{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,30]],"date-time":"2026-08-30T13:19:59Z","timestamp":1788095999915,"version":"build-2803163510"},"reference-count":90,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2023,9,30]],"date-time":"2023-09-30T00:00:00Z","timestamp":1696032000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012706","name":"University of Warmia and Mazury in Olsztyn","doi-asserted-by":"publisher","award":["11.610.003-300"],"award-info":[{"award-number":["11.610.003-300"]}],"id":[{"id":"10.13039\/501100012706","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012706","name":"University of Warmia and Mazury in Olsztyn","doi-asserted-by":"publisher","award":["010\/RID\/2018\/19"],"award-info":[{"award-number":["010\/RID\/2018\/19"]}],"id":[{"id":"10.13039\/501100012706","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Minister of Education and Science in the range of the program entitled \u201cRegional Initiative of Excellence\u201d for the years 2019-2023","award":["11.610.003-300"],"award-info":[{"award-number":["11.610.003-300"]}]},{"name":"Minister of Education and Science in the range of the program entitled \u201cRegional Initiative of Excellence\u201d for the years 2019-2023","award":["010\/RID\/2018\/19"],"award-info":[{"award-number":["010\/RID\/2018\/19"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Sperm maturation in the epididymis is based on interactions with proteins from epididymal fluid (EF). The aim of the study was to profile canine EF proteome and investigate correlations between EF protein content and epididymal spermatozoa (ES) motion parameters. Twenty-three male dogs were divided into two groups: good sperm motility (GSM) and poor sperm motility (PSM). The total motility and progressive motility differed significantly (p = 0.031; p &lt; 0.001, respectively) between the GSM group and the PSM group. The semen samples were centrifuged to separate the EF apart from the ES. The canine EF proteins were analyzed using nano-liquid chromatography, which was coupled with quadrupole time-of-flight mass spectrometry (NanoUPLC-Q-TOF\/MS) and bioinformatic tools for the first time. A total of 915 proteins were identified (GSM\u2014506; PSM\u2014409, respectively). UniProt identification resulted in six unique proteins (UPs) in the GSM group of dogs and four UPs in the PSM group. A semi-quantitative analysis showed a higher abundance (p &lt; 0.05) of four differentially expressed proteins in the GSM group (ALB, CRISP2, LCNL1, PTGDS). Motility-dependent variations were detected in the EF proteome and were related to important metabolic pathways, which might suggest that several proteins could be potential ES motility biomarkers.<\/jats:p>","DOI":"10.3390\/ijms241914790","type":"journal-article","created":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T16:58:09Z","timestamp":1696179489000},"page":"14790","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Proteome Profiling of Canine Epididymal Fluid: In Search of Protein Markers of Epididymal Sperm Motility"],"prefix":"10.3390","volume":"24","author":[{"given":"Aleksandra W.","family":"Cichowska","sequence":"first","affiliation":[{"name":"Department of Animal Biochemistry and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 5, 10-719 Olsztyn, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jerzy","family":"Wisniewski","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Molecular Biology and Biotechnology, Wroclaw University of Science and Technology, Wyspianskiego 27, 50-370 Wroclaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0806-6608","authenticated-orcid":false,"given":"Mariusz A.","family":"Bromke","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Immunochemistry, Wroclaw Medical University, Chalubinskiego 10, 50-368 Wroclaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1706-177X","authenticated-orcid":false,"given":"Beata","family":"Olejnik","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Immunochemistry, Wroclaw Medical University, Chalubinskiego 10, 50-368 Wroclaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6611-2740","authenticated-orcid":false,"given":"Marzena","family":"Mogielnicka-Brzozowska","sequence":"additional","affiliation":[{"name":"Department of Animal Biochemistry and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 5, 10-719 Olsztyn, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,9,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"R155","DOI":"10.1530\/REP-18-0589","article-title":"The impact of epididymal proteins on sperm function","volume":"158","year":"2019","journal-title":"Reproduction"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1234","DOI":"10.21451\/1984-3143-AR955","article-title":"The epididymis and its role on sperm quality and male fertility","volume":"14","author":"Lucca","year":"2017","journal-title":"Anim. 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