{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T07:25:15Z","timestamp":1775633115809,"version":"3.50.1"},"reference-count":24,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2023,9,25]],"date-time":"2023-09-25T00:00:00Z","timestamp":1695600000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"AGENZIA NAZIONALE DI VALUTAZIONE DEL SISTEMA UNIVERSITARIO E DELLA RICERCA (ANVUR)","award":["n. 232 c.295"],"award-info":[{"award-number":["n. 232 c.295"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>This study aimed to examine the impact of different surface properties of poly(lactic-co-glycolic) acid (PLGA) nanoparticles (P NPs) and PLGA-Poloxamer nanoparticles (PP NPs) on their in vivo biodistribution. For this purpose, NPs were formulated via nanoprecipitation and loaded with diphenylhexatriene (DPH), a fluorescent dye. The obtained NPs underwent comprehensive characterization, encompassing their morphology, technological attributes, DPH release rate, and thermodynamic properties. The produced NPs were then administered to wild-type mice via intraperitoneal injection, and, at scheduled time intervals, the animals were euthanized. Blood samples, as well as the liver, lungs, and kidneys, were extracted for histological examination and biodistribution analysis. The findings of this investigation revealed that the presence of poloxamers led to smaller NP sizes and induced partial crystallinity in the NPs. The biodistribution and histological results from in vivo experiments evidenced that both, P and PP NPs, exhibited comparable concentrations in the bloodstream, while P NPs could not be detected in the other organs examined. Conversely, PP NPs were primarily sequestered by the lungs and, to a lesser extent, by the kidneys. Future research endeavors will focus on investigating the behavior of drug-loaded NPs in pathological animal models.<\/jats:p>","DOI":"10.3390\/ijms241914523","type":"journal-article","created":{"date-parts":[[2023,9,26]],"date-time":"2023-09-26T03:53:00Z","timestamp":1695700380000},"page":"14523","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Investigating the Effect of Surface Hydrophilicity on the Destiny of PLGA-Poloxamer Nanoparticles in an In Vivo Animal Model"],"prefix":"10.3390","volume":"24","author":[{"given":"Teresa","family":"Silvestri","sequence":"first","affiliation":[{"name":"Department of Pharmacy-Pharmaceutical Sciences, University of Bari Aldo Moro, Via E. Orabona 4, 70125 Bari, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3529-1819","authenticated-orcid":false,"given":"Lucia","family":"Grumetto","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, University of Naples Federico II, Via D. Montesano 49, 80131 Naples, Italy"},{"name":"National Institute of Biostructures and Biosystems (INBB), Viale delle Medaglie d\u2019Oro 305, 00136 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2075-104X","authenticated-orcid":false,"given":"Ilaria","family":"Neri","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, University of Naples Federico II, Via D. Montesano 49, 80131 Naples, Italy"},{"name":"National Institute of Biostructures and Biosystems (INBB), Viale delle Medaglie d\u2019Oro 305, 00136 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4617-8360","authenticated-orcid":false,"given":"Maria","family":"De Falco","sequence":"additional","affiliation":[{"name":"National Institute of Biostructures and Biosystems (INBB), Viale delle Medaglie d\u2019Oro 305, 00136 Rome, Italy"},{"name":"Department of Biology, University Federico II of Naples, Via Cinthia 26, 80125 Naples, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9512-6217","authenticated-orcid":false,"given":"Sossio Fabio","family":"Graziano","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, University of Naples Federico II, Via D. Montesano 49, 80131 Naples, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sara","family":"Damiano","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, Via Federico Delpino 1, 80137 Naples, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniela","family":"Giaquinto","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, Via Federico Delpino 1, 80137 Naples, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2697-9339","authenticated-orcid":false,"given":"Lucianna","family":"Maruccio","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, Via Federico Delpino 1, 80137 Naples, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paolo","family":"de Girolamo","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, Via Federico Delpino 1, 80137 Naples, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabrizio","family":"Villapiano","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, University of Naples Federico II, Via D. Montesano 49, 80131 Naples, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4121-4905","authenticated-orcid":false,"given":"Roberto","family":"Ciarcia","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, Via Federico Delpino 1, 80137 Naples, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8869-6465","authenticated-orcid":false,"given":"Laura","family":"Mayol","sequence":"additional","affiliation":[{"name":"Department of Advanced Biomedical Sciences, University of Naples Federico II, Via D. Montesano 49, 80131 Naples, Italy"},{"name":"Interdisciplinary Research Centre on Biomaterials (CRIB), Piazzale Tecchio 80, 80125 Naples, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marco","family":"Biondi","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, University of Naples Federico II, Via D. Montesano 49, 80131 Naples, Italy"},{"name":"Interdisciplinary Research Centre on Biomaterials (CRIB), Piazzale Tecchio 80, 80125 Naples, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,9,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"611","DOI":"10.3892\/or.2017.5718","article-title":"Cancer Drug Delivery in the Nano Era: An Overview and Perspectives (Review)","volume":"38","author":"Li","year":"2017","journal-title":"Oncol. 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