{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T16:20:40Z","timestamp":1778170840782,"version":"3.51.4"},"reference-count":45,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2023,9,28]],"date-time":"2023-09-28T00:00:00Z","timestamp":1695859200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo (FAPESP)","award":["2018\/02537-5"],"award-info":[{"award-number":["2018\/02537-5"]}]},{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo (FAPESP)","award":["2019\/27807-8"],"award-info":[{"award-number":["2019\/27807-8"]}]},{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo (FAPESP)","award":["LIM-20 FMUSP"],"award-info":[{"award-number":["LIM-20 FMUSP"]}]},{"name":"Laboratory of Medical Investigations","award":["2018\/02537-5"],"award-info":[{"award-number":["2018\/02537-5"]}]},{"name":"Laboratory of Medical Investigations","award":["2019\/27807-8"],"award-info":[{"award-number":["2019\/27807-8"]}]},{"name":"Laboratory of Medical Investigations","award":["LIM-20 FMUSP"],"award-info":[{"award-number":["LIM-20 FMUSP"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The synthesized peptide derived from Enterolobium contortisiliquum (pep3-EcTI) has been associated with potent anti-inflammatory and antioxidant effects, and it may be a potential new treatment for asthma\u2013COPD overlap\u2014ACO). Purpose: To investigate the primary sequence effects of pep3-EcTI in an experimental ACO. BALB\/c mice were divided into eight groups: SAL (saline), OVA (ovalbumin), ELA (elastase), ACO (ovalbumin + elastase), ACO-pep3-EcTI (treated with inhibitor), ACO-DX (treated with dexamethasone), ACO-DX-pep3-EcTI (treated with dexamethasone and inhibitor), and SAL-pep3-EcTI (saline group treated with inhibitor). We evaluated the hyperresponsiveness to methacholine, exhaled nitric oxide, bronchoalveolar lavage fluid (BALF), mean linear intercept (Lm), inflammatory markers, tumor necrosis factor (TNF-\u03b1), interferon (IFN)), matrix metalloproteinases (MMPs), growth factor (TGF-\u03b2), collagen fibers, the oxidative stress marker inducible nitric oxide synthase (iNOS), transcription factors, and the signaling pathway NF-\u03baB in the airways (AW) and alveolar septa (AS). Statistical analysis was conducted using one-way ANOVA and t-tests, significant when p &lt; 0.05. ACO caused alterations in the airways and alveolar septa. Compared with SAL, ACO-pep3-EcTI reversed the changes in the percentage of resistance of the respiratory system (%Rrs), the elastance of the respiratory system (%Ers), tissue resistance (%Gtis), tissue elastance (%Htis), airway resistance (%Raw), Lm, exhaled nitric oxide (ENO), lymphocytes, IL-4, IL-5, IL-6, IL-10, IL-13, IL-17, TNF-\u03b1, INF-\u03b3, MMP-12, transforming growth factor (TGF)-\u03b2, collagen fibers, and iNOS. ACO-DX reversed the changes in %Rrs, %Ers, %Gtis, %Htis, %Raw, total cells, eosinophils, neutrophils, lymphocytes, macrophages, IL-1\u03b2, IL-6, IL-10, IL-13, IL-17, TNF-\u03b1, INF-\u03b3, MMP-12, TGF-\u03b2, collagen fibers, and iNOS. ACO-DX-pep3-EcTI reversed the changes, as was also observed for the pep3-EcTI and the ACO-DX-pep3-EcTI. Significance: The pep3-EcTI was revealed to be a promising strategy for the treatment of ACO, asthma, and COPD.<\/jats:p>","DOI":"10.3390\/ijms241914710","type":"journal-article","created":{"date-parts":[[2023,9,29]],"date-time":"2023-09-29T02:47:30Z","timestamp":1695955650000},"page":"14710","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Investigating the Effects of a New Peptide, Derived from the Enterolobium contortisiliquum Proteinase Inhibitor (EcTI), on Inflammation, Remodeling, and Oxidative Stress in an Experimental Mouse Model of Asthma\u2013Chronic Obstructive Pulmonary Disease Overlap (ACO)"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7378-9485","authenticated-orcid":false,"given":"J\u00e9ssica Anast\u00e1cia Silva","family":"Barbosa","sequence":"first","affiliation":[{"name":"Faculdade de Medicina FMUSP, Universidade de S\u00e3o Paulo, S\u00e3o Paulo 01246-903, Brazil"},{"name":"Hospital S\u00edrio Liban\u00eas, S\u00e3o Paulo 01308-050, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1321-1942","authenticated-orcid":false,"given":"Luana Laura