{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T21:09:56Z","timestamp":1773954596188,"version":"3.50.1"},"reference-count":49,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2023,9,28]],"date-time":"2023-09-28T00:00:00Z","timestamp":1695859200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Yangling Seed Industry Innovation Key Research and Development","award":["Ylzy-xm-03"],"award-info":[{"award-number":["Ylzy-xm-03"]}]},{"name":"Yangling Seed Industry Innovation Key Research and Development","award":["2018SZS-22"],"award-info":[{"award-number":["2018SZS-22"]}]},{"name":"Yangling Seed Industry Innovation Key Research and Development","award":["2452022109"],"award-info":[{"award-number":["2452022109"]}]},{"name":"Yangling Seed Industry Innovation Key Research and Development","award":["X202310712475"],"award-info":[{"award-number":["X202310712475"]}]},{"name":"Key Laboratory of Shaanxi Province","award":["Ylzy-xm-03"],"award-info":[{"award-number":["Ylzy-xm-03"]}]},{"name":"Key Laboratory of Shaanxi Province","award":["2018SZS-22"],"award-info":[{"award-number":["2018SZS-22"]}]},{"name":"Key Laboratory of Shaanxi Province","award":["2452022109"],"award-info":[{"award-number":["2452022109"]}]},{"name":"Key Laboratory of Shaanxi Province","award":["X202310712475"],"award-info":[{"award-number":["X202310712475"]}]},{"name":"Fundamental Research Funds for the Central Universities","award":["Ylzy-xm-03"],"award-info":[{"award-number":["Ylzy-xm-03"]}]},{"name":"Fundamental Research Funds for the Central Universities","award":["2018SZS-22"],"award-info":[{"award-number":["2018SZS-22"]}]},{"name":"Fundamental Research Funds for the Central Universities","award":["2452022109"],"award-info":[{"award-number":["2452022109"]}]},{"name":"Fundamental Research Funds for the Central Universities","award":["X202310712475"],"award-info":[{"award-number":["X202310712475"]}]},{"name":"Innovation and Entrepreneurship Training for College Students","award":["Ylzy-xm-03"],"award-info":[{"award-number":["Ylzy-xm-03"]}]},{"name":"Innovation and Entrepreneurship Training for College Students","award":["2018SZS-22"],"award-info":[{"award-number":["2018SZS-22"]}]},{"name":"Innovation and Entrepreneurship Training for College Students","award":["2452022109"],"award-info":[{"award-number":["2452022109"]}]},{"name":"Innovation and Entrepreneurship Training for College Students","award":["X202310712475"],"award-info":[{"award-number":["X202310712475"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Chlorophyll is an indispensable photoreceptor in plant photosynthesis. Its anabolic imbalance is detrimental to individual growth and development. As an essential epigenetic modification, DNA methylation can induce phenotypic variations, such as leaf color transformation, by regulating gene expression. Albino line XN1376B is a natural mutation of winter wheat cultivar XN1376; however, the regulatory mechanism of its albinism is still unclear. In this study, we found that low temperatures induced albinism in XN1376B. The number of chloroplasts decreased as the phenomenon of bleaching intensified and the fence tissue and sponge tissue slowly dissolved. We identified six distinct TaPOR (protochlorophyllide oxidoreductase) genes in the wheat genome, and TaPOR2D was deemed to be related to the phenomenon of albinism based on the expression in different color leaves (green leaves, white leaves and returned green leaves) and the analysis of promoters\u2019 cis-acting elements. TaPOR2D was localized to chloroplasts. TaPOR2D overexpression (TaPOR2D-OE) enhanced the chlorophyll significantly in Arabidopsis, especially at two weeks; the amount of chlorophyll was 6.46 mg\/L higher than in WT. The methylation rate of the TaPOR2D promoter in low-temperature albino leaves is as high as 93%, whereas there was no methylation in green leaves. Correspondingly, three DNA methyltransferase genes (TaMET1, TaDRM and TaCMT) were up-regulated in white leaves. Our study clarified that the expression of TaPOR2D is associated with its promoter methylation at a low temperature; it affects the level of chlorophyll accumulation, which probably causes the abnormal development of plant chloroplasts in albino wheat XN1376B. The results provide a theoretical basis for in-depth analysis of the regulation of development of plant chloroplasts and color variation in wheat XN1376B leaves.