z","timestamp":1760149841393,"version":"build-2065373602"},"reference-count":82,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2023,9,23]],"date-time":"2023-09-23T00:00:00Z","timestamp":1695427200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>One hundred and twenty-five years ago there was a lively discussion between Hungarian and Spanish neuroscientists on the nature of neural connections. The question was whether the neurofibrils run from one neuron to the next and connect neurons as a continuous network or the fibrils form an internal skeleton in the neurons and do not leave the cell; however, there is close contact between the neurons. About 50 years later, the invention of the electron microscope solved the problem. Close contacts between individual neurons were identified and named as synapses. In the following years, the need arose to explore distant connections between neuronal structures. Tracing techniques entered neuroscience. There are three major groups of tracers: (A) non-transsynaptic tracers used to find direct connections between two neuronal structures; (B) tracers passing gap junctions; (C) transsynaptic tracers passing synapses that are suitable to explore multineuronal circuits. According to the direction of the transport mechanism, the tracer may be ante- or retrograde. In this review, we focus on the ever-increasing number of fluorescent tracers that we have also used in our studies. The advantage of the use of these molecules is that the fluorescence of the tracer can be seen in histological sections without any other processes. Genes encoding fluorescent molecules can be inserted in various neuropeptide or neurotransmitter expressing transcriptomes. This makes it possible to study the anatomy, development or functional relations of these neuronal networks in transgenic animals.<\/jats:p>","DOI":"10.3390\/ijms241914478","type":"journal-article","created":{"date-parts":[[2023,9,24]],"date-time":"2023-09-24T10:36:26Z","timestamp":1695551786000},"page":"14478","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Fluorescent Molecules That Help Reveal Previously Unidentified Neural Connections in Adult, Neonatal and Peripubertal Mammals"],"prefix":"10.3390","volume":"24","author":[{"given":"Enik\u0151","family":"Vaszin\u00e9 Szab\u00f3","sequence":"first","affiliation":[{"name":"Department of Restorative Dentistry and Endodontics, Faculty of Dentistry, Semmelweis University, Szentkir\u00e1lyi u. 47, H-1088 Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katalin","family":"K\u00f6ves","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Histology and Embryology, Faculty of Medicine, Semmelweis University, T\u0171zolt\u00f3 u. 58, H-1094 Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u00c1gnes","family":"Cs\u00e1ki","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Histology and Embryology, Faculty of Medicine, Semmelweis University, T\u0171zolt\u00f3 u. 58, H-1094 Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,9,23]]},"reference":[{"key":"ref_1","unstructured":"Ap\u00e1thy, S. (1888). The Leading Element of the Nervous System and Its Topographical Reviews on the Cells. Messages from the Zoological Station at Naples, Stazione Zoologica Anton Dohrn."},{"key":"ref_2","first-page":"100","article-title":"A new proof of the function of neurofibrils","volume":"1","author":"Bethe","year":"1907","journal-title":"Folia Neurobiol."},{"key":"ref_3","unstructured":"Ramon y Cajal, S. (1967). Nobel Lecture. Neurons and Synapses: The History of Its Discovery, Elsevier Publishing Co.. 1906, Nobel Lectures, Physiology or Medicine 1901\u20131921."},{"key":"ref_4","first-page":"389","article-title":"Die magnetische Sammelspule f\u00fcr schnelle Elektronenstrahlen. (The magnetic concentrating coil for fast electron beams.)","volume":"12","author":"Ruska","year":"1931","journal-title":"Z. techn. Physik"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1083\/jcb.2.5.531","article-title":"Electron microscopic observations of the central nervous system","volume":"2","author":"Luse","year":"1956","journal-title":"J. Biophysic. Biochemic. Cytol."},{"key":"ref_6","first-page":"299","article-title":"The Ferrier Lecture: On problems connected with the principle of humoral transmission of nervous impulses","volume":"118B","author":"Loewi","year":"1935","journal-title":"Proc. R. Soc."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1113\/jphysiol.1952.sp004799","article-title":"The electrical constants of Purkinje fibres","volume":"118","author":"Weidmann","year":"1952","journal-title":"J. Physiol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"2019","DOI":"10.3389\/fnins.2019.00897","article-title":"A Student\u2019s Guide to Neural Circuit Tracing","volume":"13","author":"Saleeba","year":"2019","journal-title":"Front. Neurosci."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1016\/0006-8993(68)90052-8","article-title":"Evaluation of a radioautographic neuroanatomical tracing method","volume":"8","author":"Lasek","year":"1968","journal-title":"Brain Res."