{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,17]],"date-time":"2026-01-17T22:32:03Z","timestamp":1768689123233,"version":"3.49.0"},"reference-count":52,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2023,9,30]],"date-time":"2023-09-30T00:00:00Z","timestamp":1696032000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Institut Pasteur","award":["UMR 3569"],"award-info":[{"award-number":["UMR 3569"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Infection with Ebola virus (EBOV) is responsible for hemorrhagic fever in humans with a high mortality rate. Combined efforts of prevention and therapeutic intervention are required to tackle highly variable RNA viruses, whose infections often lead to outbreaks. Here, we have screened the 2P2I3D chemical library using a nanoluciferase-based protein complementation assay (NPCA) and isolated two compounds that disrupt the interaction of the EBOV protein fragment VP35IID with the N-terminus of the dsRNA-binding proteins PKR and PACT, involved in IFN response and\/or intrinsic immunity, respectively. The two compounds inhibited EBOV infection in cell culture as well as infection by measles virus (MV) independently of IFN induction. Consequently, we propose that the compounds are antiviral by restoring intrinsic immunity driven by PACT. Given that PACT is highly conserved across mammals, our data support further testing of the compounds in other species, as well as against other negative-sense RNA viruses.<\/jats:p>","DOI":"10.3390\/ijms241914791","type":"journal-article","created":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T16:58:09Z","timestamp":1696179489000},"page":"14791","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Novel Antiviral Molecules against Ebola Virus Infection"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2304-6402","authenticated-orcid":false,"given":"Mila","family":"Collados Rodr\u00edguez","sequence":"first","affiliation":[{"name":"School of Infection & Immunity (SII), College of Medical, Veterinary and Life Sciences (MVLS), Sir Michael Stoker Building, MRC-University of Glasgow Centre for Virus Research (CVR), Glasgow G61 1QH, UK"},{"name":"Unit\u00e9 H\u00e9pacivirus et Immunit\u00e9 Inn\u00e9e, CNRS, UMR 3569, D\u00e9partement de Virologie, Institut Pasteur, 75015 Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrick","family":"Maillard","sequence":"additional","affiliation":[{"name":"Unit\u00e9 H\u00e9pacivirus et Immunit\u00e9 Inn\u00e9e, CNRS, UMR 3569, D\u00e9partement de Virologie, Institut Pasteur, 75015 Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexandra","family":"Journeaux","sequence":"additional","affiliation":[{"name":"Unit of Biology of Emerging Viral Infections, Institut Pasteur, 69007 Lyon, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9998-8447","authenticated-orcid":false,"given":"Anastassia V.","family":"Komarova","sequence":"additional","affiliation":[{"name":"Interactomics, RNA and Immunity Laboratory, Institut Pasteur, 75015 Paris, France"},{"name":"Unit\u00e9 de G\u00e9nomique Virale et Vaccination, Institut Pasteur, 75015 Paris, France"},{"name":"Universit\u00e9 Paris Cit\u00e9, 75013 Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Val\u00e9rie","family":"Najburg","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de G\u00e9nomique Virale et Vaccination, Institut Pasteur, 75015 Paris, France"},{"name":"Universit\u00e9 Paris Cit\u00e9, 75013 Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raul-Yusef Sanchez","family":"David","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de G\u00e9nomique Virale et Vaccination, Institut Pasteur, 75015 Paris, France"},{"name":"Blizard Institute\u2014Faculty of Medicine and Dentistry, Queen Mary University of London, London E1 2AT, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olivier","family":"Helynck","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Chimie et Biocatalyse, CNRS, UMR 3523, Institut Pasteur, Universit\u00e9 de Paris, 75015 Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mingzhe","family":"Guo","sequence":"additional","affiliation":[{"name":"CAS Key Laboratory of Molecular Virology and Immunology, Unit of Viral Hepatitis, Shanghai Institute of Immunity and Infection, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Shanghai 200023, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jin","family":"Zhong","sequence":"additional","affiliation":[{"name":"CAS Key Laboratory of Molecular Virology and Immunology, Unit of Viral Hepatitis, Shanghai Institute of Immunity and Infection, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Shanghai 200023, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3234-7477","authenticated-orcid":false,"given":"Sylvain","family":"Baize","sequence":"additional","affiliation":[{"name":"Unit of Biology of Emerging Viral Infections, Institut Pasteur, 69007 Lyon, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fr\u00e9d\u00e9ric","family":"Tangy","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de G\u00e9nomique Virale et Vaccination, Institut Pasteur, 75015 Paris, France"},{"name":"Universit\u00e9 Paris Cit\u00e9, 75013 Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yves","family":"Jacob","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Cit\u00e9, 75013 Paris, France"},{"name":"Unit\u00e9 G\u00e9n\u00e9tique Mol\u00e9culaire des Virus \u00e0 ARN, CNRS, UMR 3569, D\u00e9partement de Virologie, Institut Pasteur, 75015 Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7579-8561","authenticated-orcid":false,"given":"H\u00e9l\u00e8ne","family":"Munier-Lehmann","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Chimie et Biocatalyse, CNRS, UMR 3523, Institut Pasteur, Universit\u00e9 de Paris, 75015 Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eliane F.","family":"Meurs","sequence":"additional","affiliation":[{"name":"Unit\u00e9 H\u00e9pacivirus et Immunit\u00e9 Inn\u00e9e, CNRS, UMR 3569, D\u00e9partement de Virologie, Institut Pasteur, 75015 Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,9,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Jain, S., Khaiboullina, S., Martynova, E., Morzunov, S., and Baranwal, M. 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