<\/jats:p>","DOI":"10.3390\/ijms241914697","type":"journal-article","created":{"date-parts":[[2023,9,28]],"date-time":"2023-09-28T05:55:54Z","timestamp":1695880554000},"page":"14697","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Low-Temperature-Mediated Promoter Methylation Relates to the Expression of TaPOR2D, Affecting the Level of Chlorophyll Accumulation in Albino Wheat (Triticum aestivum L.)"],"prefix":"10.3390","volume":"24","author":[{"given":"Jingjing","family":"Du","sequence":"first","affiliation":[{"name":"College of Agronomy, Northwest A & F University, Xianyang 712100, China"},{"name":"Key Laboratory of Crop Heterosis of Shaanxi Province, Xianyang 712100, China"},{"name":"Wheat Breeding Engineering Research Center of Ministry of Education, Xianyang 712100, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junwei","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Agronomy, Northwest A & F University, Xianyang 712100, China"},{"name":"Key Laboratory of Crop Heterosis of Shaanxi Province, Xianyang 712100, China"},{"name":"Wheat Breeding Engineering Research Center of Ministry of Education, Xianyang 712100, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sicong","family":"Shan","sequence":"additional","affiliation":[{"name":"College of Agronomy, Northwest A & F University, Xianyang 712100, China"},{"name":"Key Laboratory of Crop Heterosis of Shaanxi Province, Xianyang 712100, China"},{"name":"Wheat Breeding Engineering Research Center of Ministry of Education, Xianyang 712100, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tian","family":"Mi","sequence":"additional","affiliation":[{"name":"College of Agronomy, Northwest A & F University, Xianyang 712100, China"},{"name":"Key Laboratory of Crop Heterosis of Shaanxi Province, Xianyang 712100, China"},{"name":"Wheat Breeding Engineering Research Center of Ministry of Education, Xianyang 712100, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yulong","family":"Song","sequence":"additional","affiliation":[{"name":"College of Agronomy, Northwest A & F University, Xianyang 712100, China"},{"name":"Key Laboratory of Crop Heterosis of Shaanxi Province, Xianyang 712100, China"},{"name":"Wheat Breeding Engineering Research Center of Ministry of Education, Xianyang 712100, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu","family":"Xia","sequence":"additional","affiliation":[{"name":"College of Agronomy, Northwest A & F University, Xianyang 712100, China"},{"name":"Key Laboratory of Crop Heterosis of Shaanxi Province, Xianyang 712100, China"},{"name":"Wheat Breeding Engineering Research Center of Ministry of Education, Xianyang 712100, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shoucai","family":"Ma","sequence":"additional","affiliation":[{"name":"College of Agronomy, Northwest A & F University, Xianyang 712100, China"},{"name":"Key Laboratory of Crop Heterosis of Shaanxi Province, Xianyang 712100, China"},{"name":"Wheat Breeding Engineering Research Center of Ministry of Education, Xianyang 712100, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gaisheng","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Agronomy, Northwest A & F University, Xianyang 712100, China"},{"name":"Key Laboratory of Crop Heterosis of Shaanxi Province, Xianyang 712100, China"},{"name":"Wheat Breeding Engineering Research Center of Ministry of Education, Xianyang 712100, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lingjian","family":"Ma","sequence":"additional","affiliation":[{"name":"College of Agronomy, Northwest A & F University, Xianyang 712100, China"},{"name":"Key Laboratory of Crop Heterosis of Shaanxi Province, Xianyang 712100, China"},{"name":"Wheat Breeding Engineering Research Center of Ministry of Education, Xianyang 712100, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7955-0712","authenticated-orcid":false,"given":"Na","family":"Niu","sequence":"additional","affiliation":[{"name":"College of Agronomy, Northwest A & F University, Xianyang 712100, China"},{"name":"Key Laboratory of Crop Heterosis of Shaanxi Province, Xianyang 712100, China"},{"name":"Wheat Breeding Engineering Research Center of Ministry of Education, Xianyang 712100, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,9,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"6691","DOI":"10.1039\/C7TA11274B","article-title":"Supramolecular approach towards 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