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Oxidative stress is one of the primary causes of quality decline in oocytes matured in vitro. In this study, ferulic acid (FA), a natural antioxidant found in plant cell walls, was investigated to evaluate its impact on bovine oocyte maturation and subsequent embryonic development. Bovine cumulus\u2013oocyte complexes (COCs) were treated with different concentrations of FA (0, 2.5, 5, 10, 20 \u03bcM) during in vitro maturation (IVM). Compared to the control group, supplementation with 5 \u03bcM FA significantly enhanced the maturation rates of bovine oocytes and the expansion of the cumulus cells area, as well as the subsequent cleavage and blastocyst formation rates after in vitro fertilization (IVF) and somatic cell nuclear transfer (SCNT). Furthermore, FA supplementation was observed to effectively decrease the levels of ROS in bovine oocytes and improve their mitochondrial function. Our experiments demonstrate that FA can maintain the levels of antioxidants (GSH, SOD, CAT) in oocytes, thereby alleviating the oxidative stress induced by H2O2. RT-qPCR results revealed that, after FA treatment, the relative mRNA expression levels of genes related to oocyte maturation (GDF-9 and BMP-15), cumulus cell expansion (HAS2, PTX3, CX37, and CX43), and embryo pluripotency (OCT4, SOX2, and CDX2) were significantly increased. In conclusion, these findings demonstrate that FA supplementation during bovine oocyte IVM can enhance oocyte quality and the developmental potential of subsequent embryos.<\/jats:p>","DOI":"10.3390\/ijms241914804","type":"journal-article","created":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T16:58:09Z","timestamp":1696179489000},"page":"14804","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Ferulic Acid Enhances Oocyte Maturation and the Subsequent Development of Bovine Oocytes"],"prefix":"10.3390","volume":"24","author":[{"given":"Yu","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Animals Sciences, College of Animal Sciences, Jilin University, Changchun 130062, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia-Jia","family":"Qi","sequence":"additional","affiliation":[{"name":"Department of Animals Sciences, College of Animal Sciences, Jilin University, Changchun 130062, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi-Jing","family":"Yin","sequence":"additional","affiliation":[{"name":"Department of Animals Sciences, College of Animal Sciences, Jilin University, Changchun 130062, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2008-6166","authenticated-orcid":false,"given":"Hao","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Animals Sciences, College of Animal Sciences, Jilin University, Changchun 130062, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia-Bao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Animals Sciences, College of Animal Sciences, Jilin University, Changchun 130062, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2063-1662","authenticated-orcid":false,"given":"Shuang","family":"Liang","sequence":"additional","affiliation":[{"name":"Department of Animals Sciences, College of Animal Sciences, Jilin University, Changchun 130062, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3490-0755","authenticated-orcid":false,"given":"Bao","family":"Yuan","sequence":"additional","affiliation":[{"name":"Department of Animals Sciences, College of Animal Sciences, Jilin University, Changchun 130062, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,9,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1590","DOI":"10.2527\/2003.8161590x","article-title":"Use of insulin-like growth factor-I during embryo culture and treatment of recipients with gonadotropin-releasing hormone to increase pregnancy rates following the transfer of in vitro-produced embryos to heat-stressed, lactating cows","volume":"81","author":"Block","year":"2003","journal-title":"J. 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