{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T19:06:21Z","timestamp":1774465581212,"version":"3.50.1"},"reference-count":64,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2023,9,28]],"date-time":"2023-09-28T00:00:00Z","timestamp":1695859200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003627","name":"Rural Development Administration","doi-asserted-by":"publisher","award":["PJ015500"],"award-info":[{"award-number":["PJ015500"]}],"id":[{"id":"10.13039\/501100003627","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Massey University publication","award":["PJ015500"],"award-info":[{"award-number":["PJ015500"]}]},{"name":"Plant &amp; Food Research Pipfruit phenotyping Technology Development","award":["PJ015500"],"award-info":[{"award-number":["PJ015500"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The YopJ group of acetylating effectors from phytopathogens of the genera Pseudomonas and Ralstonia have been widely studied to understand how they modify and suppress their host defence targets. In contrast, studies on a related group of effectors, the Eop1 group, lag far behind. Members of the Eop1 group are widely present in the Erwinia-Pantoea clade of Gram-negative bacteria, which contains phytopathogens, non-pathogens and potential biocontrol agents, implying that they may play an important role in agroecological or pathological adaptations. The lack of research in this group of YopJ effectors has left a significant knowledge gap in their functioning and role. For the first time, we perform a comparative analysis combining AlphaFold modelling, in planta transient expressions and targeted mutational analyses of the Eop1 group effectors from the Erwinia-Pantoea clade, to help elucidate their likely activity and mechanism(s). This integrated study revealed several new findings, including putative binding sites for inositol hexakisphosphate and acetyl coenzyme A and newly postulated target-binding domains, and raises questions about whether these effectors function through a catalytic triad mechanism. The results imply that some Eop1s may use a catalytic dyad acetylation mechanism that we found could be promoted by the electronegative environment around the active site.<\/jats:p>","DOI":"10.3390\/ijms241914664","type":"journal-article","created":{"date-parts":[[2023,9,28]],"date-time":"2023-09-28T01:42:12Z","timestamp":1695865332000},"page":"14664","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Structure-Function Characterisation of Eop1 Effectors from the Erwinia-Pantoea Clade Reveals They May Acetylate Their Defence Target through a Catalytic Dyad"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-2326-7875","authenticated-orcid":false,"given":"Vishant","family":"Tomar","sequence":"first","affiliation":[{"name":"Mt Albert Research Centre, The New Zealand Institute for Plant and Food Research Limited, Auckland 1025, New Zealand"},{"name":"School of Agriculture and Environment, Massey University, Private Bag 11222, Palmerston North 4442, New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4682-5078","authenticated-orcid":false,"given":"Erik H. A.","family":"Rikkerink","sequence":"additional","affiliation":[{"name":"Mt Albert Research Centre, The New Zealand Institute for Plant and Food Research Limited, Auckland 1025, New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Janghoon","family":"Song","sequence":"additional","affiliation":[{"name":"Pear Research Institute, National Institute of Horticultural & Herbal Science, Rural Development Administration, Naju 58216, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0121-8445","authenticated-orcid":false,"given":"Svetla","family":"Sofkova-Bobcheva","sequence":"additional","affiliation":[{"name":"School of Agriculture and Environment, Massey University, Private Bag 11222, Palmerston North 4442, New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4071-4457","authenticated-orcid":false,"given":"Vincent G. M.","family":"Bus","sequence":"additional","affiliation":[{"name":"Hawkes Bay Research Centre, The New Zealand Institute for Plant and Food Research Limited, Havelock North 4130, New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,9,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1038\/nature05286","article-title":"The plant immune system","volume":"444","author":"Jones","year":"2006","journal-title":"Nature"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1146\/annurev.arplant.57.032905.105346","article-title":"A renaissance of elicitors: Perception of microbe-associated molecular patterns and danger signals by pattern-recognition receptors","volume":"60","author":"Boller","year":"2009","journal-title":"Annu. Rev. 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