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1002\/cne.903260110","article-title":"Enkephalin-immunoreactive neurons in the parvicellular subdivisions of the paraventricular nucleus project to the external zone of the median eminence","volume":"326","author":"Merchenthaler","year":"1992","journal-title":"J. Comp. Neurol."},{"key":"ref_11","first-page":"SR5","article-title":"PACAP and VIP in the photoneuroendocrine system (PNES)","volume":"8","author":"Vereczki","year":"2002","journal-title":"Med. Sci. Mon."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"8629","DOI":"10.1523\/JNEUROSCI.20-22-08629.2000","article-title":"A series of biotinylated tracers distinguishes three types of gap junction in retina","volume":"20","author":"Mills","year":"2000","journal-title":"J. Neurosci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"367","DOI":"10.3109\/15419060009109019","article-title":"Quantitative determination of Ggp junction intercellular communication by scrape loading and image analysis","volume":"7","author":"Opsahl","year":"2000","journal-title":"Cell Adhes. Commun."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1566","DOI":"10.1021\/acsbiomaterials.1c01459","article-title":"Gap junction-mediated delivery of polymeric macromolecules","volume":"8","author":"Trementozzi","year":"2022","journal-title":"ACS Biomater. Sci. Eng."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1016\/j.jneumeth.2009.12.001","article-title":"Advances in viral transneuronal tracing","volume":"194","author":"Ugolini","year":"2010","journal-title":"J. Neurosci. Methods"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1007\/BF00686894","article-title":"Transport of fluorescent protein tracer in peripheral nerves","volume":"16","author":"Kristensson","year":"1970","journal-title":"Acta Neuropath."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1016\/B978-0-12-008305-3.50014-3","article-title":"Fluorescent Neuronal Tracers","volume":"Volume 5","author":"Fedoroff","year":"1984","journal-title":"Advances in Cellular Neurobiology"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1126\/science.39.1004.443","article-title":"The action of vital stains belonging to the benzidine group","volume":"39","author":"Evans","year":"1914","journal-title":"Science"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"7628037","DOI":"10.1155\/2018\/7628037","article-title":"Evans Blue Dye: A Revisit of Its Applications in Biomedicine","volume":"2018","author":"Yao","year":"2018","journal-title":"Contrast Media Mol. Imaging"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"542","DOI":"10.1097\/00005072-196610000-00004","article-title":"Selective vulnerability of the blood-brain barrier in chemically induced lesions","volume":"25","author":"Steinwall","year":"1966","journal-title":"J. Neuropathol. Exper. Neurol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/0304-3940(76)90072-0","article-title":"Retrograde labeling of central nervous pathways with tritiated or Evans blue-labeled bovine serum albumin","volume":"3","author":"Steward","year":"1976","journal-title":"Neurosci. Lett."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/0006-8993(78)90014-8","article-title":"Single mammillary body cells with divergent axon collaterals. Demonstration by a simple fluorescent retrograde double labeling technique in the rat","volume":"158","author":"Kuypers","year":"1978","journal-title":"Brain Res."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/0304-3940(80)90208-6","article-title":"Two new fluorescent retrograde neuronal tracers which are transported over long distances","volume":"18","author":"Bentivoglio","year":"1980","journal-title":"Neurosci. Lett."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1007\/BF00236147","article-title":"Double retrograde neuronal labeling through divergent axon collaterals, using two fluorescent tracers with the same excitation wavelength which label different features of the cell","volume":"40","author":"Kuypers","year":"1980","journal-title":"Exp. Brain Res."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1007\/BF00237193","article-title":"Diamidino yellow dihydrochloride (DY.2HCl); a new fluorescent retrograde neuronal tracer, which migrates only very slowly out of the cell","volume":"51","author":"Keizer","year":"1983","journal-title":"Exp. Brain Res."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/0006-8993(82)90177-9","article-title":"SITS: A covalently bound fluorescent retrograde tracer that does not appear to be taken up by fibers-of-passage","volume":"249","author":"Schmued","year":"1982","journal-title":"Brain Res."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/0006-8993(86)91199-6","article-title":"Fluoro-gold: A new fluorescent retrograde axonal tracer with numerous unique properties","volume":"377","author":"Schmued","year":"1986","journal-title":"Brain Res."