Sales","family":"da Silva","sequence":"additional","affiliation":[{"name":"Faculdade de Medicina FMUSP, Universidade de S\u00e3o Paulo, S\u00e3o Paulo 01246-903, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juliana Morelli Lopes Gon\u00e7alves","family":"Jo\u00e3o","sequence":"additional","affiliation":[{"name":"Faculdade de Medicina FMUSP, Universidade de S\u00e3o Paulo, S\u00e3o Paulo 01246-903, Brazil"},{"name":"Hospital S\u00edrio Liban\u00eas, S\u00e3o Paulo 01308-050, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elaine Cristina","family":"de Campos","sequence":"additional","affiliation":[{"name":"Faculdade de Medicina FMUSP, Universidade de S\u00e3o Paulo, S\u00e3o Paulo 01246-903, Brazil"},{"name":"Hospital S\u00edrio Liban\u00eas, S\u00e3o Paulo 01308-050, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvia","family":"Fukuzaki","sequence":"additional","affiliation":[{"name":"Faculdade de Medicina FMUSP, Universidade de S\u00e3o Paulo, S\u00e3o Paulo 01246-903, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leandro do Nascimento","family":"Camargo","sequence":"additional","affiliation":[{"name":"Faculdade de Medicina FMUSP, Universidade de S\u00e3o Paulo, S\u00e3o Paulo 01246-903, Brazil"},{"name":"Hospital S\u00edrio Liban\u00eas, S\u00e3o Paulo 01308-050, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tabata Maruyama","family":"dos Santos","sequence":"additional","affiliation":[{"name":"Faculdade de Medicina FMUSP, Universidade de S\u00e3o Paulo, S\u00e3o Paulo 01246-903, Brazil"},{"name":"Hospital S\u00edrio Liban\u00eas, S\u00e3o Paulo 01308-050, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henrique Tibucheski","family":"dos Santos","sequence":"additional","affiliation":[{"name":"Faculdade de Medicina FMUSP, Universidade de S\u00e3o Paulo, S\u00e3o Paulo 01246-903, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suellen Karoline Moreira","family":"Bezerra","sequence":"additional","affiliation":[{"name":"Faculdade de Medicina FMUSP, Universidade de S\u00e3o Paulo, S\u00e3o Paulo 01246-903, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beatriz Mangueira","family":"Saraiva-Romanholo","sequence":"additional","affiliation":[{"name":"Faculdade de Medicina FMUSP, Universidade de S\u00e3o Paulo, S\u00e3o Paulo 01246-903, Brazil"},{"name":"Department of Medicine, University City of S\u00e3o Paulo, S\u00e3o Paulo 03071-000, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6402-3055","authenticated-orcid":false,"given":"Fernanda Degobbi Ten\u00f3rio Quirino dos Santos","family":"Lopes","sequence":"additional","affiliation":[{"name":"Faculdade de Medicina FMUSP, Universidade de S\u00e3o Paulo, S\u00e3o Paulo 01246-903, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9324-8424","authenticated-orcid":false,"given":"Camila Ramalho","family":"Bonturi","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica, Universidade Federal de S\u00e3o Paulo (UNIFESP), S\u00e3o Paulo 04039-002, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7346-6328","authenticated-orcid":false,"given":"Maria Luiza Vilela","family":"Oliva","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica, Universidade Federal de S\u00e3o Paulo (UNIFESP), S\u00e3o Paulo 04039-002, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edna Aparecida","family":"Leick","sequence":"additional","affiliation":[{"name":"Faculdade de Medicina FMUSP, Universidade de S\u00e3o Paulo, S\u00e3o Paulo 01246-903, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6234-1458","authenticated-orcid":false,"given":"Renato Fraga","family":"Righetti","sequence":"additional","affiliation":[{"name":"Faculdade de Medicina FMUSP, Universidade de S\u00e3o Paulo, S\u00e3o Paulo 01246-903, Brazil"},{"name":"Hospital S\u00edrio Liban\u00eas, S\u00e3o Paulo 01308-050, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Iolanda de F\u00e1tima Lopes Calvo","family":"Tib\u00e9rio","sequence":"additional","affiliation":[{"name":"Faculdade de Medicina FMUSP, Universidade de S\u00e3o Paulo, S\u00e3o Paulo 01246-903, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,9,28]]},"reference":[{"key":"ref_1","unstructured":"(2023, July 15). 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