{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:48:41Z","timestamp":1787028521733,"version":"build-2736575974"},"reference-count":62,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2023,10,5]],"date-time":"2023-10-05T00:00:00Z","timestamp":1696464000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Italian Ministry of Education, University and Research","award":["IND2019\/BIO-17192 to JQT and AECC predoctoral fellowship 2022 to NAA"],"award-info":[{"award-number":["IND2019\/BIO-17192 to JQT and AECC predoctoral fellowship 2022 to NAA"]}]},{"name":"FSC","award":["IND2019\/BIO-17192 to JQT and AECC predoctoral fellowship 2022 to NAA"],"award-info":[{"award-number":["IND2019\/BIO-17192 to JQT and AECC predoctoral fellowship 2022 to NAA"]}]},{"name":"Industrial PhD programm Comunidad de Madrid","award":["IND2019\/BIO-17192 to JQT and AECC predoctoral fellowship 2022 to NAA"],"award-info":[{"award-number":["IND2019\/BIO-17192 to JQT and AECC predoctoral fellowship 2022 to NAA"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Numerous studies have reported the pharmacological effects exhibited by Dittrichia viscosa, (D. viscosa) including antioxidant, cytotoxic, antiproliferative, and anticancer properties. In our research, our primary objective was to validate a prescreening methodology aimed at identifying the fraction that demonstrates the most potent antiproliferative and anticancer effects. Specifically, we investigated the impact of various extract fractions on the cytoskeleton using a screening method involving transgenic plants. Tumors are inherently heterogeneous, and the components of the cytoskeleton, particularly tubulin, are considered a strategic target for antitumor agents. To take heterogeneity into account, we used different lines of colorectal cancer, specifically one of the most common cancers regardless of gender. In patients with metastasis, the effectiveness of chemotherapy has been limited by severe side effects and by the development of resistance. Additional therapies and antiproliferative molecules are therefore needed. In our study, we used colon-like cell lines characterized by the expression of gastrointestinal differentiation markers (such as the HT-29 cell line) and undifferentiated cell lines showing the positive regulation of epithelial\u2013mesenchymal transition and TGF\u03b2 signatures (such as the DLD-1, SW480, and SW620 cell lines). We showed that all three of the D. viscosa extract fractions have an antiproliferative effect but the pre-screening on transgenic plants anticipated that the methanolic fraction may be the most promising, targeting the cytoskeleton specifically and possibly resulting in fewer side effects. Here, we show that the preliminary use of screening in transgenic plants expressing subcellular markers can significantly reduce costs and focus the advanced characterization only on the most promising therapeutic molecules.<\/jats:p>","DOI":"10.3390\/ijms241914920","type":"journal-article","created":{"date-parts":[[2023,10,5]],"date-time":"2023-10-05T09:14:22Z","timestamp":1696497262000},"page":"14920","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Methanolic Extracts of D. viscosa Specifically Affect the Cytoskeleton and Exert an Antiproliferative Effect on Human Colorectal Cancer Cell Lines, According to Their Proliferation Rate"],"prefix":"10.3390","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4218-2706","authenticated-orcid":false,"given":"Chiara","family":"Anglana","sequence":"first","affiliation":[{"name":"Department of Biological and Environmental Sciences and Technologies (Di.S.Te.B.A.), University of Salento, Via Monteroni, 73100 Lecce, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3546-7765","authenticated-orcid":false,"given":"Makarena","family":"Rojas","sequence":"additional","affiliation":[{"name":"Department of Biological and Environmental Sciences and Technologies (Di.S.Te.B.A.), University of Salento, Via Monteroni, 73100 Lecce, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0085-8539","authenticated-orcid":false,"given":"Chiara Roberta","family":"Girelli","sequence":"additional","affiliation":[{"name":"Department of Biological and Environmental Sciences and Technologies (Di.S.Te.B.A.), University of Salento, Via Monteroni, 73100 Lecce, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1409-2324","authenticated-orcid":false,"given":"Fabrizio","family":"Barozzi","sequence":"additional","affiliation":[{"name":"Department of Biological and Environmental Sciences and Technologies (Di.S.Te.B.A.), University of Salento, Via Monteroni, 73100 Lecce, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1737-4186","authenticated-orcid":false,"given":"Josefa","family":"Quiroz-Troncoso","sequence":"additional","affiliation":[{"name":"Oncology Group IDISSC and Biomedical Technology Centre (CTB), Biotecnology-B.V. Departament ETSIAAB, Universidad Polit\u00e9cnica de Madrid, 28223 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5708-997X","authenticated-orcid":false,"given":"Nicol\u00e1s","family":"Alegr\u00eda-Aravena","sequence":"additional","affiliation":[{"name":"Oncology Group IDISSC and Biomedical Technology Centre (CTB), Biotecnology-B.V. Departament ETSIAAB, Universidad Polit\u00e9cnica de Madrid, 28223 Madrid, Spain"},{"name":"Deer Production and Biology Group, Regional Development Institute, University of Castilla-La Mancha, 02006 Albacete, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Montefusco","sequence":"additional","affiliation":[{"name":"Department of Biological and Environmental Sciences and Technologies (Di.S.Te.B.A.), University of Salento, Via Monteroni, 73100 Lecce, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1779-6426","authenticated-orcid":false,"given":"Miriana","family":"Durante","sequence":"additional","affiliation":[{"name":"Institute of Sciences of Food Production (ISPA-CNR), 73100 Lecce, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3073-5772","authenticated-orcid":false,"given":"Francesco Paolo","family":"Fanizzi","sequence":"additional","affiliation":[{"name":"Department of Biological and Environmental Sciences and Technologies (Di.S.Te.B.A.), University of Salento, Via Monteroni, 73100 Lecce, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1877-3381","authenticated-orcid":false,"given":"Carmen","family":"Ram\u00edrez-Castillejo","sequence":"additional","affiliation":[{"name":"Oncology Group IDISSC and Biomedical Technology Centre (CTB), Biotecnology-B.V. Departament ETSIAAB, Universidad Polit\u00e9cnica de Madrid, 28223 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5388-0695","authenticated-orcid":false,"given":"Gian-Pietro","family":"Di Sansebastiano","sequence":"additional","affiliation":[{"name":"Department of Biological and Environmental Sciences and Technologies (Di.S.Te.B.A.), University of Salento, Via Monteroni, 73100 Lecce, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,10,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Khan, T., Ali, M., Khan, A., Nisar, P., Jan, S.A., Afridi, S., and Shinwari, Z.K. (2019). Anticancer Plants: A Review of the Active Phytochemicals, Applications in Animal Models, and Regulatory Aspects. 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