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"498","DOI":"10.1038\/310498a0","article-title":"Fluorescent latex microspheres as a retrograde neuronal marker for in vivo and in vitro studies of visual cortex","volume":"310","author":"Katz","year":"1984","journal-title":"Nature"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"909","DOI":"10.1007\/s12264-019-00374-9","article-title":"Trans-synaptic Neural Circuit-Tracing with Neurotropic Viruses","volume":"35","author":"Li","year":"2019","journal-title":"Neurosci. Bull."},{"key":"ref_30","first-page":"42","article-title":"Experimental reduction of virulence of Aujeszky\u2019s disease virus","volume":"16","author":"Bartha","year":"1961","journal-title":"Magy. \u00c1llatorv. Lapja"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"462","DOI":"10.1128\/MMBR.69.3.462-500.2005","article-title":"Molecular biology of pseudorabies virus: Impact on neurovirology and veterinary medicine","volume":"9","author":"Pomeranz","year":"2005","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"4030","DOI":"10.1128\/jvi.61.12.4030-4032.1987","article-title":"Role of a structural glycoprotein of pseudorabies in virus virulence","volume":"61","author":"Mettenleiter","year":"1987","journal-title":"J. Virol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"9431","DOI":"10.1128\/JVI.01317-13","article-title":"Glycoproteins gE and gI are required for efficient KIF1A-dependent anterograde axonal transport of alphaherpesvirus particles in neurons","volume":"87","author":"Kratchmarov","year":"2013","journal-title":"J. Virol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/S0169-328X(02)00546-6","article-title":"Construction of recombinant pseudorabies viruses optimized for labeling and neurochemical characterization of neural circuitry","volume":"109","author":"Reichart","year":"2002","journal-title":"Brain Res. Mol. Brain Res."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1016\/0042-6822(88)90079-7","article-title":"Beta-galactosidase as a marker in the peripheral and neural tissues of the herpes simplex virus-infected mouse","volume":"167","author":"Ho","year":"1988","journal-title":"Virology"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"15414","DOI":"10.1073\/pnas.1110854108","article-title":"Anterograde or retrograde transsynaptic labeling of CNS neurons with vesicular stomatitis virus vectors","volume":"108","author":"Beier","year":"2011","journal-title":"PNAS"},{"key":"ref_37","first-page":"10073","article-title":"GFP: Lighting Up Life","volume":"106","author":"Shimomura","year":"2008","journal-title":"Nobel Lect."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"3991","DOI":"10.1021\/bi00838a015","article-title":"Properties of the bioluminescent protein aequorin","volume":"8","author":"Shimomura","year":"1969","journal-title":"Biochemistry"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"802","DOI":"10.1126\/science.8303295","article-title":"Green fluorescent protein as a marker for gene expression","volume":"263","author":"Chalfie","year":"1994","journal-title":"Science"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/j.cell.2004.09.012","article-title":"Combinatorial marking of cells and organelles with reconstituted fluorescent proteins","volume":"119","author":"Zhang","year":"2004","journal-title":"Cell"},{"key":"ref_41","first-page":"151","article-title":"Red-shifted excitation mutants of the green fluorescent protein","volume":"13","author":"Delagrave","year":"1995","journal-title":"Biotechnology"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1146\/annurev.biochem.67.1.509","article-title":"The green fluorescent protein","volume":"67","author":"Tsien","year":"1998","journal-title":"Annu. Rev. Biochem."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1002\/jemt.20370","article-title":"Sensitivity of CFP\/YFP and GFP\/mCherry pairs to donor photobleaching on FRET determination by fluorescence lifetime imaging microscopy in living cells","volume":"69","author":"Tramier","year":"2006","journal-title":"Microsc. Res. Tech."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"969","DOI":"10.1038\/13657","article-title":"Fluorescent proteins from nonbioluminescent Anthozoa species","volume":"17","author":"Matz","year":"1999","journal-title":"Nat. Biotechnol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1038\/nmeth1062","article-title":"Bright monomeric red fluorescent protein with an extended fluorescence lifetime","volume":"4","author":"Merzlyak","year":"2007","journal-title":"Nat. Methods"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1016\/S0166-0934(97)00065-7","article-title":"Green fluorescent protein expressed by recombinant pseudorabies virus as an in vivo marker for viral replication","volume":"66","author":"Mettenleiter","year":"1997","journal-title":"J. Virol. Method."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1038\/nmeth.1292","article-title":"Genetically timed, activity-sensor and rainbow transsynaptic viral tools","volume":"6","author":"Awatramani","year":"2009","journal-title":"Nat. Methods"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"10640","DOI":"10.1523\/JNEUROSCI.18-24-10640.1998","article-title":"Selective neuronal expression of green fluorescent protein with cytomegalovirus promoter reveals entire neuronal arbor in transgenic mice","volume":"18","author":"Ghosh","year":"1998","journal-title":"J. Neurosci."