{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T15:01:58Z","timestamp":1781103718299,"version":"3.54.1"},"reference-count":29,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2023,9,27]],"date-time":"2023-09-27T00:00:00Z","timestamp":1695772800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"MEXT\/AMED Translational Research Network Program","award":["J190000613"],"award-info":[{"award-number":["J190000613"]}]},{"name":"MEXT\/AMED Translational Research Network Program","award":["C, 19K09448"],"award-info":[{"award-number":["C, 19K09448"]}]},{"name":"MEXT\/AMED Translational Research Network Program","award":["B, 22H03180"],"award-info":[{"award-number":["B, 22H03180"]}]},{"name":"JSPS Grant-in-Aid for Scientific Research","award":["J190000613"],"award-info":[{"award-number":["J190000613"]}]},{"name":"JSPS Grant-in-Aid for Scientific Research","award":["C, 19K09448"],"award-info":[{"award-number":["C, 19K09448"]}]},{"name":"JSPS Grant-in-Aid for Scientific Research","award":["B, 22H03180"],"award-info":[{"award-number":["B, 22H03180"]}]},{"name":"HIROMI Medical Research Foundation","award":["J190000613"],"award-info":[{"award-number":["J190000613"]}]},{"name":"HIROMI Medical Research Foundation","award":["C, 19K09448"],"award-info":[{"award-number":["C, 19K09448"]}]},{"name":"HIROMI Medical Research Foundation","award":["B, 22H03180"],"award-info":[{"award-number":["B, 22H03180"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Multilineage-differentiating stress-enduring (Muse) cells are newly established pluripotent stem cells. The aim of the present study was to examine the potential of the systemic administration of Muse cells as an effective treatment for subacute SCI. We intravenously administered the clinical product \u201cCL2020\u201d containing Muse cells to a rat model two weeks after mid-thoracic spinal cord contusion. Eight experimental animals received CL2020, and twelve received the vehicle. Behavioral analyses were conducted over 20 weeks. Histological evaluations were performed. After 20 weeks of observation, diphtheria toxin was administered to three CL2020-treated animals to selectively ablate human cell functions. Hindlimb motor functions significantly improved from 6 to 20 weeks after the administration of CL2020. The cystic cavity was smaller in the CL2020 group. Furthermore, larger numbers of descending 5-HT fibers were preserved in the distal spinal cord. Muse cells in CL2020 were considered to have differentiated into neuronal and neural cells in the injured spinal cord. Neuronal and neural cells were identified in the gray and white matter, respectively. Importantly, these effects were reversed by the selective ablation of human cells by diphtheria toxin. Intravenously administered Muse cells facilitated the therapeutic potential of CL2020 for severe subacute spinal cord injury.<\/jats:p>","DOI":"10.3390\/ijms241914603","type":"journal-article","created":{"date-parts":[[2023,9,27]],"date-time":"2023-09-27T03:04:18Z","timestamp":1695783858000},"page":"14603","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Intravenous Administration of Human Muse Cells Ameliorates Deficits in a Rat Model of Subacute Spinal Cord Injury"],"prefix":"10.3390","volume":"24","author":[{"given":"Yoshiharu","family":"Takahashi","sequence":"first","affiliation":[{"name":"Department of Neurosurgery, Graduate School of Medicine, Tohoku University, Sendai 980-8572, Japan"},{"name":"Department of Neurosurgery, Tohoku Medical and Pharmaceutical University, Sendai 981-8558, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takumi","family":"Kajitani","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, Graduate School of Medicine, Tohoku University, Sendai 980-8572, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5609-200X","authenticated-orcid":false,"given":"Toshiki","family":"Endo","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, Graduate School of Medicine, Tohoku University, Sendai 980-8572, Japan"},{"name":"Department of Neurosurgery, Tohoku Medical and Pharmaceutical University, Sendai 981-8558, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atsushi","family":"Nakayashiki","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, Graduate School of Medicine, Tohoku University, Sendai 980-8572, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomoo","family":"Inoue","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, Saitama Red Cross Hospital, Saitama 330-8553, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9282-6499","authenticated-orcid":false,"given":"Kuniyasu","family":"Niizuma","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, Graduate School of Medicine, Tohoku University, Sendai 980-8572, Japan"},{"name":"Department of Neurosurgical Engineering and Translational Neuroscience, Graduate School of Medicine, Tohoku University, Sendai 980-8576, Japan"},{"name":"Department of Neurosurgical Engineering and Translational Neuroscience, Graduate School of Biomedical Engineering, Tohoku University, Sendai 980-8572, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teiji","family":"Tominaga","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, Graduate School of Medicine, Tohoku University, Sendai 980-8572, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,9,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"703","DOI":"10.1038\/nrn1195","article-title":"Myelin-Associated Inhibitors of Axonal Regeneration in the Adult Mammalian CNS","volume":"4","author":"Filbin","year":"2003","journal-title":"Nat. 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