{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T23:23:07Z","timestamp":1768778587857,"version":"3.49.0"},"reference-count":38,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2023,9,26]],"date-time":"2023-09-26T00:00:00Z","timestamp":1695686400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003725","name":"Basic Science Research Program of the National Research Foundation of Korea funded by the Ministry of Science and ICT","doi-asserted-by":"publisher","award":["RS-2023-00209591"],"award-info":[{"award-number":["RS-2023-00209591"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Chungbuk National University Hospital","award":["RS-2023-00209591"],"award-info":[{"award-number":["RS-2023-00209591"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The purpose of this study was to investigate the anti-inflammatory effect of tegoprazan (TEGO) in lipopolysaccharide (LPS)-stimulated bone-marrow-derived macrophages (BMMs). To this end, compared to methylprednisolone (MP; positive control), we evaluated whether TEGO effectively differentiates LPS-stimulated BMMs into M2-phenotype macrophages. Moreover, the expression of pro- and anti-inflammatory cytokines genes influenced by TEGO was measured using quantitative real-time polymerase chain reaction (qRT-PCR) analysis. TEGO was found to reduce nitric oxide (NO) production in BMMs significantly. In addition, TEGO significantly decreased and increased the gene expression levels of pro-inflammatory and anti-inflammatory cytokines, respectively. In addition, we evaluated the phosphorylated values of the extracellular signal-regulatory kinase (ERK) and p38 in the mitogen-activated protein (MAP) kinase signaling pathway through Western blotting. TEGO significantly reduced the phosphorylated values of the ERK and p38. In other words, TEGO suppressed the various pro-inflammatory responses in LPS-induced BMMs. These results show that TEGO has the potential to be used as an anti-inflammatory agent.<\/jats:p>","DOI":"10.3390\/ijms241914589","type":"journal-article","created":{"date-parts":[[2023,9,27]],"date-time":"2023-09-27T03:04:18Z","timestamp":1695783858000},"page":"14589","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Anti-Inflammatory Effects of Tegoprazan in Lipopolysaccharide-Stimulated Bone-Marrow-Derived Macrophages"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-2081-9465","authenticated-orcid":false,"given":"Gong-Ho","family":"Han","sequence":"first","affiliation":[{"name":"Department of Neurosurgery, CHA Bundang Medical Center, CHA University, 59 Yatap-ro, Bundang-gu, Seongnam-si 13496, Gyeonggi-do, Republic of Korea"},{"name":"Department of Life Science, CHA University, Boondagger, Seongnam-si 13493, Gyeonggi-do, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5611-3133","authenticated-orcid":false,"given":"Seong-Jun","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, CHA Bundang Medical Center, CHA University, 59 Yatap-ro, Bundang-gu, Seongnam-si 13496, Gyeonggi-do, Republic of Korea"},{"name":"Department of Life Science, CHA University, Boondagger, Seongnam-si 13493, Gyeonggi-do, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wan-Kyu","family":"Ko","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, CHA Bundang Medical Center, CHA University, 59 Yatap-ro, Bundang-gu, Seongnam-si 13496, Gyeonggi-do, Republic of Korea"},{"name":"Department of Life Science, CHA University, Boondagger, Seongnam-si 13493, Gyeonggi-do, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4812-565X","authenticated-orcid":false,"given":"Je-Beom","family":"Hong","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine, Seoul 16419, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0454-8810","authenticated-orcid":false,"given":"Seung-Hun","family":"Sheen","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, CHA Bundang Medical Center, CHA University, 59 Yatap-ro, Bundang-gu, Seongnam-si 13496, Gyeonggi-do, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min-Jai","family":"Cho","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, Chungbuk National University College of Medicine, Chungbuk National University Hospital, Seowon-gu, Cheongju-si 28644, Chungcheong-do, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5724-8099","authenticated-orcid":false,"given":"Seil","family":"Sohn","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, CHA Bundang Medical Center, CHA University, 59 Yatap-ro, Bundang-gu, Seongnam-si 13496, Gyeonggi-do, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,9,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1162","DOI":"10.1378\/chest.117.4.1162","article-title":"Anti-inflammatory cytokines","volume":"117","author":"Opal","year":"2000","journal-title":"Chest"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"428","DOI":"10.1038\/nature07201","article-title":"Origin and physiological roles of inflammation","volume":"454","author":"Medzhitov","year":"2008","journal-title":"Nature"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1016\/j.tox.2004.11.035","article-title":"Nitric oxide and cell viability in inflammatory cells: A role for NO in macrophage function and fate","volume":"208","author":"Bosca","year":"2005","journal-title":"Toxicology"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1016\/S0955-0674(97)80061-0","article-title":"Mitogen-activated protein kinase pathways","volume":"9","author":"Robinson","year":"1997","journal-title":"Curr. 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