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1212","DOI":"10.1016\/j.biopsych.2010.01.026","article-title":"A novel transgenic mouse for gene-targeting within cells that express corticotropin-releasing factor","volume":"67","author":"Martin","year":"2010","journal-title":"Biol. Psychiatry"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1111\/jne.12442","article-title":"Targeting corticotropin-releasing factor (CRF) projections from the oval nucleus of the BNST using cell-type specific neuronal tracing studies in mouse and rat brain","volume":"28","author":"Dabrowska","year":"2016","journal-title":"J. Neuroendocrinol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"4854","DOI":"10.1093\/cercor\/bhv202","article-title":"Characterizing VIP Neurons in the Barrel Cortex of VIPcre\/tdTomato Mice Reveals Layer-Specific Differences","volume":"25","author":"Scheuer","year":"2015","journal-title":"Cerebral Cortex"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"3354","DOI":"10.1523\/JNEUROSCI.20-09-03354.2000","article-title":"Novel hippocampal interneuronal subtypes identified using transgenic mice that express green fluorescent protein in GABAergic interneurons","volume":"20","author":"Oliva","year":"2000","journal-title":"J. Neurosci."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1002\/cne.22229","article-title":"Immunochemical characterization of inhibitory mouse cortical neurons: Three chemically distinct classes of inhibitory cells","volume":"518","author":"Xu","year":"2010","journal-title":"J. Comp. Neurol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"10251","DOI":"10.1523\/JNEUROSCI.22-23-10251.2002","article-title":"Number, Density, and Surface\/Cytoplasmic Distribution of GABA Transporters at Presynaptic Structures of Knock-In Mice Carrying GABA Transporter Subtype 1-Green Fluorescent Protein Fusions","volume":"22","author":"Chiu","year":"2002","journal-title":"J. Neurosci."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/j.brainres.2010.05.061","article-title":"Mapping of fluorescent protein-expressing neurons and axon pathways in adult and developing Thy1-eYFP-H transgenic mice","volume":"1345","author":"Porrero","year":"2010","journal-title":"Brain Res."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/S0896-6273(00)00084-2","article-title":"Imaging neuronal subsets in transgenic mice expressing multiple spectral variants of GFP","volume":"28","author":"Feng","year":"2000","journal-title":"Neuron"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"2037","DOI":"10.1523\/JNEUROSCI.19-06-02037.1999","article-title":"GABA- and glutamate-activated channels in green fluorescent protein-tagged gonadotropin-releasing hormone neurons in transgenic mice","volume":"19","author":"Spergel","year":"1999","journal-title":"J. Neurosci."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"7143","DOI":"10.1523\/JNEUROSCI.23-18-07143.2003","article-title":"Transgenic mice expressing green fluorescent protein under the control of the melanocortin-4 receptor promoter","volume":"23","author":"Liu","year":"2003","journal-title":"J. Neurosci."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"1991","DOI":"10.1097\/00001756-200006260-00037","article-title":"Visualization of neurogenesis in the central nervous system using nestin promoter-GFP transgenic mice","volume":"11","author":"Yamaguchi","year":"2000","journal-title":"Neuroreport"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"10120","DOI":"10.1073\/pnas.152266799","article-title":"Retrograde trans-synaptic transfer of green fluorescent protein allows the genetic mapping of neuronal circuits in transgenic mice","volume":"99","author":"Maskos","year":"2002","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1152\/physiolgenomics.00092.2006","article-title":"BAC transgenic mice express enhanced green fluorescent protein in central and peripheral cholinergic neurons","volume":"27","author":"Tallini","year":"2006","journal-title":"Physiol. Genom."},{"key":"ref_62","doi-asserted-by":"crossref","unstructured":"Llewellyn-Smith, I.J., and Verberne, A.J.M. (2011). Central Regulation of Autonomic Functions, Oxford University Press. [2nd ed.]. Chapter 1.","DOI":"10.1093\/acprof:oso\/9780195306637.001.0001"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/j.autneu.2015.03.005","article-title":"Identification of autonomic neuronal chains innervating gingiva and lip","volume":"190","year":"2015","journal-title":"Auton. Neurosci."},{"key":"ref_64","first-page":"7","article-title":"Hypothalamic neuropeptides in the autonomic innervation of gingiva and lip","volume":"1","author":"Lohinai","year":"2016","journal-title":"J. Trans. Neurosci."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1556\/APhysiol.99.2012.2.8","article-title":"Characterization of the autonomic innervation of mammary gland in lactating rats studied by retrograde transynaptic virus labeling and immunohistochemistry","volume":"99","year":"2012","journal-title":"Acta Physiol. Hung."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1111\/j.1600-079X.1991.tb00005.x","article-title":"GABA-immunoreactive intrinsic and immunonegative secondary neurons in the cat pineal organ","volume":"10","author":"Petter","year":"1991","journal-title":"J. Pineal Res."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1002\/1096-9861(20010129)430:1<72::AID-CNE1015>3.0.CO;2-T","article-title":"Central GABAergic innervation of the mammalian pineal gland: A light and electron microscopic immunocytochemical investigation in rodent and nonrodent species","volume":"430","author":"Sakai","year":"2001","journal-title":"J. Comp. Neurol."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1679\/aohc.57.47","article-title":"Immunohistochemical studies on sympathetic and non-sympathetic nerve fibers and neuronal cell bodies in the pineal gland of cotton rats, Sigmodon hispidus","volume":"57","author":"Matsushima","year":"1994","journal-title":"Arch. Histol. Cytol."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1007\/BF00212535","article-title":"Fine-structural study of the pinealbody of 3 monkey (Macaca fuscata) with special reference to synapticformations","volume":"244","author":"Ichimura","year":"1986","journal-title":"Cell Tissue Res."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/cne.901460102","article-title":"A retinohypothalamic projection in the rat","volume":"146","author":"Moore","year":"1972","journal-title":"J. Comp. Neurol."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1002\/(SICI)1096-9861(19990405)406:2<171::AID-CNE3>3.0.CO;2-U","article-title":"Anatomical demonstration of the suprachiasmatic nucleus-pineal pathway","volume":"406","author":"Postema","year":"1999","journal-title":"J. Comp. Neurol."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.neulet.2014.12.043","article-title":"Is a neuronal chain between the pineal body and the retina in rats and hamsters? Transneural tracing studies","volume":"588","author":"Vereczki","year":"2015","journal-title":"Neurosci. Lett."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/j.neulet.2017.12.007","article-title":"Ontogenesis of the pinealo-retinal neuronal connection in albino rats","volume":"665","author":"Vereczki","year":"2018","journal-title":"Neurosci. Lett."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"135517","DOI":"10.1016\/j.neulet.2020.135517","article-title":"Pinealocytes cannot transport neurotropic viruses. Pinealo-to-retinal connection in prepubertal rats originates from pineal neurons: Light and electron microscopic immunohistochemical studies","volume":"744","author":"Kiss","year":"2021","journal-title":"Neurosci. Lett."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"661","DOI":"10.1006\/bbrc.2000.3350","article-title":"A novel avian hypothalamic peptide inhibiting gonadotropin release","volume":"275","author":"Tsutsui","year":"2000","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1016\/j.ygcen.2012.02.013","article-title":"Gonadotropin-inhibitory hormone (GnIH): Discovery, progress and prospect","volume":"177","author":"Tsutsui","year":"2012","journal-title":"Gen. Comp. Endocrinol."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"27","DOI":"10.3390\/neurosci2010002","article-title":"The same magnocellular neurons send axon collaterals to the posterior pituitary and retina or to the posterior pituitary and autonomic preganglionic centers of the eye in rats","volume":"2","year":"2021","journal-title":"NeuroSci"},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/0006-8993(84)90940-5","article-title":"An anterograde neuroanatomical tracing method that shows the detailed morphology of neurons, their axons and terminals: Immunohistochemical localization of an axonally transported plant lectin, Phaseolus vulgaris leucoagglutinin (PHA-L)","volume":"290","author":"Gerfen","year":"1984","journal-title":"Brain Res."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"1157","DOI":"10.1038\/nprot.2009.93","article-title":"Multiple neuroanatomical tract-tracing using fluorescent Alexa Fluor conjugates of cholera toxin subunit B in rats","volume":"4","author":"Conte","year":"2009","journal-title":"Nat. Protoc."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/0304-3940(91)90024-N","article-title":"Many diverse types of retinal neurons show tracer coupling when injected with biocytin or Neurobiotin","volume":"125","author":"Vaney","year":"1991","journal-title":"J. Neurosci. Lett."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/0165-0270(92)90121-S","article-title":"Neurobiotin, a useful neuroanatomical tracer for in vivo anterograde, retrograde and transneuronal tract-tracing and for in vitro labeling of neurons","volume":"41","author":"Huang","year":"1992","journal-title":"J. Neurosci. Methods"},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"765","DOI":"10.1017\/S0952523898154159","article-title":"The kinetics of tracer movement through homologous gap junctions in the rabbit retina","volume":"15","author":"Mills","year":"1998","journal-title":"Vis. 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